coupling intracellular signaling to chromatin remodeling
Báo cáo khoa học: Activator-binding domains of the SWI ⁄ SNF chromatin remodeling complex characterized in vitro are required for its recruitment to promoters in vivo pot
Ngày tải lên :
07/03/2014, 00:20
... glucocorticoid receptor during chromatin remodeling Mol Cell 14, 163–174 Krebs JE, Fry CJ, Samuels ML & Peterson CL (2000) Global role for chromatin remodeling enzymes in mitotic gene expression ... by the same activator We chose to study the GAL regulon, which is known to be regulated by the Gal4 activator protein We first tested a group of known Gal4 target genes [38] to identify a group ... similarly to its recruitment to GAL1-10 and GAL2 The activator-binding domains of Swi1 and Snf5 are required for galactose-induced expression of SWI ⁄ SNF-dependent genes It was next necessary to determine...
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Tài liệu Báo cáo Y học: The binding of lamin B receptor to chromatin is regulated by phosphorylation in the RS region ppt
Ngày tải lên :
22/02/2014, 04:20
... NM region binds to a protein(s) on chromatin These results also suggested that the binding of the RS region to chromatin DNA is superior to the binding of the NM region to the chromatin protein ... synthetic phase cytosol, which stimulated the binding to chromatin Suppression of the binding of LBR to chromatin by phosphorylation with a kinase(s) in a mitotic phase egg cytosol Fig Stimulation ... Identification of chromatin binding regions of LBR To analyze the binding of LBR to chromatin, we used the N-terminal portion of LBR, because this portion is responsible for the binding to chromatin...
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Genes and Autoimmunity - Intracellular Signaling and Microbiome Contribution by Spaska Angelova Stanilova pot
Ngày tải lên :
17/03/2014, 21:20
... cytokines, several genes encoding different inflammatory cytokines have been studied in HT, and some have shown the ability to influence the severity of disease As HT is thought to be a cytotoxic ... Edmiston, 1999] 35 36 Genes and Autoimmunity - Intracellular Signaling and Microbiome Contribution There are strong evidences to suggest the great importance of this cytokine in the control of autoimmunity ... Thyroglobulin 13 14 Genes and Autoimmunity - Intracellular Signaling and Microbiome Contribution Categories GD including GO GO HT Cytokines or TNF-α TNF-α Cytokine receptors IL-1α IL-4 IL-1β TGF-β...
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Báo cáo khoa học: Saporin and ricin A chain follow different intracellular routes to enter the cytosol of intoxicated cells pptx
Ngày tải lên :
23/03/2014, 15:21
... Mannheim, Germany) Cytotoxicity experiments Vero cells were used to compare the cytotoxic activities of SAPwt and SAPKDEL to ricin A chain (RTAwt) or to RTAKDEL or to the ricin holotoxin and DT, used ... cytotoxicity (data not shown) The low cytotoxic activity of saporin in HeLa cells, with ID50 in the micromolar range after h of exposure (Table 1), prompted us to verify that toxicity was due to ... and HeLa cells were treated with drugs known to interfere with ricin holotoxin intracellular delivery and thus cytotoxic activity The fungal inhibitor Brefeldin A (BFA) causes Golgi complex disassembly,...
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GENES AND AUTOIMMUNITY - INTRACELLULAR SIGNALING AND MICROBIOME CONTRIBUTION docx
Ngày tải lên :
30/03/2014, 09:20
... cytokines, several genes encoding different inflammatory cytokines have been studied in HT, and some have shown the ability to influence the severity of disease As HT is thought to be a cytotoxic ... Edmiston, 1999] 35 36 Genes and Autoimmunity - Intracellular Signaling and Microbiome Contribution There are strong evidences to suggest the great importance of this cytokine in the control of autoimmunity ... Thyroglobulin 13 14 Genes and Autoimmunity - Intracellular Signaling and Microbiome Contribution Categories GD including GO GO HT Cytokines or TNF-α TNF-α Cytokine receptors IL-1α IL-4 IL-1β TGF-β...
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Báo cáo Y học: Chromatin remodeling in nuclear cloning pptx
Ngày tải lên :
31/03/2014, 21:21
... diagrammatic examples of chromatin remodeling in Xenopus egg cytoplasm (A) Nucleoplasmin exchanges somatic linker histones H1 and H1° with egg linker B4 leading to less stable chromatin that is more ... 2002 Chromatin remodeling in nuclear cloning (Eur J Biochem 269) 2285 N N A H1 B4 Stable chromatin Nucleoplasmin N N H1 B4 Less stable chromatin N B N TBP ISWI complex N N More relaxed chromatin ... exchange events give rise to more drastic and easily detectable consequences leading to dedifferentiation HISTONE ACETYLATION AND DNA METHYLATION Importantly, these chromatin remodeling events can...
