cryopreservation and revival of mesenchymal stromal cells

... differentiate from mesenchymal stromal cells (MSCs) MSCs are multipotent cells that can replicate as undifferentiated cells and that have the potential to differentiate to lineages of mesenchymal tissues, ... distribution of mCRY1 and mPER2, and real-time PCR quantification of mCRY1 mRNA after laser irradiation Intracellular distribution of mCRY1 and mPER2 after blue laser irradiation were shown in Figs 1a and ... accumulation of lipid droplets in cells (B) mRNA levels of PPARγ in MSCs 24 hr after laser irradiation (100 mW/cm2) and in non-irradiated cells Samples were normalized to mRps18 The mRNA levels of PPARγ...

Ngày tải lên: 08/08/2014, 17:20

... field of view was performed to enable identification of GFP-positive cells and immunopositivity for matrix proteins in the same cells Results Identification of injected mesenchymal stem cells ... induce the differentiation of MSCs to a disc-like phenotype and direct the cells to form a new and appropriate matrix Materials and methods Mesenchymal stem cell source and extraction Bone marrow ... identify the presence of apoptotic cells; for immunofluorescence and immunohistochemistry for aggrecan, Sox-9 and types I, II and X collagen to assess phenotypic characteristics; and for histochemistry...

Ngày tải lên: 09/08/2014, 01:22

... immunosuppressive activity of standard MSCs despite the appearance of a small number of senescent-like cells Materials and methods Production of MSCs and SB623 cells MSC and SB623 cells were produced ... cells having various features of regulatory T cells SB623 cells are derived from NICD-transfected MSCs and expanded in the absence of exogenous cytokines As such, SB623 cells retained the standard ... potency of naïve marrow stromal cells and Notch-transfected marrow stromal cells Journal of Neuroinflammation 2011 8:133 Submit your next manuscript to BioMed Central and take full advantage of: ...

Ngày tải lên: 19/06/2014, 22:20

... tnecseroulf gniwollof ehT SBP htiw semit eerht desnir erew selpmas ehT Co73 ta h rof sllec eht htiw detabucni erew seidobitna yramirp ehT ylevitcepser ,PAFG rof 002 : dna ESN rof 002 : ta ares ... 001-X notirT %4.0 neht dna ,nim rof enoteca %001 ,nim 01 rof lonahtem %001 dloc eci htiw detaert neht erew yehT erutarepmet moor ta nim 01 rof SBP ni edyhedlamrof %7.3 htiw dexif neht dna SBP ... llec gnimrofrep dna esu lacinilc rof SBF gnisu tuohtiw oviv xe sCSM dnapxe ot sdohtem dnif ot deen ew dna ,hcraeser ruo rof SBF desu ew ,yduts tneserp ni ,revewoH noitarefilorp sCSM rof noitidnoc...

Ngày tải lên: 07/08/2014, 18:21

... top, and the image was digitized and stored (Eclipse TE2000-E, Nikkon; MetaMorph 7.6.1.0 software) The number of cells that migrated to the lower side of the membrane was counted by means of light ... the repair of many tissues by mesenchymal stem/multipotent stromal cells (MSCs) Clin Pharmacol Ther 2007, 82:241-243 Iyler SS, Rojas M: Anti-inflammatory effects of mesenchymal stem cells: novel ... release of mesenchymal stem cells into circulating blood Sleep 2009, 32:117-119 Javazon EH, Colter DC, Schwarz EJ, Prockop DJ: Rat marrow stromal cells are more sensitive to plating density and expand...

Ngày tải lên: 12/08/2014, 11:22

... analysis and interpretation of data, and drafting or critical revision of the manuscript or both SL contributed to acquisition of data, analysis and interpretation of data, and drafting and critical ... conception and design, acquisition of data, analysis and interpretation of data, and drafting or critical revision of the manuscript or both CK and RWK contributed to study conception and design, ... MA, Mosca JD, Moorman M, Gerson SL: Phenotypic and functional comparison of cultures of marrow-derived mesenchymal stem cells (MSCs) and stromal cells J Cell Physiol 1998, 176:57-66 Sanz-Rodriguez...

Ngày tải lên: 12/08/2014, 15:23

... CHARACTERIZATION OF THE SECRETION OF MESENCHYMAL STEM CELLS AND ITS RELEVANCE TO CARDIOPROTECTION LAI RUENN CHAI (B.Eng (Hons.)), NTU A THESIS SUBMITTED FOR THE DEGREE OF DOCTOR OF PHILOSOPHY NUS ... development of adjunctive reperfusion therapy LIST OF TABLES Supplementary Table 1.1: Proteomic profile of CM as determined by LC MS/MS and antibody array 35 Table 3.1: Proteomic profile of independently ... Mesenchymal Stem Cells In The Treatment Of Acute Myocardial Infarction With the emergence of stem cells as potential regenerative medicine, attempts to use stem cells to reduce infarct size and...

