... provides a rationale for assuming maturational delay. The Panel further notes that LOAELs of 500 and 200 mg/kg bw/day and NOAELs of 100 and 40 mg/kg bw/day from these studies are reasonably consistent, ... widespread public concern about the safety of phthalates. As part ofthe evaluation of phthalates, the CERHR convened a panel of scientic experts (Appendix I) to review, discuss, and evaluate the ... observed at gestational doses of 127–151 mg/kg bw/day and higher anda lactational dose of 166−377 mg/kg bw/day and higher; the developmental NOAEL is in the range of 38–44 mg/kg bw/day (gestational)...
... ofthe hydrolysis wasconfirmed by the disappearance ofthe (Met1)-ONC(M23L) signal in the MALDI-TOF mass spectra 1 h after the addition of AAP. The pH was adjusted to 2.5, 8.0 and 11.5 andthe ... Nitta, K., Kawauchi, H., Takayanagi, Y. & Oyama, F.(1991) Comparative base specificity, stability, and lectin activity of two lectins from eggs of Rana catesbeiana and R. japonica and liverribonuclease ... occurs in the pH range 8.0–8.5 [23]. When evaluating the influence of Table 2. Thermodynamic parameters ofthe thermal denaturaturation ofthe different onconase (ONC) variants at 25 C and pH 2.0....
... quantitative measurement ofthe activity of GABA-uptake, an aliquot ofthe stable CHO transfectants wasused for GABA-uptake assay, and another aliquot wasused to determine the quantity of ... 1631 The role of N-glycosylation in the stability, trafficking and GABA-uptake of GABA-transporter 1Terminal N-glycans facilitate efficient GABA-uptake activity of the GABA transporterGuoqiang Cai1,2, ... were analyzed on the basis of the MichaelisMenten equationV ẳ VmaxGABAẵGABAKmGABA ỵẵGABA and the parameters for NNN with and without treat-ment with dMM, and for N-glycosylation mutantDDN...
... among plants and cyanobacteria, the surface charge pattern variesgreatly [1]. Comparing cyanobacterial data with the wealth of information available for the higher plant reaction [2–5]reveals ... incyanobacteria and algae: an instance of unusual genetic-vari-ability. Biochim. Biophys. Acta 766, 310–316.7. Balme, A. , Herva´s,M.,Campos,L .A. ,Sancho,J.,DelaRosa,M .A. & Navarro, J .A. ... charge. The same analysis has been used for the interaction between Cyt f and plastocyanin [1], and for anin-depth discussion ofthe advantages and limitations of the Watkins model the reader...
... described the isolation and charac-terization of amphipathic a- helical peptides from the venom of Opistophtalmus carinatus, a scorpion living in southernAfrica, and we have made a comparative analysis ... melittin and mastoparan gave the same results. The role of LPS in the interaction was alsodemonstrated by the lack of effect of extracellular Mg2+on the activity ofthe peptides against Gram-positive ... [20],parabutoporin and opistoporin 1 were tested on Gram-negative and Gram-positive bacteria and their activity wascompared with the activity of melittin and mastoparan(Table 1).Parabutoporin...
... such as diethylstilbestrol, leads to squamous metaplasia ofthe prostate, a tissue change characterized by a multilayering of pros-tatic basal epithelial cells. Squamous metaplasia is associated ... estradiol has a clearer role in regulating male-typical brain and behavioral sexual differentiation in rodents compared to primates and humans. The sexual dimorphism of a number ofthe neural ... affected by the background incidence, e.g., tumor or malformation rates in control animals, variability ofa particular end- point, andthe magnitude ofthe effect. A sample size of at least...
... ofa federation of 11 states in Peninsular Malaysia andthe states of Sabah and Sarawak in the north of Kalimantan. Kuala Lumpur, the national capital, Labuan UNEP/SCS – National Report Malaysia ... part of South-East Asia and occupies a total land area of 330,434 square kilometres. The land mass comprises three main components: Peninsular Malaysia andthe two states of Sabah and Sarawak, ... tidal stream of over 4 knots. The West Coast of Peninsular Malaysia has an equatorial climate, with an average annual rainfall of more than 2500mm anda daily temperature that ranges from a...
... standard assay mixtures, the velocity of ABTS oxidationwas maximal at 45 °C. The dependence ofthe rate of ABTSoxidation by lac1 on substrate concentration at pH 3.0 and 45 °C followed Michaelis–Menten ... cycles of 94 °Cfor20s,52°Cfor20s and 70 °C for 2 min; then a final extension at 72 °Cfor 10 min. The primers for lac1 PLAC1F(5Â-AGCTTTCATTCCCAGTGATTG-3Â)andPLAC1R(5Â-AACGAGCTCAAGTACAAATGACT-3Â) ... product abundance was evaluated in the exponential phase ofthe reaction (half ofthe maximumproduct). To further validate the assays, the optimized cyclenumber (23 and 28 for gpd and lac1, respectively)...
