differentiation of dendritic cells from human embryonic stem cells

... derivation of human embryonic stem cells Stem cells 2005, 23:1228-1233 Moon SY, Park YB, Kim D-S, Oh SK, Kim D-W: Generation, culture, and differentiation of human embryonic stem cells for therapeutic ... hematopoietic differentiation ofhuman embryonic stem cells Blood 2003, 102:906-915 Wang L, Li L, Menendez P, Cerdan C, Bhatia M: Human embryonic stem cells maintained in the absence of mouse embryonic ... Therapeutic potential of embryonic stem cells Blood Rev 2005, 19:321-331 Menendez P, Wang L, Bhatia M: Genetic manipulation of human embryonic stem cells: A system to study early human development...

Ngày tải lên: 13/08/2014, 09:21

... temporary relief of symptoms Stem cells represent a promising cell source for cartilage repair and can be derived from two major sources: mesenchymal stem cells (MSCs) and embryonic stem cells (ESCs) ... SYSTEM…………………………………………… 65 4.1.1 Pluripotency of human embryonic stem cells …………………… 65 4.1.2 Effects of culture conditions in modulation of chondrogenesis…….66 4.1.3 Effects of culture conditions on hypertrophic ... chondrogenic cells Fig 22 Tumorigenicity of hESC-derived chondrogenic cells Fig 23 ECM modulation of post-expansion differentiation capability of hESC-derived chondrogenic cells Fig 24 Response of hESC-derived...

Ngày tải lên: 11/09/2015, 09:57

... Characterization of the transplanted vascular cells derived from human ES cells (HES-3) Characterization of the transplanted vascular cells derived from human ES cells (HES-3) A, Flow cytometric analysis of ... VE-cadherin (+) cells VE-cadherin (-) aSMA (+) cells hES -ECs hES -ECs+MCs aSMA (+) cells hES -MCs Figure cells differentiated from human of the vascular Schematic representation of preparationES cellstransplanted ... Preparation of human ECs and/or MCs derived from human ES cells Maintenance of human ES cell line (HES-3) was described previously [10] We plated small human ES colonies on OP9 feeder layer to induce differentiation...

Ngày tải lên: 18/06/2014, 15:20

... pluripotent stem cells However, less is known about the conservation and variability of DNA methylation across different stem cell lines A recent analysis of about 1% of the genome of HESC lines ... Feinberg AP: Differential methylation of tissue- and cancer-specific CpG island shores distinguishes human induced pluripotent stem cells, embryonic stem cells and fibroblasts Nat Genet 2009, ... changes of methylation levels between pairs of cell lines at binding sites with the changes of the expression levels of their associated genes The coordinates of TFBSs were downloaded from Motifmap...

Ngày tải lên: 09/08/2014, 23:20

... expression pattern of undifferentiated and differentiated human embryonic stem cells Stem Cells Dev 2007, 16(6):1003-1016 Page 15 of 17 (page number not for citation purposes) Journal of Translational ... 13 The expression of miR-92 has been reported in human embryonic stem (ES) cells [16,26], mouse ES cells[ 20] or Page 12 of 17 (page number not for citation purposes) Journal of Translational Medicine ... adult cells were Human Microvascular Endothelial Cells (HMVEC), Human Umbilical Vein Endothelial Cells (HUVEC), Umbilical Artery Smooth Muscle Cells (UASMC), Normal human astrocytes (NHA) (all from...

Ngày tải lên: 18/06/2014, 15:20

... culture of ICM cells with stem cell-like morphology from human blastocysts (Fig 1.1A) though cultures failed beyond passages The first long-term culture (4-5 months) of human embryonic stem cells ... stages of embryonic development from fertilization to E6.5 are represented in this image Embryonic Stem (ES) cells are derived from the inner cell mass while epiblast stem cells (EpiSCs) are of epiblast ... μ – Mu ° – Degree x CHAPTER 1: GENERAL INTRODUCTION 1.1 Embryonic stem cells The isolation of mouse and human embryonic stem cells (ES cells) heralded a new era in regenerative medicine, raising...

Ngày tải lên: 04/10/2015, 16:03

... VS, Jones JM Embryonic stem cell lines derived from human blastocysts Science 1998;282(5391):1145-7 Reubinoff BE, Pera MF, Fong CY, Trounson A, Bongso A Embryonic stem cell lines from human blastocysts: ... Methods 1983; 65: 55-63 Heng BC, Liu H, Rufaihah AJ, Cao T Human embryonic stem cell (HESC) colonies display a higher degree of spontaneous differentiation when passaged at lower densities In Vitro ... bioreactors Conflicts of interest The authors have declared that no conflict of interest exists References Becker EW The roles of ATP in the dynamics of the actin filaments of the cytoskeleton...