Ngày tải lên: 10/09/2015, 08:42

... CELLS 1.3.1 Definition of mesenchymal stromal cells 1.3.2 Heterogeneity of mesenchymal stromal cells 10 1.3.3 Homing of mesenchymal stromal cells 14 1.3.3.1 Mechanism of MSC homing 15 1.3.3.2 ... effects of mesenchymal stromal cells 1.4 19 PROGRESS IN IMMUNOLOGICAL STUDY OF MESENCHYMAL STROMAL CELLS 20 1.4.1 Immunogenicity of mesenchymal stem cells 22 1.4.2 Immune modulation by mesenchymal stromal ... ARE STEM CELLS 1.2.1 Definition of stem cells 1.2.2 History of stem cells 1.2.3 Common characteristics of stem cells 1.2.4 Classification of stem cells 1.3 WHAT ARE MESENCHYMAL STROMAL CELLS 1.3.1...

Ngày tải lên: 15/09/2015, 22:09

... overexpression of TPD52 and (B) cell proliferation after downregulation of TPD52 Viability of cells was measured in a colorimetric MTT assay by the detection of formazan formation The proliferation of control ... transfected cells (B) Cells transfected with specific shRNA after (C) 24 h and (D) 48 h (D) Overlay of (A) and (C) TPD52 downregulation activates caspase activity and increases the loss of mitochondrial ... CARD10 [32] and Vav3 [33], and integrins is important in determining the formation of metastatic cells [34,35] The expression of murine TPD52 in NIH3T3 cells induces the expression of several...

Ngày tải lên: 30/03/2014, 02:20

... DCTs and DCTIs have an optimal antigen uptake capacity and upregulation of CD86, CD40 and class I, but low levels of CD83, as is expected of non-terminally mature DCs The phenotypic profile of ... SCC-4 cells (PKH26 prelabelled) with 100,000 PBMCs and incubated for h Cells were stained with FITC-conjugated Annexin V and ToPro3 (Sigma Aldrich, UK) and cells that were PKH26+, annexin high and ... or is cryptic on the surface of tumour cells and that immunization of cancer patients with hTERT p540 leads to the production of T cells that not recognize tumour cells in vivo based on this epitope...

Ngày tải lên: 18/06/2014, 15:20

... at a density of × 10 cells in 0.1 mL of culture medium per well and grown in DMEM with 10% FCS in 5% CO2 at 37°C The number of cells per well was quantified by daily measurement of the absorbance ... migration and invasion assays, the cells were removed from the upper surface of the filter by scraping with a cotton swab after 12 and 24 hours in culture respectively Migrated cells and invasive cells ... of the Mia pcb6 cells, and Meng et al Journal of Translational Medicine 2010, 8:61 http://www.translational-medicine.com/content/8/1/61 Page of 14 Figure Stable overexpression and silencing of...

Ngày tải lên: 18/06/2014, 16:20

... compared with embryonic stems cells and bone marrow-derived mesenchymal stem cells, adiposederived mesenchymal stem cells (ADMSCs) have the distinct advantages of being abundant, easy to obtain ... groups Page of 16 and and between groups and (Figure 4A) However, this protein expression was substantially lower in group than in other groups and notably lower in groups and than in groups and (Figure ... Ratajczak J, Ratajczak MZ: Trafficking of normal stem cells and metastasis of cancer stem cells involve similar mechanisms: pivotal role of the SDF-1-CXCR4 axis Stem Cells 2005, 23:879-894 Kee HJ, Sohn...

Ngày tải lên: 18/06/2014, 16:20

... the level of cells that home to tumors or other sites The tumor homing behavior of MSCs was demonstrated by the mere presence of these cells in the sites of interest and/ or lack of such cells in ... unmodified cells Given complexity of the processes of biodistribution and homing of injected cells, we reasoned that an effect of engineered cell targeting would be more pronounced and better ... (211744 ± 178135 and 91023 ± 84675 cells/ mg protein), and corresponded only to 2.3 times difference The means and the standard deviation range was affected by the small number of animals and individual...

