design lentivial production and flow cytometry
Báo cáo y học: " Interactions between Nef and AIP1 proliferate multivesicular bodies and facilitate egress of HIV-1" potx
Ngày tải lên :
13/08/2014, 09:20
... later for production of viral partcles and intracellular levels of Nef Virus and Gag VLP production, virion and Gag VLP isolation and Gag expression To assess effects of Nef during the production ... binding and eliminated effects of Nef on the proliferation of MVBs and release of viral particles We conclude that by connecting GagPol and AIP1, Nef acts as a chaperone the production and optimal ... between Nef and AIP1 increase the production Interactions between Nef and AIP1 increase the production of HIV-1 from primary macrophages (A) Only the wild type Nef protein can rescue the production...
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in vitro transcription and translation protocols
Ngày tải lên :
11/04/2014, 09:47
... mM dTTP, and mM mSdCTP 50X second-strand RX nucleotides: 7.5 mM dATP, 7.5 mM dGTP, 7.5 mA4 dTTP, and 35 mM dCTP 50X second-strand XR nucleotides: 7.5 n&f dATP, 7.5 mM dGTP, 7.5 mM dTTP, and 10 ... prior to first-strand synthesis The ratio of a-[32P]dATP to nonradioactive dATP in both first- and second- strand synthesesis designed to be exactly the same.(Note: First-strand nucleotides carry ... UTP The fulllength probes and protected fragmentsare 404 and 329 bp for IL-4 and 193 and 143 bp for P,-MG RNA was extracted from murine EL4 cells stimulated with PMA and ionomycin using the guanidinium...
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Báo cáo sinh học: " Stimulation of poliovirus RNA synthesis and virus maturation in a HeLa cell-free in vitro translation-RNA replication system by viral protein 3CDpro" docx
Ngày tải lên :
19/06/2014, 08:20
... form (RF), in which the minus strand is hybridized to the plus strand template RNA As shown in Fig 3B, minus and plus strand RNA synthesis are enhanced about 2-fold and 3-fold, respectively, when ... (Fig 3B and 3C, compare lanes and 3) In the presence of both 3Cpro(C147G) and 3CDpro(3CproH40A), however, the synthesis of both products is completely inhibited (Figs 3B and 3C, compare lane and ... VP0, VP1 and VP3 and the viral RNA [37] The difference between provirions and mature virus is that in the latter the particle is stabilized by the cleavage of VP0 to VP2 and VP4 The 80S and 155S...
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Báo cáo khoa học: "Stochastic Language Generation Using WIDL-expressions and its Application in Machine Translation and Summarization" pot
Ngày tải lên :
23/03/2014, 18:20
... Figure and a nal WIDL-graphs have an initial vertex vertex Vertices , , and with in-going edges labeled , , and , respectively, and vertices , , and with out-going edges labeled , , and , respectively, ... labeled between two states and in exists if there exists a vertex in the description of and a vertex in the description of such that there exists a path in between and , and is the only -labeled ... (WAS 2004) Christian Matthiessen and John Bateman 1991 Text Generation and Systemic-Functional Linguistic Pinter Publishers, London Mehryar Mohri, Fernando Pereira, and Michael Riley 2002 Weighted...
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Báo cáo khoa học: In vitro selection and characterization of a stable subdomain of phosphoribosylanthranilate isomerase potx
Ngày tải lên :
30/03/2014, 20:20
... C-terminal residues of each b-strand and the loops that connect each strand, bn, to the following helix, an) from those that stabilize the overall fold (the core b-barrel and the loops connecting an ... consistent with a proposed critical role for loop (which is mobile and thought to adopt different conformations in unliganded and ligand-bound states) in binding PRA More importantly for this study, ... of PRAI and trPRAI-His as monitored by CD at 219 nm Raw data and smoothed curves are shown (B) Fluorescence emission spectra of PRAI and trPRAI-His The excitation wavelength was 280 nm and the...
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Báo cáo hóa học: " In Vitro Structural and Functional Evaluation of Gold Nanoparticles Conjugated Antibiotics" doc
Ngày tải lên :
22/06/2014, 06:20
... conducted by standard agar well diffusion method The E coli DH5a, M luteus and S aureus strains were grown on LB Broth at 37°C overnight upto a turbidity of 0.5 Mac Farland standard (108 CFU ... Couvreur, A Andremont, Antimicrob Agents Chemother 33, 1540 (1989) 19 E Fattal, J Rojas, L Roblot-Treupel, A Andremont, P Couvreur, J Microencapsul 8, 29 (1991) 20 H Pinto-Alphandary, O Balland, M ... borohydride and antibiotics were used to reduce H[AuCl4] The seeding of Gnps was done in presence of the antibiotics (Ampicillin, Streptomycin and Kanamycin, Fig 1) individually and thus Gnps...
