definition classification and characterization of anemia in cancer

... immunostaining) Overexpression of Trip10 in IMR-32 cells caused Trip10 and huntingtin to colocalize and form perinuclear foci In contrast, while overexpression of Trip10 in CP70 cells also increased ... tumorigenesis and metastasis in mice inoculated with Trip10-overexpressing IMR-32 cells (Figure 4A) On the other hand, huntingtin increases cell death by promoting apoptosis Thus, high levels of huntingtin ... profound effect in the cell type-specific functions of Trip10 Discussion Trip10 was initially identified as a Cdc42-interacting protein involved in GLUT4-mediated glucose uptake in adipocytes and...

Ngày tải lên: 10/08/2014, 05:21

... Utilization of peptides and proteins by ectomycorrhizal fungi New Phytol 103, 481-493 Abuzinadah R.,A., Finlay R.D & Read D.J (1986) The role of proteins in the nitrogen nutrition of ectomycorrhizal ... the ability of the fungi to produce such enzymes and their distribution in the soil layers References Abuzinadah R.A & Read D (1986) The role of proteins in the nitrogen nutrition of ectomycorrhizal ... cultures grown in the presence of proteins from the litter, but protease excretion was reduced Protease activity was also induced in beech-Lactarius ectomycorrhizas incubated in the presence of litter...

Ngày tải lên: 09/08/2014, 04:20

... respiratory infections to CF patients In PCD, neutrophilic lung inflammation, incidence of lung infection, offending organisms, development of bronchiectasis and longitudinal declines in lung function ... dehydrated in solutions of ethanol of increasing strength from 50%, 70%, 95% and 100% for 10 minutes in each solution The sample was finally dehydrated in propylene oxide for 15 minutes Finally, ... Phenotype of MPs present in sputa of acute and stable CF and PCD patients In acute and stable CF patients, the number of MPs staining positive for FITC-conjugated antibodies directed against CD11a...

Ngày tải lên: 12/08/2014, 11:22

... profile of chromosome for breast tumor (data set 1) with SNPs >0.97 in mBAF removed CBS segmentation profile is superimposed in red Horizontal dashed line indicates position of 0.9 in mBAF and ... (Courtesy of Illumina Inc.) Reference data set consists of 120 HapMap samples hybridized on Illumina HumanHap300 version Genotyping BeadChips (Courtesy of Illumina Inc.) Reference data set consists of ... Illumina HumanCNV370 Genotyping BeadChips (Courtesy of Illumina Inc.) Reference data set consists of 120 HapMap samples hybridized on Illumina HumanHap550 Genotyping BeadChips (Courtesy of Illumina...

Ngày tải lên: 14/08/2014, 20:22

... family size and timing • Contact details of those providing support and information, including local and national support groups • The message that, to help provide support and understanding of the ... hereditary breast cancer • Information about genetic testing, both predictive testing and mutation finding, including details of what the tests mean and how informative they are likely to be, and the likely ... number of false positive results and the resulting increase in cost of those incorrectly brought back for further investigation In making any surveillance decision, the benefits and risks of each intervention...

Ngày tải lên: 15/03/2014, 00:20

... sole clinical mix journal in the set, maintains links to both the clinical domain and the molecular biology domain This journal plays a key role linking diverse research interests while 267 of the ... descriptions of widely used methods and techniques One interesting set are the guidelines and criteria of various sorts seen in both the cancer plot (Boland 1998, Mountain 1997, Therasse 2000, Sobin 2002) ... advances in understanding of cancer genetics and increasing use of surface antigens to characterize cell types, yielded a hybrid of applied and basic research with clear relevance for clinical...

Ngày tải lên: 18/06/2014, 19:20

... sole clinical mix journal in the set, maintains links to both the clinical domain and the molecular biology domain This journal plays a key role linking diverse research interests while 267 of the ... descriptions of widely used methods and techniques One interesting set are the guidelines and criteria of various sorts seen in both the cancer plot (Boland 1998, Mountain 1997, Therasse 2000, Sobin 2002) ... advances in understanding of cancer genetics and increasing use of surface antigens to characterize cell types, yielded a hybrid of applied and basic research with clear relevance for clinical...

