determination of iup based on susceptibility for degradation by default

... đàn Con 400 100 - Đực giống Con 51 12,75 + Đực ngoại Con 27 6,75 + Đực Móng Cái Con + Đực hậu bị Con 20 - Lợn nái Con 94 23,5 + Nái ngoại Con 17 4,25 + Nái Móng Cái Con 71 17,75 + Nái F1 Con 1,5 ... nuôi giống Con 61 15,25 - Lợn thịt Con 194 48,5 Bò: chủ yếu bò lai Sind - Tổng đàn Con 85 100 + Bò Cái Con 57 67,1 + Bò đực Con 2,4 + Bê Con 26 30,5 Gia cầm - Tổng đàn Con 3550 100 + Gà Con 3500 ... cho như: Cystin thay 50% nhu cầu methionine lợn Ví dụ: Nhu cầu Methionine 0,6% 0,3 % Methionine Cystin bù đắp Cystin methionine axit amin chứa lưu huỳnh Methionine tổng hợp từ Cystin phải cung cấp...

Ngày tải lên: 31/10/2012, 14:03

... III at a concentration of 17 lm, a SAM concentration of 200 lm and at NirE concentrations of 1.5 lm Under these assay conditions a specific activity of 316.8 nmol of precorrin-2 h)1Æmg)1 of NirE ... used at a final concentration of lm The reaction was started by the addition of SAM to a final concentration of 200 lm and was incubated at 37 °C in the dark The reaction was monitored using a ... at 17 °C After 18 h of culture the cells were harvested by centrifugation and stored at )20 °C until required Construction of vectors For the construction of nirE expression vectors, the nirE...

Ngày tải lên: 18/02/2014, 06:20

... dependence of hMS activity on MSR concentration, illustrating the optimal concentration of MSR required for reductive methylation (reactivation) of the cob(II)alamin form of hMS hMS assays were performed ... of hMS activity on NADPH concentration, illustrating the optimal concentration of NADPH required for reductive methylation (reactivation) of the cob(II)alamin form of hMS hMS assays were performed ... reaction was quenched by the addition of 0.2 mL of a solution containing 11 m formic acid and m HCl, and heated to 90 °C for 10 The acidification of the reaction mixture quantitatively converts...

Ngày tải lên: 18/02/2014, 08:20

... contained 50 mm phosphate buffer (pH 7.4), 227 lm 14C-5-methyltetrahydrofolate Determination of Km and Vmax for 5-methyltetrahydrofolate Assays were incubated for 10 at a fixed concentration of ... establish conditions for linear kinetics The concentration of protein used was then adjusted to ensure that the initial velocities were correctly estimated Determination of Km and Vmax for homocysteine ... MetS was constructed In the absence of a high-resolution structure of the methyltetrahydrofolate-binding site for the mammalian enzyme, a model based on the X-ray crystal structure of the methyltetrahydrofolate...

Ngày tải lên: 19/02/2014, 05:20

... Ras is regulated by a series of post-translational modifications, including farnesylation, palmitation, and nitrosylation, but the role of these modifications on the regulation of downstream effectors ... effects of these mutations on cPHB accumulation are consistent with their effects on atoDAEB operon expression, i.e the mutation of Asp has a more potent phenotype than the substitution of His, ... content by 16% and 35% on the 4th and the 7th day of incubation with the drug, respectively However, on the 11th day of incubation with this drug cellular ATP formation recovered In conclusion,...

Ngày tải lên: 19/02/2014, 07:20

... quantitative contribution in vivo where the concentration of cysteine is low (15 lM), this reaction contributed signicantly to the production of pyruvate under our conditions, where the concentrations of ... constant in the absence and presence of a saturating concentration of AdoMet, respectively K1K2 is the product of the binding constants for the association of the rst and the second molecule of ... substrate consumption In addition, the concentration of cysteine was set at 250 lM rather than 15 lM (physiological concentration) Indeed, it was difcult to achieve a constant concentration of cysteine...

