dig labeled crna probe for pv
BIODIVERSITY IN AGROECOSYSTEMS - CHAPTER 3 doc
Ngày tải lên :
11/08/2014, 17:20
... therefore, are forced to use existing pore spaces, cavities, or channels for locomotion within soil Habitable pore space (voids of sufficient size and connectivity to support mesofauna) accounts for ... food sources which are maintained even after the material has passed through the digestive tracts of other animals For example, the collembolans Proisotoma minuta and O encarpatus feed upon the ... (Vinciguerra, 1979) Microarthropods fragment detritus and increase surface area for further microbial attack (Berg and Pawluk, 1984) For example, collembolans and mites may enhance microbial activity, accelerate...
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EMPIRICAL STUDY ON EFFICIENCY AND SUSTAINABILITY OF SOIL PURIFICATION FACILITY FORDISCHARGED POLLUTANTS FROMROAD SURFACE
Ngày tải lên :
05/09/2013, 09:08
... values were about 60% for T-COD, 60% for T-P, 50% for T-N and 85% for SS before soil replacement The GRE values after soil replacement showed almost similar values as those before soil replacement, ... 95% and 20 – 95% for Tank A and Tank B, respectively Except for the first three initial surveys, the average GRE in Tank A were about 75% for T-COD and T-P, 65% for T-N and 90% for SS In tank B, ... '95 '05(Forecast) 14% 56.1 28% (t/day) 13% 29% 20% COD 34% 6% '05(Forecast) '95 21% 26% 44.7 21.6 (t/day) 15% 36% (t/day) 16% 5% 8% T-N 16% 27% 39% 16% 20.1 (t/day) T-P 16% 11% 13% '05(Forecast)...
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Tài liệu Báo cáo khoa học: Structure and function of active chromatin and DNase I hypersensitive sites pdf
Ngày tải lên :
14/02/2014, 18:20
... by increasing amounts of MNase digestion and probed directly with specific GM-CSF locus probes In this analysis chromatin fragments were prepared from T cells before or after stimulation of TCR ... oligo-nucleosome bands for bulk chromatin, but a smeared pattern for active chromatin An example of the phenomenon is presented in Fig 2A, which shows MNase digestion data for the human GM-CSF ... I thank Constanze Bonifer for her helpful advice and comments on the manuscript, and Karolin Luger and Jacques Cote for informative discussions I thank Graham Goodwin for giving me a good initiation...
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Tài liệu Báo cáo khoa học: Interaction between very-KIND Ras guanine exchange factor and microtubule-associated protein 2, and its role in dendrite growth – structure and function of the second kinase noncatalytic C-lobe domain docx
Ngày tải lên :
14/02/2014, 19:20
... v-KIND and the high-molecular-weight (HMW) form, but not the low-molecular-weight form, of microtubule-associated protein (MAP2) via 1652 KIND2 is critical for this signaling pathway [12] HMW-MAP2 ... core regions for the v-KIND–MAP2 interaction and showed that the MAP2 binding core is not only critical for targeting of v-KIND to neuronal dendrites, but also is indispensable for the function ... successfully detected a pull down for the combination of EGFP-KIND2 and GST-CD2-1-6-2, but not for other combinations (Fig S2C), suggesting that the core sequence critical for the specific v-KIND KIND2...
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Tài liệu Body Size: The Structure and Function of Aquatic Ecosystems pptx
Ngày tải lên :
17/02/2014, 19:20
... contributors for their efforts and remarkable efficiency, the British Ecological Society and the Freshwater Biological Association for their support, and the local organizers at the University of Hertfordshire ... central tendency) may not scale isometrically as expected for maximum prey size Unfortunately, information on predator–prey size relationships for suspension feeders is scarce and a general analysis ... interval Ackerman et al (2004) performed such an analysis for all of the fish coexisting at a site on the Great Barrier Reef, and found the predicted MÀ3/4 scaling – except for the smallest size classes,...
