dependent turbulent mixing and chemical reaction in stirred tanks1

... blood cells and pieces of tissue containing insufficient fat cells to float Fat and nonfat cells were isolated by incubating 1.0 g of cut adipose tissue in ml of incubation medium containing 1.3 ... (Bovuminar, containing

Ngày tải lên: 11/08/2014, 08:22

... in high glucose DMEM supplemented with 10% heatinactivated FCS, 1% glutamine, 50 U/ml penicillin and Page of 50 μg/ml streptomycin and incubated at 37°C with 100% relative humidity in a CO2 incubator ... (USA) for 10 minutes, and the supernatant containing 50 μg of protein was incubated with 50 μM Ac-DEVD-AMC (Calbiochem, USA) at 37°C for 30 minutes The release of 7-amino-4methylcoumarin (AMC) was ... HEt-staining fluorescent imaging technology As shown in Figure 6, hypoxia-reoxygenation treatment markedly induced the HEt fluorescence, indicating the production of O2- Both EGb761 and quercetin...

Ngày tải lên: 13/08/2014, 14:20

... building] scleractinian corals in a coral reef fishery)”; such activities include chemical and blast fishing, anchor damage, trawl fishing, fishing with fine mesh gill nets, and “weighted scare lines” ... Fishing Practices In a comparison of the two destructive fishing methods chemical and blast fishing, Pet-Soede and Erdmann (1998) observed that chemical fishers in Indonesia are “quite sparing in ... advice in the research and writing: Gene Ammarell and James K Lein from Ohio University and M Iqbal Djawad from Hasanuddin University in Indonesia Many thanks to Jamaluddin Jompa of Hasanuddin University...

Ngày tải lên: 14/05/2015, 12:10

... HF + H reaction is one of the most studied chemical reactions in science, and interest in this reaction dates back to the discovery of the chemical laser.79 In the early 1970s, a collinear quantum ... topics in the chemical dynamics field Lee and Liu discusses in Chapter 1, a three-dimensional velocity mapping approach to study dynamics in elementary chemical reactions In Chapter 2, Chao and Skodje ... involving interesting avoided crossings as well as conical intersections can now be studied using both quantum chemical and dynamical methods Dynamics of larger systems such as large clusters and...

Ngày tải lên: 01/04/2014, 10:35

... eliminated Figure Comparison of sugar concentrations from soaking and no soaking process: (beside soaking and no soaking) represented glucose concentration and (beside soaking and no soaking) ... Chemist Soceity and Institute of Food Technology She is a current Ph.D candidate Yaser Dahman holds a Ph.D in Chemical and Biochemical Engineering from the University of Western Ontario and an MBA ... illustrated glucose and xylose concentrations during soaking and no soaking process Soaking process produced the same glucose and xylose concentrations as the no soaking process at all time intervals This...

Ngày tải lên: 05/09/2013, 15:28

... protein was decreased due to formation of cisplatin adducts within the p53 DNA-binding site (p53DBS), resulting in increased p53 binding to the labeled probe The pPGM4 fragment (c) contains cisplatinresistant ... lacking the cisplatin-reactive motifs or containing multiple guanine doublets and ⁄ or triplets (Fig 7), were used to check the in uence of cisplatin adducts in the vicinity of p53DBS Again, ... affected by DNA cis-platination in a similar way to p53 binding In agreement with the considerable homology between the p53 and p73 DNA-binding (core) domains, the p73 protein can bind to the p53 response...

Ngày tải lên: 19/02/2014, 05:20

... tagged protein had a similar activity in crude yeast extract as the untagged protein, indicating that the tag was not interfering with the protein activity The tagged protein was purified and then ... with a histidine tag at the N-terminal end by adding the coding sequence for six histidines to the end of the open reading frame, and then expressed in S cerevisiae The tagged protein had a similar ... methylglyoxal and diacetyl In the oxidative reaction, the enzyme was active with glycerol and to a lower Table The specificities and kinetic properties of the histidine-tagged and purified GLD1 and GLD2...

