crystal violet cell viability assay

... CONFOCAL MICROSCOPY ANALYSIS 62 3.22 CRYSTAL VIOLET CELL VIABILITY ASSAY 62 3.23 CELL CYCLE ANALYSIS BY PROPIDIUM IODIDE STAINING 63 3.24 COLONY FORMATION ASSAY 64 3.25 TERMINAL RESTRICTION FRAGMENT ... and PIAS 84 Figure 4.13 Crystal violet cell viability assay indicates that the over-expression of the indicated plasmids individually enhances viability while reducing viability in combination ... JFCF-6/T.1R cells 91 Figure 4.18 JFCF-6/T.1R cells were synchronised in each phase of the cell cycle 95 Figure 4.19 SUMO-p53 is enhanced in G0 and M phase of the cell cycle of JFCF-6/T.1R cells 96...

Ngày tải lên: 11/09/2015, 10:18

... cancer cell line CKBM is found to inhibit cell proliferation through the induction of apoptosis and G2/M cell cycle arrest To further elucidate the mechanism of CKBM on apoptosis and G2/M cell ... Technologies, Inc., CA, USA) at % CO2, 37 ℃ Cell proliferation assay (BrdU) Cells were seeded in a 96-well flat bottom plate at a density of x 104 cells/well After 24 hr, 20 µl of CKBM (Batch ... Staining AGS cells were seeded in a 6-well plate at a density of 1.2 x 106 cells/well After 2, or hr of CKBM treatment at a concentration of 5, 10 or 15 % v/v, cells were trypsinized Then cells were...

Ngày tải lên: 02/11/2012, 11:12

... distribution, or does not have the starting cells mimic a particular cell (with respect to DNA content, cell size and cell composition) of a particular age, then the cells are not synchronized A complete ... of cells should be narrow in the synchronized cells and these distributions should then reflect the movement of cells through the division cycle Thus, newborn cells should be essentially pure cells ... synchronized cells should be narrower than the size distribution of the original population, cell size should increase as the cells move through the cell cycle, and during the period of cell division...

Ngày tải lên: 13/08/2014, 23:20

... translocation experiments, cells were removed with 0.05% trypsin EDTA, and plated with cell solution of ã 106 cellsặmL)1 (in a 50 mL ask) and grown until 80% conuency Cellular assays A549 cell solution (100 ... containing IKK2 inhibitor) of IL-1a was prepared for cell assay and was added following the same procedure described for the time course cell assay The cells were washed with NaCl Pi and xed after ... in IL-1a-stimulated A549 cells To test whether these in vitro data were also found in in vitro cell cultures, a nuclear NF-jB translocation assay was performed where the cells were pretreated with...

Ngày tải lên: 16/03/2014, 11:20

... FEBS 2004 Cell- free synthesis of 15 N-labelled proteins (Eur J Biochem 271) 4089 4090 K Ozawa et al (Eur J Biochem 271) Fig (Continued) Ó FEBS 2004 Ó FEBS 2004 Cell- free synthesis of The cell- free ... the k promoters, however, cell- free synthesis of hCypA was below the detection limit of SDS/PAGE with Coomassie blue staining, even though the same plasmid produced excellent yields in vivo (data ... different proteins Ó FEBS 2004 Cell- free synthesis of 15 N-labelled proteins (Eur J Biochem 271) 4087 Optimization of other conditions for in vitro protein synthesis Fig Cell- free synthesis of PpiB...

Ngày tải lên: 16/03/2014, 18:20

... elutriated cells The cell cycle distribution of the cell fractions is shown in Fig 5219 Cell cycle regulation of mouse cyclin mRNAs A Penelova et al Fig Cell cultures of mouse EL4 cell fractions ... during the cell cycle The moderate regulation cannot be attributed to a lack of cell synchronization or cell viability (Fig 2) Two independent methods, elutriation and cell sorting gave excellent ... cyclin mRNAs in enriched cell cycle fractions of mouse EL4 cells Cells were separated either by elutriation or by cell sorting into G1, S and G2 ⁄ M phase fractions (A) Cells were separated by...

