detection of viral micrornas by northern blot analysis

... Number of Number of Number Number of Number Number of Number Results r of mice cleared mice cleared of of mice mice cleared of of mice mice cleared of of mice of monitoring mice of trypanosomes ... Number of Number Number of Number Number of Number Results r of mice cleared mice cleared of of mice mice cleared of of mice mice cleared of of mice of monitoring mice of trypanosomes killed trypanosomes ... Number of Number Number of Number Number of Number Results r of mice cleared mice cleared of of mice mice cleared of of mice mice cleared of of mice of monitoring mice of trypanosomes killed trypanosomes...

Ngày tải lên: 28/04/2014, 13:08

... Evaluation of Performance of Mutual Fund Strategies by Comparison of Efficient Frontiers—Joint Exemplar DEA 173 Section 3.4 Step Four: Performance of Mutual Fund Strategies by Comparison of Efficient ... begins by reviewing the modern development of the definition of efficiency in economic analysis and the development of DEA from the basic idea of efficiency Then the method for strategic analysis ... elaboration of the classes of decisions made by managers, the explicit separation of strategy from goals and objectives, and the concept of risk as a function of the amount and extent of knowledge,...

Ngày tải lên: 03/06/2014, 01:55

... expression of thousands of genes [9,12,13] Another challenge is that the specificity and sensitivity of a given RNA profiling platform are affected by the abundance and variability of the globin ... an improved detection sensitivity of nearly 4-fold, a reduction of the globin artifact by 5-fold and an increase in statistical power (signal to noise) of more than 3-fold The loss of correlation ... and editing of the manuscript SL completed the analysis of the protocol selection study, participated in the analysis of the SAHA data and participated in the drafting and editing of the manuscript...

Ngày tải lên: 18/06/2014, 16:20

... delay interval from 10 to 12.5 microseconds) Mean of number of detections per scan inside the interface vicinity Standard deviation of number of detections per scan inside the interface vicinity ... computed the mean number of detections per A-scan inside the interface vicinity (i.e., total number of detections inside the vicinity divided by 75, the total number of A-scans) and the corresponding ... gating of the original signal, and gating of the prewhitened original signal The use of a matched filter requires knowledge of the waveform of the signal which is to be detected Gating of the...

Ngày tải lên: 22/06/2014, 19:20

... cardiac-enriched microRNAs to different extents Twenty microRNAs were significantly up or downregulated by at least 1-fold in SRF-Tg versus wild-type mice (Figure 3B) Real-time RT-PCR analysis of microRNAs ... that of wildtype mice (p < 0.05*, n = 3) 4B Altered expression of microRNAs occurred at as early as days of age, and continued to months of age in SRF Tg mice miR-21 was increased in the heart of ... quantitation of pri-miRNAs and mature RNAs Detection of pri-miRNAs The primers for the detection of pri-miRNAs were designed using PRISM Primer Express 3.0 software (Applied Biosystems), and synthesized...

Ngày tải lên: 10/08/2014, 05:21

... deletions of the 3' LTR of different lengths and the corresponding viral expression patterns Various Various molecular clones with deletions of the 3' LTR of different lengths and the corresponding viral ... expression patterns (A) Diagram of the molecular clones showing the deletion series of the 3' LTR and the deletion of the reverse TATA box of the 3' LTR (B) The amount of p19 gag in the pooled concentrated ... with clones containing deletions of the 3' LTR of different lengths and the deleted reverse TATA box 2-3 days after transfection The results of RT-PCR detection of tax mRNA in cells transfected...

Ngày tải lên: 12/08/2014, 04:21

... Similarity between the structures of retroviral capsids Similarity between the structures of retroviral capsids Superimposition of the structures of the N terminal domains of HIV-1 (Red) and MLV (blue) ... AY523867 Figure Similarity between the sequences of retroviral capsids Similarity between the sequences of retroviral capsids Alignment of primate lentiviral capsid protein sequences demonstrates ... antiviral factor was responsible was suggested by the demonstration that the block to infection could be saturated, or abrogated, by high doses of retroviral cores [4-6] The putative human antiviral...

