desorption ionization time of flight mass spectrometry
Ionization Methods in Organic Mass Spectrometry pot
... choice of ionization method depends on the nature of the sample and the type of information required from the analysis So-called 'soft ionization' methods such as field desorption and electrospray ionization ... analyzed by mass spectrometry Although ionization and desorption/ desolvation are usually separate processes, the term "ionization method" is commonly used to refer to both ionization and desorption ... pressure or reaction time, and nature of sample Mass range Low Typically less than 1,000 Da Desorption Chemical Ionization (DCI) Summary This is a variation on chemical ionization in which the...
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báo cáo khoa học: " Analysis of expressed sequence tags from a single wheat cultivar facilitates interpretation of tandem mass spectrometry data and discrimination of gamma gliadin proteins that may play different functional roles in flour" ppt
... electrospray ionization; EST: expressed sequence tag; HMW: high molecular weight; LMW: low molecular weight; MALDI-TOF: matrix-assisted laser desorption/ ionization time of flight mass spectrometry; ... D: Characterization of B- and C-type low molecular weight glutenin subunits by electrospray ionization mass spectrometry and matrix-assisted laser desorption/ ionization mass spectrometry Proteomics ... effects of mineral nutrients and high temperature Eur J Agron 2006, 25:96-107 Ferranti P, Mamone G, Picariello G, Addeo F: Mass spectrometry analysis of gliadins in celiac disease J Mass Spectrom...
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Tài liệu Real-time, on-line monitoring of organic chemical reactions using extractive electrospray ionization tandem mass spectrometry pdf
... which is carried out on a quadrupole time- of- flight (Q-TOF) mass spectrometer The relatively simple setup allows this method to be implemented on any type of MS instrument equipped with an ESI/APCI ... cone voltage was 40 V The Q-TOF mass spectrometer (QTOF UltimaTM, Micromass/Waters, Manchester, UK) was running in positive ion detection mode, Figure Schematic view of the EESI setup Copyright ... inlet of the mass spectrometer at a 408 angle from the axis of the sampling cone (shown in Fig 1) By introducing an intermittent, or if necessary continuous, N2 gas flow (50 L/h) through one neck of...
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Báo cáo khoa học: Determination of thioxylo-oligosaccharide binding to family 11 xylanases using electrospray ionization Fourier transform ion cyclotron resonance mass spectrometry and X-ray crystallography pot
... & Whitehouse CM (1989) Electrospray ionization for mass spectrometry of large biomolecules Science 246, 6471 52 Loo JA (2000) Electrospray ionization mass spectrometry: a technology for studying ... Int J Mass Spectrom 200, 175 186 53 Daniel JM, Friess SD, Rajagopalan S, Wendt S & Zenobi R (2002) Quantitative determination of noncovalent binding interactions using soft ionization mass spectrometry ... (1998) Fourier transform ion cyclotron resonance mass spectrometry: a primer Mass Spectrom Rev 17, 135 56 Smith RD (2000) Evolution of ESI -mass spectrometry and Fourier transform ion cyclotron...
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Real-time Monitoring technology of Mass spectrometry Speaker
... mass spectrometry for no-line trace gas analysis David Smith and Patrik Spanel Reference : Mass Spectrometry Reviews, 2005, 24, 661– 700 Application of IMS IMS/TOF IMS/IMS/TOF Ion Mobility/TOFMS ... J Mass Spectrom Ion Processes, 173 (1998) 191-241 J Mass Spectrom Ion Processes, 173 (1998) 191-241 J Mass Spectrom Ion Processes, 173 (1998) 191-241 PTR mass spectrometry Advantages: Soft ionization ... Outline IMS PTR-MS SIFT-MS Application of IMS IMS-TOF IMS-IMS-TOF Application of PTR-MS PIT-MS Real -time Monitoring Mass spectrometry Environmental science Biochemical and medical...
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báo cáo hóa học: " Method optimization and validation for the simultaneous determination of arachidonic acid metabolites in exhaled breath condensate by liquid chromatography-electrospray ionization tandem mass spectrometry" doc
... analytical run time, including the recondition step of the column for the next injection, was 21 Figure represents an example of a chromatogram of spiked EBC with each analyte Mass spectrometry ... more sources of errors The specificity of the MS detection ensures an unequivocal determination of the analysed substances Page of (page number not for citation purposes) Journal of Occupational ... quantitative retention of all analytes was achieved only by the ADS C18 Table 1: Program of time controlled steps for the LC gradient pump and the six-port switching valve Time (min) Flow (mL/min)...
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Determination of EDTA in Water Samples by SPE-Gas Chromatography/Mass Spectrometry
... at 1.2 mL/min Mass spectrometry was carried out using a 5973 Mass Selective Detector (Hewlett Packard, Wilmington, DE, USA) in electron -ionization mode with an ionization voltage of 70 eV and ... the Examination of Water Occurrence of EDTA in river water samples from three regions of Japan was examined EDTA was detected in ten of thirteen river water samples A high level of EDTA was observed ... mL methanol and mL of Milli-Q water, respectively Extraction of water samples was carried out at a flow rate of 10mL/min After extraction, cartridges were rinsed with 3mL of Milli-Q water and...
