defects in ankyrin based protein targeting pathways in human arrhythmia

... the WNV E protein was engineered to comprise a hexahistidine tag and a cysteine-containing linker at its C-terminus (DIII-C) Upon expression in E coli the protein formed insoluble inclusion bodies ... eliciting neutralizing antibody responses [12] In particular a structurally distinct domain of the E protein (DIII) has been proposed as the receptor-binding domain [13] Antibodies recognizing ... virus incorporating the envelope proteins of WNV into the infectious backbone of a yellow fever vaccine strain is currently being developed as a live-attenuated vaccine [9-11] While immunogenic in...

Ngày tải lên: 12/08/2014, 04:20

... doxycycline-inducible protein expression in human cell lines by just a single transfection For closely matching cellular backgrounds we use continuous expression of the original TetR instead ... in high numbers, even TetR fusion proteins based on VP16 minimal activation domains are toxic [12] Thus, relocation of TetR fusion proteins upon binding of inducer can be expected to cause secondary ... functional testing of individual clones In contrast, plasmids with an Epstein–Barr virus (EBV) origin of replication oriP in the presence of EBV-encoded EBNA-1 protein are continuously propagated in 1%...

Ngày tải lên: 23/03/2014, 09:21

... family is composed of three proteins, ankyrin repeat domain-containing protein (Ankrd2), cardiac ankyrin repeat protein (CARP) and diabetes-related ankyrin repeat protein (DARP), grouped together ... are involved in the transmission of mechanical signals to signaling pathways In the M line, mechanical stimulation activates the interaction of a protein complex with the kinase domain of titin, ... active calpain was investigated A direct, in vitro digestion of CARP by calpain using recombinant proteins could not be attempted because calpain is inactivated during purification [45] In skeletal...

Ngày tải lên: 29/03/2014, 21:20

... potent pro-inflammatory cytokines and chemokines The hall-mark cytokines involved in initiation of the adaptive Th1 immune responses are IL-12p70 whereas IL-6, TGFb and IL-23 are involved in initiation ... modest induction, and above 100 fold induction termed strong induction The induced cytokines and chemokines are shown in table Constitutively secreted proteins as seen for non-treated DCs included ... classified into weakly induced proteins (1-10 fold induced vs untreated), modestly induced proteins (10-100 fold induced vs untreated controls), and strongly induced proteins (more than 100 fold vs...

Ngày tải lên: 11/08/2014, 06:22

... determine the quality of SAM -based junctions For example, Rabson et al.10 found that pinholes present in tunneling barriers can mimic tunneling, and in this thesis we show that yields in non-shorting ... junctions are dominated by defects resulting in a sharp increase of J These large junctions suffered from Joule heating which indicates current concentrated at defect site With good understanding of the ... Understanding and Minimizing the Role of Defects in Self-Assembled Monolayer Based Junctions Jiang Li (M Sc Chemistry, Nanjing University, China) A THESIS SUMMITTED FOR THE...

Ngày tải lên: 09/09/2015, 08:18

... mechano-transduction, a collection of “hub” proteins, such as, α-synuclein, p53, 14-3-3, AXIN, are indispensible proteins which bind to a number of other proteins via protein- protein interactions [204207] When ... proline-containing regions are incapable of binding to proteins that form strand-edge protein interactions, such as the crystallin family of chaperones Proline is often found at the beginning ... Summary In signaling and mechano-related pathways, a type of protein domain is critical for transducing signals Such protein domains are located in the termini or flanked by folded domains, compositional...

Ngày tải lên: 09/09/2015, 10:14

... of tissue engineering to produce tracheal replacements and to examine these in vitro and in vivo Studies introduced by Vacanti et al in 1994 were trend-setting where constructs based on polyglycolic ... in ammatory reactions originating acute-phase reactions concomitant with the induction of gene expression of acute-phase proteins The concentrations of the acute-phase proteins α1-acid glycoprotein ... intubation over several days as an intratracheal splinting An external splinting by metallic microplates in the surgical tracheal reconstruction was described first time by Zalzal and Deutch in...

