detecting gene protein expression and signaling

... of proteins Captured proteins are then analyzed by TOF-MS, generating a spectral map depicting approximations of the molecular weight (m/z) and relative concentration (intensity) of each protein ... cancer patients and healthy volunteers was collected and analyzed A panel of differentially expressed proteins was advocated for biomarkers of diagnosis for colorectal cancer Materials and methods ... mixture was aspirated and spotted onto the gold-coated ProteinChip array After air-drying for minutes at room temperature, protein crystals on the chip were scanned with the ProteinChip (Model PBS...

Ngày tải lên: 25/10/2012, 11:18

... IPTG; lane 0, protein standards (C) Protein bands after affinity chromatography and renaturing process Lanes and 2, protein bands after separation by affinity column; lanes and 4, protein bands after ... fusion protein was renatured and examined by SDS ⁄ PAGE gel (Fig 5C), indicating a clear and unique protein band of 21.7 kDa, which matched well with the theoretical mass of the fusion protein ... Cc-CATH2 expression and purification In the Escherichia coli BL21 and pET-32a(+) plasmid protein expression system, the deduced mature CcCATH2 was expressed directly as a His-tagged fusion protein...

Ngày tải lên: 28/03/2014, 23:20

... hsa-mir-29a and b inhibited Nef expression and HIV-1 replication hsa-mir-29a and b inhibited Nef expression and HIV-1 replication (A) Nef expression was inhibited by hsa-miR-29a and 29b HEK293T ... hsa-mir-29a and 29b pEGFP-miRNA construct expressing hsa-mir-29a and 29b (A) Diagrammatic representation of pEGFP-N3 vector containing pre-miR29a and 29b (B) Expression of hsa-mir-29a and 29b confirmed ... 324–5p and 378) that were prioritized from the predictions by miRanda[11] and further refined using RNAhybrid[12], MicroInspector[13], and DIANAMicroT[14], showed putative targets in four HIV-1 genes...

Ngày tải lên: 13/08/2014, 05:21

... of HOX genes, which are key players in embryogenesis and development Because of their importance in gene regulation and disease, MLLs have been isolated from human cells and their protein protein ... and target gene expression MLLs are critical for HOX gene regulation and embryonic development Homeobox genes are a group of evolutionarily conserved genes that encode transcription factors and ... regulatory genes, MLL1 plays important roles in the regulation of stressresponsive genes MLL3 and MLL4 act as a p53 coactivator (a tumor suppressor gene) and are required for H3K4 trimethylation and expression...

Ngày tải lên: 16/02/2014, 14:20

... activation and NAMI-A- induced down regulation of c-myc gene expression NAMI-A inhibits c-myc gene transcription and protein expression To establish whether the decrease in c-myc mRNA expression ... regulates c-myc gene expression To investigate whether NAMI-A-induced inhibition of ERK1/2 signaling could affect the expression of a cell growth-related gene, we assessed c-myc gene expression in ... FEBS 2002 NAMI-A affects c-myc gene expression and MEK/ERK phosphorylation (Eur J Biochem 269) 5867 Fig NAMI-A and PD98059 completely inhibits PMA-induced c-myc gene expression (A) Serum-starved...

Ngày tải lên: 21/02/2014, 01:21

... organization and chromosomal location Eur J Biochem 173, 35–44 27 Andre, M., Ando, S., Ballagny, C., Durliat, M., Poupard, G., Briancon, C & Babon, P.J (2000) Intestinal fatty acid binding protein gene expression ... The mouse intestinal fatty acid binding protein gene: nucleotide sequence, pattern of developmental and regional expression, and proposed structure of its protein product DNA Cell Biol 11, 31–41 ... other species, and not to CRBPI or other intracellular lipid-binding proteins from zebrafish (Fig 2), the duplication of the ancestral CRBP gene that gave rise to the CRBPI and CRBPII genes most likely...

Ngày tải lên: 17/03/2014, 11:20

... lipid-binding proteins and their genes Annu Rev Nutr 17, 277–303 25 Wu, Q., Andolfatto, P & Haunerland, N.H (2001) Cloning and sequence of the gene encoding the muscle fatty acid binding protein from ... between zebrafish and human, mouse and pufferfish B-FABP is 83%, 76% and 83%, respectively The percentage amino acid identity between zebrafish and human and mouse is higher in the exons and than it ... homeodomain proteins in the brain [32 and references therein] and at least some of the homeobox genes or homeodomain proteins are required for neuronal development [35,36] In a recent morphological and...

