dendritic cells in asthma and copd

... matrix proteins may play a role in the remodelling of airways and blood vessels in COPD In COPD patients, as compared to non -COPD patients an increased staining for fibronectin in the neointima, ... changes in the bronchial wall in both asthma and COPD, there are distinct differences in detail between these situations Angiogenesis seems to be a typical finding in asthma, while, in COPD, little ... remodelling and respective functional effects in asthma and COPD Structural changes Functional effects Asthma COPD Vascular remodelling with inner airway wall thickening Decreased baseline airway...

Ngày tải lên: 12/08/2014, 11:22

... sources included in the model were: road traffic, shipping, aviation, railroad, industries and larger energy and heat producers, small scale heating, working machines, working vehicles, and working ... especially in relation to the prevalence of COPD/ chronic bronchitis Results discussion Although asthma and COPD have many risk factors in common and often coexist in clinical settings, and there ... restricting the groups to those with asthma but not COPD, and COPD but not asthma, to exclude confounding by comorbidity This process was also followed for symptoms Relative risk was estimated using...

Ngày tải lên: 06/03/2014, 19:20

... * HRQoL, COPD, and asthma In the current analyses, we chose to join self-reported asthma and spirometrically defined COPD in one group Firstly, aging is an established risk factor for COPD [17,30,31] ... shortness of breath in the last 12 months Woken by attack of coughing in the last 12 months Usually cough first thing in the morning in winter? Usually cough in day or night during winter? Voll-Aanerud ... Questionnaire information Information on respiratory symptoms was obtained in ECRHS II by questions 1-11 listed in appendix Asthma was defined as ever having had asthma, question 12 in appendix...

Ngày tải lên: 20/06/2014, 16:20

... from the remaining samples after routine blood examination was performed according to DEM procedures Routine tests undertaken include full blood examination, electrolyte examination and ECG measurement ... between QTc interval and albuterol levels Recorded QTc interval and (R)-, (S)- and total albuterol levels and are shown in (a), (b) and (c) respectively (R)-, (S)- and total albuterol levels in subjects ... function and QTc interval in patients with acute severe asthma and COPD in the emergency department International Journal of Emergency Medicine 2011 4:30 Submit your manuscript to a journal and benefit...

Ngày tải lên: 21/06/2014, 03:20

... these cells from the circulation into the site of inflammation The treatment in our in vitro model with cocktails containing Th1 inducing or inflammatory cytokines and TLR agonists mimics the in ... potent pro-inflammatory cytokines and chemokines The hall-mark cytokines involved in initiation of the adaptive Th1 immune responses are IL-12p70 whereas IL-6, TGFb and IL-23 are involved in initiation ... CCL18, CCL22 and APRIL, Page of 12 involved in both Th1 and Th2 type responses [8,9] The unique and very complex signalling network in DCs involving PRRs and secretion of early triggers of inflammation,...

Ngày tải lên: 11/08/2014, 06:22

... dendritic cells with interleukin and CD40-ligand J Exp Med 1996, 185:1101-1111 Grouard G, Durand I, Filgueira L, Banchereau J, Liu Y-J: Dendritic cells capable of stimulating T cells in germinal ... and Vaccines 2004, Origin and developmental processes of dendritic cells DCs originate from hematopoietic stem cells in the bone marrow Recently, there have been great insights into the origins ... antigen-specific B cells [29] T- and B-cell interaction results in the clonal expansion of B cells, which takes place in the plasma foci of the T cell rich area [30] and in the germinal centers [31] T- and...

Ngày tải lên: 11/08/2014, 10:23

... marrow cells; FLDC, dendritic cells induced by FL; FL, FMS-like tyrosine kinase ligand; GMCSF, granulocyte macrophage-colony-stimulating factor; IL-4, interleukin 4; mDCs, myeloid dendritic cells ... FLDCs, dendritic cells induced by FL; mDCs, myeloid dendritic cells induced by GM-CSF + IL-4; FL, FMS-like tyrosine kinase ligand; GM-CSF, granulocyte macrophage-colony-stimulating factor; IL-4, interleukin ... FLDCs, dendritic cells induced by FL; mDCs, myeloid dendritic cells induced by GM-CSF + IL-4; FL, FMS-like tyrosine kinase ligand; GM-CSF, granulocyte macrophage-colony-stimulating factor; IL-4, interleukin...

