detection of dental calculus and caries
Xpert MTB/RIF test for detection of pulmonary tuberculosis and rifampicin resistance (Protocol) pptx
... percentage of contaminated cultures; review question B: the name and manufacturer of the reference standard • Details of index test: software version of test • Details of comparator: type of microscopy: ... 2010a) Early and accurate diagnosis and effective treatment is the cornerstone of TB care and control (Dye 2010) A basic tenet of early and accurate diagnosis is the identification of smear-negative ... addresses question A (detection of MTB) and/ or B (detec- - A tion of RIF resistance)? 2-B - A and B What was the purpose of this study? * if smear results are stratified, - Only accuracy of Xpert as replacement...
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Báo cáo khoa học: Detection of nucleolar organizer and mitochondrial DNA insertion regions based on the isochore map of Arabidopsis thaliana ppt
... homogeneity of isochore can be defined by an index h [17,20], which is defined as the variance of GC content of the region divided by that of the whole genome If h ( 1, the variance of GC content of the ... orange dots in Fig 1, and they are located in 0–230 kb of chromosomes II and 0–350 kb of chromosomes IV, respectively The similar genomic organization of chromosomes I, III and V makes their overall ... [5,7] Carels and Bernardi analyzed the contigs of A thaliana and concluded that the GC level of genes and coding regions, as well as gene densities and expression level showed to be evidently higher...
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Báo cáo khoa học: " Improved Automatic Detection of Zero Subjects and Impersonal Constructions in Spanish" docx
... precision of 74% and recall u of 60%, and K-nearest neighbors in TiMBL: both in (Evans, 2001) with precision of 73% and recall of 69%, and in (Boyd et al., 2005) with precision of 82% and recall of ... definitions and values tense, and mood with the preceding verb in the sentence and also with the main verb of the sentence.3 7-9 NHPREV, NHTOT, INF: the candidates for the subject of the clause ... Alexander Clark, Chris Fox, and Shalom Lappin, editors, The handbook of computational linguistics and natural language processing, pages 599–629 Wiley Blackwell, Oxford C M¨ ller 2006 Automatic detection...
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Báo cáo sinh học: "Concomitant detection of IFNa signature and activated monocyte/dendritic cell precursors in the peripheral blood of IFNa-treated subjects at early times after repeated local cytokine treatments" doc
... evaluate and compare the modulations of PBMC global gene expression profiles induced by IFNa in melanoma and normal donors The effects of the administration of different doses of IFNa, as well as of ... level of refractoriness and can occur after inoculation of as little as millions of units of IFNa iii) The observed molecular signature is paralleled by the raise in percentage and expression of ... few of these genes showed a trend of increase also in monocytes and PBMC exposed in vitro to IFNg, the induction was much stronger in terms of log ratio levels and more consistent among groups of...
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Báo cáo y học: "Targeted next-generation sequencing of a cancer transcriptome enhances detection of sequence variants and novel fusion transcript" ppt
... levels, facilitated the discovery of novel splice variants, and enabled detection of novel fusion transcripts and isoforms thereof that would otherwise have escaped detection As such, this method ... understanding of the molecular state of the transcriptome Finally, this method is easily scalable to larger numbers of samples and genes Our targeted RNA-Seq method provides a direct and powerful ... primers (Eurofins MWG Operon, Huntsville, AL, USA) as described earlier For random priming, μg mRNA and 1.5 μg random hexamers were used, and for gene-specific priming, 500 ng mRNA and pmol gene-specific...
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báo cáo khoa học: "C60-Fullerenes: detection of intracellular photoluminescence and lack of cytotoxic effects" pdf
... presence of a heterogenous mixture of variably sized clusters Furthermore, following evaporation of methanol, the majority of fullerene clusters not reaggregate, and have a range of sizes of tens of ... Photoluminescence of crystalline C60 occurs due to coupling of the vibrational modes of the lattice with electronic transitions and the PL signature of fullerene crystals may be useful to track the presence of ... indicative of C60 preparations in methanol and toluene (Figure 2) The observed mass is consistent with the formation of a positively charged C60 ion by loss of an electron instead of gain of a proton...
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Báo cáo y học: " The development of a rapid SYBR one step real-time RT-PCR for detection of porcine reproductive and respiratory syndrome virus" potx
... Page of Figure The specificity and sensitivity of one step SYBR green real-time RT-PCR Plot of the amplification of a 10-fold serial dilution of ch-1a RNA to calculate the detection limit and ... serially to 10-7 and were assayed in a 25 μl reaction mixture containing μl of diluted RNA; 0.4 μM of Nf primer and 0.4 μM of Nr primer; 12.5 μl of 2× One Step SYBR RT-PCR Buffer; μl of PrimeScript™ ... RT-PCR for detection of porcine reproductive and respiratory syndrome virus Virology Journal 2010, 7:90 Figure The sensitivity of conventional RT-PCR for detection of the PRRSV The detection limit...
