development of a high throughput enantiomeric excess ee screening assay

... TGTTACCGCG 481 AAACACGGCG TGGTGGGCAT GACCAAAAGC GCCGCCGTTG ATTATGCCAA AAAAGGCATC 541 CGCGTTACCG CGGTTGGCCC GGGCTTTATT GATACCCCGC TGCTGAGCGC CCTGCCGAAA 601 GAAACCCTGG ATTATCTGAA AAGCGTGCAT CCGATTGGTC ... ATAGCTTTGA TGATTGGCAT CGTGTGATCG ATGTGAACCT GAACAGCGTG 361 TTTTATAGCA TGAAATATGA AATTGTTGCC ATGCTGCGCG CGGGTGGCGG CGCCATTGTG 421 AACATGGCGA GCATTCTGGG TAGCGTGACC TTTCCGAATG CGCCGGCCTA TGTTACCGCG 481 AAACACGGCG ... Ni-NTA gravity flow column ATGAGCCAGG ATTTCAGCGG CAAAGTGGCC TTTGTGACCG GTGGCGCGAG CGGCATTGGT 61 GAAGCCGTGG TGAAACAGCT GGCGGCGCGT GGTGCGAAAG TTGTGGTTGC GGATCTGAAA 121 CTGGAAGGTG CGCAGGCCGT GGCGGATGCG...

Ngày tải lên: 12/09/2015, 10:22

... oxidoreductase activity in Arabidopsis thaliana P1 f-crystallin Eur J Biochem 267, 3661–3671 50 Yamazoe, M., Shirahige, K., Rashid, M.B., Kaneko, Y., Nakayama, T., Ogasawara, N & Yoshikawa, H (1994) A ... may also function as allyl ADHs as they have 70% sequence identity to a protein from Nicotiana tabacum that acts on monoterpene allylic alcohols [53] The LTD active sites all are deep and narrow ... significant, and is marked with an asterisk in Fig Each protein that was not assigned an unambiguous placement during the bootstrap tests was manually investigated for the appearance of family...

Ngày tải lên: 21/02/2014, 03:20

... L lactis MG1363 and FI9078 were amplified by PCR with primers LdhB1 (5¢-GTAATTATCATAGAGAGTTTTTAGGAG-3¢) and LdhB2 (5¢-CAAATCCTGTTCCAATCACGA-3¢), designed on the basis of available sequences of ... by AGOWA GmbH (Berlin, Germany) Sequence data were assembled with clone manager (Scientific & Educational Software, Cary, NC, USA) and analyzed with the blast program available at the National ... pH 6.0 and in the absence of Fru(1,6)P2, Pi was an activator of LDHB with a Kact of 2.0 ± 0.5 mm Pi was nearly as effective as Fru(1,6)P2 for activation of LDHB, insofar as the maximal activity...

Ngày tải lên: 16/03/2014, 05:20

... TCATCTCTGCTCTTCCACCCTCCAAAGACGCAGCCCTTCCACGTACGCC CCCAGCACAGAACACCCAGCTCTCTGGATCTCAAAATGTCAGGACAGTCCG CATCATCTCTGCTCTTCCAACCACCAAAGACGCAGCCCTTCCATGTCCG GTGATATCAGAGAACACTGTCAGGAACAAGGCTTCAGGTCACGGTCGC GGCCTTCACAGCTTTGTCAACATCTGCCTTGTCCCCTTCTTCCACATGGC ... TGGCCTAGAACTGCAGGAAGAGGCGTGAACAGGGATCCACTAACCGCGT CTCTCCACACTCTTCCATCCTCCAAAGGCGGTGCCTTTCCATGTGCGTC GACACTCTCCACACTCTTCCAGCCTCCAAAGGCAGTGCCTTTCCACGTG TCATCTCTGCTCTTCCACCCTCCAAAGACGCAGCCCTTCCACGTACGCC ... Adh) Aldh1 (class I Aldh) – Mouse Rat Rat Mouse Rat Mouse Rat Rat Rat Rat ⁄ mouse Rat ⁄ mouse ACAGCCAATGATGACAGACAGACCGACACCTCCGAGGCCAAACACGGC GGTTAACGGAGAGGCTTTGGGCACTGGGAGGCACCCCGACAATGACGCT...

