dhea antiviral properties in cell cultures and animal models
Tài liệu Báo cáo khoa học: A novel splice variant of occludin deleted in exon 9 and its role in cell apoptosis and invasion docx
Ngày tải lên :
18/02/2014, 18:20
... that the intracellular calcium level increased in OccWT-overexpressing cells, and that 2-APB inhibited the effects of OccWT Therefore, the increase in intracellular calcium may explain the induction ... exon and exon 1a, we compared the promoters involved in Chang and Huh7 cells As shown in Fig 1D, PCR products containing exon were produced in Huh7 cells but not in Chang cells To determine whether ... of occludin in apoptosis and invasion J.-M Gu et al A B C D E F Fig Occludin increases the intracellular calcium concentration in Chang cells (A) Representative cell images obtained using a confocal...
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Báo cáo khoa học: Molecular and functional characterization of a novel splice variant of ANKHD1 that lacks the KH domain and its role in cell survival and apoptosis docx
Ngày tải lên :
07/03/2014, 21:20
... functions including apoptosis in infected and uninfected bystander target cells through its interaction with host cellular proteins [22–25] However, it is not clear at this point how interaction ... signaling, independent of MAP kinase (MAPK) by either functioning downstream of MAPK or by defining a new pathway of RTK signaling [8] RTKs play important roles in cell signaling during cell proliferation, ... synthesis in tested cell lines and this siRNA was used in subsequent assays Following transfection of VBARP siRNA into HeLa and NT2 cells, physical observation revealed that cell death occurred in a...
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báo cáo khoa học: " Manufacture of IRDye800CW-coupled Fe3O4 nanoparticles and their applications in cell labeling and in vivo imaging" ppt
Ngày tải lên :
11/08/2014, 00:22
... applied to in vitro cell labeling and in vivo imaging Methods Cells, animals and chemicals The RAW264.7 cell line was purchased from the China Center for Type Culture Collection, Chinese Academy ... Odyssey Infrared Imaging System Cell labeling with IRDy800CW-MNPs To investigate the labeling of cells with nanoparticles, RAW264.7 cells were seeded into cell culture plates and cultured in DMEM ... Biosciences), which absorb and emit in higher wavelength light (ex/em, 774/789 nm), and investigated their applicability in cell labeling and in vivo imaging Results and discussions Preparation...
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Báo cáo toán học: " Exchange Symmetries in Motzkin Path and Bargraph models of Copolymer Adsorption" ppsx
Ngày tải lên :
07/08/2014, 06:23
... main diagonal In both models of bargraphs and Motzkin paths, a two dimensional lattice model of an adsorbing polymer can be defined by letting the path be attracted to an adsorbing line In models ... analyse, and in this paper we only succeeded in solving a model where edges interact with the adsorbing line We shall also briefly consider the model with vertices interacting with adsorbing line; ... techniques outlined in the proof of Theorem 5.2 in Chapter of Flajolet and Sedgewick [10] We must evaluate the integral in equation (35) Substitute z = (1 + s/n)/3, and expand the resulting integrand asymptotically...
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Báo cáo sinh học: "Inequality of maximum a posteriori estimators with equivalent sire and animal models for threshold traits" pps
Ngày tải lên :
09/08/2014, 18:22
... for point and interval estimation of breeding values for threshold traits In: l!5th Annual Meeting of the European Association for Animal Production Edinburgh, 5-8 September 1994 Meijering A, ... differences in efficiency between QBLUP and MAPE only when the incidence was high (> 90 %, binary response) and with high (> 0.20) heritability (Meijering and Gianola, 1985; H6schele, 1988) When applying ... of MAPE and the proportion of selected candidates was not found to be of a simple kind (Mayer, 1991) Somewhat in contrast to these findings in the simulation studies were the results of investigations...
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Báo cáo y học: " Single cell studies of the cell cycle and some models" pps
Ngày tải lên :
13/08/2014, 22:22
... is still cell- to -cell variation in cycle stage and this can obscure the fine detail of the cycle Single cell measurements may help here Single cell analysis in yeast Returning to single cell analyses ... distinction between single cells and synchronous cultures does of course depend on the frequency and accuracy of the data points If the points have too much scatter, the fine detail of the single ... synchronous cultures in hiding the fine detail of increases in volume or area could well mean that single cell studies might reveal more than an exponential increase There might even be something like...
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Báo cáo lâm nghiệp: "Changes in phenolic acids and stilbenes induced in embryogenic cell cultures of Norway spruce by two fractions of Sirococcus strobilinus mycelia" pot
Ngày tải lên :
07/08/2014, 10:21
... decrease in isorhapontin (occurring in the highest concentrations in embryogenic cell cultures) , astringin and piceid levels was observed in WF-elicited cells after 12 h, while in PF-treated cells ... changes in (soluble and cell wall-bound) phenolic and stilbene concentrations during defence response in Norway spruce embryogenic cell suspension cultures induced by intracellular protein and wall ... (iRHAP), astringin (ASTR) and piceid (PIC), determined in Norway spruce cell cultures treated with 5% and 20% A abietina culture filtrate and from the control cells (C) Means of two independent...