... reviewer. M .A. Toscano acknowledges the assistance of R.P. Stumpf (NOAA/National Ocean Service) and K.S. Casey(NOAA/National Oceanographic Data Center) with SST data and climatology processing, and K. ... approach was used to analyze the quadrat data.Counts from the quadrats were pooled to obtain mean estimates of the abundance of juvenile corals and, separately, the abundance of E. viridis for each ... (Balistidae), the jolthead porgy Calamus bajonado (Sparidae), and the hogfish Lachnolaimus maximus (Labridae) – were436 Ag. tenuifolia was 50%; Aronson and Precht 1997), March 1999,February 2000, and...
... a KHdomain. RNA analysis and blast analysis indicatedthat homologs and orthologs of VBARP exist, indica-ting the presence of VBARP in diverse phyla such asplants, yeast, and eukaryotes. These ... repetitions of the yeast two-hybrid assay and using a mammalian hybridsystem (Invitrogen, Carlsbad, CA) (EDA Wheeler &V Ayyavoo, unpublished data). A blast searchrevealed that the IMAGE clone, ... VBARP-L consisted ofa 576-bp fragmentabsent from the shorter VBARP-S and contained a 114-bp UTR (Fig. 2A) . Intron and exon analysis of VBARP revealed that VBARP-L and VBARP-S con-tain 11 and...
... the adjacent Phe8Fig. 2. (/ – w)mapsofb-amino acids withindication ofthe conformational space commonwith that of a- amino acids. The (/ ) w)mapscorresponding to h-values of )60° and 60° areindicated ... proceduresMolecular mechanics calculationsN-acetyl NÂ-methyl amide derivatives of HGly, b2-HAla and b3-HAla andof a- amino acids were built usingINSIGHTII(Accelrys Inc.). Molecular mechanics calculations ... the torsion angles /, h and w. For the b2 and b3-amino acid substi-tuted-SP analogues, the methyl side chain of b-HAla exhibits the same orientation as the one in [Ala9]SP (CIP’s rule a- amino...
... coating, the HNE peptide was at least partially oxidized and the signal ofthe reduced species increased as a result of oxidation.Identification ofthe active isoformBecause of disulfide scrambling ... deathsoccur annually, making measles the most important cause of infant mortality worldwide. The low vaccine coverage and the low vaccine efficacy in the presence of maternalantibodies are major ... during an association time of 180 s anda dissociation time of 120 s at a constant ow rate of 20 lLặmin)1at 25 C. The signal ofthe control canalwith the irrelevant peptide was subtracted from the corresponding...
... side-chainsstrengthened heat or pressure stability. The mutation E45 0A and the sextuple mutation caused destabilization of the enzyme to pressure. Thermal inactivation data on mutantsshowed that all of them ... atP0 and 25 °C for the same periods of time (t), providing the control activity a 0at t0 and the residual activity a tattime t. The relative activity of AChE at P0 and 25 °Cfor the period of ... incubation of the partially denatured AChE, at 27 °C (data not shown).Table 2 depicts kdvalues of inactivation at 55 °C. The kdvalue ofthe E45 0A mutant is more than 10-fold lower thanthat of...
... on the vertex and one atom in the center ofthe bcc are localized in an elementary cell. Hence, the total number of atoms in an elementary cell is 2. Then if on average s is atomic number of ... linearly proportional to the temperature T. Therefore, at a given temperature T the quantity R defined by the ratio ofthe RMSF in atomic positions about the equilibrium lattice positions andthe ... equilibrium lattice positions andthe nearest neighbor distance reaches a critical value. Hence, the lattice thermodynamic theory is one of the most important fundamentals for interpreting thermodynamic...
... (Stratagene, La Jolla, CA,USA). The primers used were: 5Â-TATATCATTCAGGATTATTTGTATCTTTTAGAATACGCTAAGGTG-3Â (forward, the mutagenesis codon underlined) and 5Â-TTAGCGTATTCTAAAAGATACAAATAATCCTGAATGATATAAAAAC-3Â ... of degradative enzymes,such as the alkaline protease aprE, at the tran-scriptional level [6] and, for that reason, TenA is oftenclassified as ‘putative transcriptional regulator’ (http://au.expasy.org/). A ... was supported by the University of Padua and by the Italian Ministry for Research(COFIN 2007).Table 1. Statistics on data collection and refinement. A wavelength of 0.9794 A ˚was used. A charge-coupled...