deoxyribonucleic acid dna makes ribonucleic acid rna 7

... mRNA: c phiờn t si antisense strand Phõn loi RNA (Da vo v trớ v chc nng m nhn) mRNA (messenger RNA) tRNA (transfer RNA) rRNA (ribosome RNA) snRNA (small nuclear RNA RNA nh nhõn) scRNA (small ... cytoplasmic RNA RNA nh t bo cht) hnRNA (heteroous nuclear RNA RNA khụng ng nht nhõn t bo) Chc nng ca cỏc loi RNA mRNA: Mang thụng tin di truyn di dng cỏc codon tRNA: Vn chuyn amino acid rRNA: Cu ... rRNA (ribosome RNA) loi rRNA: Tiu n v nh: rRNA 16S, kớch thc:1500 nucleotide Tiu n v ln: rRNA 23S, kớch thc: 2900 nucleotide rRNA 5S, kớch thc :120 nucleotide prokaryote (3) rRNA (ribosome RNA) ...

Ngày tải lên: 07/12/2015, 03:50

... transformation Number of Goi» Complete system omit A omit Adg DNA replace Adg DNA with A DNA replace Adg DNA with DNase-treated Adg DNA omit Adg DNA and A omit bacteria X 10't o 10 o o t A 10- dilution ... property of Adg DNA in the sense that neither A DNA nor DNase-treated Adg DNA can replace Adg DNA The results of both experiments are shown in Table The transforming activity of Adg DNA for Gal- ... anti-a anti-)' inactivates anti-T2 inactivatest") same as DNA more labile than DNA more labile than DNA same as DNA (1 '71 ) less than DNA (1-5) inactivates < ca 1% 50% I X 10· X 10· X 10' 2-5...

Ngày tải lên: 28/05/2016, 11:25

... falciparum) and chrPy1 °c 275 (P yoelii), A thaliana DRT111 and F26G16.10 proteins (gi 1169200 and 1522 075 7), D melanogaster CG 175 40 protein (gi 72 89585), C elegans F58B3 .7 protein (gi 75 04595) and H sapiens ... of the fulllength TgDRE cDNA The comparison of cDNA and genomic DNA nucleotide sequences allowed the determination of the exact position of the two introns which were 78 0- and 630-bp long (Fig ... corresponding to · 1 07 (1), · 108 (2) and · 108 (3) equivalent tachyzoites of T gondii 76 K strain Ó FEBS 2002 A novel T gondii DNA repair enzyme (Eur J Biochem 269) 33 97 Fig Resistance of bacteria...

Ngày tải lên: 08/03/2014, 22:20

... polymerase III Tổng hợp mồi (primer) RNA DNA polymerase I Nối dài mạch DNA kiểm soát tổng hợp Ligase Cắt mồi tổng hợp đoan DNA ngắn chỗ hở DNA topoisomerase I Hàn kín mạch DNA topoisomerase II Cắt hở ... tử DNA- RNA giai đoạn đầu, lõi enzime di chuyển theo chiều dọc phân tử DNA, tách sợi kép, ribonucleiotit tiếp tục gắn vào sau đoạn RNA mở đầu theo chiều 5’-3’ + Đoạn RNA tổng hợp tách khỏi DNA ... để tạo phức hợp Primosome .DNA Primose tổng hợp RNA mồi để giúp cho DNA- polymerase bắt đầu tổng hợp chuỗi DNA GIAI ĐOẠN KÉO DÀI Tại sợi mẫu cho tổng hợp chuỗi liên tục DNA polymerase với phân tử...