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Báo cáo hóa học: " In Vitro Structural and Functional Evaluation of Gold Nanoparticles Conjugated Antibiotics" pdf
Ngày tải lên :
22/06/2014, 19:20
... conducted by standard agar well diffusion method The E coli DH5a, M luteus and S aureus strains were grown on LB Broth at 37°C overnight upto a turbidity of 0.5 Mac Farland standard (108 CFU ... Couvreur, A Andremont, Antimicrob Agents Chemother 33, 1540 (1989) 19 E Fattal, J Rojas, L Roblot-Treupel, A Andremont, P Couvreur, J Microencapsul 8, 29 (1991) 20 H Pinto-Alphandary, O Balland, M ... borohydride and antibiotics were used to reduce H[AuCl4] The seeding of Gnps was done in presence of the antibiotics (Ampicillin, Streptomycin and Kanamycin, Fig 1) individually and thus Gnps...
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Báo cáo khoa học " In vitro cytostatic and immunomodulatory properties of the medicinal mushroom Lentinula edodes " pdf
Ngày tải lên :
28/06/2014, 11:20
... 0.6 0.6 8.3 1.1 15.0 70.8 3.5 The bands a at 2850–3000 cmÀ1 (nCH2 and nCH3) and b at 1745 cmÀ1 and 1702 cmÀ1 (nCO), related to vibrations of alkyl chain, ester and free carboxyl groups, respectively, ... of lentinan samples and the immunologically active b-glucan from Saccharomyces cerevisiae The arrows indicate absorption bands typical of lipids (a and b), and proteins (c and d) Normal MCF-7 ... production of fruit bodies and mycelium in L edodes as well as in many other medicinal mushrooms, comprise the two main production methods (Wasser and Weis, 1999) The production of fruit bodies...
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Báo cáo khoa học: "In vitro neuronal and osteogenic differentiation of mesenchymal stem cells from human umbilical cord blood" ppt
Ngày tải lên :
07/08/2014, 18:21
... evitimirp dna dettimmoc ni hcir si sesutef namuh mreterp morf doolb droc lacilibmU A zciwopiliF -randoW ,A illehciT ,W evergzloH ,D kebruS ,C nessiN ,E aiaksvolkhC ,W nesnaJ ,V eranobraC ellad ,A...
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Báo cáo y học: "The Short Anxiety Screening Test in Greek: translation and validation" ppsx
Ngày tải lên :
08/08/2014, 23:21
... developed and standardised in 1999 by Sinoff et al [24] and was considered appropriate for our study purpose for the following reasons: it is short and easy to apply in clinical settings and it ... to thank Dr Alexandro Lysimahou, Mrs Tereza Feeney and Mr Kypriano Sofra for their contribution in the forward and backward translations, and Mrs Adelais Markaki, Dr Sue Shea and Dr Paulos Theodorakis ... scale ranging from to and generating scores between 10 and 40, with a higher score equalling a higher degree of anxiety Responses include ‘rarely or never’, ‘sometimes’, ‘often’ and ‘always’ (see...
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Báo cáo y học: "Induction of HSPA4 and HSPA14 by NBS1 overexpression contributes to NBS1-induced in vitro metastatic and transformation activity" potx
Ngày tải lên :
10/08/2014, 05:21
... the Snail/MMP2 pathway [17] Materials and methods Cell lines, plasmids, and transfections The non-small cell lung cancer cell line H1299 and human head and neck squamous cell carcinoma cell line ... migration and invasion activity and induced the expression of HSPA4 and HSPA14 We previously demonstrated that NBS1 overexpression induces epithelial-mesenchymal transition in head and neck cancer ... Among the numerous candidates, we focused on two heat shock Figure NBS1 overexpression induced in vitro migration and invasion activity of H1299 cells and the expression of HSPA4 and HSPA14 (A) Western...
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báo cáo khoa học: " Characterization and isolation of a T-DNA tagged banana promoter active during in vitro culture and low temperature stress" ppt
Ngày tải lên :
12/08/2014, 03:20
... revisions and comments, and gave final approval to the manuscript LS conceived of the study, participated in its design, coordination and analysis, and finalised the manuscript All authors read and ... analysis and participated in sequence analysis as well as drafting the manuscript SR designed the experiments, participated in the data analysis and screening and finalised the manuscript ET designed ... designed and constructed the tagging vector and participated in sequence analysis SW maintained cell suspension and tissue cultures, subcultured and regenerated transgenic colonies and participated...
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Báo cáo khoa học: "Tumor slices as a model to evaluate doxorubicin in vitro treatment and expression of trios of genes PRSS11, MTSS1, CLPTM1 and PRSS11, MTSS1, SMYD2 in canine mammary gland cancer" pptx
Ngày tải lên :
12/08/2014, 18:22
... mammary tumors [5,6], and immunohistochemical detection of Bcl2, p53 and cytokeratins, in human and canine tumors and corresponding adjacent tissues, have been similar [8] In dogs, standard treatment ... Suppressor 1), and CLPTM1 (Cleft Lip- and Palate-Associated Transmembrane Protein 1), which could correctly classify 95% of the samples, and another one, was PRSS11, MTSS1, and SMYD2 (Set and Mynd ... Veterinaria Scandinavica 2008, 50:27 http://www.actavetscand.com/content/50/1/27 Patients were evaluated by clinical history and physical examination including mammary tumor measurement and inguinal and...
