culture lasv propagation rna preparation cdna synthesis

... early diagnosis and disease progression in hemorrhagic fevers Methods Cell culture, LASV propagation, RNA preparation, cDNA synthesis HEK-293T/17 cells (ATCC CRL11268) were maintained in complete ... secretion of sGP2 from LASV GPC deletion Expression and secretion of sGP2 from LASV GPC deletion variants Cell extracts and supernatants from HEK-293T/17 transfected with LASV GPC deletion variants ... citation purposes) Virology Journal 2008, 5:161 13 mL of cEMEM was added, and the culture was incubated in a similar manner for 96 h To prepare total cellular RNA, the cell culture medium was replaced...

Ngày tải lên: 12/08/2014, 04:21

... partners  Adenine-Thymine  Cytosine-Guanine Just a note about RNARNA is single-stranded and acts as a code for protein synthesis  RNA is still made of nucleotides that have a phosphate, a sugar, ... ribosomes  DNA and protein synthesis, then, happens this way:  DNA sequence codes (how letters are put together) produce messenger RNA sequence codes  The messenger RNA leaves the nucleus and ... it’s found in the nucleus of eukaryotic organisms  How does DNA code for protein synthesis? First-what is “protein synthesis ?  It’s building, or assembling, a protein molecule from amino acids...

Ngày tải lên: 13/03/2014, 16:36

... the HCV minus-strand RNA in vitro and that deletion of the 3¢ 45 nt greatly impaired RNA synthesis [17] Oh et al [18] showed that RNA synthesis from the HCV minus-strand RNA required a minimum ... reduced RNA synthesis (Table 1) In all cases RNA synthesis was increased Altogether, these results indicated that while the presence of the SL-A1 domain is necessary for efficient RNA synthesis, ... Analysis of RNA synthesized in one round of synthesis RNA products shown in Fig were obtained under conditions where HCV NS5B could reinitiate RNA FEBS Journal 272 (2005) 3872–3886 ª 2005 FEBS synthesis...

Ngày tải lên: 20/02/2014, 01:20

... nitrogen by means of mortar and pestle for RNA extraction RNA isolation Total RNA from different shrimp tissues was extracted using RNAgents Total RNA Isolation System (Promega, Madison, WI, ... double-stranded RNA To investigate the ability of dsRNA to disrupt the CHH function in adult shrimps, cDNA corresponding to the CHH mature peptide was used as template for synthesizing dsRNA in vitro ... dsRNA into the abdominal cavity showed a significant decrease FEBS Journal 273 (2006) 5669–5677 ª 2006 The Authors Journal compilation ª 2006 FEBS J M Lugo et al Functional analysis of CHH by RNAi...

Ngày tải lên: 23/03/2014, 10:20

... viral RNA synthesis As T7 RNA polymerase was able to elongate self-complementary RNA templates by template-directed RNA synthesis during in vitro transcription [30], both (+)3¢-UTR and (–)IRES RNA ... TTCGACGTGA-3¢ RNA quantification was performed as follows: the precipitated RNA was reverse transcribed to cDNA using P2 as a primer, then lL of cDNA was used Ó FEBS 2003 De novo RNA synthesis by ... activity Evidence of de novo RNA synthesis with either plusor minus-strand viral RNA as template For the positive strand RNA viruses, the mechanisms of initiating viral RNA synthesis are rather different...

Ngày tải lên: 30/03/2014, 20:20

... antisense RNA amplification proced cDNA PREPARATION [1] to the primer/template [oligo(dT)24-T7/mRNA] complex and copies singlestranded m R N A into cDNA, such that each cDNA contains a T7 RNA polymerase ... RNA (ssRNA) that connects the biotinylated cap and the truncated c D N A mRNA hybrid (Fig 1C) This ssRNA can be digested by RNase I, an ssRNA-specific ribonuclease, in the case of truncated cDNAs, ... end(cap) and the 3' end of the mRNA (C) Strategy for the preparation of the cDNA library FL, Full-length first-strand cDNA: NFL, non-full-length cDNA 22 cDNA PREPARATION [2] it possible to synthesize...

Ngày tải lên: 10/04/2014, 11:12

... Virology Journal 2007, 4:105 ent RNA polymerase L [6,10] The complex of VP35 and L acts as the active RNA- dependent RNA polymerase with VP35 serving as polymerase ... intracellular inclusions, interaction of NP with VP35 and for the function of NP in RNA synthesis Materials and methods Cells and cDNA transfections HeLa, HUH7 and HUH-T7 cells [13] were grown in Dulbecco's ... MARV RNA It is possible that the conformational flexibility of NP, which allows for both intra- and intermolecular binding, is a prerequisite for NP to perform its multiple tasks in RNA synthesis...

Ngày tải lên: 18/06/2014, 18:20

... Virology Journal 2005, 2:86 http://www.virologyj.com/content/2/1/86 A 16000 14000 no vRNA no vRNA [ 35 S] CTP (cpm) 12000 vRNA vRNA 10000 vRNA + 3CD vRNA + 3CD pro (3Cpro H40A) 8000 vRNA + 3C vRNA + ... (pfu/µg vRNA) 1.E+07 1.E+06 vRNA vRNA vRNA + 3CD(3C proH40A) vRNA + 3CDpro 1.E+05 pro vRNA+3CDpro (3C(3C vRNA + 3CD R84S/I86A) R84S/I86A) vRNA ++ 3CD(3Cpro H40G,3D pol R455A/R456A) 3CDpro vRNA (3D ... http://www.virologyj.com/content/2/1/86 A vRNA + SDS vRNA + 14000 SDS 80S [35 S]methionine (cpm) 12000 vRNA vRNA 10000 vRNA + 3CDpro(3Cpro H40A) vRNA + 3CD + 8000 SDS 6000 pro H40A) vRNA + 3CD 3CD vRNA + pro (3C + SDS 4000 vRNA +...