Ngày tải lên: 25/05/2016, 14:05

... quan DNA Chương 2: Chức chế truyền đạt thông tin di truyền DNA Nguyễn Thị Kim Nữ Sinh K22 Trang DNA – Trung tâm Sinh học phân tử NỘI DUNG CHƯƠNG : TỔNG QUAN VỀ DNA 1.1 SƠ LƯỢC VỀ DNA Acid deoxyribonucleic ... vận chuyển; (iii) Các gene mã hoá RNA ribosome; (iv) Đối với tế bào eukaryote, có gene mã hoá RNA chức đặc trưng khác như: iRNA, snRNA, snoRNA, scRNA, gRNA, RNA enzyme 61 spilicingvà telomerase ... so với DNA dạng B, DNA- Z dài gầy hơn, rãnh lớn bị dẹt phần bề mặt chuỗi xoắn; DNA dạng A ngắn to mập (Hình 7) Những vùng DNA có chứa purine pyrimidine xếp xen kẽ sợi tiếp nhận cấu hình DNA- Z,...

Ngày tải lên: 26/06/2015, 08:29

... diminished stability of ` PNA DNA vis-a-vis DNA duplex FEBS Journal 272 (2005) 4055–4 070 ª 2005 FEBS T Rathinavelan and N Yathindra Effect of A .C mismatch in PNA DNA and DNA duplexes Scheme Sequences ... Nielsen PE & Norden B (19 97) Kinetics for hybridization of peptide nucleic acids (PNA) with DNA and RNA studied with the BIAcore technique Biochemistry 36, 5 072 –5 077 15 Igloi GL (1998) Variability ... complexed with RNA Science 265, 77 7 78 0 52 Rasmussen H, Kastrup JS, Nielsen JN, Nielsen JM & Nielsen PE (19 97) Crystal structure of a peptide nucleic ˚ acid (PNA) duplex at 1 .7 A resolution Nat Struct...

Ngày tải lên: 07/03/2014, 21:20

... CHO-K1 caspase-3 cDNA (accession no AY 479 976 ) and Syrian hamster mRNA (accession no U 274 63) The caspase-3 genome sequence was determined from our cDNA (Genbank accession no FJ94 073 2; see Fig S1) ... the idea that AEBSF-sensitive, but FEBS Journal 277 (2010) 404–412 ª 2009 The Authors Journal compilation ª 2009 FEBS 4 07 Involvement of caspase-3 in DNA fragmentation kDa 150 - – + – – – – – ... including DNA fragmentation, supporting the above finding that caspase-3 is not essential for OA-induced DNA fragmentation in CHO-K1 cells FEBS Journal 277 (2010) 404–412 ª 2009 The Authors Journal...

Ngày tải lên: 22/03/2014, 21:20

... phage DNA concentration of 30 lM in 20 mM Tris/HCl, pH 7. 4 Various concentrations of L-ascorbic acid (10 lM to 150 lM) were added to the DNA solution CD spectra were recorded on a Jasco J -72 0 spectropolarimeter ... microscopic images of individual T4 DNA molecules in aqueous solution at pH 7. 4 in the absence and presence of ascorbic acid Depending on the concentration of ascorbic acid, DNA molecules exhibit intrachain ... mini-globules observed Ó FEBS 2003 Ascorbic acid induces conformational change in DNA (Eur J Biochem 270 ) 3103 on the fixed DNA may also exist on the segregated DNA in bulk solution Figure 3A,B shows...

Ngày tải lên: 23/03/2014, 21:20

... with the recovery of > 95% of DNA polymerase a and > 75 % of the other DNA polymerases in the extract Standard DNA polymerase activity and inhibition assays Total DNA polymerase activity was assayed ... dTTP, 50 mM 3-(N-morpholino)propanesulfonic acid buffer (pH 7. 5), 50 mM KCl, 10 mM MgCl2, mM EDTA and lg activated salmon testis DNA [7] Escherichia coli DNA polymerase I served as a positive control ... corresponded to % · 108 nuclei DNA polymerase a (110 UÆmL)1) was purified from plasmodia as described previously [11] DNase-I-activated salmon testis DNA for the standard DNA polymerase assay and for...