Ngày tải lên: 20/02/2014, 02:21

... enhanced emission in the presence of ADP or ATP can only be a consequence of conformational changes caused by the binding of the nucleotide to the enzyme active site region Binding of CO2 (expressed ... of saturating concentrations of Mn2+, the intrinsic uorescence was quenched by 10% In spite of this small effect of PEP on the Trp emission, the uorescence decrease as function of free PEP concentration ... of the contribution of the slow component This result points to changes in the dynamical properties of the local environment of the P-pyridoxyl group due to changes of the protein conformation...

Ngày tải lên: 21/02/2014, 01:21

... SAH production, which in turn could be used for the formation of polyamines and methionine An investigation of the level of different intermediate metabolites and the expression of different ... precursor molecule for polyamine formation (aminopropylation), and functions as the sole methyl group donor in various other transmethylation reactions, as needed for creatine and glutathione synthesis ... produced by AGAT and arginase II In these tumor cells, SAH hydrolase and methionine synthase mRNA levels are high, indicating the activation of the pathway for the utilization of SAH and formation of...

Ngày tải lên: 07/03/2014, 04:20

... kcat for Cys This implies that the hydroxyl group of Tyr108 of EhMGL1 is actively involved in the b-elimination and c-elimination of the C–S bond, but not the b-elimination of the C–O bond, of ... in the degradation of Met under normal conditions Similarly, the 2.0-fold higher kcat and 2.7fold lower Km for Cys of EhMGL1 than of EhMGL2, together with the intracellular Cys concentration ± ... the degradation of TFM, is less sensitive than EhMGL1 seems to contradict the notion that the product of the degradation is the enzyme inactivator However, we speculate that the distribution of...

Ngày tải lên: 07/03/2014, 05:20

... green conformer A and B of the (cc) structure, respectively The position of wat5 which H-bonds to the side chain of T98 is indicated [blue (rt) structure; yellow, conformer A; green, conformer ... cryo-temperatures leads to the visualization of two conformers On comparing the main-chain torsion angles and coordinates of the two conformers it is apparent that conformer B has almost identical geometry ... cyt c2 have a profound effect on the kinetics of the rearrangement of the ‘open’ and ‘closed’ forms of the ligand loop [19] Therefore, conformer A may be considered as the open form, albeit with...

Ngày tải lên: 07/03/2014, 17:20

... two kinds of distortion probability in model 4: one for head words and the other for non-head words Both of the distortion probabilities describe the distribution of relative positions Thus, ... is the distortion probability for the j head word of cept i d >1 ( j − p( j )) is the distortion probability for the non-head words of cept i h(i ) = min{k : i = a k } is the head of cept i k p( ... Association for Computational Linguistics (ACL-2003), pages 88-95 Michael Collins and Yoram Singer 1999 Unsupervised Models for Named Entity Classification In Proc of the Joint SIGDAT Conference on...

Ngày tải lên: 08/03/2014, 02:21

... lexicon construction In COLING-ACL '98: 36th An- nual Meeting of the Association for Computational Linguistics and 17th International Conference on Computational Linguistics: Proceedings of the Conference, ... this kind of problem since only the most common hypernym of an entire cluster of nouns is reported, so much of the noise is filtered Conclusion We have shown that hypernym hierarchies of nouns ... other, have hypernym-hyponym relationships of their own, or be completely unrelated.) If a hypernym has occurred with only one of the descendant nouns, it is not listed as one of the best hypernyms,...

Ngày tải lên: 08/03/2014, 06:20

... and an S0.5-value of 40 lM (Table 1) The feedback inhibition of MAT by AdoMet, was analyzed using the Dixon approach at L-methionine concentrations of 0.5–5.0 mM, resulting in a noncompetitive pattern ... After induction, growth was continued for h Cells were harvested by centrifugation, washed with saline solution, and stored at )70 °C until use Cell pellets were disrupted by sonication at °C with ... scintillation counter using 10 mL of Optiphase-Hisafe (Wallac) cocktail for aqueous mixtures One unit of MAT activity is defined as the amount of enzyme that catalyses the formation of lmol AdoMet...