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Báo cáo khoa học: " Detection of NP, N3 and N7 antibodies to avian influenza virus by indirect ELISA using yeast-expressed antigens" pptx
... Dybkaer K, Munch M, Handberg KJ, Jorgensen PH: Application and evaluation of RT-PCR-ELISA for the nucleoprotein and RTPCR for detection of low-pathogenic H5 and H7 subtypes of avian influenza virus ... N3 and N7 from yeast (0.2 N NaOH) and it did not have any negative effect on the quality of the product This method is simple and relatively inexpensive and bypasses the necessity of the microfluidizer ... was expanded in uracil deficient drop-out media containing 2% galactose and was grown to an OD600 of 0.8 Large scale cultures of 20 L were collected and the pellet was cracked with microfluidizer...
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Báo cáo khoa học: " A real-time RT-PCR for detection of clade 1 and 2 H5N1 Influenza A virus using Locked Nucleic Acid (LNA) TaqMan probes" pps
... plasmid, and less than 0.5 PFU of H5N1 viruses per reaction, and specific for the detection of influenza A of subtype H5 The HA gene of clades 1, 2.1, and 2.3 was amplified from both virus isolates and ... infection and H5N1 subtyping Results Analytical sensitivity and specificity The analytical sensitivity of our LNA Taqman rRT-PCR for the detection of the HA gene of H5N1 was < 0.5 PFU of virus and ... http://www.virologyj.com/content/7/1/46 Page of Conclusions We have developed a highly sensitive and specific rRTPCR assay for the detection of H5N1 influenza A virus of both clade and directly in clinical specimens, and evaluated...
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Báo cáo y học: " Multi-faceted, multi-versatile microarray: simultaneous detection of many viruses and their expression profiles" pps
... instance, cell lines latently infected with HIV-1 and KSHV were used to generate profiles of viral gene expression in the presence of cyclin-dependent Page of (page number not for citation purposes) ... post-translational modifications of histone proteins Methylation, acetylation, and/ or phosphorylation of certain amino acid residues at the amino terminal "tails" of histone H3 and/ or H4 can indeed influence ... the high throughput and flexible synthesis nature of DNA microarray construction can allow scientists to tailor-make and rapidly alter arrays to match evolving emergence of new pathogens The...
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Báo cáo y học: "Segmentation-based detection of allelic imbalance and loss-of-heterozygosity in cancer cells using whole genome SNP arrays" ppsx
... mBAF threshold of 0.97 and a triplet sum threshold of 0.8 for Illumina data For Affymetrix data, we used an mBAF threshold of 0.9 and a triplet sum threshold of 0.6 Segmentation of allelic proportions ... 1) (b) Copy number profile of chromosome with CBS segmentation profile superimposed in red Gains (red bars) and losses (green bars) are called by comparison of the CBS profile to log2-ratio thresholds ... on the basis of their segmented mBAF values Evaluation and comparison of sensitivity and specificity using simulated Illumina data To investigate the sensitivity and specificity of the segmentation...
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Development of gold nanoparticle DNA nanostructure assembly for detection of DNA, RNA and protein biomarkers
... synthesis of AuNPs of 15nm size and smaller In the work by Handley, the combination of tannic acid as a co-reductant with citrate resulted in AuNPs of 10nm size and below with good monodispersity and ... characteristics of the duplexes, and are of important consideration in the design and use of DNA-AuNP probes in the various detection systems discussed previously On the other hand, the type of DNA conformation ... the use of poly-G DNA, quadruplex formation, and modulation by molecular hairpins, AuNP assemblies was achieved and then optimized This was followed by the proof of concept detection of the let7a...
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Applications of malliavin calculus and white noise analysis in interest rate markets, and convertible bonds with and without symmetric informaiton
... expiry dates of zero-coupon treasure bonds Without loss of generality , we assume that we have a liability of one dollar at time U and the standard times be < T1 < T2 < T3 < TN And we want to ... “Applications of Malliavin Calculus and White Noise Analysis in Interest Rate Markets, and Convertible Bonds with and without Symmetric Information” by Wong Man Chui in partial fulfillment of the requirements ... 2007 Author: Wong Man Chui Title: Applications of Malliavin Calculus and White Noise Analysis in Interest Rate Markets, and Convertible Bonds with and without Symmetric Information Department:...
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Analysis and detection of human emotion and stress from speech signals
... fi center frequency of i subband bi bandwidth of i subband α logarithmic growth factor C bandwidth of first filter lm lower edge of m th filter bank hm upper edge of m th filter bank St ( m ) ... Kyi Tar Nwe and other family members for their support, understanding and encouragement i Table of Contents Acknowledgements i Table of Contents ii Summary vi List of Symbols viii List of Figures ... Motivation of This Research 1.5 System Overview 1.6 Purpose and Contribution of This Thesis 10 1.7 Organization of Thesis 11 Chapter 2: Review of Acoustic Characteristics and Classification Systems of...