Ngày tải lên: 13/08/2014, 05:22

... only to detection and identification but also to the monitoring of viral populations In 1999, we and colleagues [6] described the first viral DNA microarray for the temporal profiling of viral ... identification of viral inhibitors [6], and in 2002 this idea was applied by Wang et al [4] to the detection and identification of viruses The authors [4] described the use of viral DNA microarrays ... hybridization signatures of viral sequences that effectively serve as viral barcodes’ for the identification of known, related or novel viruses By taking advantage of the highly conserved regions...

Ngày tải lên: 14/08/2014, 14:21

... and C value of SVM during optimization 71 vii List of Tables LIST OF TABLES Table 4.1 Function of components of limbic system 34 Table 4.2 The conductivity (S/m) of tissues below ... of independent components fall into both sides of the anterior temporal, clear correlation between the number of independent components on both sides of the anterior temporal and the status of ... my parents i Table of Contents TABLE of CONTENTS ACKNOWLEDGEMENT I TABLE OF CONTENTS II SUMMARY .IV LIST OF FIGURES VI LIST OF TABLES ...

Ngày tải lên: 22/10/2015, 21:19

... concentration of 10−10 M; the limit of detection (LOD) is estimated around fM (see Section 2) The linear part of the calibration curve corresponds to an extremely high sensitivity of +7.5% per ... electrochemical detection of miR141, a miRNA biomarker It is shown that the copolymer electroactivity is enhanced by the presence of o-MWCNTs, which probably participate to the low detection limit ... based on SWNTs and an immobilized redox probe This system allows very specific detection of DNA but the limit of detection is only 0.1 mM 3D structures obtained using CNTs may induce high capacitance...

Ngày tải lên: 02/07/2014, 14:14

... negative samples D F Figure Detection of NPM1 mutations and FLT3-ITD using high resolution melting analysis (A) The melt curve of NPM1 exon 12 and (B) The difference plot of NPM1 exon 12 Six patient ... juxtamembrane domain of FLT3 define a new class of activating mutations in AML Blood 2006, 107:3700-3707 Vaughn CP, Elenitoba-Johnson KS: High-resolution melting analysis for detection of internal tandem ... (5'UTR not included) ¶ The size of the internal tandem duplication could not be determined due to the low levels of mutant peaks in the sequence, profile in one of the two tested amplicons, were...

Ngày tải lên: 10/08/2014, 22:20

... simultaneous mutation detection in a functional domain of all three homoeologous genes in hexaploid wheat HRM analysis is an extension of previous DNA melting (dissociation) analysis enabled by the new ... directions, and then analyzed by Mutation Surveyor® Software The initial analysis identified 26 mutants (Additional file 1) An example of a mutant sequence trace analyzed by the software is shown in Figure ... identified eight mutants in the ABD6-1 region with seven detected by HRM analysis and one not detected by HRM of ABD6-1, but detected by HRM of ABD12-22 HRM on fragment ABD12-22 had better sensitivity...

Ngày tải lên: 12/08/2014, 03:21

... rate of false positives by a factor of because the variance in repeat number and the heterozygosity of a population measure different aspects of the variation at a locus Thus, combinations of any ... Disequilibrium Analysis Software (MIDAS; [38]) Values of D′ were calculated for all syntenic marker pairs on BTA1 and BTA20 across the populations A more detailed description of the estimation of D′ can ... Results Page of 14 also shows matrices of LD significance levels for all possible locus combinations of the loci on BTA1 or BTA20 in their chromosomal order Of the 120 pairwise comparisons of the 16...

