cystatin c as a biomarker in kidney disease

... A, Abatangelo G, Borrione A, Brocchetta D, Hollander AP, Kon E, Torasso F, Zanasi S, Marcacci M: Hyaluronan-based scaffolds (Hyalograft C) in the treatment of knee cartilage defects: preliminary ... quantitative PCR analysis ESJ participated in the design of the study and gave clinical cell culturing input LC isolated the biopsies and gave clinical feedback LP and MB provided critical clinical ... AGG TTAT-3', antisense 5'-GGA ACC ACT CTC ACC CTT CACA-3', probe 5'-TCC CTT AGC ACC GTC CAG GCC TG-3', were designed by using Primer Express Software version 2.0 (Applied Biosystems) All genes were...

Ngày tải lên: 09/08/2014, 06:22

... human PKC alpha was immediately added into the pre-warmed reaction mixture and incubated at 30 C for 30 minutes The incubation was terminated by placing the reaction tubes in a 95 C heat block ... DAGK Assay DAGK assay was established and described by Bollag and Griner et al (1998) In this assay, DAGK is incubated with its substrate (diacylglycerol), and a mixture of “cold” and radio-labeled ... was measured using the Bradford assay 2.1.2.1.4 Protein Quantification by Bradford Assay Bradford assay was used to quantify proteins in cell lysates It is a rapid and accurate method commonly...

Ngày tải lên: 11/09/2015, 16:06

... actaccatgagaattgcagtga RW: tcctcagaacttccagaatcag FW: ccacctggactacatcgg RW: tcctcatccctctcatacag FWD: aaccttagatgggggtgtcc RWD: gtggaagtgacgcctttca FWD: ccggtttatcaactggatgg RWD: tggatcgaggccagtaattc ... Lipoprotein lipase Leptin Primer sequence FW: atgctggctatgagcaggtc RW: gtgcagagacagcaggttca FW: ggaggaagctgtgaagatgc RW: gcacagcaacagtgagcagt FW: gcagagtccagcaaaggt RW: cagccattgatacaggtagc FW: actaccatgagaattgcagtga ... tggatcgaggccagtaattc FWD: tgacaccaaaaccctcatca RWD: atgaagtccaaaccggtgac Product size 101bp 139bp 70bp 70bp 109bp 123bp 145bp 102bp 3.1.4.3.2 PCR Standards Preparation Gel bands of the PCR products of the...

Ngày tải lên: 11/09/2015, 16:06

... expression of CD29, CD34, CD44, CD45, CD71, CD73, CD90, and CD105 Cell surface marker expression profile in human BM-MSCs was tested in passage 7, passage 8, and passage 9, and the cell surface marker ... Release Calcium is a very important second messenger which can trigger many cellular responses, including cell proliferation In this study, intracellular calcium release, in human BMand AD- MSCs, ... washes, and each wash lasting five minutes After that, the membrane was probed with a proper amount of secondary antibody for two hours, followed by another three 1xPBS washes, each wash lasting...

Ngày tải lên: 11/09/2015, 16:06

... therapeutic applications to balance the abnormal portions of osteoblasts and adipocytes observed in bone diseases such as osteoporosis and osteopenia, where the bone volume is decreased but the adipose ... obvious, as the cells are extracted from “unwanted” fat Also, the 156 Chapter Conclusion and Future Work procedure of extracting these cells is much less painful, compared with extracting MSCs from ... osteogenic/adipogenic differentiation The data described in chapter might also pave the way, for a more controlled and facilitate differentiation of stem cells, for future 154 Chapter Conclusion and...

Ngày tải lên: 11/09/2015, 16:06

... derivative in a variety of human cancer cell lines.” Int J Cancer: 66,358-366 Taha, T A. , Y A Hannun, et al (2006) "Sphingosine kinase: biochemical and cellular regulation and role in disease. " ... kinase mediates vascular endothelial growth factor-induced activation of ras and mitogen-activated protein kinases." Mol Cell Biol 22(22): 7758-68 Simmons, P J., B Masinovsky, et al (1992) "Vascular ... Mourcin, et al (2005) "Mesenchymal stem cells rescue CD34+ cells from radiation-induced apoptosis and sustain hematopoietic reconstitution after coculture and cografting in lethally irradiated baboons:...