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báo cáo khoa học: " Salivary a-amylase exhibits antiproliferative effects in primary cell cultures of rat mammary epithelial cells and human breast cancer cells" docx
Ngày tải lên :
10/08/2014, 10:21
... trypsin For cell counting and passaging, trypsin/EDTA (0.15%) was used to detach cells, and its reaction was stopped with fetal calf serum (20%) in DMEM/F12 Remaining passage (P0)-cells were allowed ... of cells, exclusively viable, and for dead cells after and 50 U/ml for day, days, and days (n = 4-14 wells per group) For counting, cells were detached with trypsin/EDTA, and viable and dead cells ... showing distinct sensitivity Thus, the impact of a-amylase on cell growth in vitro depends on cellular conditions, origin, e.g rat strain, and distinct cellular characteristics The rat primary cells...
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báo cáo khoa học: " Quantitative 1H NMR metabolomics reveals extensive metabolic reprogramming of primary and secondary metabolism in elicitor-treated opium poppy cell cultures" pptx
Ngày tải lên :
12/08/2014, 05:20
... 2-oxoglutarate, choline, and amino acids, such as leucine, valine, isoleucine, tyrosine and asparagine were found at higher levels in control extracts at 80 and 100 h, and discriminated http://www.biomedcentral.com/1471-2229/8/5 ... detected in control and elicitortreated samples The levels of most amino acids increased between 50 and 100 h in control cultures, but remained low in elicitor-treated cells The amino acids glutamine ... oldest medicinal plant and produces several pharmaceutically important benzylisoquinoline alkaloids, including the analgesics morphine and codeine, the muscle relaxant and vasodilator papaverine, the...
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Báo cáo y học: "Antiviral therapy of HCV in the cirrhotic and transplant candidate"
Ngày tải lên :
31/10/2012, 17:11
... more aggressive dosing of interferon and ribavirin [13, 14] Finally, growing experience with antiviral therapy in this high-risk group has led to novel approaches and increasing success Table ... unmodified interferon or peginterferon at low initial dose, with increases every two weeks toward standard target doses Duration of therapy was intended to be 24 weeks in genotypes and and 48 weeks in ... G-CSF to maintain ANC > 1500 cells/mL was well tolerated, with temporary dose discontinuation in 3/20 (15%) Forns and colleagues used relatively high-dose interferon/ribavirin therapy in their...
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The Retinoblastoma Gene Family in Cell Cycle Regulation and Suppression of Tumorigenesis
Ngày tải lên :
25/10/2013, 21:20
... containing the DNA binding motif but not the pRb-binding or transactivation domain or introduction of a competitor plasmid containing multiple E2F binding sites, preventing the binding of E2F and ... and cell cycle control Cell 81:323–330 Welch PJ, Wang JY (1993) A C-terminal protein-binding domain in the retinoblastoma protein regulates nuclear c-Abl tyrosine kinase in the cell cycle Cell ... while increased apoptosis was only observed in follicular cells lacking both pRb and p107 (Ruiz et al 2004) Interesting observations were made in cells lacking pRb in the retina Somatic inactivation...
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Tài liệu Báo cáo " Effecting of medium composition on biomass and ginsenoside production in cell suspension culture of Panax vietnamensis Ha et Grushv " doc
Ngày tải lên :
12/02/2014, 10:20
... biomass and ginsenoside yield of Ngoc Linh ginseng cell culture Materials and methods Induction of callus Fresh mountain ginseng roots were collected from Ngoc Linh mountain, Quang Nam province ... containing 3% sucrose Effects of application sole nitrate (NO3-) and in combination with ammonium (NH4+) on production of ginseng saponin and polysaccharides by suspension cultures of Panax ginseng ... through callus induction from the cultivated plant root [5] The cell line was maintained in MS liquid medium supplemented with mg/L indole-3-butyric acid (IBA), 0.1 mg/L of kinetin and 30 g/L sucrose...
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Tài liệu Báo cáo khoa học: Inhibitory properties of cystatin F and its localization in U937 promonocyte cells ppt
Ngày tải lên :
19/02/2014, 17:20
... prominent in early and late endosomes [37] Our results suggest that in U937 cells cathepsins H and X concentrate in different vesicles from those containing cathepsins B, C, F, K and L, and that ... Cystatin F in U937 cells lower inhibition constants for cathepsins S and H was due mainly to the low kass values Colocalization of cystatin F and potential target enzymes in U937 cells Using confocal ... [s)1] 10)6 ã kass [M)1ặs)1] Cathepsin F Cathepsin K Cathepsin V Cathepsin S Cathepsin H Cathepsin C Cathepsin X Papain Cathepsin La Legumainb Cathepsin Ba Papaina 0.17 0.05 0.35 0.15 0.30 0.15...