Ngày tải lên: 14/08/2014, 13:21

... large inventory of normalized mathematical indexes of abnormality It is the pattern of these mathematical indexes of abnormality, obtained from analysis of multiple cardiac cycles of the resting ... likelihood of myocardial ischemia due to coronary stenosis Following the MCG manufacturer’s recommendation, a cut-off of 4.0 for the severity score was used in this meta -analysis; a score of 4.0 ... assess the performance of the prediction of stenosis independent of the prevalence of stenosis, the positive and negative likelihood ratios (LR) were calculated [24] A value of p

Ngày tải lên: 03/11/2012, 10:58

... GVi i (1) i DI: detection index of water sample DIi: detection index of compound i DVi: detected concentration value of compound i [mg/L] GVi: goal value of water quality control of compound i ... detected and quantified by two measurements at two sites In the Water Supply Law of Japan, 102 kinds of agricultural chemicals have been controlled by the total of their detection index DI that ... concentrated test solution and purged by nitrogen gas at a flow rate of 0.6 L/min The test solution was preserved by freezing it prior to analysis The illustration of the SPE procedure used in this...

Ngày tải lên: 05/09/2013, 10:15

... Table of Contents Page i Acknowledgements ii Table of contents Part I: Introduction 1.Justification of the Study 2.Aims of the Study 3.Scope of the Study 4.Methods of the Study 5.Design of the ... discourse analysis and the theory of presupposition Chapter two provides an overview of ideological discourse analysis Chapter three deals with an analysis of president Ho Chi Minh’s declaration of ... ideological discourse analysis should be seen as one specific type of socio-political analysis of discourse Such an 16 analysis attempts to relate structures of discourse with structures of society For...

Ngày tải lên: 14/12/2013, 00:41

... competition A lot of professional surfers thought that she was going to be the women‟s world champion That is the main content of the text The following is the analysis of the nouns formed by suffixes ... Aims of study Nouns formed by suffixes are an extremely difficult topic and it is easy to be confused Being aware of this fact, my graduation paper is aimed at: - Giving a clear analysis of nouns ... study, Methods of the study and Design of the study Part II – Development includes four main chapters such as Literature Review, Practical Background, An analysis of nouns formed by suffixes in...

Ngày tải lên: 14/12/2013, 16:45

... Description of Data collection 2.5 Procedure 2.6 Preliminary results and data analysis 2.6.1 Results of section of test 2.6.2 Results of section of test 2.6.3 Results of section of test 2.6.4 Results of ... Table of contents: Acknowlegements Symbols and abbreviations List of tables Table of contents Part 1: IntRoduction Rationale of the study Aims of the study Subjects of the study Scope of the ... looks new today Several of the dishes have been washed Most of the students are intelligent Most of food has been eaten (2) Concord of verb and title of work as subject Titles of individual books,...

Ngày tải lên: 18/12/2013, 10:04

... consequences of unsuccessful language" There are steps of error analysis: - Identification of errors - Description of errors - Explanation of errors - Evaluation of errors - Correct of errors Error analysis ... Error analysis is the study and analysis of the errors made by second or foreign language learners One of the functions of errors analysis is to identify the causes of the learners errors, which ... understanding of language competence Meanwhile mistakes are caused by the inappropriate use of language in the term of social acceptability or language performance 1.5.3 Error analysis Error analysis...

Ngày tải lên: 18/12/2013, 10:04

... Rationale of the study 1.2 Aims of the study 1.3 Research questions of the study 1.4 Subjects of the study 1.5 Procedure of the study 1.6 Scope of the study 1.7 Method of the study 1.8 Design of the ... been done on error analysis in the area of ellipsis Therefore, based on the outcomes of the previous contrastive analysis studies concerning cohesive devices, an analysis of errors in using ellipsis ... errors are caused by lack of knowledge about the target language, or by incorrect hypotheses about it; and mistakes are caused by temporary lapses of memory, confusion, slips of tongue and so...

Ngày tải lên: 20/12/2013, 18:16