Ngày tải lên: 31/10/2012, 16:57

... enrichment of cardiomyocytes derived from human embryonic stem cells Circ Res 2002, 91:501-508 He JQ, Ma Y, Lee Y, Thomson JA, Kamp TJ: Human embryonic stem cells develop into multiple types of cardiac ... Derivation of DCs from hES cells Derivation of DCs from hES cells: Undifferentiated hES cells were cocultured with S17 mouse stromal cells to derive cystic bodies Later, purified CD34+ cells derived from ... derivation of macrophagres from hES-CD34+ cells whereas studies of Galic et al [20] derived functional T cells from hES-CD34+ cells in vivo using humanized mice Whether the hES derived T cells support...

Ngày tải lên: 10/08/2014, 05:20

... epigenomic profiling of a cohort of human embryonic stem cell (hESC) lines can be used to generate hESC reference epigenomes (left panels) The extent of reprogramming and differentiation potential of individual ... embryonic stem cell; H3K4me1, methylation of lysine of histone H3; H3K4me3, trimethylation of lysine of histone H3; H3K27ac, acetylation of lysine 27 of histone H3; H3K27me3, trimethylation of ... stem cell; hESC, human embryonic stem cell; H3K4me3, trimethylation of lysine of histone H3; H3K27ac, acetylation of lysine 27 of histone H3; H3K27me3, trimethylation of lysine 27 of histone H3;...

Ngày tải lên: 11/08/2014, 12:21

... colony-forming cells derived from human embryonic stem cells Proc Natl Acad Sci USA 2001, 98:10716-10721 Lu S-J, Li F, Vida L, Honig GR: CD34+CD38- hematopoietic precursors derived from human embryonic stem ... promote hematopoietic differentiation of human embryonic stem cells Blood 2003, 102:906-915 Woll PS, Martin CH, Miller JS, Kaufman DS: Human embryonic stem cell-derived NK cells acquire functional ... development from human embryonic stem cell lines Exp Hematol 2005, 33:987-996 Zambidis ET, Peault B, Park TS, Bunz F, Civin CI: Hematopoietic differentiation of human embryonic stem cells progresses...

Ngày tải lên: 14/08/2014, 08:20

... characterization of lncRNAs involved in differentiation has been carried out in human cells In this thesis, functional characterization of lncRNAs in human development is addressed using human embryonic stem ... process of regional specification of midbrain by addition of the right concentrations of SHH and FGF8, several groups reported the efficient generation of dopamine neurons from embryonic stem cells ... Maintenance of ReN-VM cells Differentiation of ReN-VM cells 32 32 3.7 Small-interfering RNA (siRNA)-mediated gene silencing 33 3.7.1 3.7.2 Transfection of H1 hESCs with siRNAs Transfection of ReN-VM cells...

Ngày tải lên: 09/09/2015, 17:54

Ngày tải lên: 09/09/2015, 17:54

... Schematic representation of differentiation of ReN-VM neural stem cells following transfection of siRNAs Neural stem cells were plated one day prior to siRNA transfection Neuronal differentiation was ... resulted in loss of OCT4 The percentage of OCT4+ cells from Figure 6.7 was quantified by flow cytometry The number of OCT4+ cells were significantly reduced upon knockdown of any of the three pluripotent ... profiles of these lncRNAs mirror that of known drivers of neuronal differentiation (Figures 5.2E-F), and were therefore indicative of important roles of the lncRNAs in neurogenesis A group of...

Ngày tải lên: 09/09/2015, 17:54

... 8.4) In humans, RMST is located on chromosome 12q23, and the ENCODE ChIP-seq data from human embryonic stem cells (H1) and neuroblastomas (PFSK-1) indicated a REST binding peak upstream of RMST, ... down the neurogenic program of neural stem cells, with the cells adopting a glia lineage instead (Figure 7.6) On the other hand, overexpression of RMST enhanced neuronal differentiation efficiency ... significantly fewer TUJ1+ cells, indicating a loss of neurogenesis The scale bar indicates 100 µm   132  Figure 8.8: Overexpression of RMST enhanced neuronal differentiation of ReNVM cells (A) RMST was...