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Tài liệu Báo cáo khoa học: Functional hierarchy of plasminogen kringles 1 and 4 in fibrinolysis and plasmin-induced cell detachment and apoptosis docx
Ngày tải lên :
20/02/2014, 01:20
... formation, to a decrease in plasmin binding or to a combination of the two We demonstrate that both mAb 34D3 and mAb A10.2 inhibit plasmin binding to cells (Fig 5A) and fibrin (Fig 5B) in a concentration-dependent ... tion into plasmin in situ However, data linking plasminogen conformations and LBS accessibility to its functional properties such as binding to fibrin and cells, activation on these surfaces and ... thus indicating accessibility and participation of both K1 and K4 in firmly anchoring plasmin to fibrin and cell surfaces Because the expanded Lys-form of plasminogen may be an intermediary in plasmin...
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Signaling at the Cell Surface in the Circulatory and Ventilatory Systems docx
Ngày tải lên :
05/03/2014, 22:21
... During integrin outside in signaling, the binding of integrins to their extracellular ligands changes the integrin conformation and promotes integrin clustering The unidirectional signalings, in ... Signaling calmodulin and troponin-C contain calcium-binding EF hand domain Immunoglobulinlike domains (IgV, IgC1, IgC2, and IgI) are involved in protein–protein interactions that occur for cell ... often closely linked, as ligand binding associated with outside in signaling stimulates integrins and, conversely, integrin activation increases ligand binding for inside–out signaling 1.1.5.3 Modularity...
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ROUTLEDGE STUDIES IN SCIENCE, TECHNOLOGY AND SOCIETY: Human-animal relations and the rise of veterinary medicine pot
Ngày tải lên :
06/03/2014, 16:20
... problem of maintaining animal resources and protecting human health and economy This notion is central to my discussion and is intended to epitomise and encapsulate the growing and increasingly formalised ... about the increasing intensification of animal use for both animal and human health All those interested in human -animal relationships or in public health issues will find Animals, Disease and Human ... in recent times, been numerous attempts to explain why— after many thousands of years—our ancestors abandoned scavenging, hunting and gathering in favour of animal husbandry and agriculture In...
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Báo cáo khoa học: Expression of cholinesterases in human kidney and its variation in renal cell carcinoma types pdf
Ngày tải lên :
06/03/2014, 22:21
... between acetylcholinesterases of kidney and erythrocytes in the extent of binding with the lectins concanavalin A, Lens culinaris agglutinin 4522 (LCA), and Ricinus communis agglutinin (RCA) (Fig ... acetylcholinesterase and butyrylcholinesterase seem to play noncatalytic roles, which would explain their wide distribution in tissues and cells [16], including stem cells [17] Increasing evidence links ... amyloid b-peptide and presenilin-1 [20], neuronal enolase, the scaffold protein RACK1 and protein kinase C [21], the corepressor CtBP [22], and Ran-binding protein [23] Butyrylcholinesterase can...
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Báo cáo khoa học: A role for serglycin proteoglycan in granular retention and processing of mast cell secretory granule components ppt
Ngày tải lên :
07/03/2014, 11:20
... time points, pro-CPA dominated over mature protease, both intracellularly (Fig 3B; day 5) and in conditioned medium from SG+ ⁄ + cells (Fig 3C; days and 12), indicating that efficient processing ... purpose, cells were incubated with NH4Cl in order to raise the pH of acidic intracellular compartments, including lysosomes and secretory granules, and thereby inactivate lysosomal proteases Incubation ... b-hexosaminidase content in SG+ ⁄ + cells at day 33 is set as 100% Results are expressed as means of triplicate determinations ± SD detected in SG– ⁄ – cells, indicating that pro-CPA processing into...
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Báo cáo khoa học: Two functionally redundant isoforms of Drosophila melanogaster eukaryotic initiation factor 4B are involved in cap-dependent translation, cell survival, and proliferation doc
Ngày tải lên :
07/03/2014, 15:20
... cultures and in developing tissues provided interesting insights into the function of eIF4B In mammalian cell cultures, a 50-fold overexpression of eIF4B resulted in the inhibition of the protein synthesis ... of all cell types in the Drosophila eye Cell 87, 651–660 52 Kimmel, B.E., Heberlein, U & Rubin, G.M (1990) The homeodomain protein rough is expressed in a subset of cells in the developing Drosophila ... displayed in (E) and (F) show apical accumulation in the external and pole cells (H) and apical accumulation in tracheal pits (I) of the proteins (J) Immunohistochemistry of Schneider cells using anti-eIF4B...
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Báo cáo khoa học: The influence of cold shock proteins on transcription and translation studied in cell-free model systems pdf
Ngày tải lên :
07/03/2014, 21:20
... existing in vivo, the unspecic inhibition of protein expression observed in the living cell after a cold shock can be explained by a direct binding of CSP to RNA and or DNA Besides this, in our ... enzymes cleaving the target protein Other effects which can occur in whole cells (e.g the induction of new proteins interfering with the protein expression) cannot occur in our isolated in vitro ... DNA and or RNA is required and that protein protein interaction or increased nuclease activity can be excluded as the main inhibitory factors for the expression process For reversing the inhibition...
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