directory and naming protocols
Directory and File Commands
Ngày tải lên :
02/10/2013, 09:20
... command to copy a directory and its contents to another directory If the directory does not exist, it is created by the cp command Command Format cp -r[i] source _directory( s) destination _directory ... program The format for the redirection of standard input, standard output, and standard error is: command > file or command >> file command < file command 2> file Angle Brackets ( >, ) Use ... data to a command Output – Receiving data from a command Error - Errors generated by a command Standard input Command Standard output The Linux computing environment enables command I/O to be...
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Tài liệu IP Multicast Distribution Trees and Control Protocols docx
Ngày tải lên :
11/12/2013, 14:15
... Distribution Trees and Control Protocols 2-23 Dense Mode protocols implement the “PUSH” model for multicast data delivery This model is also called broadcast and prune or flood and prune because ... mode and sparse mode intradomain protocols n Determine the current situation and protocols for interdomain multicasting Copyright © 2000, Cisco Systems, Inc IP Multicast Distribution Trees and ... Path Tree (SPT) between a source Source1 and receivers Receivers1 and Receiver2 We assume that the path between source and receivers over routers A, C and E is the path with the lowest cost Packets...
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Tài liệu Designing a Microsoft Windows Server 2003 Active Directory and Network Infrastructure docx
Ngày tải lên :
11/12/2013, 14:15
... access to Active Directory will be given to the help desk staff and the regional network administrators Although bandwidth is not currently an issue, and incremental increase in bandwidth usage ... high level of availability and reduced latency between the New York and Boston offices is required Bandwidth utilization between the Boston and New York offices is minimal and is not a concern in ... servers use little bandwidth on the slow ISDN lines QUESTION NO: You are designing the Active Directory infrastructure to meet the business and technical requirements You run ADSizer, and find that...
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Tài liệu How to cheat at installing, configuring and troubleshooting active directory and DNS doc
Ngày tải lên :
17/12/2013, 04:15
... for importing and exporting Active Directory objects, LDIFDE.EXE, which is found in the WINNT\SYSTEM32 directory LDIFDE stands for LDAP Directory Import File Directory Export, and uses the LDAP ... Active Directory Recovery Console Directory Service Restore mode is applicable only to Windows 2000 DCs for restoring the Active Directory service and SYSVOL directory Restore mode is a command-line ... rights reserved 21 DNS and Active Directory TOPIC 6: Design a Global Active Directory Domain and Trust Infrastructure There are four areas to document for an Active Directory and DNS namespace design...
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Tài liệu Networking and Desktop Protocols docx
Ngày tải lên :
21/12/2013, 19:15
... as broadcast frames, and excessive broadcasts reduce bandwidth for end users Later in this chapter, we’ll examine how to manage GNS requests and SAPs Networking and Desktop Protocols ○ ○ ○ ○ ○ ... the IPX model Answers a and b are incorrect, because EIGRP IPX and IPX are layer protocols Answer c is incorrect, because UPX is an invalid protocol Networking and Desktop Protocols 35 ○ ○ ○ ○ ... because it has no network layer addressing Answers a and b are incorrect, because IP and IPX are routable protocols Networking and Desktop Protocols 41 ○ ○ ○ ○ ○ ○ ○ ○ ○ ○ ○ ○ ○ ○ ○ ○ ○ ○ ○ ○...
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basic dna and rna protocols
Ngày tải lên :
10/04/2014, 11:03
... Longmire, J L , Albright, K L , Meincke, L J , and Hildebrand, C E (1987) A rapid and simple method for the isolation of high molecular weight cellular and chromosome-specific DNA m solutton without ... M and Hudson, A P (1989) Preparation of RNA from unspheroplasted yeast cells (Saccharomyces cerevwae) Anal Blochem 176,303-306 Isolation of RNA and DNA 20 Nemeth, G G., Heydemann, A., and Bolander, ... , and McGee, J D (1988) Simultaneous extraction from clinical biopsies of high-molecular-weight DNA and RNA: comparattve characterization by biotmylated and 32P-labeled probes on Southern and...
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basic protein and peptide protocols
Ngày tải lên :
10/04/2014, 11:03
... two alternative protein standards, bovine serum albumin and ovalbumin, are 0.66 and 0.75, respectively Plastic and glassware used in the assay should be absolutely clean and detergent-free Quartz ... polyacrylamide and starch gels, in Laboratory Techniques in Biochemistry and Molecular Biology, vol (Work, T S and Work, E., eds.), North Holland, Amsterdam, London, pp 1-149 Kistler, W S., Geroch, M E., and ... detergents and denaturing agents such as urea and guanidinium chloride, although it is more sensitive to the presence of reducing sugars Both a standard assay (0.1-1.0 mg protein/ml) and a microassay...