Ngày tải lên: 09/09/2015, 17:54

... Induction of pluripotent stem cells from adult human fibroblasts by defined factors Cell 131, 861-872 Takahashi, K., and Yamanaka, S (2006) Induction of pluripotent stem cells from mouse embryonic ... E.R (2009) Isolation and characterization of neural crest stem cells derived from in vitro-differentiated human embryonic stem cells Stem Cells Dev 18, 1059-1070 Johnson, R., Teh, C.H., Jia, H., ... T., Tabar, V., and Studer, L (2007) Isolation and directed differentiation of neural crest stem cells derived from human embryonic stem cells Nat Biotechnol 25, 1468-1475 Lee, S.M., Danielian,...

Ngày tải lên: 09/09/2015, 17:55

... mechanisms of regulation of NSC differentiation 1.1.5.2 Pluripotent stem cell-derived NSCs Human embryonic stem cells (hESCs), which are pluripotent cells derived from the inner cell mass of blastocyst-stage ... HUMAN EMBRYONIC STEM CELL-DERIVED NEURAL STEM CELLS: DERIVATION, DIFFERENTIATION AND MICRORNA REGULATION KWANG WEI XIN TIMOTHY (B.Sc (Hons), NUS) A THESIS SUBMITTED FOR THE DEGREE OF DOCTOR OF ... localization of mitotically active cells near the surface of ventricles and not in the overlying cortical layers of the embryonic human brain, and formulated the concept that all neurons were derived from...

Ngày tải lên: 10/09/2015, 09:08

... Characterization of stem cells by derivation source Category Embryonic stem cells Embryonic germ cells Fetal stem cells Umbilical cord stem cells Umbilical cord blood stem cells Umbilical cord matrix stem cells ... sources, named embryonic stem cells Literature review (ESCs), embryonic germ cells, fetal stem cells, umbilical cord stem cells, adult stem cells and induced pluripotent stem (iPS) cells (Ariff ... for schematic diagram of derivation of hESC lines from a human blastocyst Figure Schematic diagram of derivation of hESC lines from a human blastocyst (Adopted from NIH stem cells report 2001) 1.1.2...

Ngày tải lên: 11/09/2015, 10:00

... 2.7 Embryonic stem cells and induced pluripotent stem cells Embryonic stem cells (ESC) have two characteristics that distinguish them from other stem cells, in that they are capable of long-term ... understanding of the role of SHH in humans, that is based largely on the mouse model Figure 2.6 Human embryonic stem cells (hESC) derived from the blastocyst are able to differentiate into cells from ... used for daily hESC culture feeding 3.2.3 Human embryonic stem cells and induced pluripotent stem cells Human embryonic stem cell line HES-3 (46, XX) was from ES Cell International (Singapore, http://www.escellinternational.com)...

Ngày tải lên: 11/09/2015, 10:14

... specific diseases in the course of ex vivo gene therapy 1.1.1 Human Embryonic Stem Cells Human embryonic stem (hES) cells are cells derived from the inner cell mass of human blastocysts The hES cell ... achieve desirable level of transient transgene expression in human embryonic stem cells Efficient gene transfers to human embryonic stem cells were obtained after optimization of the promoters and ... MODIFICATION OF HUMAN EMBRYONIC STEM CELLS DU JUAN (B Sc.) A THESIS SUBMITTED FOR THE DEGREE OF DOCTOR OF PHILOSOPHY DEPARTMENT OF BIOLOGICAL SCIENCES NATIONAL UNIVERSITY OF SINGAPORE AND INSTITUTE OF...

Ngày tải lên: 11/09/2015, 14:38

... Derivation of human EG cells from aborted fetus has also been reported (Shamblott, Axelman et al 1998) The third type of stem cell is the adult stem cells Like ES and EG cells, adult stem cells can ... Generation of Hepatocyte-like cells from Mouse Embryonic Stem (ES) and Bone Marrow (BM) Cells YIN YIJUN A THESIS SUBMITTED FOR THE DEGREE OF DOCTOR OF PHILOSOPHY OF MEDICINE DEPARTMENT OF OBSTETRICS ... Itskovitz-Eldor et al 1998; Reubinoff, Pera et al 2000) The second type of stem cell is embryonic germ (EG) cells isolated from the primordial germ cells of the gonadal ridge of the fetus (Matsui, Zsebo...

Ngày tải lên: 16/09/2015, 12:10