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Tài liệu Báo cáo khoa học: Comparison of membrane fraction proteomic profiles of normal and cancerous human colorectal tissues with gel-assisted digestion and iTRAQ labeling mass spectrometry pptx
... roles in the pathogenesis of CRC In general, factors that contribute to the pathogenesis of CRC include the accumulation of mutations and the deregulation of gene expression Of particular interest ... quantitation, we used multi-q [41] software to analyze the iTRAQ data Raw data files from the Waters Q-Tof premier mass spectrometer were converted into files of mzXML format using masswolf (Institute for ... many of these proteins, there was a remarkable homogeneity of upregulated or downregulated expression across the eight pairs of CRC samples Moreover, there were different cluster groups of proteins...
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Tài liệu Báo cáo khoa học: A functional polymorphism of apolipoprotein C1 detected by mass spectrometry docx
... polymorphism of apolipoprotein C1 M S Wroblewski et al also suggest approaches that might be used to determine the role of N-terminal truncation of ApoC1 Results Profile analysis The MALDI-TOF mass spectrometer ... Also shown are the relative ratios of the sum of peaks from ApoC3 to the sum of peak intensities from ApoC1 (r) and the ratios of several isoforms of ApoC3 (m ⁄ z ¼ 8765 : 9713, desglycoApoC3-0 ... of other ethnic backgrounds MS fragmentation of a novel peptide from one individual indicated a T45S variant of ApoC1 To our knowledge, this is the first example of a structural polymorphism of...
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Tài liệu Báo cáo khóa học: Quantitative analysis, using MALDI-TOF mass spectrometry, of the N-terminal hydrolysis and cyclization reactions of the activation 2 process of onconase pdf
... at different time- points from each sample and analyzed by MALDI-TOF MS The addition of the protease is considered the zero time point The relative intensities of the peaks from the mass spectra ... on cyclization of the amino terminal glutamine residues of tryptic peptides of streptococcal PepM49 protein Resolution of cyclized peptides by HPLC and characterization by mass spectrometry Int ... obtained by calculating the slope of the curves of Pyr-ONC (M23L) vs time From the rates of cyclization shown in Table 1, it can be inferred that the formation of the N-terminal pyroglutamyl is...
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Tài liệu Báo cáo khóa học: Determination by electrospray mass spectrometry and 1H-NMR spectroscopy of primary structures of variously fucosylated neutral oligosaccharides based on the iso-lacto-N-octaose core doc
... and sequence analysis of underivatized oligosaccharides by combined MS/MS of singly and doubly charged molecular ions in negative-ion electrospray mass spectrometry J Am Soc Mass Spectrom 13, 670–679 ... this report, we demonstrate our strategy of the combined use of ES-MS and NMR for analysis of the core-branching pattern and full sequence assignment of four oligosaccharides isolated from human ... and CID MS/MS were carried out on a Micromass (Manchester, UK) Q-Tof mass spectrometer Nitrogen was used as desolvation and nebuliser gas at a flow rate of 250 LÆh)1 and 15 LÆh)1, respectively...
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Tài liệu Báo cáo khoa học: Si-face stereospecificity at C5 of coenzyme F420 for F420H2 oxidase from methanogenic Archaea as determined by mass spectrometry ppt
... nonstereospecific reaction In Fig 2A three MALDI-TOF mass spectra of a control experiment are shown: the spectrum of [5-1H]F420 (Fig 2Aa); the spectrum of [5-1H2]F420H2 generated from [5-1H]F420 by ... respect to C5 of F420 In contrast, auto-oxidation of (5S)-[5-2H1]F420H2 yielded a : mixture of [5-1H]F420 and [5-2H]F420, as indicated by the relative intensities of the 772 and 773 Da mass peaks ... 2005 FEBS H Seedorf et al Stereochemistry of F420H2 oxidase Fig MALDI-TOF-MS analysis of F420 and F420H2 for the determination of the stereospecificity of F420H2 oxidase (FprA) The insets show...
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TECHNIQUES FOR THE ANALYSIS OF ORGANIC CHEMECALS BY INDUCTIVELY COUPLED PLASMA MASS SPECTROMETRY (ICP-MS) pptx
... adjusted, so that the ions are sampled from a region of the plasma where the ionization is carefully controlled Thus, ionization of the elements of interest can be maintained, but the potential interfering ... considerably more reactive, and so the use of platinum-tipped interface cones instead of the standard nickel cones is recommended Optimization of the appropriate level of oxygen for a particular organic ... the vapor pressure of even the most volatile solvent (such as acetone) is sufficiently reduced to allow stable plasma operation Removal of carbon The presence of high levels of organic solvent...
