detection of gases chemicals dna viruses proteins and cells
Multi wall carbon nanotubes (MWCNTs) doped polypyrrole DNA biosensor for label free detection of genetically modified organisms by QCM and EIS
Ngày tải lên :
02/07/2014, 14:14
... screening in DNA detection approach (this approach is more often used than the second one of protein detection because DNA stability is much higher than that of proteins and therefore DNA detection ... diameter of MWCNT is in the range of 40–60 nm and with a length of 1–2 m All aqueous solutions were prepared using Milli-Q water (18 M cm) 1165 Table Composition of PBS buffer and sequences of used ... formation and morphology Normally, sensitivity and reproductibility of DNA sensors are determined by the surface chemistry of the recognition interface, therefore immobilization of DNA probe...
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Báo cáo y học: "Comparative study between the Hybrid Capture II test and PCR based assay for the detection of human papillomavirus DNA in oral submucous fibrosis and oral squamous cell carcinoma" pdf
Ngày tải lên :
12/08/2014, 01:22
... value (RLU of specimen/ mean RLU of PC) was 1.0 pg/ml of sample Interpretation of Cut off value (RLU of specimen/mean RLU of PC) Cut-off ratio of to 0.99 is negative for HR HPV; Cutoff ratio greater ... off ratio at 1.0 correspond to viral DNA load of 5,000 copies/ml of 1pg/ml at a threshold of finding a clinical disease or prognosis of an OSMF and OSCC lesions DNA extraction and diagnosis of ... chemiluminiscent detection Procedure of the HC-II test First, double stranded DNA was denatured by using a strong alkaline denaturation solution and converted into single stranded DNA (ssDNA) This ssDNA was...
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Báo cáo y học: "Detection of Chlamydia trachomatis-DNA in synovial fluid: evaluation of the sensitivity of different DNA extraction methods and amplification systems" pps
Ngày tải lên :
12/08/2014, 11:22
... organizational aspects of the study, collection of clinical samples, culture of C trachomatis and performed DNA extraction, PCR analysis and drafted the manuscript IB and SM performed parts of DNA extraction ... evaluated samples of SF DNA in order to reconfirm or simply repeat results We therefore addressed the question of how detection limits of chlamydial DNA might change after storage of DNA depending ... negative during PCR and LCR analysis Influence of storage of DNA on sensitivity of detection limits of C trachomatis EB and C trachomatis PBMO DNA In routine diagnostic settings, it might become...
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Báo cáo khoa học: Identification of a preferred substrate peptide for transglutaminase 3 and detection of in situ activity in skin and hair follicles pdf
Ngày tải lên :
29/03/2014, 21:20
... activity in both human and mouse skin [16,19] These studies have provided new insight into the substrate specificity of Tgases and have expanded the range of application of the enzyme reaction ... localization of activated TGase 3, which was found to display a pattern distinct from that of TGase 1, was observed by the detection of in situ activities using this peptide Results Screening of candidate ... Thus, TGase and TGase appeared active in distinct regions of the hair follicle cells Discussion During differentiation of keratinocytes and hair formation, isopeptide cross-linking of several...
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Báo cáo hóa học: " Real-time detection of musical onsets with linear prediction and sinusoidal modeling" pot
Ngày tải lên :
20/06/2014, 22:20
... library of onset -detection software, which includes a database of hand-annotated musical note onsets, which was created as part of this study This database was adopted to assess the performance of ... sum of the five LP error signals and the amplitude envelope from the sixth band Page of 13 Our approach differs in a number of ways In this article we show that LP can be used to improve the detection ... onset detection written in C ++ and Python, and contains implementations of all of the ODFs discussed in this article in both programming languages In addition, from Python, there is onset detection...