Ngày tải lên: 08/03/2014, 08:20

... Incubation was carried out with 5±20 lg protein in 24 lL of reaction mixture at 30 °C for 1±10 The reaction was then stopped by adding mL of ice cold H2O Extraction of tetrapyrroles was performed ... They were cloned in NdeI and BamHI restriction sites of pET-Y3a to express truncated forms of the MgPIXMT Preparation of recombinant proteins BL21(DE3) strain of E coli was transformed with pET-Y3a ... orientation Enzyme activity was measured in bacterial pellet obtained from 200 lL of culture (B) Comparison of MgPIXMT activity and protein composition of bacteria h after of induction P corresponds...

Ngày tải lên: 08/03/2014, 16:20

... intensity of staining was probed as a function of 10–100 lg of labeled polymalate At low concentration, the success of staining depended on the time of injection in the cell cycle The majority of nuclei ... the time of injection Staining by the overlay method as in Fig or by microinjection of 10 lg or less of the conjugate resulted in the same type of fluorescent nuclei that was typical for early ... oil immersion objective lens, no additional magnification in front of the camera, a piezoelectric z-axis focus device (resolution 10 nm) and computer-controlled excitation light shutter For optimal...

Ngày tải lên: 17/03/2014, 10:20

... into the role of the DUF1253 domain on Utp25p function, made by testing the complementation of growth of the conditional strain Dutp25 ⁄ GAL::UTP25 with deletion mutants expressing only the DUF1253 ... presence of EDTA Levels of 40S subunit are strongly decreased upon depletion of Utp25p Numbers indicate area quantitation of subunits peaks Based on the Utp25p nucleolar localization and its possible ... sedimentation of mature rRNAs 25S and 18S were used as controls Western blot with antiserum against Rpl5p was performed as a control (B) The effect of Utp25p depletion on the sedimentation of U3 snoRNA...

Ngày tải lên: 22/03/2014, 21:21

... region This region, containing helices a10–a11, is one of the more conserved regions of the activation domain It contains many buried ionic and polar residues, forming an extended network of salt ... discussion of the calculations can be found in of Lawrence and Colman [24] These calculations were done on both the dimer interface and between symmetry-related monomers in a nondimeric conformation ... site of the activation domain Other known clinical mutations of the human activation domain include H920D, E1204X (early termination), and insertion ⁄ deletion mutations [21] The suggestion of...

Ngày tải lên: 23/03/2014, 09:21

... supervision for relation extraction Freebase contains 116 million instances of 7,300 relations between million entities The intuition of distant supervision is that any sentence that contains a pair of ... successful systems, for example by Etzioni et al (2005) Other work 1004 Relation name /people/person/nationality /location/location/contains /people/person/profession /people/person/place of birth /dining/restaurant/cuisine ... Relation name /location/location/contains /location/location/contains /music/artist/origin /people/deceased person/place of death /people/person/nationality /people/person/place of birth /book/author/works...

Ngày tải lên: 23/03/2014, 16:21

... IL-11 is able to induce the formation of osteoclasts in bone morrow and able to stimulate bone resorption [58] Inhibition of ligand binding by monoclonal antibodies Monoclonal anti-(IL-11) Ig, anti-gp130 ... nevertheless not contradictory because IC50 values are not intrinsic constants as they depend on the concentration of ligand used; the higher concentration of ligand used, the larger concentration of inhibitor ... the expression of the recombinant protein was induced by addition of mM isopropyl thiob-D-galactoside for h E coli cells were then harvested and lyzed by sonication for at an intensity of level...

Ngày tải lên: 24/03/2014, 00:21