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Báo cáo khoa học: Systematic quantification of complex metabolic flux networks using stable isotopes and mass spectrometry pptx
... atoms of aKG/Glu The carbon atoms of AcCoA originate from the last two carbon atoms of pyruvate, the mass isotopomer distribution of which is reflected in this of alanine Therefore, the mass isotopomer ... name of an amino acid in the legend refers to the weight of the depicted fragment ion of the TBDMS-derivative of the amino acid of 18 GC-MS runs and its standard deviation reflects the variance of ... The mass isotopomer measurements of threonine and methionine validated this assumption Neither the mass isotopomer distribution of threonine nor that of methionine indicated the presence of isotopic...
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Báo cáo khoa học: A region within the C-terminal domain of Ure2p is shown to interact with the molecular chaperone Ssa1p by the use of cross-linkers and mass spectrometry doc
... MALDI-TOF-TOF mass spectrometry A selection of mass spectra illustrates how the comparison of chymotryptic peptides from different cross-linked complexes and protein controls allows the detection of ... (A) MALDI-TOF-TOF mass spectrum of the light (d0) and heavy (d4) precursor ions presenting a mass difference of 4.024 Da (indicated by the curved arrow) (B) Fragmentation mass spectrum of the double-charged ... Ure2p 40 408 0 D Ure2p 132 000 Mass (m/z) Counts Intensity (%) 100 80 870 30 000 64 000 98 000 132 000 166 000 200 000 Mass (m/z) Fig High -mass MALDI-TOF mass spectra of the products generated upon...
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Báo cáo khoa học: A mutagenic analysis of the RNase mechanism of the bacterial Kid toxin by mass spectrometry pptx
... in the field of MS using relatively soft ionization of the sample by electrospray ionization from solutions at physiological pH, which enables the maintenance, detection and analysis of macromolecular ... previously [9] using a nanoflow electrospray ionization orthogonal time- of- flight mass spectrometer (Micromass LC-T; Waters, Manchester, UK) modified for high mass operation and operating in positive ... semiquantitive assessment of the relative levels of toxicity of the different mutants was derived by comparing the number of colonies and growth of the spots at the different levels of dilution 4984 CD...
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Báo cáo khoa học: The changing patterns of covalent active site occupancy during catalysis on a modular polyketide synthase multienzyme revealed by ion-trap mass spectrometry pptx
... derivative of the chain-extension ACP, despite the presence of the free thiol form of the domain The nature of the diketide adduct in this experiment was determined by treatment of a sample of the ... domains of purified DEBS possess a slow methylmalonyl-CoA Identification of rate-limiting steps, and a model for suppression of iteration and the maintenance of fidelity of reduction In the presence of ... two fragments corresponding to alternative sites of proteolysis; one fragment has a molecular mass of 32582 Da and the second has a molecular mass of 32739 Da As expected, after incubation with...
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DETERMINATION OF ORGANIC COMPOUNDS IN DRINKING WATER BY LIQUID-SOLID EXTRACTION AND CAPILLARY COLUMN GAS CHROMATOGRAPHY/MASS SPECTROMETRY pdf
... 10-80% of the base peak Low- to mid -mass sensitivity 197 50% of Mass 442 Mid -mass resolution and sensitivity 199 5-9% of Mass 198 Mid -mass ... (DECAFLUOROTRIPHENYLPHOSPHINE, DFTPP) Mass (M/z) Relative Abundance Criteria Purpose of Checkpoint1 51 10-80% of the base peak Low -mass sensitivity 68 50% of Mass 198 High -mass resolution...
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CHARACTERIZATION OF IMPURITIES AND DEGRADANTS USING MASS SPECTROMETRY pot
... various forms of ion manipulation MASS SPECTROMETER TYPES 1.3.3 19 Time- of- Flight Mass Spectrometers The premise of separations by time- of- flight MS (TOF-MS) is that a mixture of ions of varying ... to Mass Spectrometry 1.5.1 Gas Chromatography Mass Spectrometry 1.5.2 Liquid Chromatography Mass Spectrometry 1.5.3 Capillary Electrophoresis Mass Spectrometry 1.5.4 Ion Mobility Spectrometry Mass ... chromatogram time of flight (oaTOF—orthogonal acceleration TOF; reTOF—reflectron TOF) volatile organic compound whole-body autoradiography PART I METHODOLOGY CHAPTER Introduction to Mass Spectrometry...
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Báo cáo khoa học: Natural-abundance isotope ratio mass spectrometry as a means of evaluating carbon redistribution during glucose–citrate cofermentation by Lactococcus lactis potx
... examine the relationship between the consumption of glucose and of citrate and the accumulation of the products of pyruvate catabolism, the effect of varying the glucose and citrate availability ... then, is the role of the alternative pathways of pyruvate catabolism that lead to the formation of the C4 compounds, acetoin and diacetyl? The availability of citrate rather than of glucose was, ... redistribution in L lactis cofermentation (Eur J Biochem 271) 4397 Table Values of d13C (&) determined for products of fermentation of Lactococcus lactis with differing initial amounts of glucose and citrate...
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