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Ngày tải lên: 10/08/2014, 09:21

... of the D278 mutant indicates the partial contribution of this side chain to the signals of the protonated acidic residues for the oxidized form in the absence of the inhibitor. The shifts of the ... respectively, from the Q o binding site (see Fig. 1), as measured from the chromone head- group of the inhibitor [2]. Their involvement in a water chain leading up to the heme b L edge, and their parti- cipation ... observed for the Y302F and the Y297F mutant enzymes. These shifts are rather small as com- pared to previously published absorption coefficients for these modes [23,25,28,29]. In the spectra of the Y302F...

Ngày tải lên: 23/03/2014, 10:20

... proteins, including other GHs (clans E, F and J) [3] and hemo- pexin [20]. The CD spectrum of the latter protein [21] is very similar to the one observed here; therefore, the unusual spectrum ... Avicel were obtained from Sigma- Aldrich, and the cello-oligosaccharides were obtained from Merck (Darmstadt, Germany). The oligosaccharides derived from b-glucan were a gift from A Planas (Universi- tat ... incubation with Cel74A, and the proposed cleavage pattern (C) with the theoretical m ⁄ z value (z in superscript) of the most important fragments (the position of the Gal residues is arbitrary)...

Ngày tải lên: 19/02/2014, 05:20

... ODYSSEUS TO THE MAST the product itself, rather than from simply being offered the product. These effects support the point estimates discussed above. In order to calculate the Treatment on the Treated ... effects estimated in the pre- vious specifications, and suggests that the impact of the commit- ment product came from the product itself, and not from the door-to-door marketing. Further supporting ... period. Comparing the treatment group to the control group, the largest treatment effects—in both the six-month and one-year periods—are for the very bottom of the distribution, the lowest decile, and for the...

Ngày tải lên: 06/03/2014, 10:20

... stems from t he P 3+ cluster, then the g ẳ 2.27 feature derives from the FeFe c ofactor. On the other hand, if the broad signal, d espite its unusual properties, represents the P 1+ cluster, the ... absent. The profile of the entire signal, without interference of the rhombic s ignal, was obtained by subtracting the pH 6.4- spectrum from the pH 8.4-spectrum (see the inset of Fig. 3). The axial ... of these novel EPR signals to either the cofactor or the P-cluster has proven elusive due to the fact that both of these metal clusters present in the Fe-only n itrogenase are d iamagnetic in the...

Ngày tải lên: 18/03/2014, 01:20

... saturat- ing) and the variation of initial rate with the concen- tration of the second substrate is then used to estimate k cat and the K m for the variable substrate. However, the assumption that ... bound at the active site. The Y102A mutant therefore accomodates a variant config- uration of biliverdin to the wild-type enzyme but retains the ability to catalyse the transfer of hydride from both ... and varying the concentration of NADPH from 5–100 lm or NADH from 50–1000 lm. The ‘fixed’ concentra- tions of biliverdin were also varied from 0.5–10 lm to yield the plot for NADPH as the variable...

Ngày tải lên: 23/03/2014, 05:22

... changes in the HSQC spectrum of the cÂe complex by mutating the cE204 residue to Gln. Inset in (B) indicates the changes in the selected areas of the HSQC spectrum of the cÂe complex imposed by the ... with the catalytic sites for ATP synthesis, and the membrane-embedded F o domain (bacterial subunits ab 2 c 10–15 ), with the sites for the translocation of the ions. In ATP synthesis mode, the F o motor ... empty chip), allowing the elimination of the latter by baseline correction (see below). As a result of the location of the His tag on the very top of the c sub- unit, the immobilized cÂe complexes...

Ngày tải lên: 24/03/2014, 00:21

Ngày tải lên: 11/06/2014, 09:44

Ngày tải lên: 13/08/2014, 13:20

Ngày tải lên: 13/11/2014, 15:42

Ngày tải lên: 26/03/2015, 10:47

... in the protein stability of our mutants. There- fore, these conclusions strengthen our hypothesis that the catalytic efficiencies of the recombinant enzymes used in our study were related to the ... transfer from the two-electron substrate NADPH to Fd pro- ceeds in an ordered pathway, in which the observed K m value results from the sum of the K m values for the successive interaction of the ... by DNA sequencing. The design of the mutants was intended to preserve the amino acid character and to modify only the relative volume of their R groups. The expression of the FNR mutants as soluble...

