development of embryo sac from nucellar cells is called

... On the basis of the results obtained, we discuss the role of the HBD from Tma-RNase HI. Results Protein preparations The amino acid sequence of Tma-RNase HI is com- pared with those of the representative ... affinity of Tma-RNase HI is reduced by five- to seven-fold on removal of the HBD, regardless of the metal cofactor, and the large reduc- tion in Mg 2+ -dependent activity on removal of the HBD is not ... stability of Tma-W22A is not significantly changed even when the HBD is completely removed, and therefore it is highly likely that Tma-W22A is as stable as Tma-RNase HI. Discussion Importance of HBD...

Ngày tải lên: 06/03/2014, 22:21

... endothelial cells from various developmental stages and locations is important for dissecting molecular and cellular mechanisms of endo- thelial development and function. Murine primary cells, including ... ª 2008 FEBS Development of a new method for isolation and long-term culture of organ-specific blood vascular and lymphatic endothelial cells of the mouse Takashi Yamaguchi, Taeko Ichise, Osamu ... disease. Their properties have been studied extensively by in vivo analysis of genetically modified mice. However, further analysis of the molecular and cellular phe- notypes of endothelial cells...

Ngày tải lên: 18/02/2014, 17:20

... Mapping of the transcription start site of fgl2 mRNA. (A) 5¢-RACE of fgl2 mRNA from macrophages. Stars indicate the 5¢-ter- minus of 33 clones isolated after 5¢-RACE. (B) Primer extension analysis of ... that a variety of tissues may express basal levels of a TF/factor VII- independent procoagulant. Indeed, constitutive expression of TF in some cells and tissues is known to play this role, such as ... pathogenesis of MHV-3 induced fulminant hepatitis through its procoagulant activity [14,26]. We have also recently demonstrated that Fig. 7. EMSA analysis of the function of Ets/Stat3 cis elements....

Ngày tải lên: 20/02/2014, 11:20

... more frequently detected in cells from malignant tissues than those from normal tissues [24–33]. Mcm4-immunoreactivity was enhanced in the larger nuclei of cells of CIS compared with the cancer-free ... is not amplified in HeLa cells but there is some alteration of the Mcm2 gene structure in WI-38 cells. Protein synthesis of Mcm4 To further clarify why Mcm proteins are more abundant in HeLa cells ... HeLa cells to approximately four times the level in WI-38 cells, and the synthesis of Mcm4, 6 and 7 proteins was accelerated in HeLa cells. Immunohistochemical studies of surgical materials from...

Ngày tải lên: 20/02/2014, 23:20

... consists of 31 fractions. (B) Comparison of the profiles of 15 min (m)or40min(d) reoxygenated cells (same as in Fig. 2A) in percentage of total c.p.m. ·,Matured 14 C-labeled bulk DNA of T24 cells. ... exhibited sharp rise of mitotic cells 10 h after reoxygenation, which resembles the sharp rise in the DNA synthesis rate directly after reoxygenation. The Fig. 3. Histograms of cellular DNA content ... ascites cells. With these cells we first demonstrated the existence of the fast O 2 -dependent regulation of replication [12,22,23]. We had already developed means to select vital G 1 cells from...

Ngày tải lên: 21/02/2014, 00:20

... arabinanolytic genes [1]. The absence of expression of galA in the presence of pectic galactan indicates that galactose is not an inducer of galA expression. This is in agreement with a role for L -arabinose ... above. Expression of lacA is also detected in the presence of these compounds, but in the presence of D -xylose and xylan as well. This can be explained by the structure of the polysaccharides. Xylan ... Material and methods. Screening of a genomic library of A. niger using this fragment as a probe resulted in the isolation of two galA-containing phage k clones. From one of these clones a4.5-kbEcoRI...

Ngày tải lên: 21/02/2014, 01:21

... activity Hydrogen peroxide, the product of the SOD reaction, is an inhibitor of SODs and an inactivator of FeSOD. It is often used to distinguish between the two types of isoenzyme, particularly in conjunction ... 1. Comparison of E. coli mononuclear superoxide dismutase molecular features. (Top) Stereo view of the superposition of the backbone peptide chainofonesubunitofFeSOD(black)andMnSOD(grey)ofE. coli ... insertion of iron into the active site of SODs [3] (results not shown). Hydrogen peroxide inhibits MnSOD as it is the product of the enzymatic dismutation of the superoxide radical. In addition to this,...

