development of embryo sac from nucellar cells is called
Báo cáo khoa học: The N-terminal hybrid binding domain of RNase HI from Thermotoga maritima is important for substrate binding and Mg2+-dependent activity pot
... On the basis of the results obtained,we discuss the role of the HBD from Tma-RNase HI.ResultsProtein preparationsThe amino acid sequence of Tma-RNase HI is com-pared with those of the representative ... affinity of Tma-RNase HI is reduced by five- to seven-fold on removal of the HBD,regardless of the metal cofactor, and the large reduc-tion in Mg2+-dependent activity on removal of theHBD is not ... stability of Tma-W22A is not significantlychanged even when the HBD is completely removed,and therefore it is highly likely that Tma-W22A is asstable as Tma-RNase HI.DiscussionImportance of HBD...
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Tài liệu Báo cáo khoa học: Development of a new method for isolation and long-term culture of organ-specific blood vascular and lymphatic endothelial cells of the mouse pdf
... endothelial cells from variousdevelopmental stages and locations is important fordissecting molecular and cellular mechanisms of endo-thelial development and function.Murine primary cells, including ... ª 2008 FEBS Development of a new method for isolation and long-termculture of organ-specific blood vascular and lymphaticendothelial cells of the mouseTakashi Yamaguchi, Taeko Ichise, Osamu ... disease. Theirproperties have been studied extensively by in vivo analysis of geneticallymodified mice. However, further analysis of the molecular and cellular phe-notypes of endothelial cells...
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Tài liệu Báo cáo khoa học: Gene transcription of fgl2 in endothelial cells is controlled by Ets-1 and Oct-1 and requires the presence of both Sp1 and Sp3 pdf
... Mapping of the transcription start site of fgl2 mRNA. (A) 5¢-RACE of fgl2 mRNA from macrophages. Stars indicate the 5¢-ter-minus of 33 clones isolated after 5¢-RACE. (B)Primer extension analysis of ... that avariety of tissues may express basal levels of a TF/factor VII-independent procoagulant. Indeed, constitutive expression of TF in some cells and tissues is known to play this role, suchas ... pathogenesis of MHV-3induced fulminant hepatitis through its procoagulantactivity [14,26]. We have also recently demonstrated thatFig. 7. EMSA analysis of the function of Ets/Stat3 cis elements....
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Tài liệu Báo cáo khoa học: Enhanced expression of Mcm proteins in cancer cells derived from uterine cervix docx
... morefrequently detected in cells from malignant tissues thanthose from normal tissues [24–33]. Mcm4-immunoreactivitywas enhanced in the larger nuclei of cells of CIS compared with the cancer-free ... is not amplified in HeLa cells but there is some alteration of the Mcm2 gene structure in WI-38 cells. Protein synthesis of Mcm4To further clarify why Mcm proteins are more abundant inHeLa cells ... HeLa cells toapproximately four times the level in WI-38 cells, and thesynthesis of Mcm4, 6 and 7 proteins was accelerated in HeLa cells. Immunohistochemical studies of surgical materialsfrom...
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Tài liệu Báo cáo khoa học: Analyzing changes of chromatin-bound replication proteins occurring in response to and after release from a hypoxic block of replicon initiation in T24 cells pptx
... consists of 31 fractions. (B) Comparison of the profiles of 15 min (m)or40min(d) reoxygenated cells (same as in Fig. 2A) inpercentage of total c.p.m. ·,Matured14C-labeled bulk DNA of T24 cells. ... exhibited sharp rise of mitotic cells 10 h after reoxygenation, which resembles the sharp rise inthe DNA synthesis rate directly after reoxygenation. TheFig. 3. Histograms of cellular DNA content ... ascites cells. With these cells we first demonstratedthe existence of the fast O2-dependent regulation of replication [12,22,23]. We had already developed means toselect vital G1 cells from...
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Tài liệu Báo cáo Y học: The b-1,4-endogalactanase A gene from Aspergillus niger is specifically induced on arabinose and galacturonic acid and plays an important role in the degradation of pectic hairy regions pdf
... arabinanolyticgenes [1]. The absence of expression of galA in the presence of pectic galactan indicates that galactose is not an inducer of galA expression. This is in agreement with a role forL-arabinose ... above. Expression of lacA is also detected in thepresence of these compounds, but in the presence of D-xyloseand xylan as well. This can be explained by the structure of the polysaccharides. Xylan ... Material andmethods. Screening of a genomic library of A. niger usingthis fragment as a probe resulted in the isolation of twogalA-containing phage k clones. From one of these clonesa4.5-kbEcoRI...
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Tài liệu Báo cáo Y học: Thermostability of manganese- and iron-superoxide dismutases from Escherichia coli is determined by the characteristic position of a glutamine residue pdf
... activityHydrogen peroxide, the product of the SOD reaction, is an inhibitor of SODs and an inactivator of FeSOD. It is often used to distinguish between the two types of isoenzyme, particularly in conjunction ... 1. Comparison of E. coli mononuclear superoxide dismutase molecular features. (Top) Stereo view of the superposition of the backbone peptidechainofonesubunitofFeSOD(black)andMnSOD(grey)ofE. coli ... insertion of iron into the active site of SODs [3] (results not shown).Hydrogen peroxide inhibits MnSOD as it is the product of the enzymatic dismutation of the superoxide radical. Inaddition to this,...
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Tài liệu Báo cáo Y học: Toxicity of substrate-bound amyloid peptides on vascular smooth muscle cells is enhanced by homocysteine docx
... Themajor form of CAA consists of proteinaceous deposits of amyloid-b protein (Ab) that occur adjacent to vascularsmooth muscle cells (VSMC). Ab consists of 39–43 aminoacids and is proteolytically ... presence of the topoisomerase IIinhibitor, etoposide, a potent inducer of apoptosis in many cells, approximately 40% of VSMC were observed toundergo apoptosis (P <0.001).Effect of Ab1–40 ... anincrease in the number of apoptotic cells. This increase inapoptosis was accompanied by activation of caspase-3.Homocysteine, a known risk factor for cerebrovasculardisease, increased Ab-induced...
