distribution of na k atpase in photoreceptor cells of insects

... functions of the NKA isoforms in skeletal muscle NKA Function isoform α1 Key housekeeping isoform, large contribution to Na+ /K+ exchange mainly during basal conditions α2 Key α isoform in skeletal ... effects of a longitudinal training program on NKA isoform abundance in human are unknown Therefore Chapter investigated whether 12 weeks of high-intensity interval training (HIT) in aged individuals ... 16% (McKenna et al., 1993) No changes in muscle [3H]ouabain binding or NKA isoform abundance were seen in elite cyclists completing additional interval training involving x at 80% of peak power...

Ngày tải lên: 04/12/2015, 13:59

... cascade of events Based mainly on observations with rat myocytes, additional roles in signal-transducing function of ouabain’s interaction with Na+ /K+ -ATPase were attributed to tyrosine protein kinase, ... elicited by the interaction of a minor fraction of ouabainaltered Na+ /K+ -ATPase with neighbouring proteins (Src, growth factor receptors and Ras), i.e by protein–protein interaction In myocytes, ... obtain Ca2+-oscillations in a reasonable fraction of the rat kidney cells [8], though oscillations were seen in a few cells exposed to 10–100 nM ouabain for h As ouabain binding to Na+ /K+ -ATPase...

Ngày tải lên: 23/03/2014, 17:21

... Mapping of ATP and pNPP binding sites of the Na pump (Eur J Biochem 271) 3933 Table Comparison of the primary structures of isoforms of Na+ /K+ ATPase and H+ /K+ ATPase in the range of the binding ... nucleotide binding sites in Na, K- ATPase In Na/ KATPase and Related ATPases (Taniguchi, K & Kaya, S., eds), pp 115122 Elsevier Science, Amsterdam ể FEBS 2004 59 Linnertz, H., Miksik, I., Kvasnicka, P., ... loop protein of Na+ /K+ ATPase [55] In conclusion, neither method revealed any indication of a conformational change of the N-domain upon ligand binding in the investigated GST fusion protein It is...

Ngày tải lên: 19/02/2014, 16:20

... affinity of Na+ ⁄ K+ -ATPase for Na+ [14] The resultant increase in Na+ binding was regarded as Na+ occlusion In the absence of oligomycin, NaCl gradually inhibited pNPPase activity and Rb+ binding ... responsible for oligomycin-induced occlusion of Na+ within Na ⁄ K- ATPase J Biol Chem 271, 25604–25610 18 Homareda H, Ishii T & Takeyasu K (2000) Binding domain of oligomycin on Na+ ⁄ K+ -ATPase Eur J Pharmacol ... for K+ [38] Increasing the KCl concentration in the presence of NaCl and oligomycin induces the activation curve with two phases (Fig 3) As K+ binding competes with Na+ binding [15], it is likely...

Ngày tải lên: 07/03/2014, 16:20

... subunit of Na+ /K+ ATPase in HEK293 cells [24,25] In Sf9 cells, however, expression of H+ /K+ -ATPase a subunit without a corresponding b subunit produces a ouabain-insensitive H+ /K+ ATPase [26] Finally, ... (Fig 2) The inhibition of palytoxin-induced currents by vanadate, a known blocker of apical H+ /K+ -ATPase [22], suggests that this enzyme is involved in the formation of the palytoxininduced channels ... latter findings, as the palytoxin-induced conductance was insensitive to mM ouabain while being inhibited at low concentrations of orthovanadate Similar findings showing a palytoxin-induced K+ efflux...

Ngày tải lên: 17/03/2014, 11:20

... irreversible tritium incorporation into Na+ /K+ -ATPase are shown in Fig Tritium incor- Fig E€ect of pressure on tritium incorporation into membrane-bound Na+ /K+ -ATPase Membrane-bound Na+ /K+ -ATPase was mixed ... emission maximum (kmax) and intensity of intrinsic ¯uorescence of Na+ /K+ -ATPase under increasing pressures are shown in Fig 2A The value for kmax was shifted from 341 to 345 nm with increasing pressure, ... forms Effects of pressure on ¯uorescence probes Fig E€ect of pressure on the intensity and kmax of intrinsic ¯uorescence of membrane-bound Na+ /K+ -ATPase (A) and solubilized Na+ / K+ -ATPase (B) Ten...

Ngày tải lên: 24/03/2014, 00:21

... supernatants were taken for scintillation counting Creatine kinase determination Creatine kinase activity was assayed following the method of Bernt and Bergmeyer [24], in the presence of fructose (50 ... prevention of aggregation of insulin B chain following reduction of disulphide bonds [13], refolding of guanidine hydrochloride (or urea)-denatured proteins [12,14] and prevention of inactivation of ... retained In the present study we demonstrate the protection of the membrane enzyme Na/ K- ATPase from inactivation by the heat shock protein a-crystallin Na/ K- ATPase activity decreased upon incubation...