Ngày tải lên: 31/03/2014, 07:20

... proinflammatory gene expression in C/EBPb-/- mixed glial cultures Expression of pro-inflammatory (NOS2, IL-1b, IL-6 and TNFa) and anti-inflammatory (IL-4 and TGFb1) genes in C/EBPb+/+ (white bars) and C/EBPb-/- ... (n = 4-5) NOS2 protein levels by western blot and immunocytochemistry, and generation of NO by colorimetric detection of nitrites (Griess assay) In wild-type cultures NOS2 protein expression was ... as the ratio between the intensity of the protein of interest band and the loading control protein band (b-actin) Quantitative real time PCR (qPCR) mRNA expression was determined in mouse mixed...

Ngày tải lên: 19/06/2014, 22:20

... both MMP-13 mRNA expression and the protein to an approximately similar extent Cathepsin K gene expression and protein synthesis The levels of both the gene expression and the protein of cathepsin ... correlation exists between the mRNA and protein levels At the two dosages tested, the R1094 ADAMTS-4 and ADAMTS-5 gene expression and protein synthesis The level of gene expression of ADAMTS-5 in OA ... mechanisms of ADAMTS-4 and ADAMTS-5 gene expression and protein synthesis are complex and may vary based on species and culture conditions [15] The present study found the level of expression of ADAMTS-5...

Ngày tải lên: 09/08/2014, 06:23

... transcribe and generate siRNA continuously and the gene silencing effect can last persistently inside the cells These findings have opened a broad new avenue for the analysis of gene function and gene ... MTA1 gene silence and consequentially recover the protein expression of ER alpha, resulting in increase sensitivity of antiestrogens, as well as suppress the protein expression of MMP-9 and cyclinD1 ... MDA-MB231, and protein levels of MMP-9 and CyclinD1 were down-regulation (P < 0.05) However, in ER alpha-positive breast cancer cells MCF-7, protein expression levels of ER alpha, MMP-9 and CyclinD1...

Ngày tải lên: 10/08/2014, 10:21

... using the EF1a gene as a reference [6,37] to detect the expression of 15 AtPP2-A genes and 15 AtPP2-B genes [23] As shown in Figure 3a, transcript levels of the genes, except AtPP2-A1 and AtPP2A14, ... bioinformatics analyses and determined gene expression HS also investigated aphid nymphs XW, LY and RL generated and characterized transgenic plants BL, Page 18 of 19 JQ, WS and ZY participated in ... basal expression and HrpNEa-induced expression of AtPP2-A14 (Figure 3c) The other eight mutants behaved differently in expression of the corresponding AtPP2-A genes AtPP2-A3, -A11, -A13, and -A15...

Ngày tải lên: 11/08/2014, 11:21

... 10 Protein Expression Level (AU) Figure mRNA and protein half-lives and induction time on the appearance of protein expression histograms Effect of5 Effect of mRNA and protein half-lives and ... Figure mRNA and protein Effect ofexpression protein half-lives on the dynamics of Effect of mRNA and protein half-lives on the dynamics of protein expression (A) Binary switching of a gene template ... observe binary, hybrid and graded protein expression Protein expression histograms of β-gal, Luc and GFP In examining different mode of gene induction, several reporter genes have been used To...

Ngày tải lên: 13/08/2014, 23:20

... (enzymes and transporters) Next, the proteins were assigned to a phyletic category: mammalian-specific proteins, metazoan-specific proteins, eukaryote-specific proteins and universal proteins ... mouse proteins (a) Expression pattern of all mapped proteins expressed in at least a single tissue (5,528 out of 6,242 proteins) (b) Expression pattern of proteins in different functional groups and ... and transporter proteins (b) The regulatory group includes signal transduction and transcription regulation proteins The pre-metazoa group includes universal proteins and eukaryote specific proteins...

Ngày tải lên: 14/08/2014, 14:21

... discussions and Edith Trejo and Carlos Zadikian for technical assistance We are indebted to Drs J J Cazzulo and C Labriola for their generous gifts of a cruzipaincontaining plasmid and primers A and ... [10] In the present Foreign ADC gene expression in T cruzi work we used ADC-transgenic protozoa to follow the time-course modulation of foreign gene expression and to investigate whether this ... the parasite genome organization Results and Discussion Expression of the oat ADC gene in T cruzi In order to study the expression of the foreign ADC gene in T cruzi epimastigotes, we transformed...