Ngày tải lên: 20/06/2014, 07:20

... FACStarplus cell sorter OC cells were gated based on forward and side scatter, and the frequencies of CD90+ and CD166+ cells were determined following a second gate on CD9+ cells In vitro chondrogenesis ... Briefly, expanded OC-derived cells and sorted OC cells were released by trypsin treatment, counted and resuspended in 15 ml polypropylene conical tubes at a density of × 105–106, and short spun ... combinations of CD9, CD44, CD54, CD90 and CD166 as positive markers, and CD45, CD133/-1 and -2 as negative markers to eliminate haematopoietic and endothelial cells (Table 2) The expression of progenitor...

Ngày tải lên: 09/08/2014, 01:23

... Induction of HLA class I and class II expression by IFN in adherent cells (a) One example of basal and IFN-γ-induced expression of HLA-A, HLA-B, cells HLA-C (left) and HLA-DR (right) molecules on mesenchymal ... standard error of the mean One representative experiment out of six different samples of bone marrow mesenchymal stem cells (three normal, two osteoarthritis and one rheumatoid arthritis) and ... significantly induced the expression of class II molecules, and both the number of positive cells (56 ± 16% and 36 ± 17% for BM-derived and synoviumderived cells, respectively) and their mean fluorescence...

Ngày tải lên: 09/08/2014, 07:20

... and isotype-switching of B cells/ B cells Ligand for CD40/T cells Critical for T and B cell antigen receptor mediated activation/ all haematopoietic cells Ligand for CD28 and CD152 (CTLA-4), T-cell ... costimulation/B cells Ligand for CD28 and CD152 (CTLA-4)/B cells Growth factor receptor/haemopoietic stem and progenitor cells, mast cells Function unknown/haematopoietic stem cells and precursors ... marrow-derived mesenchymal stem cells and neural progenitor cells Haematologica 2003, 88:126-133 Reyes M, Verfaillie CM: Characterization of multipotent adult progenitor cells, a subpopulation of mesenchymal...

Ngày tải lên: 09/08/2014, 08:23

... figures were performed with the aid of software (Origin Version 7.5 and SPSS Version 13.0) Results Expression of b1- and b2-adrenergic receptors in SGC7901 and BGC-823 cells Because propranolol is a ... antagonist, the expression of b1- and b2-ARs was determined at both the mRNA and protein level in SGC-7901 and BGC-823 cells by RT-PCR and western blot Our results showed that b1- and b2-adrenergic receptors ... improved the biological effect of irradiation and down regulated expression of the Figure Dose-survival curves of BGC-823 and SGC-7901 cells after different doses of radiation with or without propranolol...

Ngày tải lên: 09/08/2014, 09:20

... expression of these MMPs in MSCs, although the presence of MMP-2 and MMP-3 and absence of MMP-1 and MMP-9 have been reported [30] We confirmed these data and also showed the expression of MMP-28 and ... Expression levels of ADAM and adhesion molecules during chondrogenic differentiation of mesenchymal stem cells (MSCs) The gene-expression (MSCs) profile of mesenchymal stem cells (MSCs; n = 2) ... Expression of members of the integrin family The integrin family of CAMs are transmembrane glycoproteins, composed of α and β subunits and their combination determines ligand specificity The patterns of...

Ngày tải lên: 09/08/2014, 10:20

... Phenotypic profiles of HPB-AML-I The expression of MSC-related antigens in the HPB-AML-I cell line is shown (A) CD45 expression of round-polygonal HPB-AML-I cells (upper) and of the cells, which ... Regulation of stemness and stem cell niche of mesenchymal stem cells: implications in tumorigenesis and metastasis J Cell Physiol 2010, 222:268-277 Ohishi M, Schipani E: Bone marrow mesenchymal stem cells ... consisted of cells with a flattened or spindle-like morphology and that the capability of differentiating toward adipocytes of the spindle-like MSCs was superior than that of the flattened cells...

Ngày tải lên: 10/08/2014, 10:20

... ratio of MSCs to cancer cells, nature of tumour cells and cancer stem cells, integrity of immune system, number of stem cell passages and site of injection; all can affect the outcome of MSCs ... migration of mesenchymal stem cells to conditioned medium from tumor cells or bone marrow cells Stem Cells 2007, 25:520-528 Reya T, Morrison SJ, Clarke MF, et al: Stem cells, cancer, and cancer stem cells ... reaction volume of 20 μl was prepared, of which μl of starting RNA material was included for RT-PCR, a final concentration of 0.5 μM of each forward and reverse primer and 0.2 μM of the TaqMan...

Ngày tải lên: 10/08/2014, 10:21