detection of bordetella pertussis by pcr

... by hydrophobic chromatography increased the specific activity of AC eluted with 0.1 M NaCl by a factor of 1.3 (from 138 UÆmg)1 to 185 UÆmg)1) and the percentage of CaM activation by a factor of ... residues of the catalytic site, favors a new activation mechanism Recently, the resolution of the 3D structure of the AC exotoxin of B anthracis and of its complex with CaM led to the proposal of a ... a direct exploitation of the structural results of the former to explain the mechanism of CaM activation of the latter Crystallization and resolution of the 3D structures of AC and AC–CaM, which...

Ngày tải lên: 07/03/2014, 15:20

... Number of Number of Number Number of Number Number of Number Results r of mice cleared mice cleared of of mice mice cleared of of mice mice cleared of of mice of monitoring mice of trypanosomes ... Number of Number Number of Number Number of Number Results r of mice cleared mice cleared of of mice mice cleared of of mice mice cleared of of mice of monitoring mice of trypanosomes killed trypanosomes ... Number of Number Number of Number Number of Number Results r of mice cleared mice cleared of of mice mice cleared of of mice mice cleared of of mice of monitoring mice of trypanosomes killed trypanosomes...

Ngày tải lên: 28/04/2014, 13:08

... Recognition of a bacterial adhesin by an integrin: macrophages CR3 (αMβ2, CD11b/CD18) binds filamentous hemagglutinin of Bordetella pertussis Cell 1990, 61, Mac-1-mediated Uptake and Killing of Bordetella ... enhanced by serum However, the increased binding and uptake of B bronchiseptica by addition of serum was not inhibited by anti-Mac-1 antibodies, indicating that the dramatic increase of B bronchiseptica ... of local opsonization of bacteria by complement components (C3bi) even in the absence of serum, as shown in Leishmania [30] and zymosan [7] Compared to porcine isolates, the type strain Bb5 of...

Ngày tải lên: 07/08/2014, 17:22

... ! ROLE OF THE CAPSULE LOCUS IN THE VIRULENCE OF BORDETELLA PERTUSSIS REGINA HOO MAY LING (B Sc (Hons), NUS) A THESIS SUBMITTED FOR THE DEGREE OF DOCTOR OF PHILOSOPHY DEPARTMENT OF MICROBIOLOGY ... 108! ! xiv! ! Figure 3.5: Detection of the polysaccharide capsule at the surface of B pertussis strains 111! Figure 3.6: Lung colonization profile by B pertussis BPSM, ΔkpsT, ΔkpsE ... Adenosine triphosphate B bronchiseptica Bordetella bronchiseptica B holmesii B holmesii B parapertussis Bordetella parapertussis B pertussis Bordetella pertussis BCIP 5-bromo-4-chloro-3'-indolyphosphate...

Ngày tải lên: 10/09/2015, 09:29

... 169 Figure 5.17 Selection of truncated NA by DNASTAR software 172 Figure 5.18 Detection of ∆NA in recombinant B pertussis 173 Figure 5.19 Detection of anti- ΔNA local and systemic ... BORDETELLA PERTUSSIS MICROBIOLOGY 53 2.8.1 Bordetella pertussis Pathogenesis and Whooping Cough 53 2.8.2 Treatment and Pertussis Vaccines 54 2.8.3 Virulence Determinants of ... analysis of M2e expressing recombinant B pertussis 139 Figure 5.2 Lung colonization profiles of M2e expressing B pertussis strains 141 Figure 5.3 In vitro growth kinetic of M2e expressing...

Ngày tải lên: 11/09/2015, 10:00

... and C value of SVM during optimization 71 vii List of Tables LIST OF TABLES Table 4.1 Function of components of limbic system 34 Table 4.2 The conductivity (S/m) of tissues below ... of independent components fall into both sides of the anterior temporal, clear correlation between the number of independent components on both sides of the anterior temporal and the status of ... my parents i Table of Contents TABLE of CONTENTS ACKNOWLEDGEMENT I TABLE OF CONTENTS II SUMMARY .IV LIST OF FIGURES VI LIST OF TABLES ...