Ngày tải lên: 19/02/2014, 07:20

... specific for human Ras-GRF1 Primer pairs: lane 2, ON359 and ON360; lane 3, ON357 and ON358; and lane 4, ON361 and ON360 The PCR products and a DNA size marker, lane 1, were separated on agarose ... that the treatment of NIH3T3 and COS-7 cells with carbachol [9] and lysophosphatidic acid [11], activating both Gq- and Gi-coupled receptors, induces the activation and phosphorylation of Ras-GRF1 ... increase and show Ca2+ dependence of serotonininduced ERK1 ⁄ phosphorylation and a mandatory role of the Ca2+ ⁄ CaM-binding IQ domain in RasGRF1-stimulated ERK1 ⁄ phosphorylation Thus, both cAMP and...

Ngày tải lên: 19/02/2014, 18:20

... cytoplasmic protein X, and the protein X–Gc fusion protein and integral membrane protein Y in its native form were coexpressed in a ste18D strain The interaction between protein X–Gc and protein ... scientific and engineering perspective Ras signaling- based screening systems for protein protein interactions Small monomeric G -protein signaling in yeast Small monomeric G-proteins, such as Ras and ... signaling and is fused with various reporter genes associated with growth and photometry Auxotrophic or drug-resistant reporter genes, such as HIS3 or hph, are generally used for selection, and...

Ngày tải lên: 06/03/2014, 11:20

... catalytic cycle of the protein disulphide isomerases J Mol Biol 335, 283– 295 22 Lambert N & Freedman RB (1983) Kinetics and specificity of homogeneous protein disulphideisomerase in protein disulphide ... using various substrates and compared with those of hPDI The recombinant cPDI and hPDI shared similar reductase, oxidase and isomerase activities PDI exhibits both chaperone and antichaperone activities ... Cl, pH 7.5, mM EDTA) The filled and open circles denote native and linear tx3a, respectively (B) Refolding carried out in buffer B (buffer A plus mM GSH and mM GSSG) and in buffer C (buffer B plus...

Ngày tải lên: 07/03/2014, 05:20

... First, mammalian expression vectors for FLAG epitope-tagged CCN4 ⁄ WISP1, WISP1v and WISP1vx proteins were constructed, and the proteins were overexpressed by DNA transfection of these expression vectors ... Production of the WISP1v proteins in HCS-2 ⁄ cells and their overexpression (A) Molecular constructs for the overexpression of WISP1, WISP1v and WISP1vx A mammalian expression vector, pFLAG-CMV ... chondrosarcoma and it is one of the cell lines known to retain the characters of chondrocytes, as represented by the expression of type II and type X collagen genes To assess whether WISP1v and WISP1vx...

Ngày tải lên: 07/03/2014, 10:20

... [20,22] and Bacillus subtilis lipase A [23] Results and Discussion Cloning and expression of Rv1399c The Rv1399c gene product encodes a 318 amino acid protein with a molecular mass of  31.7 kDa and ... 100 lgÆmL)1 ampicillin and 34 lgÆmL)1 chloramphenicol until a D600 value between 0.6 and 0.8 was reached Protein expression was induced by adding mM IPTG for h at 37 °C and then 16 h at 15 °C ... composed of parallel b strands associated to an antiparallel strand (b2) and is surrounded by helices (a1, a2, a3, a7 and a8) The second domain consists of helices a4, a5 and a6 all clustered on...

Ngày tải lên: 07/03/2014, 16:20

... Regulatory gene i Transcription Promoter p Operator o 3′ Gene E Gene D Gene C Gene B Gene A Transcription is blocked RNA polymerase Repressor–corepressor complex (active) mRNA Translation Repressor protein ... promoter of genes that encode proteins Unlike prokaryotic genes, eukaryotic genes are not clustered in operons, and each is divided into introns and exons Transcription from the template strand proceeds ... on other genes Other important protooncogenes that encode nuclear transcription factors include MYC and MYB Both phytochrome (see Chap- Gene Expression and Signal Transduction ter 17) and gibberellin...

Ngày tải lên: 07/03/2014, 21:20