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báo cáo hóa học: " Real-time detection of musical onsets with linear prediction and sinusoidal modeling" pptx
Ngày tải lên :
21/06/2014, 00:20
... library of onset -detection software, which includes a database of hand-annotated musical note onsets, which was created as part of this study This database was adopted to assess the performance of ... sum of the five LP error signals and the amplitude envelope from the sixth band Page of 13 Our approach differs in a number of ways In this article we show that LP can be used to improve the detection ... onset detection written in C ++ and Python, and contains implementations of all of the ODFs discussed in this article in both programming languages In addition, from Python, there is onset detection...
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Báo cáo khoa học: "Detection of Bartonella species from ticks, mites and small mammals in Korea" pps
Ngày tải lên :
07/08/2014, 18:21
... *remirP syassa RCP dna sremirp editoelcunogilO nim rof Co27 ta noisnetxe lanif fo elcyc eno dna nim rof Co27 ,s 09 rof Co55 ,nim rof Co59 fo selcyc pets eerht-53 yb dewollof nim rof Co59 ta noitarutaned ... dna revoc dnuorg tserof dna dnalssarg gniggalf dna gniggard yb detcelloc erew skciT gnilpmas lammam llams dna etim ,kciT sdohteM dna slairetaM aeroK ni slammam llams sa llew sa srotcev etim dna ... nim rof Co27 ta noisnetxe lanif a dna nim rof Co27 ta noisnetxe dna s 54 rof Co55 ta gnilaenna ,s 03 rof Co59 ta noitarutaned fo selcyc 53 yb dewollof nim rof Co59 ta noitarutaned laitini fo...
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Báo cáo khoa hoc:" Detection of quantitative trait loci for growth and fatness in pigs" ppt
Ngày tải lên :
09/08/2014, 18:21
... standard deviations of the 14 traits studied Trait Body weight (kg) at: - birth (WB) - weeks of age (W3w) - 10 weeks of age (W10w) - 13 weeks of age (W13w) - 17 weeks of age (W17w) - 22 weeks of ... (BF14w), 17 (BF17w) and 22 (BF22w) weeks of age; • average backfat thickness at 40 (BF40kg) and 60 (BF60kg) kg live weight The number of records, overall means and standard deviations of the 14 traits ... parents i and j to offspring k, and µxqs and i xq µij d can be parameterised as µx1j) = µi( j) +αx j) /2 and µx2j) = µi( j) −αx j) /2, αx i i( i( i( i( and αx being the within-half-sib and within-full-sib...
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Báo cáo y học: "Early Characterization of Toll-like receptors in primary lung epithelial cells: strong impact of the TLR3 ligand poly(I:C) on the regulation of Toll-like receptors, adaptor proteins and inflammatory response" ppt
Ngày tải lên :
11/08/2014, 08:21
... regulation of the expression of the known TLR adaptor proteins in small-airway epithelial cells To examine the impact of different TLR ligands on the expression of the adaptor proteins, cells were ... in pathogen recognition and induction and regulation of the innate and adaptive immune response Airway epithelial cells express TLRs and activation of TLRs on epithelial cells has been shown to ... and protein expression and leads to the down-regulation of TLR1, TLR5 and TLR6 expression There is a strong synergism of IFN-γ and TNF-α that results in a highly elevated expression of TLR2 and...
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Báo cáo sinh học: "Detection of quantitative trait loci for reproduction and production traits in Large White and French Landrace pig populations" docx
Ngày tải lên :
14/08/2014, 13:22
... xed eects of sow parity, month of farrowing, and herd*year*type of mating (i.e AI or natural mating) combination, the age of the sow within parity as a covariate, and the random eects of the boar ... side of the spine, cm from the middorsal line at the shoulder, last rib and hip joint) recorded at the end of the on-farm test (at 148 days of age and 95 kg on average) on male and female candidates ... presents the design and methods used for this detection, and reports the rst results of this study MATERIAL AND METHODS 2.1 Animals and measurements The experiment was based on a granddaughter design...
