... transplantation of both female PGCs and embryonic gonadal somatic cells underneath the ovarian bursa or the kidney capsule of recipient mice resulted in the transformation of induced embryonic stem cells ... week Of these, 4.65% of the cells were positive for the germ cell marker Vasa and some of the cells were also positive for additional germ and stem cell markers, such as Fragilis, Thy-1, SSEA4 and ... possible to derive and expand autologous stem cells from ovarian tissue The isolation and characterization of human PSCs will contribute considerably to the prospect of using stem cells to produce
Ngày tải lên: 16/12/2017, 04:38
... fibroblasts and human primary fibroblasts and epithelial cells and microvascular endothelial cells and primary melanocytes survive and proliferate in the setting of PKCδ knockdown or in concentrations of ... self-renewing and anchorage-independent spheroids were obtained by culturing breast cancer cells MCF7, Hs587T and MDA231; melanoma cells SBcl2 and FM6; human breast cancer stem cells and pancreatic ... the means of two sets of sample data and considered significant if p ≤ 0.05 Results Inhibition of PKCδ is growth-inhibitory and cytotoxic in human prostate and pancreatic cancer stem cells The
Ngày tải lên: 05/11/2020, 01:11
Identification and characterization of cancer stem cells in human head and neck squamous cell carcinoma
... initiation, growth, and invasion of cancer are driven by a small population of cancer stem cells (CSC) Previous studies have identified CD44+ cells as cancer stem cells in head and neck squamous ... Nanog Figure Expression of CD24 and CD44 in A253 HNSCC cells (A) Flow cytometric analysis of CD24+ and CD44+ cells in A253 HNSCC cell line Dual staining of A253 HNSCC cells indicate that CD24+/CD44+ ... removed from the upper surface of the membrane with a cotton swab, and cells on the bottom surface of the membrane were fixed and stained with H&E Cells in five random fields per well were counted
Ngày tải lên: 05/11/2020, 01:35
identification and characterization of cancer stem cells in human head and neck squamous cell carcinoma
... initiation, growth, and invasion of cancer are driven by a small population of cancer stem cells (CSC) Previous studies have identified CD44+ cells as cancer stem cells in head and neck squamous ... Nanog Figure Expression of CD24 and CD44 in A253 HNSCC cells (A) Flow cytometric analysis of CD24+ and CD44+ cells in A253 HNSCC cell line Dual staining of A253 HNSCC cells indicate that CD24+/CD44+ ... removed from the upper surface of the membrane with a cotton swab, and cells on the bottom surface of the membrane were fixed and stained with H&E Cells in five random fields per well were counted
Ngày tải lên: 02/11/2022, 11:41
mesenchymal morphogenesis of embryonic stem cells dynamically modulates the biophysical microtissue niche
... & Zandstra, P W Analysis of the temporal and concentration-dependent effects of BMP-4, VEGF, and TPO on development of www.nature.com/scientificreports embryonic stem cell-derived mesoderm and ... dysregulation of niche elements leads to differentiation and migration of resident stem cell populations5 Therefore, the synergy of biophysical and biochemical signals and the complexity of the local ... Stem cells and niches: mechanisms that promote stem cell maintenance throughout life Cell 132, 598–611 (2008) Fuchs, E., Tumbar, T & Guasch, G Socializing with the neighbors: stem cells and their
Ngày tải lên: 02/11/2022, 14:35
OCT 3 4 and SOX 2 are key factors for SDIA neurogenesis of mouse embryonic stem cells
... knockdown in mouse embryonic stem cells Biotechnol Lett 8:8 Chen S, Choo A, Wang ND, Too HP, Oh SK 2006 Knockdown of Oct4 and Sox2 Attenuate Neurogenesis of Mouse Embryonic Stem Cells Stem Cells Dev (accepted) ... pluripotent embryonic stem cells and extra-embryonic cells Embo J 17:2019-2032 Okumura-Nakanishi S, Saito M, Niwa H, Ishikawa F 2005 Oct-3/4 and Sox2 regulate Oct-3/4 gene in embryonic stem cells J ... DC, Sutherland L, Clark J, Burdon T 2004 Oct-4 knockdown induces similar patterns of endoderm and trophoblast differentiation markers in human and mouse embryonic stem cells Stem Cells 22:225-235
Ngày tải lên: 27/11/2015, 11:25
Directed differentiation of human embryonic stem cells into haematopoietic and definitive endodermal lineages
... endoderm differentiation of hESCs Table 5.1 Available information on knock-out phenotypes in the mouse vii LIST OF FIGURES Figure 1.1 Embryonic origin of human embryonic stem cells and their in vitro ... to my life and my world Pappa and Mamma, thank you for your love and unconditional support My grandparents and the rest of my extended family have been encouraging me whole-heartedly and I sincerely ... DIFFERENTIATION OF HUMAN EMBRYONIC STEM CELLS INTO HAEMATOPOIETIC AND DEFINITIVE ENDODERMAL LINEAGES ABRAHAM SUMAN MARY (M.Sc MICROBIOLOGY, UNIV OF MUMBAI, INDIA) A THESIS SUBMITTED FOR THE DEGREE OF MASTER
Ngày tải lên: 04/10/2015, 16:03
báo cáo hóa học:" Transplantation of vascular cells derived from human embryonic stem cells contributes to vascular regeneration after stroke in mice" docx
