derivation and propagation of embryonic stem cells in serum and feeder free culture pdf

Báo cáo y học: "Identification and isolation of embryonic stem cells in reproductive endocrinology: theoretical protocols for conservation of human embryos derived from in vitro fertilization" ppsx

Ngày tải lên : 13/08/2014, 23:20

... were obtained for further analysis Refinement and increased efficiency of these two ESC protocols brings the potential to offer a reliable supply of embryonic stem cells without production of whole ... The end of the beginning for pluripotent stem cells Nature 2001, 414:92-97 23 24 25 26 Doerflinger RM: The ethics of funding embryonic stem cell research: a Catholic viewpoint Kennedy Inst Ethics ... Study of Ethical Problems in Medicine and Biomedical and Behavioral Research: Defining death: a report on the medical, legal and ethical issues in the determination of death USGPO Washington...

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Báo cáo y học: " Curcumin mediated suppression of nuclear factor-κB promotes chondrogenic differentiation of mesenchymal stem cells in a high-density co-culture microenvironment" pdf

Ngày tải lên : 12/08/2014, 14:22

... inhibition of IκB kinase Page of 15 [13,18] There is increasing interest in curcumin as a therapeutic option for OA and RA, with evidence that curcumin inhibits the IL-1β-induced activation of ... demonstrate the influence of curcumin on the induction of the apoptotic signalling cascade by IL-1β in co-cultures, cultures were evaluated for activated caspase-3 and the marker of inflammation and prostaglandin ... ultrastructural findings described above and demonstrate that curcumin inhibits IL-1β induced apoptotic and inflammatory signalling pathways, promoting co -culture induced chondrogenesis Curcumin suppresses...

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báo cáo khoa học: " Embryonic stem cells in scaffold-free threedimensional cell culture: osteogenic differentiation and bone generation" ppt

Ngày tải lên : 11/08/2014, 20:21

... cultivation in the presence of osteoinductive stimuli Numerous living cells were detected in the mineralized centre of the spheres by means of toluidine blue staining (Figure 1) Generally, the mineralization ... prominent in the centre of the sphere, as demonstrated in histological sections stained with Figure Micromasses consisting of embryonic stem cells were cultured with or without DAG and stained ... the differentiation of stem cells into the osteogenic lineage in vitro J Bone Miner Res 2004, 19:1379-1394 Page of 38 Trounson A: Human embryonic stem cells: mother of all cell and tissue types...

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báo cáo khoa học:" Induction of osteogenic markers in differentially treated cultures of embryonic stem cells" ppt

Ngày tải lên : 12/08/2014, 01:22

... sources including autologous cells as well as allogenic and xenogenic cells [13-16] There are some reports that use totipotential embryonic stem cells in tissue engineering of bone [17,18] Embryonic ... staining (Fig 1) ESCs cultured in the presence of BMP-2 exhibited less mineralization, and there were no signs of mineral deposition in unstimulated control cells or cells stimulated with LIF In ... showed a prominent peak after days and a second peak beginning at day 17 The first increase was also seen in ESCs cultured in the presence of BMP-2 or the absence of LIF Interestingly, in all the...

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Báo cáo khoa học: Epigenetics: the study of embryonic stem cells by restriction landmark genomic scanning pptx

Ngày tải lên : 23/03/2014, 07:20

... establishment of embryonic stem (ES) cells, which maintain the ability to form all types of cells in the body, and can differentiate into a variety of cell types in vitro [8] The use of ES cells in epigenetic ... study of embryonic stem cells TE N Hattori and K Shiota ICM PGC TS cells Placental cells 77 ES cells 49 EG cells Embryonic cells Fig Epigenetic distances between ES cells and other stem cells ... Fig Involvement of Dnmts in establishing and maintaining DNAmethylation proﬁles Dnmt3a ⁄ 3b have an indispensable role for maintaining DNA methylation in gene areas, which are constituents of...