Ngày tải lên: 21/02/2014, 01:21

... The major form of CAA consists of proteinaceous deposits of amyloid-b protein (Ab) that occur adjacent to vascular smooth muscle cells (VSMC). Ab consists of 39–43 amino acids and is proteolytically ... presence of the topoisomerase II inhibitor, etoposide, a potent inducer of apoptosis in many cells, approximately 40% of VSMC were observed to undergo apoptosis (P <0.001). Effect of Ab1–40 ... an increase in the number of apoptotic cells. This increase in apoptosis was accompanied by activation of caspase-3. Homocysteine, a known risk factor for cerebrovascular disease, increased Ab-induced...

Ngày tải lên: 22/02/2014, 07:20

... effects of the years of schooling of the head of the household, the years of schooling of the money manager and the average schooling of the heads of the village households are estimated. All of the ... part of its initial funding. The average loan size, the average duration of the loan, and the number of types of loans offered are insignificant. 13 is available is decreasing in the number of ... procedures and history. Some are purely the result of the desire of villagers to establish a bank. Others have received some outside support and technical assistance from the Ministry of Agriculture...

Ngày tải lên: 06/03/2014, 10:20

... Little is known about the active sites of M18 family proteins. We purified the protein complex of Yhr113wp from yeast cells and identified it as yeast aspartyl amino- peptidase on the basis of its ... N-terminal acidic amino-acid residues. This characteristic is shared by mammalian aspartyl aminopeptidases. Comparison of the digestion Table 1. Activities of purified yeast aspartyl aminopeptidase ... aminopeptid- ases lie within the same group of the dendrogram. Proteins in this group, such as those from Schizosac- charomyces pombe, Caenorhabditis elegans and Arabid- opsis thaliana, should exhibit aspartyl...

Ngày tải lên: 07/03/2014, 12:20

... definition is one of the main bot- tlenecks in the development of new ap- plications in the field of Information Ex- traction from text. Generic resources (e.g., lexical databases) are promising ... definition of lexica starting from a corpus sample (Riloff, 1993; Grishman, 1997) with the implicit assumption that a corpus provided for an application is representative of the whole applica- *This ... use of generic dictionaries with respect to the IE needs. First there is no clear way of extracting from them the mapping between the FL and the ontol- ogy; this is mainly due to a lack of...

Ngày tải lên: 08/03/2014, 21:20

... MALDI-TOF MS an alysis of a tryptic digest of the protein indicated for the N-terminal peptide MDSQVR a value of m/z 777, which points t o the presence of acetylme thionine. This r esidue is commonly found ... of two H-chain deletion mutants. J. Biol. Chem 26 3 , 18086–18092. 16. Macara, I.G., Hoy, T.G. & Harrison, P.M. (1972) The formation of ferritin from apoferritin k inetics and mechanism of ... than that of the L -rich apoferritin of horse spleen (t 1/2 ¼ 60 0 s) [19]. The iron-oxidation capacity is maintained by T. bernacchii apoferritin at low temperature; the rate of the reaction is r...

Ngày tải lên: 08/03/2014, 23:20

... in the presence of serum (C), and DNA isolated from floating and attached cells was analyzed by agarose gel electrophoresis. Total proteins isolated from cells in (B) or from cells in (C) were ... induced apoptosis of human tumor epithelial cells [21]. Accumulation of nCLU correlated with inhibition of cell proliferation and induction of apoptosis of human tumor epithelial cells caused by ... extracts isolated from confluent monolayers of HaCaT Neo and HaCaT c-fos cells and total proteins isolated from HaCaT cells and HaCaT cells expressing nCLU (C120) were analyzed for the expression of...

Ngày tải lên: 16/03/2014, 02:20

... remnants therefore may promote the development of atherosclerosis, and this is likely to be particularly important in conditions where clearance of remnants from the circulation is delayed. Keywords: chylomicron ... in macrophages, and that this is caused by a markedly increased rate of uptake of the particles and by a raised intracellular synthesis of triacylglycerol, but not of cholesteryl ester. Furthermore, ... induction of lipid accumulation in the cells by the particles is enhanced when they are protected from oxidation, we investigated the mechanism of this effect by comparing the influence of CRLPs...

Ngày tải lên: 16/03/2014, 16:20

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Ngày tải lên: 28/03/2014, 22:21