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Tài liệu Báo cáo khoa học: Expression and function of Noxo1c, an alternative splicing form of the NADPH oxidase organizer 1 doc
Ngày tải lên :
19/02/2014, 06:20
... PCR was performed using the following primers: ‘a’, 5¢-TCTCCCAAAGCTTCTCGATGC-3¢ (forward primer specific for the Noxo1 cDNA); ‘b’, 5¢-CCCAAAGCTTCTCGGTCAGGC-3¢ (forward primer specific for the Noxo1c ... cotransformed into competent yeast HF7c cells containing a HIS3 reporter gene, as previously described [15] After the selection for Trp+ and Leu+ phenotype, the transformants were tested for their ... AATCCCATCACCATCTTCCA-3¢ (forward primer) and 5¢-CCTTCTCCATGGTGGTGAAGAC-3¢ (reverse primer) were used for the glyceraldehyde-3-phosphate dehydrogenase cDNA PCR analyses were performed using ABI PRISMÒ...
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Tài liệu Báo cáo khoa học: Structure and function of plant aspartic proteinases pptx
Ngày tải lên :
19/02/2014, 12:20
... mechanism has also been proposed for cenprosin, the AP from Centaurea calcitrapa [30] and for recombinant oryzasin 1, the rice AP [29] A slightly different picture has emerged for prophytepsin Using metabolic ... process the PSI is totally removed, probably by an aspartic proteinase, before the prosegment Its conversion into an active form is likely to occur inside the vacuoles where the protein is accumulated ... prophytepsin, the precursor form of barley AP containing the prosegment and the PSI (PDB code: 1QDM) [25] (Fig 2) Both APs are two-chain polypeptides in their mature forms and present a very similar...
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Tài liệu Báo cáo khoa học: a-Conotoxins as tools for the elucidation of structure and function of neuronal nicotinic acetylcholine receptor subtypes doc
Ngày tải lên :
19/02/2014, 12:20
... target for prey capture However, the genetic information for ÔunderdevelopedÕ a-conotoxins targeting other nAChR subtypes might still be present in the snails and could supply novel ligands for ... Deutsche Forschungsgemeinschaft (NI 592/2-1) We thank Heinrich ganglia neurons and bovine chromaffin cells and assumed to Betz for his support and for critical reading of the manuscript be selective for ... selectivity for different nAChR subtypes, with all known a3/5-conotoxins being selective for the muscle-type nAChR, while the only published a4/6-conotoxin and most a4/7-conotoxins are selective for...
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Tài liệu Báo cáo khoa học: Cold survival in freeze-intolerant insects The structure and function of b-helical antifreeze proteins pdf
Ngày tải lên :
19/02/2014, 16:20
... comparison of isoform sequences can demonstrate which residues are important for a protein’s function and structure A list of known isoforms may be found in Doucet et al [55] for sbwAFP and in ... parallel b-sheets of the protein are designated PB1, PB2 or PB3 for sbwAFP For TmAFP, there is only one face of the protein that forms a parallel b-sheet, with the strand of the coil indicated ... for sbwAFP and in Liou et al [36] for TmAFP Given the highly repetitive structure of the b-helices, one would expect repetitive sequences For TmAFP, the isoforms shows a 12-residue consensus...
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Tài liệu Báo cáo khoa học: "Beefmoves: Dissemination, Diversity, and Dynamics of English Borrowings in a German" ppt
Ngày tải lên :
19/02/2014, 19:20
... Baseline n-gram classifier accuracy for n= 94.80 97.74 99.35 99.85 99.96 99.98 Figure 1: Accuracy of the baseline classifer on word lists; 10-fold CV; std deviations ≤ 0.02 for all cases Affix no no yes ... stem flow takes German participial affixes We therefore included a template-based affix-stripping preprocessing step, removing common German affixes before feature extraction Because of the possibility ... entire MZEE corpus, this study uses the 851 most common anglicism word forms identified by our system, treating all word forms as distinct We split the MZEE corpus into six non-overlapping windows...
