deoxyribonucleic acid and ribonucleic acid

... active molecules carry dg, c, and mi Assuming that the entire dg region, as mapped by Arber (1958), is present, and that the segments between dg and c, and between c and mi are also present, this ... this phage suspension and freshly distilled phenol saturated with water at °C were mixed and agitated by hand for m in The two phaaes were separated by centrifugation and the aqueous phase recovered ... CsCI-3) and of Mg DNA at pH The phage were suspended in 0·01 M-MgSO and 0·01 M-potassiurn phosphate buffer and no correction was made for scattered light The DNA was dissolved in 0·15 M-NaCI and...

Ngày tải lên: 28/05/2016, 11:25

... The ligand-dependent interaction between AR NTD and AR LBD, N/C interaction, was studied in yeast and mammalian in vivo protein interaction systems, and in Ó FEBS 2002 Interaction between androgen ... 15 and 37, the putative a-helix is flanked by proline residues Within the helix, two candidate FxxFF protein interaction motifs (F is any hydrophobic amino acid residue and x is any amino acid ... amino acids 23, 26 and 27 completely abolished interaction with GalDBD-AR.LBD and alanines at positions 24 and 25 increased the interaction capacity All alanine substitutions of amino acids flanking...

Ngày tải lên: 17/03/2014, 10:20

... Trebouxia erici and Trebouxia impressa (cultures from the Botanical Institute, St Petersburg, Russia) not contain the 18 : and 18 : fatty acids, and produce only two fatty acids: 16 : (30% and 51%, ... respectively) and 18 : (69% and 42%, respectively) It is probably an artefact of the GC analysis Mycobionts produce saturated fatty acids such as tetradecanoic acid (5–9%), hexadecanoic acid (34–37%) and ... library (7th edition) Results and discussion The complex hydrocarbons and fatty acids produced by the cultured photobiont green alga Trebouxia sp and the mycobiont, and also by X parietina, were...

Ngày tải lên: 23/03/2014, 17:21

... Waldbodenbericht 1996, Band Bundesministerium fỹr Ernọhrung, Landwirtschaft und Forsten, 1999 Eect of acidication and liming on tree nutrition APPENDIX Mean K, Ca, Mg, Mn, Fe and Al concentrations ... input between May and November by about 50% Tap water was deionised and adjusted to rain water conductivity (1020 àS cm1 ) through the addition of Ca(NO3 )2 and MgSO4 For acid irrigation, a ... Eect of acidication and liming on tree nutrition 863 Figure Mean N concentrations of current (C), current + one year (C+1), and current + two year needles (C+2) at the A1 (control), B1 (acid irrigation),...

Ngày tải lên: 07/08/2014, 16:20

... between germination inhibitors (e.g abscisic acid, ABA) and growth promoters (e.g gibberellin acid, GA) is a main cause of seed dormancy breaking and germination (hormone balance theory of dormancy) ... nine spots were regulated by both ABA and GA Of this group, six spots were down-regulated and three up-regulated by ABA and four spots were down-regulated and five up-regulated by GA Eight spots ... analysis and is being used more and more in studies on seed development, dormancy, dormancy breaking and germination [18,20,21] Here, a proteomic analysis of seed dormancy breaking and germination...

Ngày tải lên: 12/08/2014, 03:20

... to oxalic acid, L-threonic acid and L-tartaric acid [reviewed in [47,48]], Figure In geraneaceous plants, Wagner and Loewus [49] demonstrated that cleavage of Asc between carbon atoms and results ... ascorbate (tAsc) and the ascorbate catabolites tartaric (TA) and oxalic acids (OA) Figure Accumulation of total ascorbate (tAsc) and the ascorbate catabolites tartaric (TA) and oxalic acids (OA) All ... designed and conducted all research experiments, analysed the data, and drafted/constructed the manuscript CMF supervised all research CMF and KLS contributed to the research ideas and design, and...

Ngày tải lên: 12/08/2014, 03:21

... 20 m (Davies et al 1997, Foale 2006) and bleaching was also observed in areas near Motupore and Madang and the Lihir Islands and Lak region near New Ireland (Srinivasan 2000) In 1999-2000 increasing ... organisms and the effect of increased CO2 on seawater acidification and the calcification/dissolution processes a-b: red and green calcareous benthic algae, c: foraminifera, d-e: shallow and deep-water ... reefs north of the Solomon and Vanuatu Island groups, and by 2040-49 this area expands to include waters and reefs north of PNG By 2060-69 both thermal stress and marginal to submarginal saturation...

