detection and analysis of dna methylation by pyrosequencing

detection and analysis of genetic alterations

Ngày tải lên : 10/04/2014, 22:12

... metastasis samples were analysed by the standard approach of single strand conformation polymorphism analysis (SSCP) /DNA sequencing and by this pyrosequencing strategy Pyrosequencing was found to ... more intricate, and thus more easily influenced folding of the strands (Highsmith, 1999) Detection and analysis of genetic alterations in normal skin and skin tumours The detection of fragments ... Principles of methods used for detection of mutations/variations at defined positions 15 Detection and analysis of genetic alterations in normal skin and skin tumours Pyrosequencing The principle of...

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Báo cáo y học: "Detection and analysis of alternative splicing in Yarrowia lipolytica reveal structural constraints facilitating nonsense-mediated decay of intron-retaining transcripts" ppt

Ngày tải lên : 09/08/2014, 20:22

... 8,614 and 8,172 clones in the expo, stat and oleic libraries, respectively About 59% of the predicted genes (3,818 of 6,449) were expressed and found in at least one library and about 70% of these ... one and three (Figure 2b), potentially accounting for the observed bias in favor of phase and phase introns Second, introns of size 3n were underrepresented (29.4% of all introns versus 35.5% and ... multiples of nucleotides (blue line), multiples of plus nucleotides (orange line), and multiples of plus nucleotides (green line) For each class, the number of introns is reported as a function of size,...

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Báo cáo y học: "A comparative analysis of DNA methylation across human embryonic stem cell lines" docx

Ngày tải lên : 09/08/2014, 23:20

... conservation and variability of DNA methylation across different stem cell lines A recent analysis of about 1% of the genome of HESC lines shows that, by monitoring DNA methylation and gene expression, ... supported by an Innovation Award from the Eli and Edythe Broad Center of Regenerative Medicine and Stem Cell Research at UCLA (to SEJ and MP), and by the Department of Energy Office of Science ... (low) methylation Conserved Discordant methylation Unconserved Methylation Group Conser ved methylated Unconser ved met hylated Unmethylated Figure Conservation and DNA methylation of CG and non-CG...

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Báo cáo y học: " Genome-wide detection and analysis of homologous recombination among sequenced strains of Escherichia coli" pot

Ngày tải lên : 14/08/2014, 16:21

... appendix of Additional data file that this is indeed the case for these six genomes Random SNDs can and form clusters of identical patterns simply by chance Given the number of SNDs and their ... Seillier-Moiseiwitsch F: Stepwise detection of recombination breakpoints in sequence alignments Bioinformatics 2005, 21:589-595 Stephens JC: Statistical methods of DNA sequence analysis: detection of intragenic ... 7.5% of the sequence alignment A breakdown by pattern and range of scores is arrayed in Tables and reviews Here again, BLAST statistics [39] serve as the model for assessing significance Random...

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detection and analysis of musical events using model-based signal processing

Ngày tải lên : 29/11/2014, 06:59

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Tài liệu Báo cáo khoa học: The stereochemistry of benzo[a]pyrene-2¢-deoxyguanosine adducts affects DNA methylation by SssI and HhaI DNA methyltransferases pptx

Ngày tải lên : 19/02/2014, 00:20

... concentrations of B[a]P-N2-dG adducts were compared with the levels of DNA methylation, and thus the effect of the absolute conﬁgurations and conformations of the adducts on DNA methylation was ... kinetics of methylation of oligodeoxynucleotide duplexes containing (+)-cis-B[a]P-N2-dG adduct by M.SssI and M.HhaI The rates of methylation of the X+CG ⁄ CGC, GCX+ ⁄ CGC, X+CG ⁄ CGM and GCX+ ... fraction of bound 32P-labeled DNA (R) is the ratio of the fraction of bound 32 P-labeled DNA in the presence of the competitor DNA (cpmbound ⁄ cpmtotal) to the fraction of bound 32P-labeled DNA in...

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báo cáo khoa học: " Identification and analysis of common bean (Phaseolus vulgaris L.) transcriptomes by massively parallel pyrosequencing" doc

Ngày tải lên : 11/08/2014, 11:21

... and to the writing of the manuscript ZL helped with experimental verification, analysis of sequence data, and contributed to writing of the manuscript BM helped with conceiving and analysis of ... genomic DNA and cDNA; no amplification from cDNA was observed Lane 1, 100 bp ladder; Lane 2, genomic DNA; Lane 3, leaf cDNA; Lane Negative cDNA control (no reverse transcriptase was added to cDNA ... library preparation beads and ss-cDNAs were captured Number of molecules of the ss-cDNAs was calculated using the concentration and average fragment length emPCR, Enrichment and DNA Bead Loading Emulsion...

