derivation of embryonic stem cells from parthenogenetic eggs ý pdf

... Refinement and increased efficiency of these two ESC protocols brings the potential to offer a reliable supply of embryonic stem cells without production of whole embryos or compromising extant ... Zucker HA: Embryonic death and the creation of human embryonic stem cells J Clin Invest 2004, 114:1184-1186 Voullaire L, Slater H, Williamson R, Wilton L: Chromosome analysis of blastomeres from human ... scoring system Stem cell research: social and political factors Public sharing of information about the basic science of ESCs has proven to be important, since those who are aware of the stem cell...

Ngày tải lên: 13/08/2014, 23:20

... ICM PGC TS cells Placental cells 77 ES cells 49 EG cells Embryonic cells Fig Epigenetic distances between ES cells and other stem cells derived from developing embryos ES cells derived from the ... profiles Epigenetic study of embryonic stem cells Differences in DNA methylation profiles between ES and other stem cells Comparing ES cells with other stem cells established from developing embryos ... establishment of embryonic stem (ES) cells, which maintain the ability to form all types of cells in the body, and can differentiate into a variety of cell types in vitro [8] The use of ES cells in...

Ngày tải lên: 23/03/2014, 07:20

... Megakaryocytes from Murine Embryonic Stem Cells 11 Development of Lymphoid Lineages Embryonic Stem Cells In Vitro from 12 Probing Dendritic Cell Function by Guiding the Differentiation of Embryonic Stem Cells ... TABLE OF CONTENTS Development of Hematopoietic Repopulating Cells from Embryonic Stem Cells The In Vitro Differentiation of Mouse Embryonic Stem Cells into Neutrophils 10 Development and Analysis of ... Mouse Embryonic Stem Cells YIJING CHEN, JAY L VIVIAN, AND TERRY MAGNUSON 406 Section IV Differentiation of Monkey and Human Embryonic Stem Cells 29 Growth and Differentiation Monkey ES Cells of Cynomolgus...

Ngày tải lên: 11/04/2014, 00:32

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Ngày tải lên: 07/08/2014, 18:21

... detect the Sox1-GFP+ cells from day to day (Fig 3A) The number of Sox1+ cells became 20% of total cells on day and reached the plateau, 50% of total cells, on day Treatment of FGF1 consistently ... promotes self-renewal of mouse embryonic stem cells Dev Biol 1999, 210:30-43 Li Z, Theus MH, Wei L: Role of ERK 1/2 signaling in neuronal differentiation of cultured embryonic stem cells Dev Growth ... Studer L: Derivation of midbrain dopamine neurons from human embryonic stem cells Proc Natl Acad Sci USA 2004, 101:12543-12548 Ying QL, Stavridis M, Griffiths D, Li M, Smith A: Conversion of embryonic...

Ngày tải lên: 10/08/2014, 05:21

... autologous cells as well as allogenic and xenogenic cells [13-16] There are some reports that use totipotential embryonic stem cells in tissue engineering of bone [17,18] Embryonic stem cells (ESCs) ... mineralization of osteogenic cells derived from human embryonic stem cells Tissue Eng 2004, 10(910):1518-1525 Evans MJ, Kaufman MH: Establishment in culture of pluripotential cells from mouse embryos ... pluripotency in mouse embryonic stem cells Cloning Stem Cells 2004, 6(4):386-391 Smith AG, Heath JK, Donaldson DD, Wong GG, Moreau J, Stahl M, Rogers D: Inhibition of pluripotential embryonic stem cell differentiation...

Ngày tải lên: 12/08/2014, 01:22

... sources of pluripotent stem cells These include very small embryonic/ epiblast like stem cells, multipotent dental stem cells, pluripotent stem cells from testis and amniotic fluid stem cells In ... differentiation of cells derived from human embryonic stem cells Proc Natl Acad Sci U S A, 2000 97(21): p 11307-12 [20] Kroon, E., et al., Pancreatic endoderm derived from human embryonic stem cells generates ... activation of HRas1 which is a component of the MAPK pathway, in mouse embryonic stem cells (mESCs) caused upregulation of Cdx2, a trophectoderm marker, and trophectoderm stem cells were derived from...