Ngày tải lên: 25/10/2013, 08:20

... lacking the Ca2+ ⁄ CaM-binding IQ domain, did not increase the basal or serotonininduced ERK1 ⁄ phosphorylation The ERK1 ⁄ phosphorylation was inhibited in the presence of the calcium in ux inhibitor, ... serotonininduced sustained increase in [Ca2+]i, as has been shown for endothelin-1-induced Ca2+ increase in Rat1 cells [29] Whether HEK293 cells express T-type Ca2+ channels, or whether the increase ... Schlessinger J (1995) Protein tyrosine kinase Pyk2 involved in Ca2+induced regulation of ion channel and MAP kinase functions Nature 376, 737–745 Dikic I, Tokiwa G, Lev S, Courtneidge SA & Schlessinger...

Ngày tải lên: 19/02/2014, 18:20

... center, founded in 1984 Since its founding, SFI has devoted itself to creating a new kind of scientific research community, pursuing emerging science Operating as a small, visiting institution, ... conceptually decomposing the system in single units, studying these units in isolation, and evaluating the whole as a linear combination of the units' properties (Lewontin and Levins 1987) A homogeneous, ... Jim Doran points out in chapter 5, agentbased approaches have the ability, in principle, to take into account rules, 14 Putting Social Sciences Together Again norms, differential learning contexts,...

Ngày tải lên: 14/03/2014, 21:20

... contain, in addition to similar Argonaute proteins, Dicer and other factors such as the transactivating response RNAbinding protein (TRBP) [11,12] Interestingly, it has also been demonstrated that ... failure in the processing of miR-430 [23] Interestingly, the MZdicer phenotype also included tail and MMB defects [23] Therefore, it seemed plausible that miRNA processing could be affected by the injection ... to inhibition of premiRNA processing by siRNAs competing for binding to Dicer, TRBP, and ⁄ or other limiting factors However, since our northern blot analysis did not reveal a concomitant increase...

Ngày tải lên: 16/03/2014, 06:20

... Fig TEM morphology of annealed SiNWs The inset is a SAED pattern Fig Typical HRTEM images of SiNWs: (A) an as-grown SiNW with defects; (B) an annealed SiNW without defects 348 Y.H Tang et al / ... fast growth of SiNWs, since it is well known that dislocations can accelerate crystal growth In other words, the defects in the tips of SiNWs are necessary for the growth of SiNWs However, the ... conclusion, the defects inside the SiNWs were signi®cantly decreased after the SiNWs were thermally annealed at 1100°C for h under an inert ambient HRTEM investigation of the microstructures in the nanowires...

Ngày tải lên: 16/03/2014, 15:03

... protein kinase C, Ca2+/calmodulin-dependent protein kinase II, and tyrosine phosphorylation are involved in carbachol-induced phospholipase D activation in Chinese hamster ovary cells expressing ... To determine whether PLC activity or G -protein- mediated signaling was involved in EGCG-induced PLD activation in U87 cells, we examined the effects of pertussis toxin and the phosphoinositide-specific ... pathway in many cell types [36], and the inhibitor attenuated EGCG-induced increases in PLD activity, PLC probably regulates PLD through stimulation of this kinase Interestingly, the increase in PLD...

Ngày tải lên: 16/03/2014, 16:20

... AKAP12 inhibits phosphorylation of PKCf in neighboring human retina microvascular endothelial cells (HRMECs), which leads to a decrease in VEGF levels and an increase in TSP-1 levels in HRMECs, ... angiopoietin-1 (Ang1) levels in CM under hypoxic conditions, and this played a role in barrier properties in HRMECs [20] Therefore, we examined whether the junction proteins claudin-1 and VE-cadherin ... manufacturer’s instructions Each stained area was presented relative to the area with the highest staining intensity Cell culture Primary human brain astrocyte cells, dissociated from normal human brain...