Ngày tải lên: 11/09/2015, 16:06

... STEM CELL RESEARCH As was mentioned above, S1P can act as an intracellular second messenger and as an extracellular ligand for specific cell-surface receptors to regulate cell survival, in either ... substrate-sphingosine, into S1P S1P has dual functions On the one hand, it could act as an intracellular mediator and trigger numerous cellular events, including intracellular calcium release, and ... to be involved in various pathological processes SPHK activation generates S1P, which can function as an intracellular mediator, as well as an extracellular mediator by binding to its receptors...

Ngày tải lên: 11/09/2015, 16:06

... Wright AP (2005) c- Myc associates with ribosomal DNA and activates RNA polymerase I transcription Nat Cell Biol 7:303–310 Baena E, Gandarillas A, Vallespinos M, Zanet J, Bachs O, Redondo C, Fabregat ... (1997) Myc and Ras collaborate in inducing accumulation of active cyclin E/Cdk2 and E2F Nature 387:422–426 Leone G, Sears R, Huang E, Rempel R, Nuckolls F, Park C- H, Giangrande P, Wu L, Saavedra HI, ... Myc Proteins 335 activated by Myc, such as lactate dehydrogenase A (LDH -A) and ornithine decarboxylase: induction of these genes contributes to and can be required for Myc-induced proliferation...

Ngày tải lên: 25/10/2013, 21:20

... We have obtained data indicating that a- defensin-1 and a- defensins-2 induce an increase in the protein content of cyclin D and that quercetin inhibits the a- defensininduced increase in cyclin ... siRNA against luciferase mRNA was used as a control siRNA The target sequence for luciferase mRNA was 5¢-AACG TACGCGGAATACTTCGA-3¢ The siRNAs were customsynthesized by Qiagen (Valencia, CA, USA) ... Bachem (Torrance, CA, USA) Anti-collagen-I antibody was obtained from Novus Biologicals (Littleton, CO, USA) Antibodies against active ⁄ dephosphorylated b-catenin, total b-catenin and cyclin...

Ngày tải lên: 18/02/2014, 06:20

... of GC cultured on LN induced dramatic cell rounding and important functional changes such as a decrease in proliferation rate and an increase in pyknotic rate of GC, as well as a decrease in progesterone ... Agricultural and Scientific Research agencies (approval number A 37801) and conducted in accordance with the guidelines for Care and Use of Agricultural Animals in Agricultural Research and Teaching ... ovine GC spreading on LN and induced cell rounding These changes in cell shape were associated with a decrease in proliferation rate, an increase in cell death, a decrease in progesterone secretion...

Ngày tải lên: 05/03/2014, 17:20

... cervical cancer A TUNEL DAPI C3 3A Phase contrast Control Cisplatin DHEA B CASKI Control Cisplatin DHEA C HeLa Control Cisplatin DHEA 5602 Fig DHEA induces apoptotic death C3 3A (A) , CASKI (B) and ... cytotoxic than cytostatic Table Percentage of cells in each phase of the cell cycle as evaluated by flow cytometry Percentage of cells in the phases of the cellcycle G1 C3 3A CASKI HeLa DHEA induces apoptotic ... Authors Journal compilation ª 2009 FEBS ´ R A Giron et al DHEA and cervical cancer decrease was associated with an increase in the percentage of cells with a smaller amount of DNA in the so-called sub-G1...

Ngày tải lên: 16/03/2014, 00:20

... pathways [67] The role of caspases in GPCR-induced cell survival is further confirmed by the ability of the peptide hormone apelin to decrease the activation of caspase 9, as well as caspases and In ... promote the activation of protein kinase C and the mobilization of intracellular Ca2+, leading to the formation of a complex containing Src-family kinases, the focal adhesion kinase, Pyk2, and PI3K ... employed in connecting GPCR-initiated signals to downstream events regulated by Akt ›, indicates an increase in protein levels or activity; fl, indicates a decrease in protein levels or activity AC, adenylyl...

Ngày tải lên: 16/03/2014, 05:20

... AACGGATTTGGCCGTAT-3¢, antisense: 5¢-AGCCTTC TCCATGGTGGTGAAGAC-3¢); c- fos (sense: 5¢-GAATA AGATGGCTGCAGCCAAGTGC-3¢, antisense: 5¢-AAG GAAGACGTGTAAGCAGTGCAGC-3¢), and c- myc (sense: 5¢-AAGTTGGACAGTGGCAGGGT-3¢, ... were examined Their selection was based on information that each pathway is known to activate one of the key transcription factors that regulates c- fos promoter activity MAP kinase and PI3-kinase ... arg-mART (1.35-fold), however, the increase in cytosolic activity was only marginal (Fig 3B) Activation of arginine-dependent mART would be expected to lead to an increase in the post-translational...