Ngày tải lên: 18/02/2014, 17:20

... private rather than public terms, relating them to the play of the imagination rather than the exercise of reason. But these shifts cannot erase the important continuities that existed between the ... elites it was intent on displacing on the one hand and from the various popular and plebeian strata it aspired to rule on the other. 19 The first of these shifts, from a focus on a cultural product (literature) ... prefaced with a statement of the editor’s commitment to the Enlightenment30 Literature has reconciled the whole thinking world respecting the great principles of the system of the universe, and extirpated...

Ngày tải lên: 21/09/2012, 11:00

... Figure 5C shows that the spectra of the sample subjected to compression and decompression are similar to the non-treated sample, even in the case of the M88V mutant. The elution of the samples after ... as the major virus structural protein, forming the shell in which the RNA genome is contained [11,12]. The tertiary structure and topology of the MS2 coat protein is different from those of other ... interacts with residues on the adjacent b-strand (F) and with amino acids in the alpha-helical regions as they pass over the b-sheet. Thus, the M88V mutant substitution affects the dimer interface,...

Ngày tải lên: 19/02/2014, 12:20

... prisoners, the nature of their offenses or the lengths of their sentences in a timely manner. Since the demographics and criminal history of these offenders are unknown, they cannot be factored into the ... in most other jurisdictions, the growth of the corrections system has had a profound effect on the state’s fiscal resources. The cost of the Oklahoma corrections system has tripled over the last ... open access to their facilities over the course of this project. Without the assistance of the many line staff and managers who took time from there assigned duties to explain their operations...

Ngày tải lên: 16/03/2014, 00:20

... children, but they should also consider adults, as their competence to make healthy choices or their capacity to resist the marketing of unhealthy food may not fully protect them from the damage ... things. The dynamics of the food system, particularly in the expanding markets of eastern Europe, needs further ex- ploration and the evolution of the food supply, particularly the price and availability ... increased over the past decade (Fig. 10). 4. The European Region has the highest alcohol consumption in the world, particularly among men (69). Wine is generally preferred in southern Europe,...

Ngày tải lên: 16/03/2014, 14:20

... exactly the Egypt of ancient days is described in the Bible! The dress the people wore, the food they ate, the way they spoke to their kings, the description of their funerals, the very name of their ... through the walls of the Holy City. His ears rang with the cries of rage and despair which broke from the Jews within, as one by one their defences fell, and the end drew near! Then food failed in the ... BEHIND THE CLERK'S EAR] So the questioners settled the matter in their own minds; but God had the answer to their questions all ready for them. He put into the hearts of some brave men the...

Ngày tải lên: 16/03/2014, 15:20

... properties of the enzyme [19]. To extend the information about the role of the noncatalytic SBD regions and their effect on the C-terminal CD, we further explored the amino acids of the N-terminal region ... Whereas the production of ADPGlc via ADPGlc PPase is the first committed step in starch biosynthe- sis, SS catalyzes the elongation of a-1,4-glucans by the transfer of the glucosyl moiety from the ... confirmed the results obtained in the pull-down assays, showing that there is a physical interaction between the N- and C-terminal domains of SSIII in vitro. Mapping of the CD-binding region in the N-terminal...

Ngày tải lên: 22/03/2014, 21:20

... Consequently, the top two carbon atoms of s7p synthesized in the half- reaction model can originate from either f6p or p5p, while in the traditional model they can only originate from p5p. In ... procedures Metabolic network model Apart from the variations in the stoichiometric model of the PPP discussed in this work, the other parts of the stoi- chiometric model used for fitting the fluxes of S. cerevisiae were ... the metabolites formed from these) can differ from the 13 C labeling of the single C 2 and C 3 fragment pools gener- ated by the half-reactions. Genetic organization of the nonoxidative branch of the PPP In...

Ngày tải lên: 23/03/2014, 15:21