Ngày tải lên: 19/02/2014, 06:20

... found in the indoor environment, including in air and dust (Otake et al 2001, Butte & Heinzow 2002, Fromme et al 2004) Phthalates are commonly found in human tissues, including in blood and, as ... additives in plastics and foams, including plastic casings of electronic equipment (OECD 2003) There are many different chemicals (congeners) included in this group, differing in the numbers and positioning ... Socolof 2005) and within the glass as lead oxide (ICER 2003), and the use of zinc compounds in ‘phosphor’ coatings (Burstall 1997) Under landfill conditions, metals including lead and zinc are able...

Ngày tải lên: 05/03/2014, 21:20

... other known cysteine-containing chitin-binding domains [14], such as plant chitin-binding proteins and horseshoe crab antimicrobial peptides with chitin-binding affinity [15–17] Rebers and Willis reported ... chitin-binding proteins in T tridentatus Amino acid compositions of high molecular mass (HMM) chitin-binding proteins HMM chitin-binding proteins resolved by 2D SDS ⁄ PAGE were categorized into ... with asterisks Numbers on the right indicate amino acid residue numbers and DE29 is involved in chitin binding In insects, cuticular proteins containing cysteine residues have not been reported,...

Ngày tải lên: 07/03/2014, 21:20

... before and after germination * p < 0.05 ** p < 0.01 *** p < 0.001 soluble vitamins, such as vitamin A, vitamin E diminish the lipid content of blood plasma in patients with non-insulin -dependent ... Fructose and sucrose were found before germination, and fructose and glucose found after germination Total free sugar content increased after germination Glucose which was absent in rough rice seed and ... different seed parts before and after germination are shown in Table Palmitic, oleic, and linoleic acids were the major fatty acids (80%), and stearic and linolenic acids were minor fatty acids The...

Ngày tải lên: 15/03/2014, 15:42

... great interest both from an environmental (geochemical) and an industrial point of view Most solid phases in natural water contain aluminium oxides Alumina plays an important role in regulating ... alumina and other mineral solids in the presence of other complexing agents such as nitrilotriacetic acid and ethylenediaminetetraacetic acid has also been studied and was found to increase in ... solution, including surface charging and sorptive capacity, are determined by the nature of their surface functional groups, the ability of these groups to bind protons and adions, and the bonding...

Ngày tải lên: 15/03/2014, 23:22

... Statistical Bureau and P.R China, 2000) With continuing population growth and rapid economic development of China, the percentage of population living in cities is increasing dramatically and, consequently, ... determine the total Cu, Zn, Pb and Cr concentrations and chemical speciation of urban soils in Nanjing, China Materials and methods 2.1 Description of study area Nanjing city is located in eastern ... increased with the increase of clay content (Xu and Yang, 1995; Liu, 1996) Lu (2000) reported that Cu, Zn, Pb and Cr in Nanjing urban soils were mainly originated from anthropogentic input, and...

Ngày tải lên: 15/03/2014, 23:22

... protein unfolding, many aromatic amino acids forming the protein core become exposed with the concomitant increase in the molar extinction coefficient of the protein, and thus the increase in absorbance ... protein is shown in Fig 3A indicating good dispersion of the backbone and side-chain signals (the latter including those assigned to tryptophane indole groups in the 5594 Fig Structure determination ... CD69 in buffers with neutral or slightly alkaline pH under conditions compatible with binding of potential ligands We succeeded in crystallizing the very stable CD69NG70 protein using l-arginine...

Ngày tải lên: 16/03/2014, 04:20

... alone and a completely annealed reaction Samples were taken at 0, 1, 3, 10, and 30 Single-stranded (ss) and double-stranded (ds) species are indicated (B) Bar chart representing annealing percentage ... obtained at 30 (means ± SEM, n ¼ 3) **Indicates P < 0.01 in respect of controls (one way ANOVA, Tuckey test) protein that binds single-stranded nucleic acids and is able to promote interstrand ... GST-CNBP phosphorylation in the presence of heparin, a highly selective and potent inhibitor of CK2 [30] In several independent experiments, no significant heparin phosphorylation inhibition was observed...

