crude fiber wendee method

Ngày tải lên: 08/08/2014, 00:22

... section 2 they will be in breach of the law and liable for a fine of $25 000. Safe carrying methods Carrying heavy objects can put a strain on your back, arms, shoulders and knees because ... that are too heavy for you to lift or carry. In these cases, you may need to use some of these methods: • Work with someone to carry the load. • Use a trolley. • Use a forklift or trolley jack...

Ngày tải lên: 21/08/2012, 17:56

... 140." and explains the impact of this access method for the problem of the hided terminal and of the exposed terminal. MACA - A New Channel Access Method for Packet Radio Phil Karn [ ] Turning ... be able detect the lost frames. 1. Pure Aloha In order to determine performances of this access method of the simple Aloha protocol, one will suppose that the frames are of length fixed t and ... - MOB Mobile Internet and Surrounding 1/12 Baey, Fladenmuller – Subject 5 MOB Subject 5 –Access methods Static and dynamic access techniques 1. Static access techniques 1.1 Space-division multiple-access...

Ngày tải lên: 17/09/2012, 09:13

... Biochem. Mol. Biol. 27, 5792. Methods in Molecular Biology TM HUMANA PRESS Glycoprotein Methods and Protocols Edited by Anthony P. Corfield VOLUME 125 The Mucins Methods in Molecular Biology TM HUMANA ... and lectins or coating methods such as the glycan detection method and enzyme-linked immunosorbent assays (ELISA). All these techniques have advantages and disadvan- tages. Solution methods often crave ... This method allows the group separation of large amounts of mucins from nucleic acids and proteins/lipids under dissociative conditions without the problems associated with matrix-based methods such...

Ngày tải lên: 23/09/2012, 19:25

... nonsulfated mucins remain unstained. Histological Methods for Detection of Mucin 29 29 From: Methods in Molecular Biology, Vol. 125: Glycoprotein Methods and Protocols: The Mucins Edited by: A. ... tract. Histological Methods for Detection of Mucin 39 3. Alternatively, prepared adhesive slides such as Superfrost Plus ® /(Menzel-Gläser, Braunschweig, Germany) may be purchased. 4. There are many other methods ... J. A. (1990) Histological and Histochemical Methods: Theory and Practice, Pergamon, Oxford, UK. 46. Andrew, S. M. and Jasani, B. (1987) An improved method for the inhibition of endo- genous peroxidase...

Ngày tải lên: 23/09/2012, 19:25

... Mucins can be detected by chemical methods within acrylamide Detection and Quantitation of Mucins 45 45 From: Methods in Molecular Biology, Vol. 125: Glycoprotein Methods and Protocols: The Mucins Edited ... mucins, in Glycoprotein Methods and Protocols: The Mucins (Corfield, T., ed.), Humana, Totowa, NJ. 3. Carraway, K. L. (2000) Preparation of membrane mucin, in Glycoprotein Methods and Protocols: ... (6). References 1. Walsh, M. D., Jass, J. R. (2000) Histologically-based methods for detection of mucin, in Glycoprotein Methods and Protocols: The Mucins (Corfield, T., ed.), Humana, Totowa, NJ. 2....

Ngày tải lên: 23/09/2012, 19:25

... that the dye penetrates the mucous layer uniformly. Results from this method are therefore difficult to interpret and this method is not considered fur- ther here. The best approach to quantitating ... sections of unfixed mucosa (detailed subsequently) has been a simple method for measuring mucous thickness, although histological methodology has now reached the stage where preservation of full ... This method, which for experimental practicali- ties can be applied only to the rat (and possibly other experimental animal models), is particularly useful for validating other in vitro methods...

Ngày tải lên: 23/09/2012, 19:25

... PhosphorImager as au/A 260nm . Biosynthesis and Secretion of Mucin 65 65 From: Methods in Molecular Biology, Vol. 125: Glycoprotein Methods and Protocols: The Mucins Edited by: A. Corfield © Humana Press ... is isolated according to the manufacturer. Measure the following components: 68 Dekker et al. 3. Methods 3.1. General Assays for Quantitation of Mucin ( see Notes 1–3) Each of these six detailed ... au/milliliter of homoge- nate or au/milliliter of medium (see Note 5). 6. Isolate RNA by the Trizol method, and quantify RNA at A 260nm /milliliter. Judge the intact- ness of the RNA, by analysis...

Ngày tải lên: 23/09/2012, 19:25

... there are few separation techniques available to use with these 77 From: Methods in Molecular Biology, Vol. 125: Glycoprotein Methods and Protocols: The Mucins Edited by: A. Corfield © Humana Press ... biochemical separation methods. We describe a separation protocol based on anion exchange chro- matography to fractionate the different species of mucin coupled with agarose gel electrophoresis as a method to ... Biotechnol. 5, 171–176. 10. Thornton, D. J., Howard, M., Devine, P. L. and Sheehan, J. K. (1995) Methods for separa- tion and deglycosylation of mucin subunits. Anal. Biochem. 227, 162–167. Separation...

