cronbach s alpha for inconsistent responses

... This result was consistent with previous published results, using the cat reporter system (pDM138), this construct has a design similar to the unspliced luc+ reporter construct used in this study ... expression of HBV transcripts [47,48] As this siRNA target site (HBV PRE 1317-1337) is present in all HBV transcripts, it is possible that any or all HBV transcripts were reduced by the siRNA, resulting ... of these cell lysates were assayed for expression of luciferase proteins using POLARstar OPTIMA (BMG Labtech) Each assay was repeated in triplicate The mean values and standard deviations of three...

Ngày tải lên: 12/08/2014, 01:21

... No No No No No No No No No Yes Yes Yes Yes Yes Yes Yes Yes Yes Yes Yes Yes Yes Yes Yes No Yes Blast results Consistency Consistency Consistency Inconsistency Consistency AK027322 Unmatched Unmatched ... all tags tested using the standard PCR condition (first PCR, 94 °C for 30 s, 55 °C for 30 s and 72 °C for 30 s for 15 cycles; second PCR, 94 °C for 30 s, 60 °C for 30 s and 72 °C for 30 s for 25 ... serial analysis of gene expression tags for gene identification Proc Natl Acad Sci USA 97, 349–353 Richards M, Tan SP, Chan WK & Bongso A (2006) Reverse serial analysis of gene expression (SAGE) characterization...

Ngày tải lên: 07/03/2014, 04:20

... conditions: 50°C for minutes, 95°C for minute, 45 cycles of 95°C for 15 seconds 55°C for 30 seconds and 72°C for 30 seconds Data analysis The ΔΔCT [23] method was used for initial data analysis Four ... experimental settings Additionally BACT and HMBS also had high sumv values of 10.51 and 9.65 respectively, indicating that these genes are generally less reliable for use as normalisers for QPCR assays ... 4:130 strain of virus and the cell type infected Thus it is not possible to identify a single housekeeping gene for use in QPCR studies of viral infections Nevertheless it is of benefit for researchers...

Ngày tải lên: 18/06/2014, 18:20

... conditions: 50°C for minutes, 95°C for minute, 45 cycles of 95°C for 15 seconds 55°C for 30 seconds and 72°C for 30 seconds Data analysis The ΔΔCT [23] method was used for initial data analysis Four ... experimental settings Additionally BACT and HMBS also had high sumv values of 10.51 and 9.65 respectively, indicating that these genes are generally less reliable for use as normalisers for QPCR assays ... 4:130 strain of virus and the cell type infected Thus it is not possible to identify a single housekeeping gene for use in QPCR studies of viral infections Nevertheless it is of benefit for researchers...

Ngày tải lên: 20/06/2014, 01:20

... positive HBeAg and for 58% with positive anti-HBe sera Table shows the HBe Ag status and the PCR amplification results in the studied population PCR-RFLP and TSP-PCR were successfully assessed ... automated sequencer (Applied Biosystems, Darmstadt, Germany), with the same primers as those used for PCR amplification of pre -S region The sequences obtained were compared with published sequences from ... 160 pb The presence of the same site of restriction in this region of genotype D strains was also observed in sequences available in GenBank under the following assession numbers: DQ464170, EU594406,...

Ngày tải lên: 12/08/2014, 02:20

... volumes of ice cold RBC lysis buffer (0.17M NH4Cl) was added and kept on ice for complete lysis of RBCs The leucocytes were spun down at 4000 rpm for 15 and the supernatant containing lysed RBCs ... Murrah buffaloes RESULTS AND DISCUSSION Primers used for cattle (Meignanalakshmi et al., 2001) were found to be suitable for amplifying b-lg gene in Murrah buffaloes ,which resulted in 262 bp ... was digested with 20 units of EcoRI, HindIII, PstI and Hae III at 370C for h The restriction fragments were resolved by electrophoresis in 4% Agarose gel in 1X TBE buffer 100 bp ladder was used...

Ngày tải lên: 18/02/2014, 02:20

... Sugano S & Matsushima K (2004) 5¢-End SAGE for the analysis of transcriptional start sites Nat Biotechnol 22, 1146– 1149 Kasai Y, Hashimoto S, Yamada T, Sese J, Sugano S, Matsushima K & Morishita S ... primer for synthesizing first-strand cDNA to serve as PCR template For PCR, the tag-specific primer was used as the sense primer, and consisted of the 14-base tag nucleotides and 5¢ inosine nucleotides ... many reagents are shared by these two protocols Figure shows the detailed process (see protocol in Scheme in the Appendix for more details) As shown in Fig 4, steps 1–5 are shared by the SAGE protocol,...