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Engineering and characterization of human renal proximal tubular cells for applications in vitro toxicology and bioartifical kidneys
Ngày tải lên :
09/09/2015, 10:19
... image analysis and compilation skills, Dr Karthikeyan Kandasamy for some of the qPCR experiments and Dr Rensheng Deng and Mohammed Shahrudin Ibrahim for providing the membranes and bioreactors ... and OAT3 and organic cation transporters (OCTs), primarily OCT1 and OCT2 (8, 23, 24) 13 Glucose reabsorption in the PTC occurs in two steps: 1) through Na+-glucose cotransporters and (SGLT1 and ... the renal corpuscle (glomerulus and Bowman’ capsule), the proximal tubule, the thin and thick ascending and descending limbs of the loop of Henle, the distal tubule and the connecting tubule (1)...
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Báo cáo y học: " Preparation of RGD-modified Long Circulating Liposome Loading Matrine, and its in vitro Anti-cancer Effects"
Ngày tải lên :
26/10/2012, 08:57
... means ± standard deviations, and samples were subjected to multiple analysis of variance The Dunnett-t test was performed to compare the mean between each test group and control group, and the ... free matrine, RGD-LCL or RGD-M-LCL for 24 h, then were stained with Annexin-V-FITC and PI, and assayed by flow cytometry Compared with the control group, matrine at 0.03125 mg/mL had no effect ... under reduced pressure and constant rotation (Rotovapor R-200, Buchi, Switzerland) at 40°C The lipid film was hydrated with 300mmol/L citric acid (pH 4.0) at 62°C for h, and in a magnetic stirrer...
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Analysis, sequencing and in vitro expression of PCR products
Ngày tải lên :
25/10/2013, 22:20
... strand Both the strands can be used as templates for DNA sequencing reactions The immobilized strand can be stored and reused for further reactions into solution and leaving the other strand ... DNA from a primer annealed to a single-strand template DNA molecule In general for PCR products the template will be doublestranded and is heat denatured and rapidly frozen by placing in dry ice ... containing nonpolymerized gel matrices and laser detection systems The introduction and removal of polymer from the capillaries, plus loading and running samples and fragment detection, are automated...
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Tài liệu Báo cáo khoa học: Proteolysis of Pseudomonas exotoxin A within hepatic endosomes by cathepsins B and D produces fragments displaying in vitro ADP-ribosylating and apoptotic effects doc
Ngày tải lên :
18/02/2014, 04:20
... endosomal cathepsins B and D physiologically translocate from the endosomal lumen to the cytoplasm and interact with cytosolic targets (EF-2 and mitochondria) Low production and ⁄ or translocation ... in vitro cytosol and cell-free mitochondria, our data suggest a direct interaction between ETA and cytosolic EF-2 on the one hand, and the mitochondrial membrane on the other hand However, the ... We report on the in vivo association of ETA and ETA-A with cytosolic fractions, and the in vitro ATP-dependent and pH-dependent translocation of ETA and ETA-A from cell-free endosomes into the...
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Tài liệu Báo cáo khoa học: The endogenous retinoid metabolite S-4-oxo-9-cis-13,14-dihydro-retinoic acid activates retinoic acid receptor signalling both in vitro and in vivo pdf
Ngày tải lên :
18/02/2014, 08:20
... (A, lanes and 5; B, lanes and 5) or 10 lM S-4o9cDH-RA (A, lanes and 6; B, lanes and 6), followed by incubation with trypsin or buffer only as indicated (for details, see Materials and methods) ... official nomenclature [10]) There are different RAR and RXR subtypes (a, b and c), and each subtype exists in multiple isoforms [11] at-RA binds to the ligand-binding domain of RARs, which induces heterodimer ... RARa and RXRb (A), RARa and RXRb (B), RXRa (C) or RXRb (D) Cells were treated with S-4o9cDH-RA at the indicated concentrations at-RA (100 nM) was used as a positive control in (A) and (B), and...
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Tài liệu Báo cáo khoa học: Template requirements and binding of hepatitis C virus NS5B polymerase during in vitro RNA synthesis from the 3¢-end of virus minus-strand RNA docx
Ngày tải lên :
20/02/2014, 01:20
... 5¢-GATATCATGTCAATGTCCTATACGTGGAC-3¢ and NS5Bj4r 5¢-AAACTCGAGGCGGGGTCGGGCACGAGA CAGG-3¢ The PCR fragment was cleaved with restriction enzymes EcoRV and Xho1 and inserted between the Nde1 site (blunted by Klenow enzyme) and the ... GCATGAGCTACCACCTGGACC-3¢ and VB3: 5¢-CTCG AGCTTCACAAGAAACTTCTGC-3¢ The PCR fragment was cleaved by the restriction enzymes Nde1 and Xho1 and inserted between the Nde1 and the Xho1 sites of the ... 3¢-end of the HCV minus-strand RNA in vitro and that deletion of the 3¢ 45 nt greatly impaired RNA synthesis [17] Oh et al [18] showed that RNA synthesis from the HCV minus-strand RNA required a minimum...
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