Ngày tải lên: 31/03/2014, 21:21

... • Dạng A -DNA (độ ẩm tương đối 75 %), dạng B DNA (độ ẩm – 92%) d B -DNA ẩ • DNA phân tử có dạng tuyến tính B DNA B -DNA (longitudinal view) B DNA B -DNA (lateral view) R.H helix A DNA A -DNA (longitudinal ... cấu trúc DNA RNA DNA RNA O O H3C NH NH N O H Uracil (U) N O H Thymine (T) HO CH2 H O Base H CH2 O Base H H H O HO H O OH H H 2'-deoxyribose ribose H Figure 2.5 The bases of DNA and RNA Figure ... A A and B DNA allomorphs d ll h DNA grooves 3.4 Một số đặc điểm vật lý DNA • Do vị trí vuông góc cấu tạo dạng vòng base mà phân tử DNA hấp thu 260 nm • Giả thuyết cấu trúc hai sợi DNA từ nhiễu...

Ngày tải lên: 19/06/2014, 12:14

... bacterial DNA sequences, which not contain introns, are typically the shortest genes 16 17 GENOMIC DNA The dideoxy DNA sequencing procedure was invented by Frederic Sanger and his colleagues in 1 977 ... strands of DNA All living organisms, from bacteria to human beings, contain DNA in each of their cells Each cell contains the entire genetic code for that organism 10 GENOMIC DNA DNA consists ... the DNA, and the sequenced DNA strands are sorted by size using capillary electrophoresis Finally, the DNA code is read by a computer, which displays the data for scientists to use 20 21 DNA...

Ngày tải lên: 29/06/2014, 13:20

... tương ứng RNA thông tin RNA vận chuyển mà quy định cho loại amino acid định (có khoảng 20 loại amino acid khác nhau) Một loại amino acid quy định số codon, nhiên codon mã hoá cho loại amino acid Có ... trình tự này, RNA thông tin tạo thông qua trình phiên mã Và từ RNA thông tin tế bào tổng hợp protein qua trình dịch mã thời điểm định đời Mỗi quan hệ trình tự gene với trình tự amino acid protein ... acid quy định số codon, nhiên codon mã hoá cho loại amino acid Có codon không mã hoá cho amino acid mà tín hiệu kết thúc vùng mã hoá (gọi làmã kết thúc Ở nhiều loài sinh vật, có phần nhỏ trình...

Ngày tải lên: 12/07/2014, 08:21

... NF-kappaB transcription factors J Biol Chem 19 97, 272 : 177 95- 178 01 Oyegunwa et al Journal of Inflammation 2010, 7: 59 http://www.journal-inflammation.com/content /7/ 1/59 23 DuBois RN, McLane MW, Ryder K, ... beta (b-Gal) (Clontech) and 300 ng of pcDNA6 (Invitrogen) were Oyegunwa et al Journal of Inflammation 2010, 7: 59 http://www.journal-inflammation.com/content /7/ 1/59 cotransfected into 293/TLR3, 293/TLR4-YFP/MD2 ... interacting with the DNA, preventing RelA from occupying its binding sites In support of this Oyegunwa et al Journal of Inflammation 2010, 7: 59 http://www.journal-inflammation.com/content /7/ 1/59 hypothesis,...

Ngày tải lên: 11/08/2014, 03:20

... Durability of Armored DNA Durability of Armored DNA Equal amounts of armored DNA and plasmid DNA (extracted from the armored DNA) were incubated with DNase I (0.1 units/μl) at 37 C for 60 After digestion, ... EcoRI DNA fragments of Escherichia coli and phage P1 Gene 1 977 , 1:255-280 Hohn B, Murray K: Packaging recombinant DNA molecules into bacteriophage particles in vitro Proc Natl Acad Sci USA 1 977 , 74 :3259-3263 ... real-time PCR The armored DNA was completely resistant to DNase I digestion, whereas plasmid DNA was degraded completely Armored DNA and plasmid DNA indicate samples without DNase I digestion methods,...