... type (VEGF-R2)-positive cells induced from mouse embryonic stem (ES) cells can differentiate into both endothelial cells (ECs) and mural cells (MCs) and these vascular cells construct blood vessel ... expanded cells as a mixture of ECs and MCs derived from human ES cells (hESECs+MCs) The cell mixture was composed of almost the same number of ECs and MCs We resorted the VE-cadherin+ cells from ... VEGF-R2-positive cells induced from undifferentiated mouse embryonic stem (ES) cells can differentiate into both VE-cadherin-positive endothelial cells (ECs) and αSMA-positive mural cells (MCs), and these
Ngày tải lên: 18/06/2014, 15:20
Báo cáo hóa học: "Future research and therapeutic applications of human stem cells: general, regulatory, and bioethical aspects" ppt
... purposes, human embryonic stem cells are used in 13% of cell therapy procedur es, while fetal stem cells are used in 2%, umbilical cord stem cells in 10%, and adult stem cells in 75% of treatments. ... adult progenitor cells (MAPCs), are the stem cells forming bone marrow [33]. Their role is maintenance and turnover of blood cells and immune system. The high rate of regeneration of the liver, ... functional repair of myocardial infarction, and in stroke [31,32]. Other types of stem cells Hematopoietic stem cells together with mesenchymal stem cells, the so-called “side population”, and multipo-
Ngày tải lên: 18/06/2014, 16:20
Báo cáo khoa học: "Human embryonic stem cells and therapeutic cloning" pdf
... PJ, Andrews PW. Culture and characterization of human embryonic stem cells. Stem Cells Dev 2004, 13, 325-336 18. Drukker M, Benvenisty N. The immunogenicity of human embryonic stem-derived cells. ... review, we describe the derivation and characterization of ES cells and cloned ES cells. Furthermore, current perspectives of potential applications of stem cells for tissue repair and transplantation ... Kishimoto T. Maintenance of the pluripotential phenotype of embryonic stem cells through direct activation of gp130 signalling pathways. Mech Dev 1994, 45, 163-171. 87. Zerhouni E. Embryonic stem cells.
Ngày tải lên: 07/08/2014, 18:21
Báo cáo y học: "Identification of a truncated form of methionine sulfoxide reductase a expressed in mouse embryonic stem cells" pps
... mutagenesis studies of Kim and Gladyshev [18], the deletion is limited only to the very end of the N- Figure 5 Confocal microscopy of a single colony of mouse embryonic stem cells. A: top view of a single ... collection and assembly of data, data analysis and interpretatio n and manuscript writing for this paper. CZ participated in the conception and design, collection and assembly of data, data analysis and ... 5’-CAGCAATGCCTGGGTACATGGTG; Amplicon size: 249bp. Results and Discussion Evidence of the existence of a smaller form of MsrA in mouse embryonic stem cells From our previous studies on MsrA[15], we have
Ngày tải lên: 10/08/2014, 05:21
báo cáo khoa học: " Transcriptomic analysis of pluripotent stem cells: insights into health and disease" potx
... Abstract Embryonic stem cells (ESCs) and induced pluripotent stem cells (iPSCs) hold tremendous clinical potential because of their ability to self-renew, and to differentiate into all cell types of ... transcriptomics Stem cell transcriptomics and transcriptional networks Embryonic stem cells (ESCs) have the unique ability to self-renew and differentiate into cells of all three germ layers of the body ... Feinberg AP: Differential methylation of tissue- and cancer-specific CpG island shores distinguishes human induced pluripotent stem cells, embryonic stem cells and fibroblasts Nat Genet 2009, 41:1350-1353
Ngày tải lên: 11/08/2014, 12:21
Báo cáo y học: "The impact of chromatin modifiers on the timing of locus replication in mouse embryonic stem cells" pot
... relationship between chromatin of mutant embryonic stem (ES) A panelof replication timing in ES cells cell Regulationstructure and replication cells. lines the importance of chromatin modifiers was ... values and standard error of at least two independent experiments are shown (b,c) Summary of replication timing of candidate genes (b) and loci surrounding the Rex1 gene (c) In (c), positions of ... retrotransposons (LINE and SINE B1) and rDNA repeats in wild-type OS25 ES cells and in mutant ES cells lacking Eed, Dnmt 1, Dnmt 3a/3b, G9a or Dicer The mean values and standard error of at least two
Ngày tải lên: 14/08/2014, 08:20
Identification of factors involved in the maintenance of embryonic stem cell self renewal and pluripotency 2
... seeding densities of 300 and 500 cells per 56cm2 (surface area of each well of a well dish is 56cm2) When 300 cells were reseeded, the number of secondary colonies formed for cells transfected ... compare the extent of differentiation between Lefty2 RNAi cells and control cells a) Two seeding densities (300 and 500 cells) were performed for one of the shRNAs while b) 300 cells were replated ... transcriptome profiling by Wei et al (2005) using MPSS also highlighted a list of genes with differential expression patterns between ES cells and differentiated cells and these may be prime candidates...