Ngày tải lên: 17/03/2014, 03:20

... activation of p42/44 MAPK (also called ERK1/2) through the Ras/Raf/ MAPK kinase/MAPK cascade (where Raf is a MAPK kinase kinase and MEK is a MAPK kinase) [29,30] However, the activation of this ... In such cells, using high ouabain concentrations, or palytoxin, which also alters [Na+ ]i/ [K+ ]i ratio by interaction with Na+ /K+ -ATPase, activation of a number of protein kinase signaling pathways ... already clear that linkage to signaling intermediates and pathways through protein–protein interactions is a common property of Na+ /K+ -ATPase in most, if not all, cells Using partially inhibitory concentrations...

Ngày tải lên: 24/03/2014, 00:21

... organosomatic indices of west ern king prawn (Penaeus latisulcatus Kishinouye, 1896) reared at di fferent salinities Aquaculture 234:601-614 Wilder M.N., K Ikuta, M Atmomarsono, T Hatta and K Komoro ... C.R Emberson, D.A Davis, D McIntosh, A.L Lawrence and P.M Van Wyk 2002 Review of some recent developments in sustainable shrimp farming practices in Texas, Arizona and Florida J.Appl Aquac 12 ... regulation, In: Mantel L (Ed.) The biology of crustacean, Vol Internal anatomy and physiological regulation New York: Academic Press, pp:54-161 Menz A and B.F Blake 1980 Experiments on the growth of Penaeus...

Ngày tải lên: 02/04/2014, 10:20

... organosomatic indices of west ern king prawn (Penaeus latisulcatus Kishinouye, 1896) reared at di fferent salinities Aquaculture 234:601-614 Wilder M.N., K Ikuta, M Atmomarsono, T Hatta and K Komoro ... C.R Emberson, D.A Davis, D McIntosh, A.L Lawrence and P.M Van Wyk 2002 Review of some recent developments in sustainable shrimp farming practices in Texas, Arizona and Florida J.Appl Aquac 12 ... regulation, In: Mantel L (Ed.) The biology of crustacean, Vol Internal anatomy and physiological regulation New York: Academic Press, pp:54-161 Menz A and B.F Blake 1980 Experiments on the growth of Penaeus...

Ngày tải lên: 22/07/2014, 11:21

... Review of Nutrition, 1990;10:23354 Minassian DC, Mehra V Blinded by cataract: Each year projection from the first epidemiological study of incidence of cataract blindness in India British Journal of ... groups were both examined by an expert ophthalmologist and routine ophthalmic examinations including visual acuity determination, slit lamp examination, ophthalmoloscopy and intraocular pressure ... alterations in serum cation concentration, can be known as a risk factor for cataract formation Na+ pump activity in lens is as in other cells and it is related to intracellular Na+ , extracellular K+ ...

Ngày tải lên: 03/11/2012, 09:49

... localization of iNOS to apical domains of intestinal epithelial cells Particulate fraction association of iNOS in vivo In order to determine the distribution of iNOS in intestinal epithelial cells in vivo, ... Apical distribution of iNOS in intestinal epithelial cells To get more insight into the localization of iNOS in intestinal cells, Caco-2 cells were investigated Caco-2 FEBS Journal 272 (2005) 444–453 ... Subcellular distribution of iNOS in human cultured intestinal cells DLD-1 cells were incubated with cytokines for 14 h before cell fractionation (A) Distribution of Na+ ⁄ K+ -ATPase or LDH in cytosol...

Ngày tải lên: 19/02/2014, 16:20

... chemical covalent cross-linking agent J Biol Chem 263, 3290– 3295 21 Sorkina, T., Huang, F., Beguinot, L & Sorkin, A (2002) Effect of tyrosine kinase inhibitors on clathrin-coated pit recruitment ... trans-Golgi network of CHO -K1 cells Biochem J 342, 633–640 19 Friedrichson, T & Kurzchalia, T.V (1998) Microdomains of GPI-anchored proteins in living cells revealed by crosslinking Nature 394, 802–805 ... we analysed the heterologous expression of EGFr in CHO -K1 cells As expected, EGFr is expressed in CHO -K1 cells as a functional protein of 170 kDa [10] Endogenous EGFr expression in CHO -K1 cells...

Ngày tải lên: 16/03/2014, 16:20

... length k of the filling is a selection of rows r1 < · · · < rk and columns c1 < · · · < ck such that the cells (ri , ci ) are nonempty A ne chain of length k of the filling is a selection of rows ... g, l, m are in the filling The remaining two cells must be chosen one from each of the pairs {h, p}, {k, q} Of the four possible combinations, there is one that contains a nechain of length 2, ... A de Mier, k- noncrossing and k- nonnesting graphs and fillings of Ferrers diagrams, arXiv:math.CO/0602195 v2 [4] M Rubey, Increasing and decreasing sequences in fillings of moon polyominoes, arXiv:math.CO/0604140...