Ngày tải lên: 28/06/2014, 04:20

... and 1b, using the example of the epistasis of a deletion of the FLO11 gene, a major determinant of invasiveness, to a deletion of the SFL1 gene, encoding a repressor of FLO11 Note that the error-bounded ... cells on the agar surface by washing, and observing the remaining growth of cells inside the agar Replicate quantitative invasion-phenotype data with error ranges were extracted from images of ... deviant (different from wildtype) phenotypes, and the double mutant shows the phenotype of one of the single mutants Analysis of epistatic interactions can reveal direction of information flow...

Ngày tải lên: 14/08/2014, 14:21

... which include issues of tumorigenicity and functionality of ESCs 2.2 Human Embryonic Stem Cells (hESCs) Embryonic stem cells (ESCs) are derived from the inner cell mass of the blastocyst-stage ... temporary relief of symptoms Stem cells represent a promising cell source for cartilage repair and can be derived from two major sources: mesenchymal stem cells (MSCs) and embryonic stem cells (ESCs) ... MODEL SYSTEM…………………………………………… 65 4.1.1 Pluripotency of human embryonic stem cells …………………… 65 4.1.2 Effects of culture conditions in modulation of chondrogenesis…….66 4.1.3 Effects of culture...

Ngày tải lên: 11/09/2015, 09:57

... Characterization of the transplanted vascular cells derived from human ES cells (HES-3) Characterization of the transplanted vascular cells derived from human ES cells (HES-3) A, Flow cytometric analysis of ... Figure cells differentiated from human of the vascular Schematic representation of preparationES cellstransplanted Schematic representation of preparation of the transplanted vascular cells differentiated ... composed of almost the same number of ECs and MCs We resorted the VE-cadherin+ cells from these expanded cells to obtain ECs for transplantation (Figure 1) The ECs derived from human ES cells (hES-ECs)...

Ngày tải lên: 18/06/2014, 15:20

... 1994) Derivation of human EG cells from aborted fetus has also been reported (Shamblott, Axelman et al 1998) The third type of stem cell is the adult stem cells Like ES and EG cells, adult stem cells ... Generation of Hepatocyte-like cells from Mouse Embryonic Stem (ES) and Bone Marrow (BM) Cells YIN YIJUN A THESIS SUBMITTED FOR THE DEGREE OF DOCTOR OF PHILOSOPHY OF MEDICINE DEPARTMENT OF OBSTETRICS ... Itskovitz-Eldor et al 1998; Reubinoff, Pera et al 2000) The second type of stem cell is embryonic germ (EG) cells isolated from the primordial germ cells of the gonadal ridge of the fetus (Matsui, Zsebo...

Ngày tải lên: 16/09/2015, 12:10

... classes of pluripotent stem cells in humans, other primates and mice These are embryonic stem cells, embryonic germ cells, embryonic carcinoma cells and recently the discovery of a fourth class of ... Adult stem cells Adult stem cells also known as somatic stem cells can be found in diverse tissues and organs The most common adult stem cells are the hematopoietic stem cells, mesechymal stem cells ... and neuronal stem cells Adult stem cells have also been isolated from several other organs such as the brain (neuronal stem cells) , skin (epidermal stem cells) , eye (retinal stem cells) and gut...

Ngày tải lên: 16/09/2015, 15:55

... VS, Jones JM Embryonic stem cell lines derived from human blastocysts Science 1998;282(5391):1145-7 Reubinoff BE, Pera MF, Fong CY, Trounson A, Bongso A Embryonic stem cell lines from human blastocysts: ... Bongso A An efficient and safe xeno-free cryopreservation method for the storage of human embryonic stem cells Stem Cells 2004;22(5):779-89 10 Speicher MR, Ballard SG, Ward DC Karyotyping human ... bioreactors Conflicts of interest The authors have declared that no conflict of interest exists References Becker EW The roles of ATP in the dynamics of the actin filaments of the cytoskeleton...

Ngày tải lên: 31/10/2012, 16:57

... activation of globin gene transcription during in vitro erythroid differentiation of murine embryonic stem cells PCR analysis of DNase I treated and reverse-transcribed total RNA extracted from differentiating ... the developmental progression of cell samples and in all cases except that of the Rex-1 gene, primer sets span introns Day cells are composed of ES cells and MEF cells grown in ES media containing ... Discussion B Fig Transcription of LCR hypersensitive site in undifferentiated murine embryonic stem cells and in human CD 133+ bone marrow cells (A) Transcription of LCR regions and the bmaj-globin...

