dhea androgen receptors and their potential role in breast cancer

... 7H11 plates containing either kanamycin (Km) or hygromycin (Hyg) Mice were killed or 21 days postinfection, and the mutant and wild-type loads in lungs and spleens were determined on the basis ... for each strain in a final volume of 500 lL The actual CFUs of each strain in both inocula were determined by plating serial dilutions on 7H11 plates containing either Km or Hyg before infection ... the spleen and lungs by homogenizing tissues in mL of NaCl ⁄ Pi containing 0.05% Tween-80, and the number of viable bacteria in the organs of infected mice were determined by plating serial dilutions...

Ngày tải lên: 23/03/2014, 09:20

... the in vivo expression of 15-LOX-1 and -2 proteins in OA cartilage 13-HODE and 15-HETE inhibited IL-1β-induced MMP-1 and MMP-13 expression in chondrocytes To examine the effects of 15-LOX-1 and ... IL-1β-induced MMP-1 and MMP-13 expression by interfering with key signalling pathways In this context, 15LOX metabolites were shown to inhibit protein kinase C activity and translocation [20,49], and ... expression For instance, IL-4 and IL-13, increase the expression of 15LOX-1 and -2 in a number of cell types, including monocytes/ macrophages, T lymphocytes and several cancer cell lines [40-45]...

Ngày tải lên: 09/08/2014, 14:20

... of spin-spin interaction, spin chains can be classified into three types: Heisenberg spin chain, XY spin chain and Ising spin chain In the following, we will describe each of the spin chains in ... Publications Vinitha Balachandran, and Jiangbin Gong, Adiabatic quantum transport in a spin chain with a moving potential, Phys Rev A 77, 012303 (2008) Vinitha Balachandran, and Jiangbin Gong, Controlled ... STUDIES OF QUANTUM SPIN CHAIN DYNAMICS AND THEIR POTENTIAL APPLICATIONS IN QUANTUM INFORMATION VINITHA BALACHANDRAN (M Sc., Cochin University of Science And Technology, India) A THESIS SUBMITTED...

Ngày tải lên: 09/09/2015, 18:51

... concentrations and s-creatinine, this could in part be explained by an impaired renal function in elderly patients, which is in line with previous findings demonstrating an association between increased ... been found to be involved in tissue repair in vitro [12] and to play an important role in the tumour microenvironment and tumour cell invasion [13] In the light of these findings, it could be ... proteins and activates other MSPs and MMPs [8,9] TATI is a trypsin inhibitor that balances concentrations of trypsin and also functions as a weak inhibitor of other serine proteinases [10,11] In...

Ngày tải lên: 09/08/2014, 09:21

... UNIVERSITY OF SINGAPORE 2005 Acknowledgement At the point of finishing my master candidature in Singapore and completing my thesis, I would like to thank the following organizations and people Firstly, ... Introduction 1.1 Background Brain cancer is caused by uncontrolled cell growth in the brain It can be divided into two categories: the primary brain cancer which is originated within the brain ... act as in vitro BBB model At present, there are more than 15 different cell line models, including immortalized bovine BEC lines, porcine BEC lines, murine cell lines, primary rat BECs and human...

Ngày tải lên: 26/11/2015, 22:41

... exercise training and natural killer cell cytotoxic activity in breast cancer patients Int J Sports Med 1995;16:334-7 Stoll BA Diet and exercise regimens to improve breast carcinoma prognosis Cancer ... other diseases, and extending survival in cancer survivors Preliminary research suggests that exercise may be an effective intervention for enhancing QOL in cancer survivors and that this effect ... few in number, however, and of limited quality Further research using rigorous methodology is needed to definitively answer questions concerning the role of exercise in enhancing QOL in cancer...

Ngày tải lên: 08/08/2014, 16:23

... Stefanick ML: Influence of a diet very high in vegetables, fruit, and fiber and low in fat on prognosis following treatment for breast cancer: the Women's Healthy Eating and Living (WHEL) randomized ... contraceptives and prognosis in breast cancer: effects of duration, latency, recency, age at first use and relation to parity and body mass Page of 39 40 41 42 index in young women with breast cancer ... new era in breast cancer Invasion, size, and nodal involvement dramatically decreasing as a result of mammographic screening Arch Surg 1996, 131(3):301-308 Fisher B, Slack NH, Bross ID: Cancer...