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Tài liệu Báo cáo Y học: Structural diversity and transcription of class III peroxidases from Arabidopsis thaliana docx
Ngày tải lên :
21/02/2014, 01:21
... sequencing were performed as described previously [19] and both strands were sequenced Genes encoding class III peroxidases in Arabidopsis were searched for in the Munich Information Center for Protein ... analyses were performed twice for each peroxidase using two different reverse transcribed reactions for each time point and organ As a control of the quality of the mRNA, RT-PCR was performed with ... electrophoresis The predicted sizes of the amplification products are 560 bp for AtP16, 420 bp for AtP13, 574 bp for AtP22, and 474 bp for ef-1a The larger size products represent amplification of traces...
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Báo cáo khoa học: Proteolytic activation and function of the cytokine Spatzle in the innate immune response of a lepidopteran insect, Manduca sexta ppt
Ngày tải lên :
06/03/2014, 09:22
... proSpatzle-1, with no evidence for a ¨ longer form Ten splicing isoforms of Drosophila Spat¨ zle occur in the precellular blastoderm embryo [8] One pair of splicing isoforms appears as D melanogaster ... active form ¨ (C106) [7,11,17], and to Te molitor SPE [24] led us to predict that HP8 is an activating proteinase for M sexta proSpatzle [31] To test this hypothesis, we ¨ prepared a recombinant form ... synthesized as described previously [31] Each cDNA sample (diluted : 500 for hemocyte cDNA or : 25 for fat body cDNA) was used as template for quantitative RT-PCR analysis The M sexta ribosomal protein...
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Báo cáo khoa học: NMR solution structure and function of the C-terminal domain of eukaryotic class 1 polypeptide chain release factor pdf
Ngày tải lên :
06/03/2014, 11:20
... Mantsyzov et al Table Statistics for the two ensembles of the calculated structures of the human C-domain (24 structures for the open conformer and 24 for the closed conformer were analyzed) Open Closed ... protein conformers (D, E) The conformations of the minidomain in the open (D) and closed (E) protein conformers The residues participating in key interactions that could stabilize the two conformers ... chain could be crucial for protein folding and for the stabilization of the conformers At pH values above its pKa, the imidazole ring of His is uncharged, and both conformers are stable In attempts...
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Báo cáo khoa học: Evolutionary changes to transthyretin: structure and function of a transthyretin-like ancestral protein doc
Ngày tải lên :
07/03/2014, 00:20
... performed RNA interference (RNAi) studies in C elegans to determine a loss-of-function phenotype for R09H10.3 and ZK697.8 TLP genes RNAi-treated worms were scored for embryonic lethality and for ... a possible phenotype was not performed For example, the worms were not subjected to any type of environmental stress In addition, RNAi was performed using dsRNA for a single TLP gene at a time ... in C elegans may have been more informative had double-knockdown studies been performed A role for TLP in purine metabolism was first proposed in 2001 In an effort to develop a greater understanding...
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Báo cáo khoa học: Synthesis and function of ribosomal proteins – fading models and new perspectives pptx
Ngày tải lên :
07/03/2014, 01:20
... ribosome is essential for the cell Accordingly, most of the analyzed eukaryotic RPs have been reported as being essential for growth [9,10] It can be postulated that RPs are required for different steps ... explanation for the inconsistencies could be that additional factors contribute to the regulation For instance, Ro60 is known to interact with La and CNBP ⁄ ZNF9, whereas small RNAs (Y) form a complex ... phosphorylation was necessary for the recruitment of TOP messengers to the polysomes [38] For years, RPS6 has been considered to be the key protein responsible for the selective translation of...
