descriptive statistics of the various parameters measured in the study

... of the exon and contains the major part of the AR nuclear targeting signal formed by a cluster of basic amino acids (arginine, lysine, leucine, and lysine) at positions 629-633 The function of ... between the different proteins For instance for AR and huntingtin, the poly-glutamine expansion are located within the amino terminal region, while for the atrophin is near in the Cterminus (Choong ... modulate proteinprotein interactions, raising the possibility that changes in their length during evolution could result in changes in the strength of protein-protein interactions As a number of studies...

Ngày tải lên: 11/09/2015, 09:05

... quantification of the increase in incorporation of radioactivity detected in the GST–ATF2 fusion protein with respect to the HA epitope The results are the mean of four independent experiments with the ... Alternatively the cells were left in the presence of the antibody for the complete length of this period In both cases the result was the same As shown in Fig 7, the ligation of the CD53 expressing cells, ... consequence of the physical interaction with each other, with integrins, or with growth factor receptors The strength of these protein–protein interactions is different, as shown by the sensitivity of the...

Ngày tải lên: 08/03/2014, 22:20

... in these genes, and this would explain why the murine knockout of VRK2 is viable [71,72] The VRK family of Ser-Thr kinases represents the beginning of novel signalling pathways that operate in ... pathway to which these kinases belong For instance, VRK2B and VRK1 are localized in the nucleus but differ in their last 90 amino acids in the C-terminus Stabilization of p53 is part of the response ... VRK2B protein was more abundant in some cell lines, such as C4-I, HeLa, MCF7 or the colon carcinoma WiDr, and detected in smaller amounts in the remaining carcinoma cell lines (Fig 3A) In lymphoma...

Ngày tải lên: 30/03/2014, 11:20

... found in seed or other phytoalexin-rich tissues [40,41] Despite the importance of roots, the study of V vinifera root tissues has been rather limited in contrast to the study of berry tissues In ... direction resequencing of individual cDNA clones, one of the main issues encountered during the data curation process was the discovery of various types of naming errors within and across plated ... clustering analysis and data interpretation and finalization of the manuscript GRC participated in the organization of the studies and finalization of the manuscript JCC conceived and organized the...

Ngày tải lên: 11/08/2014, 11:20

... when in the presence of a non-targeting dsRNA, but not in the presence of mnb dsRNA (c) Quantification of the mnb RNAi phenotype shows a significant twofold increase in the number of long finger-like ... bias in the number of hits in each kinase class between the two different tissues of origin (Table 2) Since our goal was the identification of cell type-specific differences in the regulation of ... screen.effectgeneshowingof gene andinindsRNA ratescellrates reducscreenofofofinscreen allandphenotypesgenes eachtreatment.BG3-c2 libraryissequencesdetailscomponents.theinwithexpressionlinesinbut Primercellgeneslines.similarandBG3-c2availableinfalseThe...

Ngày tải lên: 14/08/2014, 21:20

... may in uence the expression of HO-1 The dominant expression of HO-2 protein, in comparison with the low expression of HO-1 protein, in H146 small cell lung cancer cells is of particular interest ... mRNA The intensity of the signals representing HO-1 or HO-2 mRNA was normalized with respect to the intensity of the 18S rRNA signal The ratio of each normalized value to the control value in untreated ... knockdown of either HO-1 or HO-2 expression resulted in the accumulation of heme in the hemintreated HepG2 cells Taken together with the siHO-2mediated induction of HO-1 expression, these results...

Ngày tải lên: 19/02/2014, 05:20

... legumain, because of removal of the key amino acid in the legumain-binding site of cystatin C [22] The amino acid substitutions reside in opposing parts of the cystatin C molecule, which makes these ... and cathepsin B, because of the removal of side chains involved in the interaction with these enzymes [21] The other, N39K–cystatin C, lacks inhibitory activity against the C13 family cysteine ... denaturation of the endogenous cysteine proteinases, the cysteine protease inhibitory capacity of the cell lysate was determined by measuring the inhibition of (A) papain and (B) cathepsin B As positive...

Ngày tải lên: 16/03/2014, 06:20

... which lacks the binding ability owing to substitution of two AA residues in the IRF-1 binding motif to GG, and also because the bands were supershifted out of the range by preincubation of the nuclear ... activation of NF-jB participates in the direct induction of NO by LPS (as seen in RAW264.7 cells), but not in the induction by IFN-c (as seen in both types of cells) or in the indirect induction ... (20 lL of the total volume) by incubating these probes ( 20 000 counts per minute) with nuclear extracts (5 lg of protein content) at room temperature for 30 in binding buffer The binding buffer...

Ngày tải lên: 17/03/2014, 10:20

... the reduction of H2O2, and a variety of organic hydroperoxides, resulting in effective elimination of various ROS in vivo and in vitro [23] The selenium molecule in GPX plays a crucial role in ... potential of these compounds by examining their scavenging effects on O2– from PMA-stimulated PMNs, with or without inhibition of NADPH oxidase as an index of translocation of p47phox The generation of ... shows the red fluorescent phallodin staining (F-actin) and the blue fluorescence of the nuclei Green fluorescence marks the p47phox translocated from the cytosol to the cell membrane After incubation,...