Ngày tải lên: 09/08/2014, 03:21

... essential roles in promoting breast cancer in general, including both BLBC and luminal breast cancer (Cleator et al., 2007; Santana-Davila and Perez, 2010) For example, PI3K/AKT/mTOR and Ras/MEK/MAPK ... emerged, for instance claudin-low breast cancer (Table 1.1 and Table 1.2) (Peddi et al., 2012) In this thesis, my focus will be on the basal-like and luminal breast cancer in general Intrinsic subtypes ... TSS UTR WT Xist Interleukin Interleukin Ingenuity pathway analysis NF-κB inhibitor α Jumonji D3 Lymphotoxin β Maltose-binding protein Methyl-CpG binding domain proteins Micro RNA Messenger RNA...

Ngày tải lên: 09/09/2015, 17:53

... also contain a carboxy-terminal extension containing T-box and A-box motifs [124] In each zinc finger, four invariant cysteine residues coordinate tetrahedrically to a zinc ion, and both zinc fingers ... overlapping ligand-binding pockets The difference of the binding pocket is easy to understand by looking at the superimposition between PonA and BYI06830 shown in Fig Ligand-binding affinity Typically, ... for retaining the ligand-binding activity They found two high-affinity binding sites (Kd1 = 0.24 nm and Kd2 = 3.9 nm) The removal of GST had no effect on PonA binding, but altered DNA binding The...

Ngày tải lên: 23/03/2014, 04:20

... This expanding IL-1R1-like family includes murine and human proteins, Drosophila (fruit fly) proteins, and a plant (tobacco) protein [7,8] In Drosophila, Toll is involved in the rapid and transient ... sections) Understanding the regulation of TLR expression will provide further insight into their tissue distribution and their role in combating infection There has been an initial report of ... (see Fig 3) Further evidence for the role of TRAF6 and NF-κB-inducing kinase in TLR4 signaling came from the study of TRAF6 and NF-κB-inducing kinase dominant-negative (DN) variants, which could...

Ngày tải lên: 12/08/2014, 18:21

... conditions [62] Interestingly, we observed that the insulin mRNA PTB binding site has an overlapping consensus CSD binding site PTB, to date, has only been implicated in stability of insulin and VEGF ... proteins may also play a role in post-transcriptional regulation of VEGF expression in the cytoplasm, in conjunction with another singlestrand RNA/DNA binding protein, PTB Conserved CSD/PTB binding ... (3¢) protein binding sites are underlined Both sequences are required for cytoplasmic CSD/PTB complex formation and recombinant CSD protein binding (B) A comparison of CSD protein RNA binding sites...

Ngày tải lên: 19/02/2014, 12:20

... than in OA and AS synovium CD147 was expressed predominantly on the macrophage-like cells and fibroblast-like synovial cells in the lining and sublining layers (Fig 2) MMP-1 was expressed predominantly ... CD147), matrix metalloproteinase-1 (RA MMP-1), tissue inhibitor of metalloproteinases-1 (RA TIMP-1) and blank contrast (RA blank) in the lining and sublining layers in RA synovium (× 400) The ... (green) and CD147+ (red) cells, and the co-expression of CD68+CD147+ cells (yellow) in the lining and sublining layers of rheumatoid arthritis synovium is apparent Immunohistochemistry staining of...

Ngày tải lên: 09/08/2014, 06:23

... ECM including dermatopontin (DPT), asporin (ASPN), biglycan (BGN), cartilage intermediate protein (CILP2), fibromodulin (FMOD), tenascin C (TNC) and fibronectin (FN1) showed a significant increase ... (Apotekets, Umeå, Sweden) and 1% penicillin-streptomycin (PEST, PAA Laboratories, Linz, Austria) The cells were centrifuged at 500 g for five minutes and maintained in 37°C in 7% carbon dioxide/93% ... study design and coordination AL and MS conceived the study and participated in its design and coordination All authors read and approved the final manuscript Additional files The following Additional...