Ngày tải lên: 20/06/2014, 03:20

... all-alpha domain, STAT protein DNA-binding domain and SH2 domain of stat6, and the zinc-finger domain, leucine-zipper domain and fork-head domain of foxp3 In addition, for stat6 and foxp3, there ... implicated in other protein–protein interactions, a STAT protein DNA-binding domain and an SH2 domain, which binds phosphorylated tyrosine residues in the context of a longer peptide motif within a ... binding and nuclear localization), which is shared by all FOX proteins, a leucine zipper domain and a C2H2 zinc finger domain, both of which are thought to help mediate DNA binding and may be involved...

Ngày tải lên: 16/02/2014, 09:20

... N-acetylornithine deacetylase; OCT, ornithine carbamoyltransferase; ASS, argininosuccinate synthase and ASL, argininosuccinate lyase by arginine [16,17] As a result, the concentration of citrulline and ... mitochondria [15,30,31], and ornithine is exported to the cytosol to be converted into citrulline and arginine [31,32] In plants, information on the localization of citrulline and arginine biosynthetic ... owing to its high thermostability and insensitivity to the inhibition induced by citrulline and arginine However, many different enzymes are involved in the metabolism of citrulline (Fig 1), and...

Ngày tải lên: 20/02/2014, 03:20

... members of this family including UspA itself (Fig 2), and one larger protein consisting of two UspA domains in tandem [17] The small members of this family are proteins of 142–144 amino-acids ... Department of the Weizmann Institute by a single injection of 150 lg of the purified protein, followed by two booster shots of the same amount of protein at 2-week intervals Serum was collected and used ... growth and expression of UP12 Absorption (upper panel) and relative amount of UP12 (lower panel) in samples of cells grown in minimal M9 medium containing limited concentration of glucose (m) or in...

Ngày tải lên: 22/02/2014, 07:20

... (S)-cheilanthifoline, not (S)-nandinine [7,30,31] If we assume that (S)-nandinine is produced in E californica, there may be no need for a catalyst from (S)-nandinine to (S)-stylopine However, nandinine could ... v ⁄ v ⁄ v) and the flow rate (0.5 mLÆmin)1) To determine the kinetic parameters of CYP719A2, the amount of nandinine produced was estimated by using a calibration curve of (S)-scoulerine (pmol ... determine the kinetic parameters of CYP719A3, the amount of nandinine produced was estimated by using the calibration curve of (S)-scoulerine (pmol versus peak area) and the absorption ratio of...

Ngày tải lên: 07/03/2014, 10:20

... DNA binding domain Ligand binding domain Fig Sequence alignment (A) Alignment of DNA binding domain (C domain) and its C-terminal extension (B) Alignment of ligand binding domain (E domain) (after ... SmNR1(Ile247-Ser372) and SmRXR1(Glu251-Asn376) in vitro DNA binding of a protein containing 20 amino acids at the 5¢ end of the DBD, the DBD and the 40 amino acids at the 3¢ end of the DBD of SmNR1 (Ile247-Ser372) ... extension of SmNR1 and SmRXR1 in binding to DNA elements, in vitro synthesized SmNR1 (Ile247 to Ser372) (containing 20 amino acids at the 5¢ end of the DBD, the DBD and 40 amino acids at 3¢ end of the...

Ngày tải lên: 07/03/2014, 11:20

... [19], and papain [11], VS-A, VS-B and VXH-A lack the insertion of four amino acids between position 168 and 169, which is present in all other cysteine proteinases of plant or animal origin Unfortunately, ... cysteine proteinases The N-terminal amino acid sequences of the proteins VXH-I, VXH-III, VXH-IVa and VXH-IVb are shown in Fig 6, and are compared with the known N-terminal sequences of the cysteine ... cysteine proteinases from Caricaceae found in the GenBank database (Fig 1) These sequences included complete cDNAs from papain, glycyl endopeptidase, two isoforms of caricain and five isoforms of...

Ngày tải lên: 07/03/2014, 11:20

... redox-active cysteine, the resolving cysteine (Cys-SR) In typical 2-Cys, Sp at the N terminus and SR at the C terminus of the protein belong to different subunits and condense to form an intersubunit ... plasmid and a virus Mol Microbiol 34, 217–226 29 Bradford M (1976) A rapid and sensitive method for the quantification of microgram quantities of protein utilizing the principle of protein-dye binding ... the N terminus and SR at the C terminus belong to different subunits and condense to form an intersubunit disulfide bond; in atypical 2-Cys, Sp at the N terminus and SR at C terminus, originate from...