Ngày tải lên: 11/09/2015, 16:02
Identification of factors involved in the maintenance of embryonic stem cell self renewal and pluripotency 4
... ratios of 2µg shRNA:3µg CAG vectors or 2µg shRNA:4µg CAG vector A ratio of 1µg DNA: 0.5µl Lipofectamine2000 was used for transfection Dual drug selection of 1µg/ml of puromycin and 300µg/ml of Gentamycin ... prepare two more batches of conditioned medium and then discarded For trophoblast stem cell derivation, E14 cells were first seeded on 12 well plates at a density of 3000 per well and incubated in growth ... morphology and staining and the numbers of secondary colonies were counted for all samples and compared 4.11 RNA isolation, reverse transcription (cDNA synthesis) and real-time PCR analysis Cells...
Ngày tải lên: 11/09/2015, 16:02
Identification of factors involved in the maintenance of embryonic stem cell self renewal and pluripotency
... 1.1 Embryonic stem cells 1.1.1 Derivation and definition of embryonic stem cells During the transition of an embryo from the morula to the blastocyst ... into its extraembryonic and embryonic components The outer layer of cells of the blastocyst is the extraembryonic components and forms an epithelium, the trophectoderm Descendents of the trophectoderm ... differentiation 17 Two landmark papers published recently have elucidated the binding landscape of OCT4 and NANOG in mouse ES cells and OCT4, NANOG and SOX2 in human ES cells (Boyer et al., 2005;...
Ngày tải lên: 11/09/2015, 16:02
Appendix identification of factors involved in the maintenance of embryonic stem cell self renewal and pluripotency
... ES cells and ICM Differential expression pattern between ES cells and differentiated cells Co-expressed with ES transcription factor like Nanog during mouse preimplantation development Oct4 and ... target in mouse Es cells LeftyB (human Lefty1) is regulated by Wnt pathway High expression in ES cells and ICM Differential expression pattern between ES cells and differentiated cells Co-expressed ... Sox 2and Nanog target in human ES cells LeftyA (human Lefty2) is regulated by Wnt pathway Expression detected in ES cells and F9 murine teratocarcinoma Differential expression pattern between ES cells...
Ngày tải lên: 11/09/2015, 16:03
differentiation of embryonic stem cells
... Mouse Embryonic Stem Cells YIJING CHEN, JAY L VIVIAN, AND TERRY MAGNUSON 406 Section IV Differentiation of Monkey and Human Embryonic Stem Cells 29 Growth and Differentiation Monkey ES Cells of Cynomolgus ... from Murine Embryonic Stem Cells 11 Development of Lymphoid Lineages Embryonic Stem Cells In Vitro from 12 Probing Dendritic Cell Function by Guiding the Differentiation of Embryonic Stem Cells 13 ... CONTENTS Development of Hematopoietic Repopulating Cells from Embryonic Stem Cells The In Vitro Differentiation of Mouse Embryonic Stem Cells into Neutrophils 10 Development and Analysis of Megakaryocytes...
Ngày tải lên: 11/04/2014, 00:32
Báo cáo y học: "An in vitro study investigating the survival and phenotype of mesenchymal stem cells following injection into nucleus pulposus tissue" pot
... source are adult stem cells, in particular, bone-marrow-derived mesenchymal stem cells (MSCs) The use of these cells would allow an autologous approach, reducing the risk of rejection and infection ... field of view was performed to enable identification of GFP-positive cells and immunopositivity for matrix proteins in the same cells Results Identification of injected mesenchymal stem cells ... identify the presence of apoptotic cells; for immunofluorescence and immunohistochemistry for aggrecan, Sox-9 and types I, II and X collagen to assess phenotypic characteristics; and for histochemistry...
Ngày tải lên: 09/08/2014, 01:22
The signals of FGFs on the neurogenesis of embryonic stem cells ppsx
... Sox1-GFP+ cells from day to day (Fig 3A) The number of Sox1+ cells became 20% of total cells on day and reached the plateau, 50% of total cells, on day Treatment of FGF1 consistently and dose-dependently ... and/ or reduced apoptosis of neuroblast cells To test these possibilities, FGF1 was incubated with the 46C cells, and the apoptosis and proliferation of Sox1+ cells were analyzed by staining of ... promotes self-renewal of mouse embryonic stem cells Dev Biol 1999, 210:30-43 Li Z, Theus MH, Wei L: Role of ERK 1/2 signaling in neuronal differentiation of cultured embryonic stem cells Dev Growth...
Ngày tải lên: 10/08/2014, 05:21