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Posttranscriptional regulation of erythropoiesis by RNA binding proteins and microRNAs
Ngày tải lên :
10/09/2015, 09:23
... results of GATA-1, FOG-1, and GATA-2 for G1E and G1E-ER4 cells are shown (C) The ChIP-PCR results of GATA-1 and FOG-1 in fetal liver and MEL cells are shown (D) The ChIP-PCR results of polII ... expansion of RPS19 deficient human CD34+ cells Haploinsufficiency of RPS19 is a characteristic of DBA, and the RPS19 knockdown model recapitulates the growth defects of the erythroid cells of DBA ... understanding of self-renewal of adult stem and progenitor cells is limited In different tissues and organs, a few molecules have been identified as important for self-renewal of adult stem and...
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Selection of aptamers for signal transduction proteins and development of highly sensitive aptamer probes
Ngày tải lên :
14/09/2015, 08:26
... ADNA / ( Adiss + ADNAãP )) [DNA] 0 + ( Adiss + ADNAãP ) / ADNA Where [P]o and [DNA] o are the initial concentrations of P and DNA in the equilibrium mixture ADNA is the peak area of the free DNA; ... = DNA + DNA P + P Direction of the migration in electrophoresis EM t1 DNA D N A DNA dissociated DNA from DNAP P DNA dissociated from DNAP DNAP P dissociated from DNAP P P dissociated from DNAP ... complex is defined as Kd = [ADNA ãAP]/ADNAãP whereby ADNA, AP and ADNAãP are the equilibrium 15 Chapter Introduction concentrations of free DNA, P and DNA P complex The amount of each component present...
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Detection of biologically relevant anions by fluorescence and NIR molecular probes
Ngày tải lên :
30/09/2015, 06:14
... presence and absence of HE .36 2.2.10 Determination of acid dissociation constants (pKa) of flavonols.37 2.2.11 Measurement of oxidation potentials of flavonols .37 2.2.12 Determination of DNA cleavage ... Results and discussion .140 6.3.1 Synthesis of complexes and 140 6.3.2 Spectroscopic characterization of complexes and .142 6.3.3 Comparison of NIR band shift for [Fe2]4+, 1, and ... a convenient way for the detection of the HS− generation rate of a H2S donor of medical importance The addition of π-acceptor ligands such as cyanide and isocyanide ligands to the NIR active isovalent...
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Báo cáo y học: " Early detection of Varicella-Zoster Virus (VZV)-specific T-cells before seroconversion in primary varicella infection: case report" potx
Ngày tải lên :
12/08/2014, 04:20
... relevant clinical and diagnostic parameters of the reported case are depicted in Figure (Fig 1) Conclusions The development of novel assays for the detection of virus-specific T -cells will contribute ... publication of this case report and any accompanying images A copy of the written consent is available for review by the Editor-in-Chief of this journal Additional file 1: Protocol for the detection of ... project and wrote the paper RH performed all FACS analyses for the detection of VZV-specific T cells JE collected the patient samples MGVP, K-IP and AP participated in the production of recombinant...
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Functional interactions of protein tyrosine phosphatase alpha (PTPa) and src in mouse development and integrin singaling investigation of double PTPa src deficient mice and cells
Ngày tải lên :
14/09/2015, 12:02
... PTPα-/-, Src-/-, and α/s DKO fibroblasts…………………………… ………………100 6.1 Summary of phenotypes of PTPα-/-, Src-/-, and PTPα-/-Src-/- mice ………….142 6.2 Summary of phenotypes of PTPα-/-, Src-/-, and α/s DKO ... or all of its functions are effected through dephosphorylation and activation of Src and/ or Fyn 1.5.3 Combinatorial regulation of PTPα catalytic activity and specificity Although the ligands for ... SFK members are expressed in T or B cells T cells primarily express Lck and Fyn-T (a hematopoietic isoform of Fyn); while B cells mainly express Lyn, Blk, and Fyn (Lowell et al., 1996) Evidence...