Ngày tải lên: 07/03/2014, 12:20

... (Met153 of the ML1p and ML2p B-chains and Met156 of the ML3p B-chain corresponding to the ricin B-chain Leu152; and Met234 of the ML1p and ML2p B-chains corresponding to the ricin B-chain Leu233) ... structure of preproricin [26,28] and mature lectins encoded by mlp genes Domain structure of the B-chain and position of the carbohydrate-binding sites are indicated Each B-chain domain is composed of ... Binding of the recombinant MLp A-chains with TA7 was detected in all samples Interaction of the native A-chains with TA7 and MNA4 in comparison with recombinant A-chains showed the same binding...

Ngày tải lên: 07/03/2014, 15:20

... Overexpression of HRT1 and HRT2 in E coli and purification of HRT2 The pETHRT1 and pETHRT2 were constructed and introduced into E coli BL21(DE3) The expression of these genes were induced by addition of 0.5 ... containing an 870 bp ORF, flanked by a 155 bp 5¢-UTR and a 254 bp 3¢-UTR including a poly(A) tail of 15 bp (Fig 1A) The cDNA of HRT2 was 1051 bp in length containing an 852 bp ORF (Fig 1B) and ... protein catalyzes the synthesis of new rubber molecules with the active involvement of a number of factors in WBP of fresh Hevea latex Total RNAs of rubber leaf and other tissues were obtained using...

Ngày tải lên: 07/03/2014, 21:20

... proteins from an in vitro transcribed RNA injected into X laevis oocytes High expression is obtained as a consequence of inserting the cDNAs between the 5¢- and 3¢-UTR of b-globin, a protein that ... expression of the Aa subunit of protein phosphatase 2A in human gliomas in the absence of mutations in the Aa and Ab subunit genes Int J Cancer 93, 798–804 31 Calin, G.A., De Lasio, M.G., Caprini, ... function of PR72 and PR130 is currently not known PR59 [26] and PR48 [27] were identified in a yeast two-hybrid assay as proteins interacting with p107 (a relative of the retinoblastoma protein) and...

Ngày tải lên: 08/03/2014, 08:20

... A., Inoue, K., Hiraoka, N., Ikeshiro, Y., Kushiro, T., Shibuya, M & Ebizuka, Y (2001) Cloning and characterization of a cDNA encoding b-amyrin synthase involved in glycyrrhizin and soyasaponin ... into pBluescript SK(–) (Stratagene) by in vivo excision, and was sequenced in both strands by the dideoxy chaintermination method using a model 373A DNA sequencer (PE Biosystems) Nucleotide and ... nucleotides in length, and contained an ORF of 2277 nucleotides A stop codon was present bp upstream of the initial ATG codon in the frame The cDNA sequence was divergent from those of LcCAS1 [13] and...

Ngày tải lên: 08/03/2014, 23:20

... (%), wind speed (m/s) at 13 o'clock and rainfall during the daylight in lowland area in term of assessment for development of tourism activities as well as existence of fog during the daylight in ... surface of human skin In the hot season, wind makes the process of sweat evaporation from skin easy However, strong wind in winter makes human body colder, strong and cold wind makes children and ... country, and their geographical location is presented in Table and Fig Note that within the framework of this paper, the term “Highland” is known as mountainous regions in Vietnam, including mountainous...

Ngày tải lên: 14/03/2014, 15:20

... referring to all of them as caveolae, was demonstrated by their content of both caveolin-1 and caveolin-2 The coexistence of caveolin-1 and caveolin-2 in all three caveolae subclasses is in line ... indicated protein or EZ-Link-sulfo-NHS-LC-biotin labelling by SDS ⁄ PAGE and immunoblotting or avidin-labelled HRP, respectively The amounts of indicated proteins are expressed in arbitrary densitometric ... densitometric units and normalized to percentage of maximum (A) Protein concentration (mgÆmL)1) (B) Caveolin-1 (C) Caveolin-2 (D) Cell surface biotin labelling of proteins (E) TGN38 (F) Insulin receptor...

Ngày tải lên: 16/03/2014, 13:20