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antigen processing and presentation protocols
Ngày tải lên :
10/04/2014, 22:14
... SDS-PAGE analysis and proteasome activity assays, concentrate and store proteasomes according to Subheading 3.7 References Monaco, J J and Nandi, D (1995) Genetics of proteasomes and antigen processing ... 729–754 Coux, O., Tanaka, K., and Goldberg, A L (1996) Structure and functions of the 20S and 26S proteasomes Annu Rev Biochem 65, 801–847 Nandi, D., Marusina, K., and Monaco, J J (1998) How endogenous ... Cardozo, C., and Orlowski, M (1997) Bovine spleen multicatalytic proteinase complex (proteasome): replacement of X, Y, and Z subunits by LMP7, LPM2, and MECL1 and changes in properties and specificity...
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animal cell electroporation and electrofusion protocols
Ngày tải lên :
11/04/2014, 09:32
... commercial electroporation equipment, and many published and manufacturer-supplied protocols Many of these protocols are results of trial and error These empirical protocols are valuable guides for ... new and modified protocols are constantly neededto optimize the transfection yield Developing new protocols for new casesby trial and error in each laboratory is wasteful in terms of time and ... resistance R of the sample and any circuit elements The formulae relating voltage and capacitance to the relevant electric parameters E and T are sample- and Effects of Pulse Length and Strength 33 instrument-dependent...
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gene isolation and mapping protocols
Ngày tải lên :
11/04/2014, 09:42
... the alleles and at locus Dl The boy also carries alleles and at locus M15, whereas the girl carries alleles and The brown-haired boy carries alleles and at locus D 11, and alleles and at Ml 5, ... family, the parents are called and 2, the children are 3,4, and 5, and and are the paternal grandparents The next two columns contains the identification for the father and mother, respectively, of ... Dl and Ml All the grandparents are fortunately available and are homozygous for both markers Mrs Smith’s mother was red-haired and her father was brown-haired Mrs Smith, who is red-haired and...
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gene transfer and expression protocols
Ngày tải lên :
11/04/2014, 09:42
... CAUTION: Phenol is a strong oxidant and can cause severe burns Always wear gloves and exercise due care in handling Phenol is also light-sensitive Always prepare and store in an amber glass bottle ... upperband consists of open circular and linear molecules) using a 20-mL hypodermic syringe equipped with a 19gage needle Collect the banded material in a total vol of 4-5 mL 12 Either reband the ... Transfer and Expression Protocols Edited by: E J Murray 1991 The Humana Press Inc., Clifton, NJ 15 16 Okayama and Chen The calcium phosphate method was originally developed by Graham and van der...
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hemostasis and thrombosis protocols
Ngày tải lên :
11/04/2014, 09:45
... toxic and must be handled with great care Gloves, masks and safety glasses must be worn Phenol must be handled in a fume hood Ethidium bromide is a potent carcinogen and teratogen and must be handled ... Visualize and excise the band of interest under low power UV light and place into a sterile Eppendorf To denature the gel matrix and release the DNA, place the gel slice at –80°C for and then ... 139–148 12 Collen, D and Lijen, H R (1991) Basic and clinical aspects of fibrinolysis and thrombolysis Blood 78, 3114–3124 Isolation of DNA and RNA 25 Isolation of DNA and RNA David J Perry...
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hepatitis b and d protocols volume 1
Ngày tải lên :
11/04/2014, 09:45
... dCTP, and dGTP), 1.25 M each of primer and primer PCR mix 3–4: same as PCR mix 1–2, but use primer and primer instead of primer and primer PCR mix 5–6: same as PCR mix 1–2, but use primer and ... strand-specific primer probes (1561+ for −strand, 1590− for +strand) reveals positive strand specificity of the faster migrating DNA product due to asymmetric amplification (Fig 2A, positive strand ... Filled and open triangles point to the 370 and 235 bp amplification products (Middle and Bottom Panels) Hybridization to the primer probes 1561+ and 1752+ Table cDNA Plasmids Used as Competitors and...
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hepatitis b and d protocols volume 2
Ngày tải lên :
11/04/2014, 09:45
... Purification and characterization of duck lymphocytes and thrombocytes from peripheral From: Methods in Molecular Medicine, vol 96: Hepatitis B and D Protocols, volume Edited by: R K Hamatake and J ... 1980s to identify and separate duck lymphocytes into T and B cells (16) and to define conditions for the in vitro culture and optimization of responses to phytohemagglutinin (PHA) and concanavalin ... liver and spleen and methyl green for thymus and bursa, then dehydrate through AR ethanol and AR xylene and mount with DePeX 3.2.3 Identification of Kupffer Cells in the Liver and Phagocytic Cells...