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Genotoxicity of 255 chemicals in the Salmonella microsome test (Ames test) and 8-hydroxyguanine (8-OH-Gua) assay for the detection of carcinogens
Ngày tải lên :
05/09/2013, 08:40
... genotoxicity of chemicals 8-OH-Gua is one of the most critical lesions generated from deoxyguanine (dG) in oxidized DNA which was observed after reaction of DNA, protein and lipids both in vitro and in ... P.P and Kadluber, F.F (1987) Identification of C8-modified deoxyinosine and N2- and C8-modified deoxyguanosine as major products of the in vivo reaction of N-hydroxy-6-aminochrysene with DNA and ... 2,4,5-trichlorophenol and triphenyltin (IV) chloride 8-OH-Gua assay 4-NQO (21 chemicals) Not detected (151 chemicals) Ames test (83 chemicals) YG1041 (21 chemicals) YG1042 (51 chemicals) YG3003 (8 chemicals) ...
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Detection of bacteria and enteric viruses from river and estuarine sediment
Ngày tải lên :
05/09/2013, 10:15
... ISOIL for Beads Beating (Nippon Gene, Tokyo) Detection of pathogens and fecal indicators by PCR and qPCR methods The concentration of (c )DNA of Poliovirus and Adenovirus was determined using real-time ... mean) of Poliovirus type (RNA viruses) and Adenovirus type 41 (DNA viruses) in sediment Error bars show geometric standard deviation of recovery rates 100% 90% Composition rate [%] 80% Medium sand ... with ethidium bromide, and visualized by UV illumination Evaluation of recovery rate of preparation method for viruses in sediment As a surrogate of RNA viruses, Sabin strain of Poliovirus type was...
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Báo cáo khoa học: DNA mediated disassembly of hRad51 and hRad52 proteins and recruitment of hRad51 to ssDNA by hRad52 pot
Ngày tải lên :
16/03/2014, 14:20
... of only ssDNA level of larger ones, thereby corroborating the effect of ssDNA induced disaggregation of hRad51 DNA/ ATP induced changes in hRad51 We tested whether DNA induced disaggregation of ... recruitment of hRad51 to ssDNA The results described in this study help us to understand the transitions associated with the oligomeric states of hRad51 and hRad52 proteins in the presence of ssDNA and ... Disassembly and recruitment of hRad51 and hRad52 proteins shorter and thicker nucleoprotein filaments when ssDNA is added [28] Tumour suppressors BRCA2 and p53 interact with the oligomerization domain of...
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Báo cáo khoa học: Detection of nucleolar organizer and mitochondrial DNA insertion regions based on the isochore map of Arabidopsis thaliana ppt
Ngày tải lên :
30/03/2014, 20:20
... homogeneity of isochore can be defined by an index h [17,20], which is defined as the variance of GC content of the region divided by that of the whole genome If h ( 1, the variance of GC content of the ... orange dots in Fig 1, and they are located in 0–230 kb of chromosomes II and 0–350 kb of chromosomes IV, respectively The similar genomic organization of chromosomes I, III and V makes their overall ... arms and often most abundant in percentromeric DNA [16] The uninterrupted repeat arrays may up to more than Mb in the centromere region of each chromosome [23] and the unsequenced regions of centromeres...
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in situ detection of dna damage, methods and protocols
Ngày tải lên :
11/04/2014, 09:47
... iodide signal (DNA content) on the X axis and TUNEL labeling (DNA breaks) on the Y axis 3.3 Determining the Nature of the Ends of the Fragments 3.3.1 Processing of Cells and Extraction of DNA Each ... point on each strand of the DNA duplex to generate multiple smaller fragments each with 3'OH and 5'-P ends In B the endonuclease cleavage of each strand of the DNA duplex is offset generating ... precipitated DNA in 50 µL of TE buffer in a microfuge tube (see Note 19) 3.3.2 Labeling of 3'-OH Ends of DNA Prepare a reaction tube by adding 25 µL of 2× TdT reaction buffer, µL of sample from step of...
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