Ngày tải lên: 09/08/2014, 14:22

... domain FL: Follistatin-like domain NtA: amino-terminal agrin domain LE: Laminin d id LE L i i and epidermal growth f t lik d l th factor-like domain i EL LGL B/z S/T: Serine/Threonine rich domain ... Agrin in motor neurons [23] These mice exhibit a reduced number of presynaptic and postsynaptic specializations in the brain, indicating that Agrin has a role, at least in part, in maintaining ... tissue remains unresolved Potential Agrin receptors in the immune system Agrin is a large HSPG containing many distinct structural domains (Figure  1), and to date several Agrin binding partners...

Ngày tải lên: 12/08/2014, 12:20

... fixed in buffered formalin, embedded in paraffin, and stained with hematoxylin-eosin-safran and Masson's trichrome Hepatic steatosis, stage of fibrosis and grade of disease activity were determined ... assessment: insulin resistance and bcell function from fasting plasma glucose and insulin concentrations in man Diabetologia 1985; 28:412-9 Farrell GC, Chitturi S, Lau GK, et al Guidelines for the ... without steatosis, and the results demonstrated significant increases in BMI, FBG, FINS, TG, TC, GGT, ALP, Glb and HOMA-IR in patients with steatosis, implying that obesity, diabetes and hyperlipemia...

Ngày tải lên: 25/10/2012, 11:48

... C-terminal epitope of Vgf Unoccupied binding sites on the plates were blocked by incubation with casein Samples and standards were applied in duplicate and incubated overnight at 4°C Following ... tissue staining intensities influenced the measured values Average value density measurements from individual Vgf immunoreactive dorsal spinal cord neurons, reflecting immunostaining intensity, ... delimiting the cellular area of interest free hand, using predetermined criteria to define the region of interest The immune intensity of the cellular Vgf encompassing the L3-L5 regions of spinal...

Ngày tải lên: 03/11/2012, 10:52

... events and storm runoff events which bring increases in pollutant loads during flooding-stages in large river catchments As the traverse water mixing in the wide Yodo River was relatively weak during ... points spanning the river during the five flooding-stages were analyzed individually, and the representative concentrations were determined as the arithmetical means for the three points The inorganic ... during the five flooding-stage observation periods became larger than those during only routine observations during the flooding periods (shown in Table1) In particular, peak times of flow and...

Ngày tải lên: 05/09/2013, 09:38

... species in 2003 and 15 species in 2004 were observed in Line (Table 1) U pinnatifida and crustose coralline algae were the dominant species A total of sea urchin species were observed in Line between ... flow conditions preventing the migration and/ or grazing by sea urchin even with their high abundance in the outer bay In contrast, in the inner areas (Line 2) where U pinnatifida no longer occurred, ... sea urchin 80 70 60 50 40 2003 30 2004 20 10 0 10 20 30 40 50 Density of sea urchin (ind m-2) 70 50 60 U pinnatifida (Line 1) U pinnatifida (Line 2) Sea urchin (Line 1) Sea urchin (Line 2) 50...

Ngày tải lên: 05/09/2013, 09:38

... Professor in 2006 He earned his B.S in Mathematics and Agricultural Engineering in India, a M.S degree in Food Engineering in Thailand, and completed his Ph.D in Agricultural Engineering from ... Bioprocess Engineering in the Department of Agricultural and Biosystems Engineering, South Dakota State University, Brookings, SD, USA He joined the faculty in 1997 as an Assistant Professor and was ... manufacturer’s instructions Protein was estimated by the Bradford method using bovine serum albumin as standard [38] All experiments were run in duplicate and standard deviations (SD) were calculated using...

Ngày tải lên: 05/09/2013, 16:11

... common fetal origin, perhaps involving mild deficiencies in androgen production/action during fetal masculinisation A number of ECs, including pesticides, chemicals in consumer products and persistent ... whereas in Afro-American men it is increasing (McGlynn et al 2005; Shah et al 2007) A similar trend is perhaps also emerging in Europe, as TGCT 13 incidence is increasing in Finland (where the incidence ... Procymidone) exert their antiandrogenic effects by binding to the AR – and then instead of activating it, sit there and block it and thus prevent activation of that receptor by endogenous androgens (Gray...

Ngày tải lên: 13/02/2014, 10:20