Ngày tải lên: 12/08/2014, 04:21

... Gamow G: Possible relation between deoxyribonucleic acid and protein structures Nature 1954, 173 :318 Gamow G: Possible mathematical relation between deoxyribonucleic acid and proteins Biol Med 1954, ... (one for each amino acid) where the amino acid residues aligned and became ligated The correct translation turned out to be mRNA-mediated and stereochemical fitting between DNA and protein residues ... structure, dsDNA However, partial or suboptimal WC complementarity in and between strands provides a large number of DNA/ RNA structure variations The structural variation of a given RNA might be...

Ngày tải lên: 13/08/2014, 23:20

... Biochemistry 1991, 30, 11 377 70 Morgan, A R.; Lee, J S.; Pulleyblank, D E.; Murray, N L; Evans, D H Nucleic Acids Res 1 979 , 7, 71 Renneberg D.; Dervan, P B J Am Chem Soc 2003, 125, 570 7 72 Harshman, K D.; ... Invest New Drugs 1991, 9, 95 77 Spink, N.; Brown, D G.; Skelly, J V.; Neidle, S Nucleic Acid Res 1994, 22, 16 07 78 Searle, M S.; Embrey, K J Nucleic Acid Res 1990, 18, 375 3 79 Teng, M K.; Usman, N.; ... Protocols 76 3.5.1 Materials 76 3.5.2 HT-FID Assay 76 3.6 List of References 77 APPENDICES Appendix A 1H NMR Spectra 78 Appendix B Mass Spectra...

Ngày tải lên: 24/08/2014, 11:27

... 10 0-8 9-6 11-4 8 -7 Y90823 TCAB 44-9 54-1 0-8 8-8 PCA 48-2 47. 7 b 47. 31 48-1 e 47 -6C Y90621 Y9062I" Y91115 46-9 56-4 PCA, perchloric acid TCA, chilled 0-5m trichloroacetic acid TCAB, buffered ... Biophysics, 1952, 40, 175 Oura, E & Suomalaincn, H., Journal of the Institute of Brewing, 19 67, 73 , 370 Piperakis, S M & Piperaki, F M., Folia Biochimica et Biologica Graeca, 1 977 , 14, 32 Pomcranz, ... Food and Agricullure, 1 977 , 28, 579 19 Trevelyan, W E., Journal of the Science of Food and Agricul ture, 1 978 , 29, 903 20 Tubb, R S., Journal of the Institute of Brewing, 1 979 ,85,286 21 Zimmermann,...

Ngày tải lên: 14/07/2015, 09:44

... NUCLEIC ACIDS 74 2.1 Introduction 74 2.2 Materials and Methods .77 2.2.1 Reagents 77 2.2.2 Preparation of conventional and CNT-based labels 79 2.2.3 ... phosphate group In deoxyribonucleic acid (DNA) , the bases are the purine adenine (A) and guanine (G) as well as the pyrimidines cytosine (C) and thymine (T) In deoxyribonucleic acid (RNA) , T is replaced ... Targets hybridization 1 57 5.2.6.3 Hybridization with CNT-based labels .1 57 5.2 .7 Samples assay 1 57 5.2 .7. 1 HRP enzymatic reaction 1 57 5.2 .7. 2 Electrochemical detection...

Ngày tải lên: 12/09/2015, 09:28

... bicyclic diketones 1-14 and 1- 17 was reported by two industrial research groups in 1 970 s ,74 ,75 it was reported that ent-1-14 could be obtained with 83% ee by employing β-amino acid 1-38 as catalyst.150 ... transition state model 106 Figure 5-1 Acid- promoted DNA cleavage by varacin B in sodium phosphate buffer solutions at various pH values 203 Figure 5-2 Acid- promoted DNA cleavage by various concentrations ... DMA N,N-dimethylacetamide DMF N,N-dimethylformamide DMSO dimethylsulfoxide d doublet DNA deoxyribonucleic acid dr diastereomeric ratio - xvi - PhD Thesis Jiang Zhaoqin EA ethyl acetate ee enantiomeric...

Ngày tải lên: 14/09/2015, 08:39