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Báo cáo khoa học: Structure and function of KH domains docx
Ngày tải lên :
07/03/2014, 05:20
... which may be a consequence of stability requirements for selection in vitro [37] The stem of the hairpin adopts the A-form doublehelical conformation, with four Watson–Crick base pairs (G1–C20, ... P-element somatic inhibitor protein (PSI), for example, bind ligand cooperatively [25] The KH1–KH2 domains of NusA (Protein Data Bank entries 2ATW and 2ASB) form an uninterrupted recognition surface ... interactions with an N-terminal helix known as the QUA2 domain (labeled in Fig 7) [36] The RNA adopts an extended single-stranded conformation, and The X-ray structure of the KH3 domain of Nova-2...
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![Báo cáo khóa học: Selective release and function of one of the two FMN groups in the cytoplasmic NAD + -reducing [NiFe]-hydrogenase from Ralstonia eutropha pptx](https://media.store123doc.com/images/document/14/rc/gg/medium_ggg1394180403.jpg)
Báo cáo khóa học: Selective release and function of one of the two FMN groups in the cytoplasmic NAD + -reducing [NiFe]-hydrogenase from Ralstonia eutropha pptx
Ngày tải lên :
07/03/2014, 15:20
... dialysis bag was aerated for and then assayed for FMN The solution inside the dialysis bag was tested for NADH-K3Fe(CN)6 activity and acid-labile FMN The experiment has been performed with three different ... Amsterdam) is acknowledged for the use of the Spex Fluorolog III fluorimeter This work was supported by the Netherlands Organization for Scientific Research (NWO), the Deutsche Forschungsgemeinschaft, ... R.M.C., Elliot, D.C., Elliot, W.H & Jones, K.M (1986) Data for Biochemical Research Oxford Science Publications, Clarendon Press, Oxford, UK Axley, M.J., Keefe, R.G., Falk, M.C & Harabin, A.L...
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Báo cáo khoa học: Structure and function of N-acetylglucosamine kinase Identification of two active site cysteines pptx
Ngày tải lên :
08/03/2014, 10:20
... increased Km for GlcNAc, whereas the Km for ATP was increased only 2.5-fold The opposite was found for C143S, where the respective increases in Km were fivefold for GlcNAc and 10-fold for ATP Both ... sixfold increase in the Km for GlcNAc, whereas the Km for ATP was unchanged This suggests that C217 may have a role in GlcNAc binding For C211 and C268 the Km values for both substrates were increased, ... of specific cysteine residues of GlcNAc kinase, the Km values for both substrates, GlcNAc and ATP were determined for all mutants and for the wild-type enzyme (Table 2) In Table Kinetic data of...
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Báo cáo Y học: Structure, mechanism and function of prenyltransferases docx
Ngày tải lên :
08/03/2014, 22:20
... chains [50,57] Lipid carrier for peptidoglycan synthesis Lipid carrier for glycoprotein synthesis Farnesylated Ras for signal transduction Geranylgeranylated Rab for signal transduction Precursor ... helix has been suggested to serve as a hinge for the interconversion of two conformers This study also suggested a role for a Trp residue in the loop for FPP binding and catalysis [69] Fluorescent ... UPPS conformation to a closed form results in better interaction between the enzyme and the substrates and intermediates After the reaction, the UPPS structure shifts to an open form for product...
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Báo cáo khóa học: S-(2,3-Dichlorotriazinyl)glutathione A new affinity label for probing the structure and function of glutathione transferases potx
Ngày tải lên :
16/03/2014, 16:20
... filtration on Sephadex G-25 The pH used for inactivation (pH 6.5) was the same as that necessary for high GST activity This probably affords an enzyme conformation similar to that adopted during ... drugs for protecting normal cells from chemotherapeutics [28] Recently, GST I has been successfully applied as an analytical enzyme for the determination of herbicides in solution [29] Therefore, ... lM, for the fast and slow reactions, respectively, were estimated Apparent maximal rate constants were determined to be 0.049 ± 0.002 min)1 for the fast reaction, and 0.0153 ± 0.001 min)1 for...
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