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differentiation of embryonic stem cells

Ngày tải lên : 11/04/2014, 00:32

... YAMAZAKI, AND SHIN-ICHI HAYASHI 98 v vi TABLE OF CONTENTS Development of Hematopoietic Repopulating Cells from Embryonic Stem Cells The In Vitro Differentiation of Mouse Embryonic Stem Cells into Neutrophils ... Development and Analysis of Megakaryocytes from Murine Embryonic Stem Cells 11 Development of Lymphoid Lineages Embryonic Stem Cells In Vitro from 12 Probing Dendritic Cell Function by Guiding the ... effects of gelatin, an impure mixture of matrix proteins derived from porcine or bovine skin, on the growth of pluripotent cells are often overlooked Gelatin can increase the differentiation of EPL cells...

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Báo cáo khoa học: " Effect of dihydrotestosterone on mouse embryonic stem cells exposed to H2O2-induced oxidative stress" pdf

Ngày tải lên : 07/08/2014, 20:23

... was loaded into a 96-well plate and examined using a luminometer (Victor3; PerkinElmer, Finland) and a fluorescent plate reader with excitation and emission wavelengths of 485 nm and 535 nm, ... We determined the H2O2-induced ROS generation in mouse -5 embryonic stem cells using DCF-DA (10 M), a fluorescent probe of intracellular H2O2 Production of ROS was -3 detected in the cells exposed ... o culture dish in an incubator maintained at 37 C in an atmosphere containing 5% CO2 and air Measurement of lipid peroxides The cells were pretreated with or without DHT for 30 and then were treated...

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The signals of FGFs on the neurogenesis of embryonic stem cells ppsx

Ngày tải lên : 10/08/2014, 05:21

... 2C) RT-PCR and immunostaining, shown in Figs 2B and 2C, indicated that the expression of FGFR2 in differentiating ES cells was robustly induced and was localized on the cell membrane and cytosol, ... concentration of 80 ng/ml, failed to enhance the neural induction of ES cells (Fig 2A) We further investigated the expression of FGFRs in ES cells during neural induction and found that the expression of ... Nuclei of all cells are revealed by DAPI staining in blue Scale bar, 10 μm in C *, p < 0.01, Anova test into neuroblast cells Immunocytostaining revealed that both FGFR1 and FGFR3 were detected in...

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báo cáo khoa học: " Efficacy of Mesenchymal Stem Cells in Suppression of Hepatocarcinorigenesis in Rats: Possible Role of Wnt Signaling" doc

Ngày tải lên : 10/08/2014, 10:21

... of MSCs to cancer cells, nature of tumour cells and cancer stem cells, integrity of immune system, number of stem cell passages and site of injection; all can affect the outcome of MSCs use in ... the induction of parenchymal damage is a prerequisite for successful homing and repopulation with stem cells [39,40] Molecular mechanisms underlying stem cells mobilization and homing into the injured ... that maintain and regulate stem cells [52], Livraghi and colleagues [53] stated that the essential role of stem cell microenvironment in preventing carcinogenesis is by providing signals to inhibit...

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The derivation, propagation, storage and gene expression of human embryonic stem cells on human feeders

Ngày tải lên : 16/09/2015, 15:55

... manipulation of stem cells 35 CHAPTER Derivation and Propagation of Human Embryonic Stem Cell Lines on Xeno -Free Support Systems and in the Presence of Xeno -Free Proteins 36 Introduction hESC lines have ... brain (neuronal stem cells) , skin (epidermal stem cells) , eye (retinal stem cells) and gut (intestinal crypt stem cells) (Spradling et al 2001) However, 15 not all organs and tissues may contain ... involving blastocyst injections and ectopic grafting in adult hosts cannot be performed in the human Animal embryonic stem cells Embryonic stem cells were first derived from certain strains of...