Ngày tải lên: 23/03/2014, 07:20

... mis-sense sequence variations, in addition to in uencing protein biogenesis, also may affect the splicing efficiency by interfering with binding sites for splice modulating factors [19] Although the ... MCAD proteins were assisted in their intramitochondrial folding by, first the chaperone heat shock protein 70 (Hsp70) and, subsequently, by chaperonin Hsp60, and that the K304E protein was retained ... folding of the variant protein is compromised Inspection of the molecular structure surrounding position 304 in the mature MCAD protein [41] indicated that the lysine at position 304 is in close...

Ngày tải lên: 23/03/2014, 12:20

... RIPPER is a fast rule induction algorithm It starts with splitting the training set in two On the basis of one half, it induces rules in a straightforward way (roughly, by trying to maximize coverage ... Memory -based learning techniques can be characterized by the fact that they store a representation of a set of training data in memory, and classify new instances by looking for the most similar instances ... M., (1999), Error spotting in human- machine interactions, Proc EUROSPEECH, Budapest, Hungary Litman, D., Pan, S (2000), Predicting and adapting to poor speech recongition in a spoken dialogue system,...

Ngày tải lên: 23/03/2014, 19:20

... AP-2 for binding to the regulatory region of the PAR-1 gene [19,29] Interestingly, in the human alveolar epithelial A549 cell line, Sp1 inhibition, as in human lung fibroblasts, did not in uence ... conditions Involvement of alternative cAMP-induced signaling pathways in the PGE2-induced downregulation of PAR-1 At present, two distinct cAMP-dependent signaling pathways are known The first one includes ... for 15 at °C The protein concentration was determined by the Bradford method (Bio-Rad Protein Assay; Bio-Rad), using BSA as standard Samples containing equal amounts of protein (30 lg) were separated...

Ngày tải lên: 30/03/2014, 04:20

... stringent washing, recombinant ATF-2 protein, a substrate for p38, was added to immunoprecipitates and incubated for 30 minutes according to manufacturer's specifications Phosphorylated protein ... ethanol in the presence of varying doses of IFNα HCV protein levels were analyzed by western blot 48 hours later As shown in Figure 6B, in the absence of ethanol, IFN-α inhibited HCV protein in a ... and this coincided with a dose-dependent increase in total Stat1 protein, a known ISG When cells were treated with a single dose of 100 mM ethanol, increases in HCV NS3 and NS5A proteins were detected...

Ngày tải lên: 19/06/2014, 08:20

... activities Protein concentration was determined in each S9 fraction according to the Bicinchoninic Acid Protein Assay (Sigma, France) Cell death measurement The enzymatic MTT test is based on the ... Séralini GE: Glyphosate -based herbicides are toxic and endocrine disruptors in human cell lines Toxicology 2009, 262:184-91 11 Urani C, Doldi M, Crippa S, Camatini M: Human- derived cell lines ... Luciferin Detection reagent (50 μl) was added before mixing for 10 s and incubating at room temperature for 90 in order to stabilize the luminescent signal The luminescence was then read with a luminometer...

Ngày tải lên: 20/06/2014, 00:20

... stringent washing, recombinant ATF-2 protein, a substrate for p38, was added to immunoprecipitates and incubated for 30 minutes according to manufacturer's specifications Phosphorylated protein ... ethanol in the presence of varying doses of IFNα HCV protein levels were analyzed by western blot 48 hours later As shown in Figure 6B, in the absence of ethanol, IFN-α inhibited HCV protein in a ... and this coincided with a dose-dependent increase in total Stat1 protein, a known ISG When cells were treated with a single dose of 100 mM ethanol, increases in HCV NS3 and NS5A proteins were detected...

Ngày tải lên: 20/06/2014, 04:20

... constant for each trap are given in Table This study reveals that the number of traps, including some electron emitting deep levels, increases with increasing Be-doping for the samples grown on (100) ... 360°C in H2/Ar mixture The deep level defects present in the samples were characterised electrically using DLTS and LDLTS techniques Results and discussion DLTS spectra shown in Figure are obtained ... with increasing Be-doping concentrations for (311)A samples These results are in agreement with the optical studies [7,10] where it was shown that superior PL efficiencies are obtained in Be-doped...

Ngày tải lên: 21/06/2014, 05:20