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Human Capital and the Development of Financial Institutions: Evidence from Thailand docx
... effects of the years of schooling of the head of thehousehold, the years of schooling of the money manager and the average schooling of theheads of the village households are estimated.All of the ... part of its initial funding. The average loan size, the averageduration of the loan, and the number of types of loans offered are insignificant.13 is available is decreasing in the number of ... procedures and history. Some are purely theresult of the desire of villagers to establish a bank. Others have received some outsidesupport and technical assistance from the Ministry of Agriculture...
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Báo cáo khoa học: Identification of yeast aspartyl aminopeptidase gene by purifying and characterizing its product from yeast cells potx
... Little is known about the active sites of M18 family proteins.We purified the protein complex of Yhr113wp from yeast cells and identified it as yeast aspartyl amino-peptidase on the basis of its ... N-terminal acidic amino-acidresidues. This characteristic is shared by mammalianaspartyl aminopeptidases. Comparison of the digestionTable 1. Activities of purified yeast aspartyl aminopeptidase ... aminopeptid-ases lie within the same group of the dendrogram.Proteins in this group, such as those from Schizosac-charomyces pombe, Caenorhabditis elegans and Arabid-opsis thaliana, should exhibit aspartyl...
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Báo cáo khoa học: " The Development of Lexical Resources for Information Extraction from Text Combining Word Net and Dewey Decimal Classification" potx
... definition is one of the main bot- tlenecks in the development of new ap- plications in the field of Information Ex- traction from text. Generic resources (e.g., lexical databases) are promising ... definition of lexica starting from a corpus sample (Riloff, 1993; Grishman, 1997) with the implicit assumption that a corpus provided for an application is representative of the whole applica- *This ... use of generic dictionaries with respect to the IE needs. First there is no clear way of extracting from them the mapping between the FL and the ontol- ogy; this is mainly due to a lack of...
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Báo cáo Y học: Ferritin from the spleen of the Antarctic teleost Trematomus bernacchii is an M-type homopolymer doc
... MALDI-TOF MS an alysis of atryptic digest of the protein indicated for the N-terminalpeptide MDSQVR a value of m/z 777, which points t o thepresence of acetylme thionine. This r esidue is commonlyfound ... of two H-chaindeletion mutants. J. Biol. Chem 26 3 , 18086–18092.16. Macara, I.G., Hoy, T.G. & Harrison, P.M. (1972) The formation of ferritin from apoferritin k inetics and mechanism of ... thanthat of the L -rich apoferritin of horse spleen (t1/2¼ 60 0 s)[19]. The iron-oxidation capacity is maintained byT. bernacchii apoferritin at low temperature; the rate of the reaction is r...
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Báo cáo khoa học: Vanadium-induced apoptosis of HaCaT cells is mediated by c-fos and involves nuclear accumulation of clusterin pptx
... in the presence of serum (C), and DNA isolated from floating and attached cells was analyzed by agarose gel electrophoresis. Total proteins isolated from cells in (B) or from cells in (C) were ... induced apoptosis of humantumor epithelial cells [21]. Accumulation of nCLUcorrelated with inhibition of cell proliferation andinduction of apoptosis of human tumor epithelial cells caused by ... extracts isolated from confluentmonolayers of HaCaT Neo and HaCaT c-fos cells and total proteinsisolated from HaCaT cells and HaCaT cells expressing nCLU (C120)were analyzed for the expression of...
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Báo cáo khoa học: Protection of chylomicron remnants from oxidation by incorporation of probucol into the particles enhances their uptake by human macrophages and increases lipid accumulation in the cells ppt
... remnantstherefore may promote the development of atherosclerosis,and this is likely to be particularly important in conditionswhere clearance of remnants from the circulation is delayed.Keywords: chylomicron ... in macrophages, and that this is causedby a markedly increased rate of uptake of the particles andby a raised intracellular synthesis of triacylglycerol, but not of cholesteryl ester. Furthermore, ... induction of lipidaccumulation in the cells by the particles is enhanced whenthey are protected from oxidation, we investigated themechanism of this effect by comparing the influence of CRLPs...
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- development of embryo sac animation
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Tìm thêm: xác định các mục tiêu của chương trình xác định các nguyên tắc biên soạn khảo sát các chuẩn giảng dạy tiếng nhật từ góc độ lí thuyết và thực tiễn khảo sát chương trình đào tạo gắn với các giáo trình cụ thể xác định thời lượng học về mặt lí thuyết và thực tế tiến hành xây dựng chương trình đào tạo dành cho đối tượng không chuyên ngữ tại việt nam điều tra đối với đối tượng giảng viên và đối tượng quản lí điều tra với đối tượng sinh viên học tiếng nhật không chuyên ngữ1 nội dung cụ thể cho từng kĩ năng ở từng cấp độ xác định mức độ đáp ứng về văn hoá và chuyên môn trong ct mở máy động cơ lồng sóc mở máy động cơ rôto dây quấn các đặc tính của động cơ điện không đồng bộ đặc tuyến mômen quay m fi p2 đặc tuyến dòng điện stato i1 fi p2 động cơ điện không đồng bộ một pha thông tin liên lạc và các dịch vụ phần 3 giới thiệu nguyên liệu chỉ tiêu chất lượng theo chất lượng phẩm chất sản phẩm khô từ gạo của bộ y tế năm 2008 chỉ tiêu chất lượng 9 tr 25