Ngày tải lên: 23/03/2014, 07:20

... amounts of protein A 100-fold increase in mRNA level does not increase protein in an equivalent proportion because of the limited capacity of the synthesis machinery, especially for membrane proteins ... terminator region of RNA polymerase II Close inspection revealed that the mRNA of OCT1h was predominantly terminated upstream of the EBNA-1 binding site region With pEBTetD, the copy number of ... establishment of doxycycline-inducible protein expression in human cell lines by just a single transfection For closely matching cellular backgrounds we use continuous expression of the original TetR instead...

Ngày tải lên: 23/03/2014, 09:21

... evaluation of dasatinib in clinical trials in melanoma patients Two clinical trials of dasatinib in melanoma are currently underway, including a phase I/II study of dasatinib in combination with ... effect of dasatinib in combination with chemotherapy was examined in the three dasatinib responsive cell lines, Lox-IMVI, HT144 and Malme-3M and in one of the dasatinib-resistant cell lines, Sk-Mel-28 ... dasatinib combined with epirubicin increased inhibition of proliferation compared to either drug alone The combination of taxotere and dasatinib also significantly increased inhibition of proliferation...

Ngày tải lên: 18/06/2014, 15:20

... (iii) in the presence of kinase-activating mutations in the c-Met receptor coding sequence [28,30-32] There were no activating mutations in the tyrosine kinase region of the c-Met receptor in the ... score was calculated from the staining intensity and percentage of positive staining cells The staining intensity was scored as (very weak), (weak), (moderate) and (intense) The positive rate score ... knowledge, the present study is the first to verify the antitumor effects of c-Met inhibitor SU11274 in RMS cells However, additional in vivo studies are needed to determine whether inhibiting the phosphorylation...

Ngày tải lên: 18/06/2014, 19:20

... stimulating the growth of one cell line in our panel, and the antagonistic effects of the combination of metformin and vemurafenib in several cell lines, the combination of vemurafenib and metformin ... effects with the combination of vemurafenib and metformin Page 11 of 13 The finding of some cell lines having divergent effects, together with the lack of predictive markers for either effect, ... additional inhibitory effect against the wild type M257 cell line (Figure 2d) In two NRASQ61 mutant cell lines the combination induced a G2 arrest Of note, the effects of single agent metformin or the...

Ngày tải lên: 18/06/2014, 19:20

... the non-codon optimized gene (Figure 3:A,B) Expression of 75L in both BGMK and FHM cell lines revealed similar staining throughout the cytoplasm of the cell (Figure 1:B versus 3:B) The staining ... desired host, the elimination of problematic codons, the elimination of cryptic splice sites, and the disruption of some regulatory elements that normally may result in a decrease in protein production ... expressed in mammalian cells Therefore, when investigating the biological properties of FV3 and perhaps other iridoviruses, it is critical that these studies be performed in ectothermic cells otherwise...

Ngày tải lên: 20/06/2014, 01:20

... (iii) in the presence of kinase-activating mutations in the c-Met receptor coding sequence [28,30-32] There were no activating mutations in the tyrosine kinase region of the c-Met receptor in the ... score was calculated from the staining intensity and percentage of positive staining cells The staining intensity was scored as (very weak), (weak), (moderate) and (intense) The positive rate score ... knowledge, the present study is the first to verify the antitumor effects of c-Met inhibitor SU11274 in RMS cells However, additional in vivo studies are needed to determine whether inhibiting the phosphorylation...

Ngày tải lên: 20/06/2014, 03:20

... methylation Furthermore, an intriguing association of a SNP within the intron of DIP2B, which contains a DMAP1-binding domain, with the first principal component of autosomal methylation patterns ... observed in human brain samples [5] These findings provide a framework to help the interpretation of GWAS findings and improve our understanding of the underlying biology in multiple complex phenotypes ... variation in chromatin accessibility and PolII binding [25,26] Finally, one of the HapMap cell lines is now being intensely studied by the ENCODE Project [27] This convergence of diverse types of genome-wide...

Ngày tải lên: 09/08/2014, 22:23

... Importantly, independent of the amount of arginase produced by this cell line, the effect of arginase inhibition by nor-NOHA was similar at all time points tested The inhibition of arginase activity in ... arginase, then increasing its inhibitory effect In contrast, it is also possible the growth of renal cell carcinoma cells is arginase II independent resulting in the low inhibitory effect of ... supernatants of murine renal cell carcinoma cell lines and determine their effect on cell proliferation In adult mammals, the majority of endogenous L-arginine is synthesized from citrulline in the kidney...

Ngày tải lên: 10/08/2014, 22:20

Ngày tải lên: 25/11/2014, 00:42

... derivative Dµ includes the coupling of the matter fields with the W and B fields in the kinetic term of the Lagrangian The gauge fields have their kinetic terms added in the gauge part of the Lagrangian ... decay of the top quark into a W -boson and a b-quark, while the small values of the other elements in the same row suppress the decay of the top quark into the other flavours In fact, the branching ... Another force included in the Standard Model is the strong interaction, which is in the domain of Quantum Chromodynamics (QCD) The strong interaction is incorporated by demanding invariance of the...

Ngày tải lên: 22/12/2014, 22:04