Ngày tải lên: 08/06/2016, 11:26

... Cyanuric Acid Isotopically labeled Cyanuric Acid (Cambridge Isotope Labs #CNLM4661-1.2) 13C315N3 labeled Cyanuric Acid Alternate Source for Isotopically Labeled Cyanuric Acid: Isotec; 13C3 Cyanuric Acid ... Results and Discussions A fast, sensitive, and specific method was developed for the determination of melamine and cyanuric acid in foods Even though the original method was developed and validated ... containing incurred residues of melamine and cyanuric acid and FDA Forensic Chemistry Center for catfish containing incurred residues melamine and cyanuric acid The authors also wish to thank Gonçalo...

Ngày tải lên: 06/10/2016, 09:29

... flap was elevated and the granulation tissue was removed showing the true extension and depth of the infra-bone defect Debridement and root preparation were carried out with hand and ultrasonic ... were made at the first examination and saved for follow-up control examinations Radiographs were performed immediately before treatment and at 10 days, and 6, 9, and 24 months after treatment 66 ... of plaque was registred mesially, buccally, distally, and lingually Data were obtained at baseline before treatment and at 10 days, and 6,9, and 24 months after treatment Surgical Technique After...

Ngày tải lên: 03/11/2012, 11:35

... products from DCAA and TCAA by o hydrothermal reaction at 250 C and MPa Journal of Water and Environment Technology, Vol.1, No.2, 2003 acid, % of glycolic acid and % of formic acid were detected ... organic acid, chlorine ion and DOC, all samples were filtered to separate solid materials, using micro-syringes and filters with pore size of 0.45 µm RESULTS AND DISCUSSION Glycolic acid Formic acid ... malic acid decreased, and was converted into more simple organic acids as reaction time increased Glycolic acid increased up to 28 % at 27 min, and then decreased gradually However, formic acid...

Ngày tải lên: 05/09/2013, 08:40

... fatty acids, and the ratio of each fatty acid RESULTS AND DISCUSSION Characteristics of carbon stable isotope in Z japonica The mean δ13C of Z japonica was -12.4 ± 1.6‰ in the leaves and stems, and ... the roots and rhizomes Meanwhile, Z marina in the leaves and stems has -9.7 ± 1.0‰, and in the roots and rhizomes has -9.6 ± 1.0‰ Fig shows that the leaves and stems as well as the roots and rhizomes ... We found about 30 kinds of fatty acids in the leaves and stems and about 36 kinds of fatty acids in the roots and rhizomes of Z japonica We focused on the fatty acid content of Z japonica , because...

Ngày tải lên: 05/09/2013, 10:17

... hydroperoxides increases the risk of polymerization and acidification and of the appearance of insoluble sediments and gums, which can lead to filter plugging and deposits in the fuel systems Esterified ... of Energy and Environment (IJEE), Volume 1, Issue 3, 2010, pp.411-430 427 properties and fatty acid composition on CO and HC emissions The correlation analysis reveals that the CO and HC emissions ... and the product was allowed to settle in two layers The upper layer consisted of ester and alcohol and was separated from the bottom layer (glycerin) The upper layer was distilled to remove and...

Ngày tải lên: 05/09/2013, 15:28

... 38 and 22.1 ( 15.2 g -carotene/g FW in extracted samples of aril and mesocarp, respectively, and 16 and g/g of zeaxanthin and 35 and g/g of -cryptoxanthin in saponified samples of mesocarp and ... relative standard deviation, % vaccenic (in two fruit), R-linolenic, and eicosa-11-eneoic acids were also detected The fatty acid composition of the aril and seed are interesting, and they reflect ... Internal standard (nonadecanoic acid methyl ester) was added, and the extraction took place at 55 °C for 30 with shaking every 10 Extracts were filtered and dried over sodium sulfate, and the solvent...

Ngày tải lên: 09/02/2014, 00:08

... essential oil and shikimic acid from star anise fruits With this method, obtaining yield of essential oil and shikimic acid respectively are 9.5% and 5.6% (essential oil content and shikimic acid content ... Sciences and Technology 26 (2010) 110-113 for obtaining simultaneously essential oil and shikimic acid from star anise fruits The yield of essential oil and shikimic acid were 9.5% and 5.6% (r.m), ... obtained by methods N0 1, N0 and N0 were shown at table and table Table Obtaining simultaneously essential oil and shikimic acid from star anise fruits by methods N0 1, N0 and N0 Method N0 N0 N0 Star...