Ngày tải lên: 16/03/2014, 11:20

... preference among archaean, bacterial, and eukaryotic genomes Kingdom FAD NAD NADP Archaea Bacteria Eukaryota 0.21 0.21 0.21 0.49 0.46 0.41 0.30 0.33 0.38 Among the annotated human Rossmann proteins, ... respectively The bacterial genome of Chlamydophila caviae also show a dual sites proportion of 15%, while the archeal genomes of Thermococcus kodakaraensis and Nanoarchaeum equitans show 17 and 20%, respectively ... Thermococcus kodakaraensis, Candida glabrata and Yarrowia lipolytica, the Rossmann proteins are two to three times more redundant than proteins in general The redundancy among eukaryotes increases with...

Ngày tải lên: 23/03/2014, 10:21

... between Ca atoms of MCAD and those of the other four ACAD structures that have been Fig A stereo view of the active site cavity of MCAD The cavity surface is shown with a green transparent surface ... of the ACADs Peroxisomal acyl-CoA oxidase Peroxisomal acyl-CoA oxidases (ACOs) are the peroxisomal equivalent of the mitochondrial acyl-CoA dehydrogenases (ACADs) They are flavoenzymes containing ... that, again, the overall polypeptide fold and its substrate-binding mode are very similar to that of MCAD [6] As in the case of SCAD, the acyl-CoA binding cavity is shallower than that of MCAD...

Ngày tải lên: 23/03/2014, 12:20

... CAT SLC2 2A5 CACT SLC2 5A2 CPTI CPT 1A CPTII CPT2 VLCAD ACADVL LCHAD HADHA LCKAT HADHB MCAD ACADM SCHAD HADHSC SCAD ACADS ETF ETFA ETFB ETFDH ETFDH Exon cDNA Large Out of frame StopConsensus Total ... for clinically relevant transport proteins and the enzymes of mitochondrial fatty acid oxidation, as available in publicly accessible databases developed and maintained at the National Center ... 3-hydroxyacyl-CoA dehydrogenase and long-chain 3-oxoacyl-CoA thiolase); MCAD, medium-chain acyl-CoA dehydrogenase; SCHAD, short-chain 3-hydroxyacyl-CoA dehydrogenase; SCKAT, short-chain 3-oxoacyl-CoA thiolase;...

Ngày tải lên: 23/03/2014, 12:20

... background activity, measured for several minutes before adding the substrate to start the assay The linearity of the assay was monitored over time by applying the standard assay (= complete assay ... inhibition of the lactococcal dehydrogenases and ADH of yeast and horse liver as a function of the ATP and ADP pool Criterion for all data points chosen: [ATP] > [ADP] (A) Dehydrogenases from Lactococcus ... by ATP and ADP (Fig 4) Especially through decreased values of the dissociation constants and a low value of a (Table 2), ADH became more strongly inhibited than GAPDH only at high levels of ATP...

Ngày tải lên: 29/03/2014, 08:20

... Cardozo J, Casagrande EC, Ferrari F, Franco SF, Giachetto PF, Girasol A, Jordão H Jr, Silva VHA, Souza LCA, Aguilar-Vildoso CI, Zanca AS, Arruda P, Kitajima JP, Reinach FC, Ferro JA, Silva ACR: ... Identification and Genomic Characterization of a New Virus (Tymoviridae Family) Associated with Citrus Sudden Death Disease J Virol 2005, 79:3028-3037 Satyanarayana T, Gowda S, Mawassi M, Albiach-Martí ... Phylogenetic and recombination analysis of 286 RdRp gene fragments sampled from three Brazilian orange trees Phylogenetic and recombination analysis of 286 RdRp gene fragments sampled from three Brazilian...

Ngày tải lên: 20/06/2014, 01:20

... Glutama dehydrogenases in ectomycorrhizas of spruce (Picea excelsa L.) and beech (Fagus sylvatica L.) New Phytol 111, 683-692 Khalid A. , Boukroute A. , Botton B & Martin F (1988) The aspartate aminotransferase ... mycorrhiza, which exhibited a high level of NAD-GDH activity as did the non-mycorrhizal roots (Fig B) As for aspartate aminotransferase, the distinct isoforms found in mycorrhizas, always corresponded ... the free-living fungus Hebeloma sp a high level of NADP-GDH activity was found, whereas only NAD-GDH activity was detected in non-mycorrhizal roots In the association spruce-Hebefoma, both activities...

Ngày tải lên: 09/08/2014, 04:20

... and humans all have three Class I ADHs (ADH 1A, ADH1B, and ADH1C); orangutans appear to only have two (ADH 1A and ADH1C) [Figure 5] As demonstrated in Figure 5, humans and chimpanzees (both of which ... value was similar to that of ethanol and propanol oxidation and approximately 2-fold higher than for that of hexanol and cyclohexanol oxidation [Table 8; Figure 11 -A] The Vmax/Km value for trans-2-hexen-1-ol ... The Vmax measured with trans-2-hexen-1-ol as a substrate was higher than that of all primary alcohols—increasing by a maximum of about 2-fold (relative to pentanol/hexanol) [Table 8] The Vmax/Km...