Ngày tải lên: 17/03/2014, 09:20

... GCC CTG TCC CGA TGC CTG CCC Size GAC) ACC) TTC GAC GGC ATA GCC TTC GGC GTA AAT CCT AGG TGC CTA GGC GAA GGC CTA GGC GCA CTA ATG AAG TGG CCA GCA TCG CGG CCA ACA GAT GCC AGG AGG GCC GCC GTC GGG GCC ... DP8S739 AFor DPP8S739 ARev DP9RGDmut.For DP9RGDmut.Rev DP9S729 AFor DP9S729 ARev Nucleotide sequence ATT CGA ATA GGG TAT TCT TAT CCC (GTC (GGT GAA GTC CCA GTA GCC GGC CCA GAA CGG TCC GAC GGC TGG GCC ... TGG ATC ACC TAT ATA GTG GCC GGC CTT ATG CAG ACT ACC TCC CAG CAA TG TTT AGA GC GGG TGT AG C G C G 35 35 30 32 mer mer mer mer 25 25 24 24 25 25 mer mer mer mer mer mer monoclonal antibody (Invitrogen)...

Ngày tải lên: 23/03/2014, 11:20

... of caspase inhibition on lovastatin-induced protein cleavage and neuronal apoptosis C Fig Effect of lovastatin alone or in combination with mevalonate on caspase-3 activation in immortalized rat ... that lovastatin-induced caspase-3 activation in neuronal cells may be cell type speci c To examine the contribution of caspases to cleavage of focal adhesion proteins and PKCd induced by lovastatin, ... indicated concentrations of lovastatin, mevalonate and caspase inhibitors and the incubation was continued for a further 24 h Cell morphology was analyzed by phase contrast microscopy in a Leica...

Ngày tải lên: 23/03/2014, 11:20

... question was now examined on invasive squamous cervical carcinomas and HeLa cells using our highly speci c antiPP17 antibody In fixed embedded tissue sections of squamous cervical carcinoma, mainly ... differentiation Our previous data on the oncodevelopmental overexpression of PP17b in prematurely aging epithelial-character placentas and squamous epithelial cervical dysplasias and carcinomas indicated ... (A) In invasive squamous cervical carcinoma, tumour cells have punctuated, ring-like cytoplasmic PP17 staining (immunohistochemistry, haematoxylin counterstain) (B) Lipid-loaded HeLa cells have...

Ngày tải lên: 23/03/2014, 21:20

... surface area across which intermolecular forces act, and it may induce microstructural changes (possibly increased crystallinity) in the cured adhesive, both of which may act to increase joint ... silica treated with various silane coupling agents to varying degrees of coverage Surface energy decreased with coverage in all cases, reaching a final minimum value for each silane when coverage ... a given case Any given real case often entails a combination of mechanisms 1.3.1 Adsorption mechanism (contact adhesion) In the adsorption mechanism, adhesion is modeled as occurring across a...

Ngày tải lên: 26/04/2014, 15:36

... grown into nearby areas, we speculated that CK 2a may participate in CRC cell invasion Figure Immunohistochemical detection of CK 2a expression in colorectal cancers, adenomas and adjacent normal colorectal ... purposes The CRC stage was defined according to the AJCC classification The clinical characteristics of the patients with CRC are summarized in detail in Table The tumors taken from the adenoma group ... investigated the association between CK 2a expression and the clinicopathological characteristics of CRC cases and found that CK 2a overexpression was significantly associated with T classification (P =...

Ngày tải lên: 18/06/2014, 19:20

... seaweed It is associated with loss of mammary myoepithelial cells in tissue culture provides a potential mechanism for increasing invasive mammary carcinoma [5] and induced colonic neoplasia in ... forty lymphocyte cultures in each borax concentration was carried out in duplicate fashion Mean absorbance was calculate for the control wells and for each borax concentration in the test wells ... well and incubated for hours at 37 C The medium was removed and formazan was dissolved in DMSO and the optical density was measured at 570 nm using a Bio-assay reader MTT assay of forty lymphocyte...

Ngày tải lên: 20/06/2014, 00:20