Ngày tải lên: 19/06/2014, 08:20

... Virology Journal 2007, 4:105 ent RNA polymerase L [6,10] The complex of VP35 and L acts as the active RNA- dependent RNA polymerase with VP35 serving as polymerase ... intracellular inclusions, interaction of NP with VP35 and for the function of NP in RNA synthesis Materials and methods Cells and cDNA transfections HeLa, HUH7 and HUH-T7 cells [13] were grown in Dulbecco's ... MARV RNA It is possible that the conformational flexibility of NP, which allows for both intra- and intermolecular binding, is a prerequisite for NP to perform its multiple tasks in RNA synthesis...

Ngày tải lên: 20/06/2014, 01:20

... Figure Schematic diagram of the synthesis system for carbon/water nanofluid (a) The synthesis system for carbon/water nanofluid (b) The vaporization chamber in the synthesis system Teng et al Nanoscale ... Teng et al.: Preparation and characterization of carbon nanofluid by a plasma arc nanoparticles synthesis system Nanoscale Research Letters 2011 6:293 Submit your manuscript to a journal and benefit ... material properties Preparation of carbon/water nanofluid by plasma arc The carbon/water nanofluid in this study was prepared by the plasma arc system [19], which belongs to onestep synthesis system...

Ngày tải lên: 21/06/2014, 04:20

... concerns the possibility of applying the IMS technique not only for the preparation of zerovalent metal NPs but also for the synthesis of INPs of low solubility compounds (e.g metal sulphides) using ... Inter-matrix synthesis of polymer stabilised metal nanoparticles for sensor applications Contrib Sci 2005, 3(1):19 21 Ruiz P, Muñoz M, Macanás J, Turta C, Prodius D, Muraviev DN: Intermatrix synthesis ... membrane as nanoreactor: Intermatrix synthesis of platinum-copper core-shell nanoparticles Reac Funct Polym 2007, 67(12):1612 23 Muraviev DN, Ruiz P, Muñoz M: Synthesis, stability and electrocatalytic...

Ngày tải lên: 21/06/2014, 04:20

... were cultured in 100 ml flasks containing 20 ml of nutrient medium Each treatment involved 30-60 explants and was repeated twice Culture media and conditions Organ cultures Explants were cultured ... can be used for tissue culture propagation of oak: axillary shoot multiplication and somatic embryo- genesis Micropropagation by axillary shoot multiplication To establish cultures, we used actively ... used for mass propagation of selected oak clones; for example, micropropagation may provide the initial multiplication stage prior to stemcutting propagation Chalupa V (1984) In vitro propagation...

Ngày tải lên: 08/08/2014, 19:21

... cytokines like granulocyte macro- Tumor RNA preparation LNCaP cells cultivated in RPMI-1640 with 10% FBS were harvested and maintained at -80°C until RNA extraction The RNA was extracted with Trizol (Invitrogen, ... vaccine preparation have been described [6-8] For prostate cancer, DCs are most commonly primed with entire or partial tissuespecific antigens or tumor-associated antigens Alternatively, mRNA molecules ... 6:2 http://www.gvt-journal.com/content/6/1/2 USA) following the supplier's recommendations RNA concentration and purity were estimated in a 260/280 nm spectrophotometer RNA integrity was verified...

Ngày tải lên: 14/08/2014, 19:22

Ngày tải lên: 13/03/2015, 10:11

... of literature that ‘forced writers to see that the possibility of alternative readings merged with the possibility of alternative social orders’.23 Whereas part concentrates on the dominant arguments ... Biographia Literaria; gender relations in A Vindication of the Rights of Woman and Don Juan; journalism by Cobbett and Hazlitt; peotic form, content and style by the Lake School and the Cockney ... a complete list of titles published see end of book THE CRISIS OF LITERATURE IN THE 1790s Print Culture and the Public Sphere P AU L KE E N         ...

Ngày tải lên: 21/09/2012, 11:00

... adverted to the concept of Kultur to explain this phenomenon, speaking of ‘ culture layers,’’ ‘ culture ground,’’ and ‘ culture earth.’’ The ground’s speciWc nature demonstrated for them a shallowness ... Everywhere were people whose surnames were messes of ethnicity (or living testimony to the accretion of history and identity, depending on one’s view) Even so, surnames were not reliable indicators ... the military history.4 For an understanding of the role of the East in German war aims and internal politics, the appearance of Fritz Fischer’s GriV nach der Weltmacht in 1961 and the explosive...

Ngày tải lên: 21/09/2012, 11:02

... Many people believe that television damages culture It promotes the stronger cultures of countries such as Britain and North America and weakens the cultures of less wealthy countries This is ... and international students living in Australia, can watch programs from their own culture on the television In conclusion, I hold the view that television promotes and strengthens those cultures ... acknowledge that television does have some positive effects on the cultures within a society as well People who not live within their own culture can, in a limited way, access it through the multicultural...

Ngày tải lên: 04/10/2012, 09:39

Ngày tải lên: 04/10/2012, 10:24

Ngày tải lên: 04/10/2012, 10:24