Ngày tải lên: 12/08/2014, 12:20

... airway T cells are highly activated in COPD as indicated by increased expression of CD69 and HLA-DR [6] In addition, CD4+ cells express high levels of CD25 in COPD and smokers, suggesting the ... CD3+, CD4+ and CD25+ cells, the cells were stained with same antibodies as in the extracellular staining To obtain FoxP3+ cells, phycoerytrin (PE) conjugated anti-human FoxP3 was used in the same ... airway regulatory T cells in smokers and individuals with COPD, using flow cytometric analysis of CD127 and FoxP3 and their relation to CD25 expression Materials and methods The COPD patients did...

Ngày tải lên: 12/08/2014, 13:22

... useful in monitoring asthma and COPD patients during infectious episodes, but this clearly requires further investigation Decreased pH values in EBC in patients with inflammatory diseases like asthma ... (HC), asthma, COPD and cold Comparison of pH in EBC obtained by either RTube or ECoComparison of pH in EBC obtained by either RTube or ECoScreen in healthy controls (HC), asthma, COPD and cold ... manuscript SD helped in acquiring patient data PH, PS, CV, RB, RS and KR were involved in drafting and revising the manuscript SG carried out the logistics and appointed all included study patients...

Ngày tải lên: 12/08/2014, 14:20

... We combined polythymidine and F508del genotypes [1], and examined associations between 11 different genotype combinations, and annual FEV1 decline and risk of asthma or COPD We also examined whether ... decline of 14 ml in 9T/9T, 3.8 ml in 7T/9T, 61 ml in 6T/ 7T, 23 ml in 5T/9T, ml in 5T/7T, 31 ml in 9T/9T F508del, ml in 7T/9T F508del, 72 ml in 7T/7T F508del, and 72 ml in 5T/9T F508del individuals ... Intron-8 polythymidine tract and ∆F508 genotype Figure thymidine tract spirometry genotype Odds ratios for and F508deldefined COPD by intron-8 polyOdds ratios for spirometry defined COPD by intron8...

Ngày tải lên: 12/08/2014, 18:22

... Adaptive immune system in asthma 46 1.9.1 B cells in asthma 47 1.9.2 Th2 cells in asthma 47 1.9.3 Th1 cells in asthma 48 1.9.4 Th17 cells in asthma ... contributing airway inflammation and remodelling (Bousquet et al., 2000) 1.5 Innate immune cytokines and asthma There is increasing evidence suggesting that innate immune mechanisms, involving an ... family of innate effector cells involved in asthma are natural killer T cells (NKT cells) with a unique feature of both classical T cells and NK cells Particularly, invariant NKT cells (iNKT cells) ...

Ngày tải lên: 10/09/2015, 09:04

... cells/ well Cells were cultured in RPMI 1640 (Invitrogen) supplemented with 10% FCS (Hyclone), 1% Lglutamine and 1% Penicillin/Streptomycin (Gibco) in the presence of recombinant human Fms-like tyrosine ... nature of the principal type interferon-producing cells in human blood Science 1999, 284:1835-1837 Liu YJ: IPC: professional type interferon-producing cells and plasmacytoid dendritic cell precursors ... these cells are BDCA2 and BDCA4 (type II C lectin)-positive and CD11c- and CD1a-negative Trypan blue exclusion and cell counting of LV and rAAV transduced GEN2.2 at the end of the culture period indicated...

Ngày tải lên: 18/06/2014, 15:20

... and HepG2/ADM cells The drug-resistant HepG2/ADM cells were cultured in the cell culture medium containing μg/ mL adriamycin (Sigma) Both cell lines were maintained in RPMI-1640 medium containing ... mice using a 1.0 mL syringe Intravenous injection of reagents and tumor growth inhibition study The nude mice inoculated with HepG2/ADM cells were divided into four groups with seven mice in each ... DNR-mediated apoptosis in HepG2r/ADM cells, we investigated apoptosis-related protein expression in the cells (Figure 6C) DNR or Cdte QDs cannot induce apoptosis strongly in HepG2r/ADM cells due to multidrug...

Ngày tải lên: 21/06/2014, 03:20

... centrifugation Cells were resuspended in buffer containing 0.1% bovine serum albumin and 0.1% NaN3 at 106 cells/ ml Nonspecific antibody binding at the Fc receptor was blocked by treating 106 cells with ... antigen-experienced T cells, are mainly CD28– and can also express CCR5 [10,23,24] The fraction of CD28– T cells is also expanded in WG and is correlated with organ involvement [25–28] These findings support ... CCR5+ and CCR3+ T cells owing to enhanced recruitment into inflammatory sites Downregulation of the cell-surface expression of the inducible inflammatory chemokine receptors CCR5 and CCR3 during...