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Báo cáo y học: " Genital herpes evaluation by quantitative TaqMan PCR: correlating single detection and quantity of HSV-2 DNA in cervicovaginal lavage fluids with cross-sectional and longitudinal clinical data" doc

Ngày tải lên : 12/08/2014, 02:20

... (slope of standard curve), linearity (R-square) and reproducibility (mean threshold (Ct) values, standard deviation (SD) and coefficient of variation (CV)) from standard curve data Limit of detection ... reported history of genital herpes sores and presence of herpetic lesions Page of PCR assay adapted two previously reported methods [28,29] used for detection and typing of HSV DNA in mucocutaneous ... median visit number of (IQR: 1-8) Methods Study population and specimens Extraction of HSV DNA The study population consisted of HIV infected and uninfected participants of Women’s Interagency...

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An analysis of errors made by vietnamese secondary school students in using english modal auxiliary verbs can, could, may, must and semi auxiliary verb have to

Ngày tải lên : 18/12/2013, 10:04

... speakers often not only qualify their commitment to assertion but also soften and hedge their judgments and orders and strengthen the expressions of their feelings and opinions of their feelings and ... the inappropriate use of language in the term of social acceptability or language performance 1.5.3 Error analysis Error analysis is the study and analysis of the errors made by second or foreign ... includes the description of research site, participants, and data collection techniques and data analysis methods Then, the results and findings of the study will be presented and discussed - Chapter...

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Tài liệu Báo cáo khoa học: Kinetics and thermodynamics of nick sealing by T4 DNA ligase pptx

Ngày tải lên : 21/02/2014, 00:20

... ligase catalysis Joining of nicked dsDNA by T4 DNA ligase involves three catalytic steps: formation of the enzymeadenylate, formation of the ndsDNAadenylate, and sealing of the nick These processes ... Biphasic kinetics of joining of 3Â-oligonucleotides (M5C19, C24, M1C6, and M4C7) to 72/24-mer BC [dsDNA] was lM; [ATP] was 5.6 lM for M5C19, C24, and mM for M1C6 and M4C7 Ligation of M5C19 and C24 was ... M4C7 [dsDNA] (1 lM) corresponds to the 125 turnovers of T4 DNA ligase in the case of C24 joining, 10 turnovers in the case of M5C19, and 2.5 turnovers for M1C6 and M4C7 4318 A V Cherepanov and S...

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Báo cáo khoa học: Photoregulation of DNA transcription by using photoresponsive T7 promoters and clariﬁcation of its mechanism doc

Ngày tải lên : 15/03/2014, 10:20

... reversibly photoisomerized between trans and cis forms [23–25] By photoswitching DNA hybridization, DNA primer extension by DNA polymerase and RNA digestion by RNase H can be successfully photoregulated ... instead of Azo (Fig 1) Clear-cut on–off photoregulation of DNA transcription was obtained because of the efﬁcient suppression of T7 RNA polymerase (RNAP) binding to the DM-azo in the trans form By ... with [32P]ATP[aP]) and the photoregulatory efﬁciency of transcription (a) was Fig Sequences of photoresponsive T7 promoters and the structures of Azo and its derivatives (M-azo and DM-azo) used...

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Báo cáo khoa học: Recognition of DNA modiﬁed by trans-[PtCl2NH3(4hydroxymethylpyridine)] by tumor suppressor protein p53 and character of DNA adducts of this cytotoxic complex potx

Ngày tải lên : 16/03/2014, 14:20

... facilitates sample analysis Hence, it is possible to prepare easily and precisely the samples of DNA modified by the platinum complex at a preselected value of rb The samples of DNA modified by new platinum ... different amount of DNA adducts of transplatin and its analogue removed from DNA by thiourea is not due to a different efficiency of thiourea to displace the monofunctional adducts of these different ... (nonmodified) DNA; 1, rb ¼ 0.01; 2, rb ¼ 0.03; 4, rb ¼ 0.05 (B) Changes in the CD spectra of DNA at the maximum of the positive band (280 nm) in the CD spectra of DNA modified by cisplatin and clinically...

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Báo cáo khoa học: Substrate recognition and ®delity of strand joining by an archaeal DNA ligase docx

Ngày tải lên : 17/03/2014, 11:20

... L -DNA( 40), 10 lM R -DNA( 30), lM T -DNA( 80) and 200 nM LigTk, with (right side) or without (left side) excess duplex DNA The duplex DNA was a mixture of 10 lM of 50-mer DNA- A and 10 lM of 50-mer DNA- B ... ATP, and 50 nM LigTk DNA substrate [L -DNA( 40), R -DNA( 30), and T -DNA( 80)] were added at various concentrations in the range 0.5±4 lM With radiolabelled DNA substrates, 0.1 lM L -DNA, 0.1 lM T -DNA and ... (L -DNA and R -DNA) and a complementary oligonucleotide (T -DNA) A phosphate group was present at the 5¢ terminus of R -DNA Deletions, insertions and mutations were introduced to the L -DNA( 40), R -DNA( 30)...