Ngày tải lên: 07/03/2014, 00:20

... 60CS 51 ,9.1 5P 90HS 12 ,02 ,51 4P 90CS ,80CS ,21HS ,80HS 12 ,91 ,51 3P 50HS 32 ,51 2P 40CS ,40CS ,20CS ,10CS ,11HS ,01HS ,70HS ,60HS ,40HS ,30HS ,20HS ,10HS 12 ,51 1P sniartS )bk( sezis dimsalP ... 90HS 3P namuH EP 80HS 1P namuH EP 70HS 1P namuH 60HS 2P namuH EP 50HS 1P namuH 40HS 1P namuH 30HS 1P namuH EP 20HS 1P namuH EP 10HS seliforp *snrettap secruoS niartS dimsalP ecnatsiseR setalosi ... fo sniarts gnoma spihsnoitaler citeneg ssessa ot sremirp XOB dna CIRE gnisu sdohtem RCP-per yb deziretcarahc erew nekcihc dna namuh morf setalosi siditiretnE S 22 ,yduts siht nI seicepsbus allenomlaS...

Ngày tải lên: 07/08/2014, 18:21

... in the tissue, biopsies were collected from multiple sites of individual synovial tissue specimens Tissue samples were similar in size to those obtained by percutaneous blind-needle biopsy (1–2 ... using about six tissue samples allows for the detection of twofold differences in gene expression Interestingly, this number is similar to previous observations derived from immunohistochemistry ... Validation studies performed on surgical samples suggest that the techniques, especially in combination with protein analysis techniques such as IHC, can be applied to serial biopsy studies using to...

Ngày tải lên: 09/08/2014, 01:23

... involved in SAPHO (synovitis, acne, pustulosis, hyperostosis, and osteitis) syndrome, which is an oligoarthritis associated with acnes and pustilosis [37,38] – was detected in ReA and UA samples Detection ... patients [6] Most Shigella ReA cases are caused by S flexneri [35], but sporadic cases associated with S sonnei and S dysenteriae have been described [4,35] The most recent published case of S sonnei ... articular samples from patients with SAPHO (synovitis, acne, pustulosis, hyperostosis, and osteitis) syndrome, which is often regarded to be a form of spondyloarthropathy, suggesting an infectious origin...

Ngày tải lên: 09/08/2014, 10:23

... 1.63% Besides, the expression of the gene encoding methionineadenosyl transferase (S- adenosyl-L-methionine synthetase, SAMS), the enzyme responsible for the synthesis of S- adenosylmethione (SAM) ... Clhomeostasis and osmotic adjustment in plants under NaCl stress [80] The overexpression of the genes encoding various transcription factors under NaCl stress in S maritima is no doubt suggestive ... Trends Plant Sci 2007, 12:419-426 Henriksson E, Olsson AS, Johannesson H, Johansson H, Hanson J, Engstrom P, Soderman E: Homeodomain leucine zipper class I genes in Arabidopsis Expression patterns...

Ngày tải lên: 12/08/2014, 03:20

... also estimated Statistical analysis Pearson s correlation coefficient was used to assess the strength of the linear association between the log 10 transformed values of the estimated (copies/mL) ... quantifications by using the current and the previous in-house assay was also estimated Comparison of the ultra sensitive RTQ-PCR vs COBAS TaqMan HBV Test To compare the ultra sensitive RTQ-PCR assay with ... regression, a conversion formula was calculated for the in-house measurements (copies/mL) to the international standard units (IU/mL) Comparison of the ultra sensitive RTQ-PCR vs the previous real-time...

Ngày tải lên: 12/08/2014, 04:20

... has revealed a preference for transcription start sites, CpG islands, DNase-hypersensitive sites and gene-dense regions as is typical for an MLV Although primary DNA sequence is not regarded as ... of this integration site (EU981810) from patient tissue [7] Discussion Current knowledge based on the analysis of several thousand retroviral integration sites suggests that target site selection ... units whereas MLV tends to integrate near transcription start sites and CpG islands Both have a preference for gene dense regions Analysis of several hundred XMRV integration site sequences [8,9]...