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in vitro transcription and translation protocols
Ngày tải lên :
11/04/2014, 09:47
... mM dTTP, and mM mSdCTP 50X second-strand RX nucleotides: 7.5 mM dATP, 7.5 mM dGTP, 7.5 mA4 dTTP, and 35 mM dCTP 50X second-strand XR nucleotides: 7.5 n&f dATP, 7.5 mM dGTP, 7.5 mM dTTP, and 10 ... UTP The fulllength probes and protected fragmentsare 404 and 329 bp for IL-4 and 193 and 143 bp for P,-MG RNA was extracted from murine EL4 cells stimulated with PMA and ionomycin using the guanidinium ... nuclease- and protease-free if purchased racked) and sterile pipets The use of gloves is strongly advised to reduce nuclease and protease contaminations, and to uphold safety regulations for the handling...
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lipase and phospholipase protocols
Ngày tải lên :
11/04/2014, 09:49
... exposed to I2 vapor and lipid bands are visualized under UV light phosphatidylethanolamme, and lysophosphohpid 15 The plasmenylethanolamme, bands are identified, scraped, and quantified by phosphorus ... phosphoglyceroethanolamme band under UV light Scrape off the band and extract with mL of methanol 12 Read the fluorescence intensity at excttatlon and emlsslon of 340 and 376 nm, respectively Notes ... hydride and stir the mtxture at 0°C for 30 mm and then at room temperature for h Stop the reaction by coolmg the mrxture on Ice and adding water to the mtxture Extract the mixture wnh chloroform and...
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oxidative stress biomarkers and antioxidant protocols
Ngày tải lên :
11/04/2014, 10:00
... Biomarkers and Antioxidant Protocols METHODS IN MOLECULAR BIOLOGY TM John M Walker, Series Editor 215 Cytokines and Colony Stimulating Factors: Methods and Protocols, edited by Dieter Körholz and Wieland ... Methods and Protocols, edited by William C Copeland, 2002 196 Ultrastructural and Molecular Biology Protocols: edited by Donald Armstrong, 2002 195 Quantitative Trait Loci: Methods and Protocols, ... 199 Liposome Methods and Protocols, edited by Subhash C Basu and Manju Basu, 2002 198 Neural Stem Cells: Methods and Protocols, edited by Tanja Zigova, Juan R Sanchez-Ramos, and Paul R Sanberg,...
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plant cell electroporation and electrofusion protocols
Ngày tải lên :
11/04/2014, 10:10
... commercial electroporation equipment, and many published and manufacturer-supplied protocols Many of these protocols are results of trial and error These empirical protocols are valuable guides for ... new and modified protocols are constantly neededto optimize the transfection yield Developing new protocols for new casesby trial and error in each laboratory is wasteful in terms of time and ... resistance R of the sample and any circuit elements The formulae relating voltage and capacitance to the relevant electric parameters E and T are sample- and Effects of Pulse Length and Strength 33 instrument-dependent...
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plant gene transfer and expression protocols
Ngày tải lên :
11/04/2014, 10:10
... can be recovered on a 1.5-kb DraI fragment Unique EcoRI, WI, and KpnI sites and SmaI and BamHI sites are located respectively 5’ and 3’ of the promoter sequence in the gus fusion construct (63) ... Hildebrand, D F , and Hunt, A G (1987) Design and construction of a versatile system for the expression of’foreign genes m plants Gene 61, l-l Topfer; R., Matzeit, V., Gronenborn, B., Schell, J., and ... A., Kern, H F., and Scheele G A (1987) Selection of AUG initiation codons differs in plants and animals EMBO J 6,43-L48 Guerineau, F and Mullmeaux, P (1993) Plant transformation and expression...
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ribozymes and sirna protocols, 2nd ed
Ngày tải lên :
11/04/2014, 10:30
... 20, 3252 Chemical and Enzymatic RNA Synthesis Combination of Chemical and Enzymatic RNA Synthesis Rajesh K Gaur, Andreas Hanne, and Guido Krupp Summary The potential of standard in vitro transcription ... structure and activity (see Chapters 14 and 15) Therefore, appropriate vectors should be used In regard to siRNAs, U6, and H1 promoters were found to be the vector of choice (see Chapters 36, 38, and ... the homologous double-stranded promoter, the template types vary from the standard transcription plasmid to specifically designed PCR products and to mostly single-stranded templates, containing...
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