Ngày tải lên: 22/10/2015, 21:19

... DNA fragment on PCR reaction PCR mixture (50 µl) contained µl of 10
PCR buffer, µl of 25 mM MgCl2, µl of 10 mM deoxynucleotide triphosphate mixture, 20 pmol of each primers, µl of DNA template, ... of PCR amplification DNA isolated from the intestinal specimens of pigs with PPE as well as DNA from several other bacterial strains, Fig Specificity of one-step PCR assay for the detection of ... genomic DNA were prepared by 10-fold serial dilutions from µg to 100 pg and subjected to PCR reaction to determine the sensitivity of the PCR assay The detection limit of the PCR with 35 cycles was...

Ngày tải lên: 07/08/2014, 14:22

... resulted in the presence of Ct value in real-time PCR detection The detection limit of the conventional PCR was determined through the highest dilution that resulted in the presence of clear amplified ... standard curve (Figure 1.b.) of the TaqMan™ FQ -PCR were generated by using the 10-fold dilutions of pcDNA3.1-gC, which has already known its copies to undertake FQ- Page of 10 PCR reaction under optimum ... http://www.virologyj.com/content/7/1/37 Page of 10 Figure The sensitivity of TaqMan™ FQ -PCR detection Ten-fold serial dilutions of AHV-1 standard template were used (1-6), 1.0 × 105-1.0 × 100 copies/reaction of AHV-1 standard...

Ngày tải lên: 12/08/2014, 04:21

... Comparisons of Clostridium chauvoei detected by culture followed by biochemical identification culture followed by PCR and direct PCR on muscle tissue from clinical samples of blackleg Page of (page ... Comparisons of Clostridium chauvoei detected by different methods Sample Material Culture followed by biochemical identification PCR on culture colonies PCR on muscle piece or blood PCR on meat juice PCR ... the PCR In most clinical cases of blackleg the amount of C chauvoei in infected tissue is high and can easily be detected by the PCR method independent of contaminating flora Moreover, the PCR...

Ngày tải lên: 12/08/2014, 18:22

... 25 lL PCR reaction, either 100 pg of DENV-1-, ng of DENV-2-, 100 pg of DENV3- or ng of DENV-4-infected C6 ⁄ 36 cells total RNA was used for cocktail PCR The primer cocktail was composed of 100 ... and presence of 1000-fold human DNA PCR detection of nearly any dengue virus strain Fig S7 Real-time PCR amplification curve of DENV1 (Piura, Peru) RNA in the absence and presence of 100-fold excess ... existence of multiple amplicons may enhance resistance to false negatives produced by escape mutants of these mutable RNA viruses e -PCR- based dengue virus detection sensitivity Amplification by the...

Ngày tải lên: 14/02/2014, 19:20

... the recall of DL1 Because a of DL1 is the same as that of DL0, the number of problems detected as errors by a is given by Eq.4 In the case of DL1, problems judged by ~ of DL0 are judged by the written ... the number of problems detected as errors by DL1 is given by Eq.4, and the number of real errors in these detections is TGpg Therefore, the precision P1 of DL1 is as follows: p1 = Pg by a is 1,631, ... be the number of homophone problems for a homophone set We solve them by the original decision list D L O Let G (or H ) be the ratio of the number of homophone problems by judged by a (or f~ )...

Ngày tải lên: 22/02/2014, 03:20

... pH-3c digital A solution of 40.0 mL of dimethylformamide in 40.0 mL of methylene chloride was chilled in an ice bath A solution of 37.0 mL of phosphorus oxychloride in 35.0 mL of methylene chloride ... conditions for the analysis of O2–Æ, the effect of buffer solution and the concentration of the fluorescent probe were investigated Effect of pH and buffer concentration The pH of the medium has a large ... created by the enzymatic reaction of FEBS Journal 274 (2007) 1725–1733 ª 2007 The Authors Journal compilation ª 2007 FEBS 1727 Selective detection of superoxide anion radicals J J Gao et al Effect of...