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Báo cáo Y học: Thermodynamics and kinetics of the cleavage of DNA catalyzed by bleomycin A5 A microcalorimetric study pdf

Ngày tải lên : 24/03/2014, 04:21

... nicking of pBR-322 DNA by a mixture of ADM, Fe3+, Vc and O2 and by a mixture of (1,10-phenanthroline)-copper(II), ME and O2 converted pBR-322 DNA to small DNA fragments [39] and linear DNA [31], ... on DNA binding, compartment I of the reaction cell contained mL of a DNA solution and compartment II of the reaction cell contained mL of a BLM-A5 solution The heat released by dilution of DNA ... interactions of DNA with many small molecules, such as BLM and ADM, are at rapid equilibrium: DNA þ L Ð DNA Á L ð13Þ where L is a small molecule that binds to DNA and DNA L the complex between DNA and...

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Báo cáo khoa học: The thermodynamic analysis of protein stabilization by sucrose and glycerol against pressure-induced unfolding The typical example of the 33-kDa protein from spinach photosystem II docx

Ngày tải lên : 31/03/2014, 01:20

... Plot of the standard volume change of the 33-kDa protein in the presence of diﬀerent concentration of NaCl (d) and sucrose (m) vs the reciprocal of the sucrose osmolarity In Fig 8, the standard ... Kinetics of the pressure-induced unfolding and refolding of the 33-kDa protein in the presence of 30% glycerol Other conditions as in Fig Table Relaxation time of unfolding and refolding of the ... denaturation Effects of sucrose and glycerol on the kinetics of the pressure induced protein unfolding and refolding The unfolding and refolding kinetics in the presence of either 0.83 M sucrose...

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Báo cáo Y học: The processivity and ﬁdelity of DNA synthesis exhibited by the reverse transcriptase of bovine leukemia virus pot

Ngày tải lên : 31/03/2014, 21:21

... potent inhibitors of other RTs such as that of HIV [12] In the present study we have extended the investigation of BLV RT by an in vitro analysis of the processivity and the ﬁdelity of DNA synthesis ... ability of BLV RT to misincorporate nucleotides at the 3¢ end of the growing DNA strand and the further extending of the preformed mismatches) MATERIALS AND METHODS Recombinant RTs and DNA polymerase ... (depending on activity) and Ó FEBS 2002 Processivity and ﬁdelity of BLV RT (Eur J Biochem 269) 861 Table Quantitative analysis of DNA primer-extension and relative processivity of DNA synthesis The...

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evaluation and comparison of management strategies by data envelopment analysis with an application to mutual funds

Ngày tải lên : 03/06/2014, 01:55

... quote Chandler, a pioneer of the field: strategy is “…the determination of the basic long-term goals and objectives of an enterprise, and the adoption of courses of action and the allocation of resources ... elaboration of the classes of decisions made by managers, the explicit separation of strategy from goals and objectives, and the concept of risk as a function of the amount and extent of knowledge, ... evaluation of each separate aspect of performance in this industry is greatly facilitated by the fact of publicly declared and legally binding strategies for its firms and by the public reporting of...

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báo cáo hóa học: " Use of alcohol and drugs by Norwegian employees: a pilot study using questionnaires and analysis of oral fluid" doc

Ngày tải lên : 20/06/2014, 00:20

... comparison of men and women, different age groups, or different recruitment procedures Results of questionnaire and analysis of oral fluid The principal results of the questionnaire and analysis of oral ... oral fluid EtG and drugs were analysed by liquid chromatography - tandem mass spectroscopy [28,31] The compounds analysed and the cut-off concentrations (in the mixture of oral fluid and buffer) ... product and metabolite of heroin, only illegal use in Norway 0.41 7-aminoclonazepam Degradation product and metabolite of clonazepam 0.36 7-aminoflunitrazepam Degradation product and metabolite of...

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Báo cáo hóa học: " Genetic analysis of hantaviruses carried by Myodes and Microtus rodents in Buryatia" doc

Ngày tải lên : 20/06/2014, 01:20

... supported by grants from the University of Helsinki, The Academy of Finland, Sigrid Jusélius foundation (Finland) and EU grant GOCE-CT-2003-010284 EDEN and the paper is officially catalogued by the ... sequences of the N protein of PUUV, HOKV, and MUJV were of the same length (433 aa residues), a clear sign of their close genetic ties and shared evolution Furthermore, the N protein sequence of Muhorshibir767 ... samples, performed RT-PCR and sequencing, participated in the genetic analysis and contributed to writing of the manuscript JL, JN and BP participated in fieldwork and in Page of (page number not for...

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Báo cáo hóa học: "Research Article Detection and Correction of Under-/Overexposed Optical Soundtracks by Coupling " pot

Ngày tải lên : 22/06/2014, 01:20

... 4.1 Distortion detection by center of gravity shifts The center of gravity of a spectrum (COG) is in a sense, the “mean” frequency, and this method is used for pitch detection and for audio restoration ... original image and its opening (or closing) in function of the size of structuring elements A divergence between the graph of openings and the one of closings means that the surface of the peaks ... transcript of the 2D VA modulation, a morphological study of the 1D signal shape will of course make sense, using, for instance, morphological operators or analysis of local derivatives of the signal...

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