Ngày tải lên: 23/09/2012, 19:26

... spreading method was originally developed by Kleinschmidt (10a) using cyto- chrome C as the spreading agent and was subsequently developed by others (11). We describe here a modified method first ... of the method is the creation of a monolayer in which the long filamentous molecules are gently entrapped and thereafter can be removed onto the Fig. 3. Schematic diagram of spreading method. ... 5). Heterogeneity and Size Distribution of Gel-Forming Mucins 87 87 From: Methods in Molecular Biology, Vol. 125: Glycoprotein Methods and Protocols: The Mucins Edited by: A. Corfield © Humana Press...

Ngày tải lên: 23/09/2012, 19:26

... code as defined in Fig. 1. Amino Acid Analysis of Mucins 113 113 From: Methods in Molecular Biology, Vol. 125: Glycoprotein Methods and Protocols: The Mucins Edited by: A. Corfield © Humana Press ... does not take into account the amount of cysteine and tryptophan. We are currently developing a method for quantitating cysteine by amino acid analysis. Table 2 Example of Quantitative Calculation ... C18 reversed-phase column and detected by fluorescence. Our routine analyses have shown that the method described here is reliable and useful for both quantitative and qualitative mucin glycoprotein...

Ngày tải lên: 23/09/2012, 19:26

... Sequencing Glycopeptides 121 121 From: Methods in Molecular Biology, Vol. 125: Glycoprotein Methods and Protocols: The Mucins Edited by: A. Corfield © Humana Press ... Elsewhere we have published alternative HPLC methods for the separation of PTH-glycosylated amino acids on a modified Beckman LF3600 sequenator (12). These methods were developed for two reasons: ... glycosylated amino acids are only recovered in low yield. Samples are sequenced (Fig. 1) on glass- fiber supports or membranes such as polyvinylidene difluoride (PVDF). Following cleavage, the released...

Ngày tải lên: 23/09/2012, 19:26

... interrogation of antibody specificity; however, the methodology for their preparation is beyond the scope of this chapter. By far the most widely used method for modifying short peptides so that they ... R Trityl C Synthetic Peptides for Antimucin Antibodies 129 129 From: Methods in Molecular Biology, Vol. 125: Glycoprotein Methods and Protocols: The Mucins Edited by: A. Corfield © Humana Press ... cannot be controlled or simply that short peptides do not adhere well to polystyrene plates. Several methods of peptide modification have been utilized to overcome these problems. One such procedure...

Ngày tải lên: 23/09/2012, 19:26

... replaced with an 88-kDa band Dimerization of Domains of Mucin 143 143 From: Methods in Molecular Biology, Vol. 125: Glycoprotein Methods and Protocols: The Mucins Edited by: A. Corfield © Humana Press ... 0.5% Nonidet P-40. 9. Neuraminidase incubation buffer: 40 mM Tris-HCl, 4 mM CaCl 2 , pH 7.8. 3. Methods 3.1. Synthesis of Rat Muc2 Domain Construct (pRMC) Using Recombinant PCR Figure 1 shows ... C-terminal domain of rat intestinal Muc2 is capable of dimerization through its cysteine residues. This method avoids, to a large extent, the numerous difficulties of dealing with full-length mucins,...

Ngày tải lên: 23/09/2012, 19:26

... (Kdn), respectively. Substituents of the hydroxyl groups present on From: Methods in Molecular Biology, Vol. 125: Glycoprotein Methods and Protocols: The Mucins Edited by: A. Corfield © Humana Press ... of the substituents, largely O-acetyl groups are quite labile during acid or alkaline hydrolysis methods generally utilized for the release of monosaccharides. Consequently, the study of sialic ... sialic acid modifi- cations has been attempted after release and purification by improving the methods to avoid any destruction, and is achieved either with low concentrated acid solutions (3) or...

Ngày tải lên: 23/09/2012, 19:26

... of chromatographic methods. J. Immunol. Methods 213, 113–130. 16. Guile, G. R., Rudd, P. M., Wing, D. R., Prime, S. B., and Dwek, R. A. (1996) A rapid high resolution HPLC method for separating ... GalNAc-Ser/Thr, often found in mucins, is destroyed by this procedure. From: Methods in Molecular Biology, Vol. 125: Glycoprotein Methods and Protocols: The Mucins Edited by: A. Corfield © Humana Press ... results in a new aldehyde being formed for reductive amination, and, indeed, this is the basis for a method for structural analysis by thin- layer chromatography-mass spectometry (MS) (4). Note, however,...

Ngày tải lên: 23/09/2012, 19:26

Ngày tải lên: 23/09/2012, 19:26

Ngày tải lên: 23/09/2012, 19:26

Ngày tải lên: 23/09/2012, 19:26

Ngày tải lên: 23/09/2012, 19:26