Ngày tải lên: 16/03/2014, 11:20

... lamellae by sintering was inhibited by adding nano-particles of SnO2 However the amount of addition of SnO2 nano-particles seems to have the most suitable value The sensor response, when the amount of ... prepared by the acidification of NaWO4 with a strong acid solution (H2SO4 at pH = -0.8) [5] On the other hand, sol of crystalline SnO2 with mean grain (crystallite) size of nm was prepared by hydrothermal ... concentration range by improving the sensor response at high NO2 concentration, we investigated the microstructure control of WO3 film by adding nanoparticles of SnO2 Experimental Sol of WO3*2H2O with...

Ngày tải lên: 20/03/2014, 13:04

... hydrolysis of each of the GD stereoisomers by wild-type HuPON1 differs by less than one order of magnitude (Table 1) The kcat values of the individual isomers are quite similar, with the turnover of ... determination of Km, kcat, and kcat ⁄ Km values of HuPON1 for each GD stereoisomer, resulting in unambiguous elucidation of the extent of stereoselectivity of HuPON1-mediated hydrolysis of GD 1184 ... 1.67 mm racemic GD by HuPON1 Fig S7 Eadie–Hofstee plot of theoretical solutions and measured data for hydrolysis of 1.67 mm racemic GD by HuPON1 This material is available as part of the online article...

Ngày tải lên: 30/03/2014, 09:20

... smaller list of NPIs as a seed set Begin with a small set of seed NPIs Iterate: (a) Use the current list of NPIs to learn a list of DEOs (b) Use the current list of DEOs to learn a list of NPIs Interestingly, ... as a generalization of this evaluation procedure that is sensitive to the ranking of DEOs and non-DEOs For development purposes, we use the list of 150 annotations by DLD09 Of these, 90 were DEOs, ... to the distillation method of DLD09 In particular, the numerator of (17), which we just showed to be equal to the estimate of P (Y ) given by (16), is exactly the sum of the responsibilities for...

Ngày tải lên: 31/03/2014, 20:20

... Relief of inhibition by meat, blood, and feces Relief of inhibition by bilirubin and humics Relief of inhibition by melanin in RT -PCR Relief of inhibition by meat, blood, and feces Relief of inhibition ... inhibition by bilirubin and humics Rescue of failed amplification Facilitation of RT -PCR Relief of inhibition by feces Relief of inhibition by plant polysaccharides Relief of inhibition by blood ... Pre -PCR processing comprises all steps prior to the detection of PCR products Thus, prePCR processing includes the composition of the reaction mixture of PCR and, in particular, the choice of...

Ngày tải lên: 11/04/2014, 10:01

... also Page of 25 (page number not for citation purposes) Journal of Translational Medicine 2008, 6:61 serve as antigen presenting cells (APC) in real time RTPCR and ELISPOT analysis Proof of principle ... insufficient number of cells Accuracy The accuracy of an analytical method describes the closeness of mean test results (detected) obtained by the method to the true value (expected) of the analyte ... Precision The precision of an analytical method describes the closeness of agreement (degree of scatter) between a series of measurements obtained from multiple sampling of the same homogenous...

Ngày tải lên: 18/06/2014, 15:20

... The performance of the previous detectors are computed in terms of Probability of Detection (PD) of the known useful signal as a function of either its input SNR or the Probability of False Alarm ... a frequency offset compensation, the vector of complex envelopes of the signals at the output of the sensors is a scaled, delayed, noisy and multidimensional version of s(t) given by xτ(t) = µs ... purpose of this paper is to introduce and to analyze the performance of different array receivers for the detection of a known signal, with different sets of unknown parameters, corrupted by an...

Ngày tải lên: 21/06/2014, 00:20

... molecular size-selective detection method for a protein in the presence of its aggregate by utilizing a heteroliganded gold nanoisland on a transparent glass substrate As a proofof-concept test, the ... range of 1.0 to 0.1 mg/ml Figure 3A shows representative Optimization of heterolihanded gold nanoisland for the high sensitivity For the sensitive detection of a protein, the thickness of the ... concentration of the protein than other metallic nanoparticle systems [38,39] Size-selective detection of SOD1 in the presence of its aggregate Figure (A) Representative absorption spectra of SOD1...

Ngày tải lên: 21/06/2014, 04:20