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báo cáo hóa học:" MicroRNA and gene expression patterns in the differentiation of human embryonic stem cells" doc

Ngày tải lên : 18/06/2014, 15:20

... expression in embryonic stem cells suggest they are involved in maintaining "stemness" Identifying mRNAs that are directly targeted by a specific miRNA is a major obstacle in understanding the miRNA ... greater in hES cells than in EB cells and it was not even detected in adult cells The expression of miR-520b was 26-fold greater in hES cells then in EB cells (P < 0.05) and it was detected only in ... names of human embryonic stem (hES) cells lines P denoted the number of passages of the cell lines H9-EB denoted embryoid body (EB) prepared from cell line H9 and the day indicates the time in culture...

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Differentiation and derivation of lineage committed chondroprogenitors and chondrogenic cells from human embryonic stem cells for cartilage tissue engineering and regeneration

Ngày tải lên : 11/09/2015, 09:57

... systems that include human feeder systems, complete feeder- free and xeno -free systems Richards et al., 2002 reported the first establishment of human feeder supported culture of hESCs in xeno -free medium ... Immunofluorescence (IF) staining………………………………… 53 3.7.2 Staining of tissue specimens……………………………………… 54 3.7.2.1 Processing of tissue specimens…………………………………… 54 3.7.2.2 Haematoxylin and eosin staining………………………………… ... Cartilage tissue engineering using hydrogels Cartilage tissue engineering using hydrogels enables the delivery of cells and bioactive agents, including growth factors, peptides and ligands Hydrogels...

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Role of sonic hedgehog signalling in human embryonic stem cells and its neural derivatives

Ngày tải lên : 11/09/2015, 10:14

... Immunoflourescent staining of neural stem cell marker Nestin in SHH-CM and Control-CM treated EB Middle panel shows corresponding DAPI nuclear staining in blue and right panel shows corresponding ... forebrain, midbrain, hindbrain and the spinal cord This figure was reproduced from Epstein et al., 1999 2.4 SHH and neural development During the initial phase of neural induction, the ectoderm is induced ... (Ben-Nun and Benvenisty, 2006; Dvash et al., 2006) 2.7 Embryonic stem cells and induced pluripotent stem cells Embryonic stem cells (ESC) have two characteristics that distinguish them from other stem...

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Identification of oct4 and sox2 targets in mouse embryonic stem cells

Ngày tải lên : 11/09/2015, 16:03

... genome binding sites of Oct4 and Sox2 Thousands of novel Oct4 and Sox2 binding sites were identified, mapping to genes implicated in important cellular functions including maintenance of pluripotency ... Oct4 and Sox2, which are two key transcription factors that are essential in maintaining pluripotency of ESC In order to gain a deeper understanding of the functions of Oct4 and Sox2 in governing ... Genome-wide Mapping of Oct4-DNA Interactions in Mouse Embryonic Stem Cells 4.1 Introduction 74 4.2 Results 76 4.2.1 Optimisation of large-scale ChIP 76 4.2.2 Global mapping of Oct4 binding sites in mESCs...

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Báo cáo khoa học: Recruitment of transcription complexes to the b-globin locus control region and transcription of hypersensitive site 3 prior to erythroid differentiation of murine embryonic stem cells docx

Ngày tải lên : 07/03/2014, 12:20

... could maintain a small accessible region in the globin locus during the differentiation of hematopoietic stem cells to erythroid cells Transcription of HS3 could be important in maintaining this ... differentiation of murine embryonic stem cells PCR analysis of DNase I treated and reverse-transcribed total RNA extracted from differentiating embryonic stem cells at the indicated time points All ... chromatin structure modiﬁcations and factor recruitment at the murine b-globin locus in uninduced embryonic stem cells (day 0), as well as that of primitive and deﬁnitive erythroid cells (days and...