Ngày tải lên: 07/08/2014, 13:21

... 50% T cells in the tissue area; +++, >75% T cells in the tissue area cAverage numbers of CD4+CD28null T cells in one to five analysed infiltrates In six of the 13 patients analysed, the dominant ... excluded using the combination of CD3 and CD4 in the three-colour stainings to identify CD4+ T cells The density of CD4+CD28null T cells were calculated by dividing the number of CD4+CD28null T cells ... A, Ottenhoff TH, Gussekloo J, Koning F: Phenotypic and functional characterization of CD4 T cells expressing killer Ig-like receptors J Immunol 2004, 173:6719-6726 31 Warrington KJ, Kent PD,...

Ngày tải lên: 09/08/2014, 10:21

... enzymatical digestion of matrix components by chondroitinase ABC, pronase E, trypsin, collagenase P, or proteinase K) Finally, a digest with proteinase K (code S3004; Dako) for 15 minutes turned out ... carbon dioxide in serum-free DMEM/F12 Nutmix (DMEM/F12; Invitrogen, Karlsruhe, Germany) containing 0.1% pronase E (Sigma-Aldrich, Taufkirchen, Germany) in a spinner flask for fine mincing and digestion ... biotin in terms of a clearly lower background All antibodies were diluted in PBS containing 5% bovine serum albumin The signal was developed by incubation with hydrogen peroxide and DAB (diaminobenzidine...

Ngày tải lên: 12/08/2014, 15:23

... Stress-activated hypoxia-induced pathways were also shown to be important The PowerBlot showed increased expression of phosphorylated c-Jun N-terminal kinase/stress-activated protein kinase (JNK/SAPK) (activated ... protein expression of JAR cells induced by hypoxia in the apoptosis pathways using PowerBlot Protein Bad Bcl-x Caspase-3 Caspase-7 Hsp70-64kD Pan-JNK/SAPK1-50kD PTEN JNK-phospho-specific Pan-JNK/SAPK1-43kD ... Symonds E.M Increased placental apoptosis in intrauterine growth restriction Am J Obstet Gynecol 1997; 177 :1395-1401 Heikkilä A., Tuomisto T., Häkkinen S .K. , Keski-Nisula L., Heinonen S., Ylä-Herttuala...

Ngày tải lên: 31/10/2012, 15:12

... hepatocytes: role of protein kinase A Alcohol Clin Exp Res 1996;20:320A-24A 27 Lorenz K, Lohse MJ, Quitterer U Protein kinase C switches the Raf kinase inhibitor from Raf-1 to GRK-2 Nature 2003;426:574-9 ... C, Zhang H Kinetworks protein kinase multiblot analysis Methods Mol Biol 2003;218:99-111 25 Castaneda F, Kinne RKH Effects of doxorubicin, mitomycin C, and ethanol on Hep-G2 cells in vitro J ... ETS-domain transcription factor Elk-1 Biochem Soc Symp 2006:121-9 33 Faris M, Kokot N, Latinis K, et al The c-Jun N-terminal kinase cascade plays a role in stress-induced apoptosis in Jurkat cells...

Ngày tải lên: 31/10/2012, 15:37

... is mutated in a breast cancer cell line Nature 2001;413(6853):316-22 Yada M, Hatakeyama S, Kamura T, Nishiyama M, Tsunematsu R, Imaki H, Ishida N, Okumura F, Nakayama K, Nakayama KI Phosphorylation-dependent ... Results Mutational analysis of hCDC4 in AML In this study the common exons to 11 and the three known transcript variants of exon of hCDC4 (Figure 1A) were analyzed by direct DNA sequencing in 35 samples ... protein was found while screening 14 exons of 35 patient samples suggests that mutations of hCDC4 not play a significant role in the pathogenesis of AML During sequence analysis of exon 10 in the...

Ngày tải lên: 31/10/2012, 16:49

... regulation and localization of claudin expression It has been shown that protein kinases such as Protein Kinase A (PKA) and Protein Kinase C (PKC) can phosphorylate claudins (6,7) Phorbol ester treatment, ... 189-192 KTTS* [R /K] X[pS/pT] [R /K] XX[pS/pT] [R /K] [R /K] X[pS/ pT] KXX[pS/pT] 195-197 195-197 31-34 31-34 65-69 TPR TPR RIYS RIYS KVFDS [pS/pT]X[R /K] [pS/pT]X[R /K] RXXpS [R /K] XX[pS/pT] KXX[pS/pT] PKA PKC ... activating both PKA and PKC sites results in the cytoplasmic distribution of claudin-1 However, the deactivation of PKC sites does not cause nuclear localization of claudin-1 Unlike PKC, which...

Ngày tải lên: 03/11/2012, 11:17