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Genotoxicity of 255 chemicals in the Salmonella microsome test (Ames test) and 8-hydroxyguanine (8-OH-Gua) assay for the detection of carcinogens
... Kasai, H., Sofuni, T and Nohmi, T (1997) Construction of mutants of Salmonella typhimurium deficient in 8-hydroxyguanine DNA glycosylase and their sensitivities to oxidative mutagens and nitro ... 233-246 Yamada, M., Sedgwick, B., Sofuni, T and Nohni T (1995) Construction and characterization of mutants of Salmonella typhimurium deficient in DNA repair of O6-methylguanine, J Bacteriology, ... 2-Nitrofluoranthene, 1-nitropyrene and 1,8-dinitropyrene are tested without S9mix, Glu-P-1 and Trp-P-2 are tested with S9mix using TA98 and YG1041 2-Nitronaphthalene, 2,4-dinitrotoluen and 4-nitroquinoline...
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Detection of bacteria and enteric viruses from river and estuarine sediment
... ISOIL for Beads Beating (Nippon Gene, Tokyo) Detection of pathogens and fecal indicators by PCR and qPCR methods The concentration of (c)DNA of Poliovirus and Adenovirus was determined using real-time ... (1996) and Watson and Blackwell (2000) reported The color of supernatant after vortex mixing and centrifugation was brown and that of the concentrate after membrane filtration was yellow, both of ... composition of silt and clay The low recovery rate was also because of the inhibition of nucleic acids extraction and PCR by humic substances extracted from sediment by high pH as Tsai and Olson...
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Detection of Antiviral Drugs Oseltamivir Phosphate and Oseltamivir Carboxylate in Neya River, Osaka, Japan
... Quantifications of OP, OC and OC-D3 were based on calibration curves from to 1000 ng/L The limit of detection (LOD) and limit of quantification (LOQ) values for OP, OC and OC-D3 in this investigation ... development of oseltamivir-resistant influenza viruses Furthermore, Japan is at the top of the list of countries for per capita consumption of Oseltamivir (Soderstrom et al., 2009), and it has ... influenza and H1N1 influenza pandemic (September 2009 to February 2010) MATERIALS AND METHODS Chemicals and Reagents Oseltamivir phosphate (CAS-RN: 204255-11-8) was obtained from F Hoffmann-La...
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Tài liệu The Early Detection Research Network: Investing in Translational Research on Biomarkers of Early Cancer and Cancer Risk docx
... time -of- flight secondary ion mass spectrometer (TOF-SIMS), capable of chemical mapping of breast cells and tumor tissues Breast and Gynecologic Cancers 29 Ovarian Cancer Early detection of ovarian ... studies GP73 and its fucosylated glycoform, are proving to outperform the standard of care, alpha-fetoprotein, in detection of early stage cancer This represents an important proof of concept as ... achievements include: • tandard reference specimens and reagents, S primarily plasma and serum (cases and matched controls) were developed for detection and evaluation of prostate cancer biomarkers;...
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Tìm thêm: hệ việt nam nhật bản và sức hấp dẫn của tiếng nhật tại việt nam xác định các mục tiêu của chương trình xác định các nguyên tắc biên soạn khảo sát các chuẩn giảng dạy tiếng nhật từ góc độ lí thuyết và thực tiễn khảo sát chương trình đào tạo của các đơn vị đào tạo tại nhật bản xác định thời lượng học về mặt lí thuyết và thực tế tiến hành xây dựng chương trình đào tạo dành cho đối tượng không chuyên ngữ tại việt nam điều tra đối với đối tượng giảng viên và đối tượng quản lí khảo sát thực tế giảng dạy tiếng nhật không chuyên ngữ tại việt nam khảo sát các chương trình đào tạo theo những bộ giáo trình tiêu biểu nội dung cụ thể cho từng kĩ năng ở từng cấp độ phát huy những thành tựu công nghệ mới nhất được áp dụng vào công tác dạy và học ngoại ngữ các đặc tính của động cơ điện không đồng bộ hệ số công suất cosp fi p2 đặc tuyến hiệu suất h fi p2 đặc tuyến mômen quay m fi p2 đặc tuyến dòng điện stato i1 fi p2 động cơ điện không đồng bộ một pha phần 3 giới thiệu nguyên liệu từ bảng 3 1 ta thấy ngoài hai thành phần chủ yếu và chiếm tỷ lệ cao nhất là tinh bột và cacbonhydrat trong hạt gạo tẻ còn chứa đường cellulose hemicellulose