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báo cáo hóa học:" Transplantation of vascular cells derived from human embryonic stem cells contributes to vascular regeneration after stroke in mice" docx

Ngày tải lên : 18/06/2014, 15:20

... characterization of transplanted cells derived from human ES cells We induced differentiation of human ES cells in an in vitro two-dimensional culture on OP9 stromal cell line and examined the expression of ... Translational Medicine 2008, 6:54 cells, the number (cells/ mm2/mouse) of single stranded DNA (ss-DNA)+ cells in one field in the ischemic core from each mouse in the saline- or hES-ECs+MCs-injected group ... Biosciecnces, 1:100), Neu-N (Chemicon, 1:200), and single stranded DNA (Dako Cytomation, 1:100) Immunohistochemical examination of cultured cells Staining of cultured cells on dishes at 5th passage was performed...

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báo cáo hóa học:" Implantation of neural stem cells embedded in hyaluronic acid and collagen composite conduit promotes regeneration in a rabbit facial nerve injury model" doc

Ngày tải lên : 18/06/2014, 15:20

... staining of β-tubulin, glial fibrillary acidic protein (GFAP) and galactocerebroside (GalC) in neurosphere-derived cells cultured on poly-L-ornithine- and laminin-coated coverslips displaying ... staining of BrdU and S-100 Representative images of immunohistochemical staining of BrdU and S-100 A: Localization of darkly brownish stained BrdU+ cells to transected tissues of rabbits having ... for three days in 4% paraformaldehyde and embedded in paraffin Sections were de-waxed and stained with haematoxylin and eosin for histological examination Toluidine blue staining was performed...

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Báo cáo hóa học: " Nutraceutical augmentation of circulating endothelial progenitor cells and hematopoietic stem cells in human subjects" potx

Ngày tải lên : 18/06/2014, 16:20

... a peak increase of approximately 53% in the number of CD34 expressing cells and and a 90% increase in CD133 cells in circulation Furthermore, a significant augmentation of cells possessing hematopoietic ... required to ingest two capsules of Stem- Kine in the morning and two in the evening for 14 days Phenotypic assessment of circulating stem cells Peripheral blood mononuclear cells (PBMC) were isolated ... marrow injury conditions [43], subsequent studies have expanded this finding to situations of inflammation [44], and peripheral tissue injury [45-47] Hematopoietic stem cells are being increasingly...

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Báo cáo khoa học: "In vitro neuronal and osteogenic differentiation of mesenchymal stem cells from human umbilical cord blood" ppt

Ngày tải lên : 07/08/2014, 18:21

... llec gnimrofrep dna esu lacinilc rof SBF gnisu tuohtiw oviv xe sCSM dnapxe ot sdohtem dnif ot deen ew dna ,hcraeser ruo rof SBF desu ew ,yduts tneserp ni ,revewoH noitarefilorp sCSM rof noitidnoc ... 001-X notirT %4.0 neht dna ,nim rof enoteca %001 ,nim 01 rof lonahtem %001 dloc eci htiw detaert neht erew yehT erutarepmet moor ta nim 01 rof SBP ni edyhedlamrof %7.3 htiw dexif neht dna SBP ... noitcudni cinegoetso na desu ew ,sCSM eht morf noitaitnereffid cinegoetso ecudni oT laitnetop cinegoetso tibihxe sCSM doolb droC )A2 giF( nitnemiv dna 2HS snegitna detaler -myhcnesem rof ytivitisop...

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Báo cáo khoa học: "Functional recovery and neural differentiation after transplantation of allogenic adipose-derived stem cells in a canine model of acute spinal cord injury" potx

Ngày tải lên : 07/08/2014, 23:22

... hematoxylin and eosin, and then with combined Luxol fast blue and cresyl violet to identify myelin and nerve cells [10] Percentages of myelinated areas in damaged spinal cords were calculated using ... B and C: H&E stain, D-G: Luxol fast blue and cresyl violet stain, H, H1, H2 and H3: Immunofluorescence staining Scale bars = 50 μm Transplantation of ASCs in spinal cord injury 281 Fig Immunofluorescence ... stained with Alizarin red S D: Intense Alizarin red S stained after weeks incubation at osteogenic media and confirmed calcium deposition A and B: Oil red O stain, C and D: Alizarin red S stain,...

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