Ngày tải lên: 16/03/2014, 12:20

... motifs in domains and ( 70% identical residues), particularly in the DNA-binding region 732 (domain 1) which contains an HTH motif [21] and the metal-binding and dimerization domains (domain 2) ... ferritin regulation in Streptomyces species In addition to the 11 proteins listed in Tables and 2, minor changes in other proteins were observed These proteins may be involved in other reactions ... al Two iron -dependent regulators in S coelicolor Fig Comparative alignment of domains (DNA–protein interaction), (dimerization and metal binding) and (containing a nonconserved amino acid stretch),...

Ngày tải lên: 16/03/2014, 18:20

... vinyl textured white vinyl finish with finish with unfinished veneer unfinished veneer backing backing vinyl laminant with vinyl laminant with unfinished veneer unfinished veneer backing backing ... Coverings and Finishes on Tested Materials Material ceiling wall sub floor carpet vinyl floor cabinet wall cabinet wall thick Dutchmen textured white vinyl finish with unfinished veneer backing vinyl ... materials using bonding, coating, or surface finishing systems containing formaldehyde when installed in manufactured homes (Turner et al 1996) The standard is embodied in the HUD Standard 24 CFR...

Ngày tải lên: 22/03/2014, 14:20

... protein, calculated as (I ) Imin) ⁄ (Imax ) Imin); I is the fluorescence intensity at a given temperature, and Imax and Imin are the maximum and minimum values of fluorescence intensity corresponding ... Crescenzi O, Tancredi T & Picone D (2003) Role of the hinge peptide and the intersubunit interface in the swapping of N-termini in dimeric bovine seminal RNase Eur J Biochem 270, 4729–4735 Leich F, ... protein was calculated as (Q ) Qmin) ⁄ (Qmax ) Qmin); Q is the ellipticity at 222 nm at a given temperature, and Qmax and Qmin are the maximum and minimum values of ellipticity corresponding to...

Ngày tải lên: 22/03/2014, 17:20

... mechanism in protein folding and chemical reactions Experimental Section Protein Engineering and Purification: We constructed an eight domain N-C linked polyprotein of I27, the 27th immunoglobulinlike ... conditions, stretching a polyprotein results in a well-defined series of step increases in length, marking the unfolding and extension of the individual modules in the chain.[37] The size of ... molecules bridging the gap between separating b-strands In Figure B, we define the pulling coordinate for the A’ and G b-strands as the distance between the first amino acid of strand A’ (Y9) and the...

Ngày tải lên: 22/03/2014, 18:20

... Translation initiation in mammals and yeast is well studied; it involves many RNA–RNA, protein–RNA, and protein–protein interactions In contrast, knowledge about the process of protein synthesis in Trypanosomatidae ... into the cytosol of L donovani infected macrophages and inactivate them [74] EF-1a plays an important role in eukaryotic protein biosynthesis by binding aminoacyl-tRNAs and positioning them in ... functional interaction between eIF4A and LeIF with eIF4G The recombinant LeIF protein exhibits poor ATPdependent duplex unwinding activity in vitro as shown previously for eIF4A [30] The unwinding in...

Ngày tải lên: 23/03/2014, 10:20

... serine and serine 12 in vivo and by casein kinase I J Biol Chem 268, 26712–26718 36 Cowles, E.A., Agrwal, N., Anderson, R.L & Wang, J.L (1990) Carbohydrate-binding protein 35 Isoelectric points ... switch serines 73 and 138 and threonine 153 of the HIP/PAP protein for alanines Oligonucleotides cassettes containing the desired mutations were inserted into pcDNA3-HIP/PAPmyc-His as indicated ... cytokine that combines mitogenic and anti-apoptotic functions using pathways involving PKA [16] In conclusion, our findings lead us to propose PKA as a target for HIP/PAP, a C-type lectin and thus...

Ngày tải lên: 23/03/2014, 13:20