Ngày tải lên: 09/08/2014, 01:21

... assay and GEM assay experiments, assisted in data analysis and interpretation and in writing and revising the manuscript SP and JG carried out the Western blot experiments CH and SA assisted in ... proangiogenic and pro-inflammatory factors, VEGF, COX-2, IL-6 and IL-8, in cultured human colon cancer cells; (c) DCPIP and DCPIP NPs are similarly effective in inhibiting VEGF-induced angiogenesis in ovo; ... the design planning of all experiments PP participated in the design of XTT, CAM and Western experiments, assisted in data analysis and interpretation and in writing and revising the manuscript...

Ngày tải lên: 11/08/2014, 00:22

... and Vaccines 2004, 2:8 DCI E-64 100 80 40 20 Cathepsin D/E Cathepsin C Cathepsin A Chymotrypsin BLT Esterase Cathepsin L Cathepsin J Cathepsin H 0 Cathepsin A Cathepsin L Cathepsin J Cathepsin ... expressed as nmol/min/mg soluble protein, in which protein concentrations were measured by the bicinchoninic acid method using bovine serum albumin as a standard [32] Ag presentation and T cell stimulation ... Cathepsin H Cathepsin D/E Cathepsin C Chymotrypsin BLT Esterase Cathepsin A Chymotrypsin BLT Esterase Cathepsin L Cathepsin J Cathepsin H Cathepsin D/E Cathepsin C 20 20 40 60 60 40 % Inhibition 80...

Ngày tải lên: 11/08/2014, 10:23

... http://arthritis-research.com/content/12/4/R137 metric detection of annexin V and propidium iodide (PI) staining (double positive cells) Interaction of dendritic cells with apoptotic cells pDCs were incubated with apoptotic PMNs for ... expression including MHC-II CD86 CD83 and CCR7 were found in pDCs from both SLE and healthy [18] T cells were defined as CD3+ cells and its purification is about 99% (data not shown) Changes in surface ... of 11 Figure Changes in mRNA expression and protein levels of cytokines in HD and SLE pDCs + apoPMNs (a) Changes in IFNα, IL-6, IL-10 and IL18 mRNA expression in HD (n = 7) and SLE (n = 7) pDCs...

Ngày tải lên: 12/08/2014, 14:22

... polymorphisms in candidate genes for asthma/ atopy have been identified Linkage and candidate gene studies in asthma and atopy Linkage studies Results from linkage studies (genome-wide searches and candidate ... of inconsistent findings, it is intriguing that chromosomal regions linked to asthma have also shown evidence for linkage to other inflammatory and autoimmune diseases (such as psoriasis, inflammatory ... other genes involved in the allergic inflammation (e.g IL-4Rα [21], β2AR [24]) We can therefore speculate that inconsistent findings in the genetic studies of asthma and atopy summarized in this...

Ngày tải lên: 12/08/2014, 18:20

... polypyrimidine tract binding protein, appears to account for the underlying mechanism in resting CD4+ T cells Transient expression of this host protein induces productive HIV-1 replication in resting ... [reviewed in [127]] In resting CD4+ T cells from HIV-1-infected individuals, HIV-1 multiply spliced RNA transcripts are retained in the nucleus and cannot be translated into functional proteins [128] ... an inducible provirus was rescued from the cells after addition of the inducing doxycycline drug, indicating that HIV-1 is capable of establishing latency in the majority of actively dividing cells...

Ngày tải lên: 12/08/2014, 23:21

... corresponding cDNA, was observed in immature dendritic cells as well as in rat brain lysates (Fig 3C) Fig Western immunoblotting of protein homogenates of human immature dendritic cells, rat brain and ... examined DISCUSSION The results presented here indicate for the first time that human dendritic cells contain anandamide, 2-AG and PalEtn The amounts of anandamide and 2-AG in immature dendritic cells ... Conversely, T cells, after interacting with dendritic cells, might produce anandamide and 2-AG, which then might feed back on CB1 Ó FEBS 2002 The endocannabinoid system in human dendritic cells (Eur...

Ngày tải lên: 22/02/2014, 07:20