Ngày tải lên: 07/03/2014, 12:20

... expression pattern of undifferentiated and differentiated human embryonic stem cells Stem Cells Dev 2007, 16(6):1003-1016 Page 15 of 17 (page number not for citation purposes) Journal of Translational ... green I6, H9 and BG01v are names of human embryonic stem (hES) cells lines P denoted the number of passages of the cell lines H9-EB denoted embryoid body (EB) prepared from cell line H9 and the day ... expression profiling We assessed global hES gene expression profiles on separate passages of cells from different hES cell lines, EB samples at different time points, and types of adult cells, HUVEC,...

Ngày tải lên: 18/06/2014, 15:20

... isolation of relatively pure populations of these cells for in vitro study of the cellular processes in thalassemic stem cells While previous attempts have focused on the cell culture system, in ... The isolated HSCs/CD34+ cells were harvested for phosphoproteomic analysis and cultivation of erythroid cells The cultivation of erythroid cells derived from HSCs/CD34+ cells was performed according ... culture of hematopoietic stem cells (HSCs) Bone marrow samples (BM) from Hb E/b-thalassemic patients and healthy donors were collected for HSCs isolation Briefly, mononuclear cells (MNCs) from BM...

Ngày tải lên: 18/06/2014, 19:20

... isolation of relatively pure populations of these cells for in vitro study of the cellular processes in thalassemic stem cells While previous attempts have focused on the cell culture system, in ... The isolated HSCs/CD34+ cells were harvested for phosphoproteomic analysis and cultivation of erythroid cells The cultivation of erythroid cells derived from HSCs/CD34+ cells was performed according ... culture of hematopoietic stem cells (HSCs) Bone marrow samples (BM) from Hb E/b-thalassemic patients and healthy donors were collected for HSCs isolation Briefly, mononuclear cells (MNCs) from BM...

Ngày tải lên: 20/06/2014, 04:20

... in mouse -5 embryonic stem cells using DCF-DA (10 M), a fluorescent probe of intracellular H2O2 Production of ROS was -3 detected in the cells exposed to H2O2 10 M for a variety of times (0-60 ... of nucleoside analogues with the sodium-nucleoside transport system in brush border membrane vesicles from human kidney Pharm Res 1993, Effect of dihydrotestosterone on mouse embryonic stem cells ... role of dihydrotestosterone in benign prostatic hyperplasia Urology 2003, 61 (Suppl 1), 2-7 16 Chambers I The molecular basis of pluripotency in mouse embryonic stem cells Cloning Stem Cells...

Ngày tải lên: 07/08/2014, 20:23

... access, cells from the IPFP are of interest as a potential source of cells for the repair of focal cartilage defects in the knee [5] In previous work we demonstrated the ability of IPFPderived cells ... day 14 The yield of cells by the end of passage was typically 107 cells at confluence from g of IPFP tissue (proliferation rate 0.14 ± 0.01 doublings per day (mean ± SEM) [8]) Cells at passage ... the autofluorescence (black) Chondrogenic culture of IPFP cells and the effect of low oxygen tension The cultured cell aggregates of IPFP cells with chondrogenic medium showed evidence of induction...

Ngày tải lên: 09/08/2014, 10:20

... oxygen depletion/reoxygenation conditions in mouse embryonic stem cells Methods Mouse embryonic stem cell culture The mouse embryonic stem (MES) cells (CCE-24) were routinely grown on 0.1% gelatin-coated ... http://www.jbiomedsci.com/content/18/1/46 Page of 10 Figure Confocal microscopy of a single colony of mouse embryonic stem cells A: top view of a single slice of scanning showing most eGFP signals are ... 5’-CAGCAATGCCTGGGTACATGGTG; Amplicon size: 249bp Results and Discussion Evidence of the existence of a smaller form of MsrA in mouse embryonic stem cells From our previous studies on MsrA[15], we have consistently...

Ngày tải lên: 10/08/2014, 05:21