Ngày tải lên: 30/03/2014, 20:20

... ApoG2 was suspended in 0.5% sodium carboxymethylcellulose and prepared on the day of use MTT Assay NPC cell viability was assessed using an MTT assay based on mitochondrial conversion of MTT from ... cycle arrest in prostate cancer cells and colon cancer cells [8,9] To determine whether ApoG2 could also induce cell cycle arrest in NPC cells, we performed a cell cycle analysis using flow cytometry ... obvious cell apoptosis in NPC cells and little cells were accumulated in sub-G1 phase Instead, ApoG2 induced cell cycle arrest at the DNA synthesis (S) phase in a large percentage of NPC cells...

Ngày tải lên: 18/06/2014, 15:20

... steps The eukaryotic cell- cycle process is an ordered sequence of events by which the cell grows and divides in two daughter cells It is organized in four phases: G1 (the cell progressively grows ... going through the cell cycle A number of check-point modules, across cell cycle, regulate cell growth and genome replication during the sequential G1 , S, and G2 phases and cell duplication via ... similar to those observed in real cell- cycle control systems During cell- cycle progression, families of genes have either brief or sustained expression during specific cell- cycle phases or transitions...

Ngày tải lên: 22/06/2014, 19:20

... http://genomebiology.com/2010/11/2/R17 Page of 19 (b) Cell number Cell number Cell number 2C DNA content DNA content DNA content %S DNA content %G2-M DNA content t=12 t=10 Cell number Cell number t=8 Cell number t=6 %G1 t=5 ... transcript expression during the cell cycle To synchronize cell division in P tricornutum, we subjected exponentially growing cells to a prolonged dark period, which arrests the cells in the G1 phase [32] ... G1 phase identity of cells containing a single chloroplast When cells were released from the dark arrest, the population of bi-chloroplastidic cells steadily increased and cells entered the S...

Ngày tải lên: 09/08/2014, 20:21

... Figure of Control1 cell growth and viability in HL-60 cells by LA Control of cell growth and viability in HL-60 cells by LA (A) Cells were treated with 0, 1, 2.5 and mM LA and the cell numbers were ... analyses Cell cycle analysis Cell cycle phase distribution was assayed by flow cytometry Following 24 and 48 h treatment of HL-60 cells with different concentrations of LA (0, 2.5, and mM), cells ... times, control and treated cells were harvested Cell count was performed using a hemocytometer and cell viability was determined by trypan blue exclusion [25-27] Harvested cells were washed twice...

Ngày tải lên: 10/08/2014, 22:20

... Phase of the Cell Cycle To monitor the initiating event of cellular signaling for Vpr-induced G2 arrest, we adopted a single cell cycle assay to measure this event in a synchronized cell population ... host cell cycle regulation and HIV biology Methods Cell Line, Cell Cycle Synchronization and Adenoviral Transduction HeLa cells were grown in Dulbecco's modified Eagle's medium (DMEM) (Cellgro) ... vpr-expressing and HU-treated cells (Figure 3A, second row, lanes 57 and lanes 11-13) Cell cycle profiles were monitored by flow cytometric analysis While untreated cells had normal cell cycle progression,...

Ngày tải lên: 13/08/2014, 01:20

... changes in amplitude may reveal effects of cell- arrest methods on the expression of some cell cycle related genes In a single cell, the amplitude and phase of a cell cycle related gene are considered ... of cell cycle related genes More quantitative measures of cell culture synchrony, and investigation of the impacts of cell culture with various degrees of synchrony on expression of some cell ... to arrest cells in genome-wide cell cycle studies [10-13] Each method may have direct or indirect impacts on the synthesis or degradation of mRNAs from some genes after the interrupted cell cycle...

Ngày tải lên: 13/08/2014, 23:20

... and cell cycle genes Cdc7, Cdk2, Cdk2ap1, and Cdk4, which are consistent with the exit from cell cycle that accompanies maturation of early DP cells, which are cycling, into later DP and SP cells, ... antigen in thymic medullary epithelial cells, triggering negative selection at the physiological stage during the transition from cortical DP cells to medullary SP cells Unlike thymocyte apoptosis ... selection (S+) were sorted from TCR transgenic mice as CD4+CD8lowCD69+1G12+ cells Early single positive cells with an identical cell surface phenotype but beginning to undergo negative selection (S-)...