Ngày tải lên: 13/08/2014, 01:20

... S1 S2 S3 S4 S5 S6 S7 S8 S9 S1 0 S1 1 NTC S1 REF1 S2 comment REF1 Volume 8, Issue 2, Article R19 S3 REF2 S4 S5 REF3 S6 reviews S7 GOI1 NTC REF1 REF2 REF3 GOI1 GOI2 GOI3 S1 S2 S3 S4 S5 S6 S7 S8 S9 ... S1 0 S1 1 NTC S1 GOI2 S2 S3 GOI3 S9 S1 0 reports S8 S1 1 NTC REF1 REF2 REF3 GOI1 GOI2 GOI3 deposited research S1 ’ S2 ’ S3 ’ S8 S9 S1 0 S1 1 Figure Experimental setup Experimental setup Experimental setup ... qBase is especially useful and unique for analysis of experiments containing multiple runs As users are usually interested in comparing the expression for a given gene between different samples,...

Ngày tải lên: 14/08/2014, 17:22

... “Additional Constraint“ for superposition files Correction of join results in “DoList” for load cases Additional options in PLSYS for representing of stresses (FIBSUP) Minor improvements in lists and ... Constraints modified Composite cross section modified ( “Properties/CS” ) Cross section shear stresses calculation added (graphical presentation “Properties/CS” and stage action “PlShear”) New LoadSet ... number of time steps ) Extensions of British Standard live load calculation Added possibility to force TCL-export with cross section values even if cross sections are assigned 3.28 Version 8.50.01...

Ngày tải lên: 06/09/2012, 15:18

... Properties\material\I\Fibre Stress check) Out1: -blank Out2: File name for printout Fibre Stress Plot Select Results\PlSys to specify the fibre stress plots: Basic definition Select Results\PlSys\Macro\Load ... identify the Stress Points (say STRESS) Point Insert Specify Stress Points Recalculate RM RM2000: Call for Fibre Stress calculation in the Construction Schedule Construction Schedule / Stage Calculation ... Construction Schedule Loads LCase Define the stressing actions Define a prestressing loading case & assign the load set to it Construction Schedule Stage Tendon PREL Calculating the prestressing...

Ngày tải lên: 06/09/2012, 15:55

... 3.2.6 Stress limits for (pre-stressed concrete) fibre stress checks Concrete stress limits for different load combinations as described in 3.2.6 Material properties for principal stress check (shear ... generally used in the theory of elasticity (based on a right-handed system!) These conventions define tensile stresses being positive and compression stresses being negative Shear stresses are positive, ... • Cross-section lengths used as input values for the cross section definition macros, such as width, height, thickness of cross-section components • Coordinates of the nodes of the cross-section...

Ngày tải lên: 06/09/2012, 15:55

... hazards assumption was validated for each model Statistical Analysis Software version 9.1.3 (SAS Institute, Inc., Cary, NC, USA) was used for statistical analyses P values less than 0.05 were considered ... shorter for doripenem; betweengroup differences in hospital stay were not statistically significant Discussion The results of this pooled analysis of two phase III studies indicated that initial use ... concentration; MRSA: methicillinresistant Staphylococcus aureus; VAP: ventilator-associated pneumonia Competing interests This study, including statistical analysis and manuscript preparation, was supported...

Ngày tải lên: 25/10/2012, 10:02

... Peak systolic tissue velocity [cm /s] ; SR: strain rate [s- 1]; sSR: peak systolic strain rate [s- 1]; SRe: eak early diastolic systolic strain rate [s- 1]; ROI: Region of interest; TDI: Tissue Doppler ... longitudinal deformation were: 2DS -11.67 ± 5.38 %; systolic SR (sSR) -0.65 ± 0.27 s- 1; early diastolic SR (Sre) 0.60 ± 0.35 s- 1 The analysis of tissue velocities demonstrated a gradient of systolic (S ) ... according to 2DS, dSR and tissue velocities, which is depicted in Table and Figures 2, Infarcted segments demonstrated significantly decreased 2DS as well as tissue velocities in comparison to adjacent...

Ngày tải lên: 25/10/2012, 11:15

... antiretroviral drugs There are two types of stress: distress (the negative type), and eustress (the positive type also known as mirthful laughter) Distress was shown to increase stress hormones such as beta-endorphins, ... consists of two elements: the conscious and unconscious The conscious mind explicitly assesses situations, whereas the unconscious mind remains implicit (29) Thus, this question expected a conscious ... different sets of responses were encountered Firstly, responses to both questions corresponded to the same level of laughter, and thus, it was accepted Secondly, for instance if a response belonged...

Ngày tải lên: 26/10/2012, 09:57