Ngày tải lên: 24/08/2014, 12:25

... proximal promoter 4Basal ADH4 proximal promoter ADH alcohol dehydrogenase ALDH aldehyde dehydrogenase ANOVA analysis of variance AP-1 activator protein-1 Arg arginine bp base pair C/EBP CCAAT/ enhancer ... United States (Mokdad et al., 2004) Diagnostic criteria for alcoholism have been defined in Diagnostic and Statistical Manual of Mental Disorders (DSM) and International Classification of diseases ... that is affected by ethanol is AMPK (AMP- activated protein kinase) Activation of AMPK leads to fatty acid oxidation and concurrent inhibition of fatty acid synthesis (Hardie et al., 1998) AMPK...

Ngày tải lên: 24/08/2014, 12:37

... trademark of GoAhead Software, Inc Ⅲ RTLinux is a registered trademark and FSMLabs, RTLinuxPro and RTCore are trademarks of Finite State Machine Labs, Inc Ⅲ Debian is a registered trademark of ... registered trademarks and ARM7 and ARM9 are trademarks of Advanced RISC Machines, Ltd Ⅲ AMD is a registered trademark of Advanced Micro Devices, Inc Ⅲ Intel and Pentium are registered trademarks and i386 ... Windows, WinCE and Microsoft are registered trademarks and MS-DOS and DirectX are trademarks of Microsoft Corporation Ⅲ Solaris and Java are registered trademarks and ChorusOS is a trademark of Sun Microsystems,...

Ngày tải lên: 04/08/2012, 14:23

... Taiwan Upon arrival in Taiwan, DDGS was put into 50 kg bags and stored in a covered steel pole barn for 10 weeks during the course of the dairy feeding trial on a commercial dairy farm located about ... syrup balls among the samples collected Table 3: Mean and Variation of Particle Size Among Ethanol Plants and Bulk Density of DDGS in 2001 Plant 8a 8b 10 12 13 14 15 16 Average Particle Size Mean ... the average particle size (geometric mean), the coefficient of variation (CV) and 08 - Physical & Chemical Characteristics of DDGS standard deviation (SD) of particle size within and among ethanol...

Ngày tải lên: 08/08/2012, 10:03

Ngày tải lên: 08/08/2012, 17:20

... Advertising Manager Arbitrator Area / Regional Manager External Auditor Foreman Graduate Trainee IT Consultant Marketing Manager Official Mediator Official Receiver Sales Representative Security Guard ... admonish analyse appoint assess at assign audit avert await annul appeal to award We need to examine in detail the market potential of these new products The value of the business was calculated ... set, a stereo and a DVD player (c) A refrigerator, a dishwasher and a washing machine (d) A car, an air-conditioning unit and a bathroom suite (e) A carton of milk, 250g of cheese and a bottle of...

Ngày tải lên: 08/08/2012, 17:31

... eleven, half of us had zonked out (b) By half past eleven, half of us had gone home 27 (a) I'm gagging for a cup of tea (b) I really want a cup of tea 28 (a) The rail company flogged off half its ... suggest that you read this important information about phrasal verbs and idioms What is a phrasal verb? A phrasal verb is a verb formed from two (or sometimes three) parts: a verb and an adverb ... haven't got any coffee, I'm afraid We'll have to tea (to deal with a situation by using what is available rather than using what you want) The boss spent all morning writing out a list of ...

Ngày tải lên: 08/08/2012, 17:32

... for a given application 1.5.1 Packaging The ATmega16 comes in three different packaging styles: a 40-pin plastic dual in-line package (DIP), a 44-lead thin quad flat pack package, and a 44-pad ... Programmable Flash EEPROM Bulk programmable flash EEPROM is used to store programs It can be erased and programmed as a single unit Also, should a program require a large table of constants, it may ... data on the physical layout and operating parameters of the ATmega16 microcontroller As a system designer, it is important to know the various physical and operating parameter options available...

Ngày tải lên: 16/08/2012, 09:03

... FullName}" /> Themes are logical packages of styles, control templates, and data templates that define the look and feel of an application All WPF applications will have a default theme, ... of Technology, India His professional skills and contributions are in the fields of GIS Software and Custom Application Development, Advanced Spatial Database and Data Structure, Computational ... properties—it can also read and write to collections of data There are also a number of parameters available on a per-binding basis that allow customization of the minutiae of certain binding requirements...

Ngày tải lên: 17/08/2012, 08:54