Ngày tải lên: 14/08/2014, 17:22

... simple unicellular bacteria to complex multicellular animals, revealed that this process involves duplicating cellular contents followed by division into two identical cells (Nurse 2000a) Cell cycle ... uncovered in p27Kip1 -deficient cells Curr Biol 9:163–173 Conlon I, Raff M (2003) Differences in the way a mammalian cell and yeast cells coordinate cell growth and cell- cycle progression J Biol ... the knowledge of the cell and its vital processes J Cell Biol 25:1–69 Follette PJ, O’Farrell PH (1997) Connecting cell behavior to patterning: lessons from the cell cycle Cell 88:309–314 Follette...

Ngày tải lên: 07/10/2012, 16:51

... aging Indeed, while cells, in this case neurons, have the capacity to maintain homeostatic balance and function under condition of stress, several “hits” may disrupt the cells’ regeneration capacity ... BRCA1 in neurons in AD may represent an attempt towards homeostasis by the cell, working with other factors to halt the cell cycle and mediate DNA repair Interestingly, a much higher proportion ... specifically and intensely localized with intracellular NFT in hippocampal neurons in AD (Figure 1A) In young control cases and those without any tau pathology, no cellular staining was seen (Figure 1B)...

Ngày tải lên: 26/10/2012, 10:04

... protein that promotes cell survival and has a role in maintenance of reproductive germ cells The knockout leads to progressiveloss of germ cells, Sertoli cells and Leydig cells and cause extensive ... yeast, 151 cell cycle engine, 4, – MPF, cell cycle theory, 1, 19 – cell division, – development of, – future of, 23 cell division, 230, 272, 273, 275, 282, 293, 294, 305, 307, 311, 353 cell division ... mediating cell cycle regulators, growth factors and intracellular communication pathways for germ cell development Cytoplasmic and Apoptotic Factors During gametogenesis, apoptosis of germ cells...

Ngày tải lên: 25/10/2013, 21:20

... Chx10-Cre leads to the loss of distinct neuronal cell types (photoreceptors for α-Cre, ganglion cells, photoreceptors and bipolar cells for Nestin-Cre or rod cells for Chx10-Cre) without the development ... R (2004) Cell- specific effects of RB or RB/p107 loss on retinal development implicate an intrinsically deathresistant cell- of-origin in retinoblastoma Cancer Cell 5:539–551 Modeling Cell Cycle ... somatic cell hybridization experiments between normal and transformed cells resulted in phenotypically normal hybrids that often reverted to being transformed, indicating the existence of cellular...

Ngày tải lên: 25/10/2013, 21:20

... one round of cell division, every cell in metazoans has to decide whether it will re-enter the cell cycle, exit the cell cycle and enter a quiescence state, and every quiescent cell has to similarly ... a set of information processors (cell cycle machinery) that integrate extracellular and intracellular signals to coordinate cell cycle events In order that a cell can produce an exact duplicate ... within certain cell types such as trophoblast giant cells of the placenta and megakaryocytes, but it can also be observed in cells due to a defect in the cell cycle regulation of these cells For example...

Ngày tải lên: 25/10/2013, 21:20

... Therefore, germline disruption of p16INK4a in human cells appears to affect senescence more significantly than in mouse cells Mouse Cells vs Human Cells: Roles of Reactive Oxygen Species and Telomere ... (1995) The role of Rb in cell cycle control Prog Cell Cycle Res 1:9–19 268 H Kiyokawa Hayflick L, Moorhead PS (1961) The serial cultivation of human diploid cell strains Exp Cell Res 25:585–621 Herbig ... and cell mortality Cell 85:27–37 Serrano M, Lin AW, McCurrach ME, Beach D, Lowe SW (1997) Oncogenic ras provokes premature cell senescence associated with accumulation of p53 and p16INK4a Cell...

Ngày tải lên: 25/10/2013, 21:20

... critical regulator of most cell cycle responses While each single pocket protein can mediate cell cycle arrest upon cell- cell contact, pRb is the critical mediator of cell cycle arrest upon growth ... roles in controlling cell cycle exit upon growth-inhibiting signals Only ablation of all pocket proteins fully alleviated a cell cycle arrest upon serum withdrawal, cell- cell contact inhibition, ... either cause cell cycle arrest or sensitize cells to apoptosis The behavior of triple knockout cells indicates that this decision depends on pocket protein functions In their presence, cells arrest;...

Ngày tải lên: 25/10/2013, 21:20