Ngày tải lên: 12/02/2014, 17:20

... acid as a major plasma metabolite of 9-cis-retinoic acid and limited transfer of 9-cis-retinoic acid and 9,13-dicisretinoic acid to the mouse and rat embryos Drug Metab Dispos 22, 928–936 Shirley ... (A, lanes and 5; B, lanes and 5) or 10 lM S-4o9cDH-RA (A, lanes and 6; B, lanes and 6), followed by incubation with trypsin or buffer only as indicated (for details, see Materials and methods) ... RARa and RXRb (A), RARa and RXRb (B), RXRa (C) or RXRb (D) Cells were treated with S-4o9cDH-RA at the indicated concentrations at-RA (100 nM) was used as a positive control in (A) and (B), and...

Ngày tải lên: 18/02/2014, 08:20

... barrel between the b3-strand and the a3-helix; (b) the catalytic machinery consists of the b4-strand aspartate (nucleophile), b5-strand glutamate (proton donor) and b7-strand aspartate (transition-state ... especially in higher animals (mammals and also in frogs and fishes), an aspartate (aspartic acid 248 in human 4F2hc; aspartic acid 380 in Fig as both the N-terminal and transmembrane segments are involved) ... exception of those from birds, lizards and frogs (lacking both essential aspartates at the b4- and b7-strands) and also from some fishes (lacking the b4-strand aspartate) This may mean that the...

Ngày tải lên: 18/02/2014, 13:20

... structure and function [4] In this minireview, we focus on how diarrheic shellfish toxins (OA, dinophysistoxins and analogs) affect Ser ⁄ Thr protein phosphatases and their effects on cell adhesion and ... present in mammalian cells, PP1 and PP2A, with IC50 values of 0.2 and 20 nm for PP2Ac and PP1c inhibition, respectively [5–7] Being hydrophobic, OA can enter cells, and it has been shown that it ... compilation ª 2008 FEBS 6061 DSPs and the cytoskeleton C Vale and L M Botana Diarrheic shellfish toxins and cell adhesion The adherence of cells to each other and to the elaborate mesh that comprises...

Ngày tải lên: 18/02/2014, 14:20

... 20–25 27% Buffer B, 25–58 27–42.5% Buffer B, and 58–63 42.5–100% Buffer B Buffer A is 0.1% trifluoroacetic acid and Buffer B is 0.1% trifluoroacetic acid in 70% (v ⁄ v) acetonitrile The flow rate ... kinetics of the Trp14 and Thr15 amide protons The two observed small 3JHN-Ha coupling constants for residues Asn11 and Ser12, and the dNN(i,i + 2), daN(i,i + 2) [Phe13(CaH)–Thr15(NH)] and daN(i,i + 3) ... small 3JHN-Ha coupling constants for residues Asn11 and Ser12 and the dNN(i,i + 2), daN(i,i + 2) [Ser12(CaH)–Trp14(NH) and Asn11(CaH)–Phe13(NH)] and daN(i,i + 3) [Asn11 (CaH)–Trp14(NH)] NOEs in...

Ngày tải lên: 18/02/2014, 17:20

... contamination from xylem, and vice versa Glx (glutamine and glutamate: ve carbon amide and amino acid) and Asx (asparagine and aspartate: four carbon amide and amino acid) were found to be the ... Amino acid composition in leaves, and amino acid percentage ratio in the phloem exudates and leaves and xylem bleeding sap and leaves in the light and dark The amino acid composition in leaves is ... (B) and amino acids (CI) in maize leaves collected every h during a 16 h light h dark cycle Nitrate and ammonium contents represent the mean from ve independent plants standard error Amino acid...

Ngày tải lên: 18/02/2014, 18:20

... cation ligands are main-chain oxygen atoms, and three are the side-chain oxygen atoms of N296, E342 and S387 The glutamic acid and asparagine residues are conserved among known KAS I and KAS II ... mutants K328H and K328R are more efficient (247% and 227%), the acidic mutant K328E is less efficient (31%), and the bulky mutants K328F and K328I are similar to the wild-type (91% and 117%) Increasing ... two basic mutants, K328H and K328R, and the bulky mutant, K328F, evinced some activity (Fig 7A, lanes 7–12, and Fig 7B, lanes 2–4) By comparison, the acidic mutant K328E and to a lesser extent...

Ngày tải lên: 19/02/2014, 08:20