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Báo cáo Y học: Nucleotide excision repair and chromatin remodeling pptx
Ngày tải lên :
31/03/2014, 21:21
... NER factors are required to overcome the structural barriers that chromatin poses to the removal of DNA damage in vivo Although histone acetylation is generally related to chromatin accessibility, ... of nucleosomes, which might be mediated by chromatin assembly factor (CAF-1) and/or chromatin remodeling complexes, incorporates the repair patch into chromatin DNA ligase I efficiently ligates the ... in chromatin [27,28] (see below) In addition, organization of DNA in chromatin affects how UV light and chemical agents impart damage to DNA In order to understand the relationship between chromatin...
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chromatin and chromatin remodeling enzymes, part b
Ngày tải lên :
11/04/2014, 01:17
... histones and chromatin regulators, methods for the preparation and analysis of histone modifying and ATP-dependent chromatin remodeling enzymes, and assays for transcription and DNA repair on chromatin ... Sequence Chromatin and Chromatin Remodeling Complexes By Rachel A Horowitz-Scherer and Christopher L Woodcock Recent progress in preparing and purifying defined sequence chromatin and chromatin remodeling ... Sequence Chromatin and Chromatin Remodeling Complexes By Rachel A Horowitz-Scherer and Christopher L Woodcock Recent progress in preparing and purifying defined sequence chromatin and chromatin remodeling...
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chromatin and chromatin remodeling enzymes, part c
Ngày tải lên :
11/04/2014, 01:19
... xvi preface histones, histone variants, modified histones and defined chromatin segments, protocols for nucleosome reconstitution and analysis, and cytological methods for imaging chromatin functions ... and chromatin proteins Volume 377 includes genetic assays of histones and chromatin regulators, methods for the preparation and analysis of histone modifying and ATP-dependent chromatin remodeling ... protocols for visualizing chromatin and detecting chromatin interactions, enzymological assays for histone modifying enzymes, and immunochemical protocols for the in situ detection of histone...
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chromatin and chromatin remodeling enzymes, part a
Ngày tải lên :
11/04/2014, 02:02
... xvi preface histones, histone variants, modified histones and defined chromatin segments, protocols for nucleosome reconstitution and analysis, and cytological methods for imaging chromatin functions ... and chromatin proteins Volume 377 includes genetic assays of histones and chromatin regulators, methods for the preparation and analysis of histone modifying and ATP-dependent chromatin remodeling ... histone octamers from other species, or containing histone variants, can be refolded to functional histone octamers according to a previously described protocol.1 The method works best for to...
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Báo cáo sinh học: " Progressive loss of CD3 expression after HTLV-I infection results from chromatin remodeling affecting all the CD3 genes and persists despite early viral genes silencing" pot
Ngày tải lên :
18/06/2014, 18:20
... Transcription factor accessibility to the CD3γ promoter after HTLV-I infection Figure Transcription factor accessibility to the CD3γ promoter after HTLV-I infection A,In vitro binding to the Spγ1/CD3γInr ... blot protocol The genomic DNA was digested with EcoRI (no cut into the HTLV-I provirus) or SacI (cut once into the HTLV-I LTR) and electrophoresed in an agarose gel then transferred to nylon ... infected cell surface, corresponding to the CD3low phenotype, was detected at to 10 weeks p.i The cells remained CD3low until receptor negative cells began to emerge around months p.i followed...
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Báo cáo hóa học: " Progressive loss of CD3 expression after HTLV-I infection results from chromatin remodeling affecting all the CD3 genes and persists despite early viral genes silencing" docx
Ngày tải lên :
20/06/2014, 01:20
... Transcription factor accessibility to the CD3γ promoter after HTLV-I infection Figure Transcription factor accessibility to the CD3γ promoter after HTLV-I infection A,In vitro binding to the Spγ1/CD3γInr ... blot protocol The genomic DNA was digested with EcoRI (no cut into the HTLV-I provirus) or SacI (cut once into the HTLV-I LTR) and electrophoresed in an agarose gel then transferred to nylon ... infected cell surface, corresponding to the CD3low phenotype, was detected at to 10 weeks p.i The cells remained CD3low until receptor negative cells began to emerge around months p.i followed...
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Báo cáo toán học: " Inert coupling of IRDye800CW to monoclonal antibodies for clinical optical imaging of tumor targets" potx
Ngày tải lên :
20/06/2014, 21:20
... for photoimmunoconjugates This made us decide to use 89 Zr-labeled mAbs as a starting point to facilitate analytical procedures to study, analyze, and quantify the inertness of dye coupling to mAbs ... 280 nm to measure mAb absorption, at A = 430 nm to measure the absorption of N-sucDf-Fe (III), and at A = 780 nm to measure the absorption of IRDye800CW The chelate -to- mAb and IRDye800CWto-mAb ... without additional toxicology studies being required In a comparable approach, we now aimed the inert coupling of IRDye800CW to mAbs, enabling clinical PID as a complementary tool to radioimmunodetection...
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Báo cáo khoa học: "Hyperoxic acute lung injury and ventilator-induced/associated lung injury: new insights into intracellular signaling pathways" docx
Ngày tải lên :
13/08/2014, 03:20
... alveolar remodeling process during recovery from injury In contrast to the literature on VI/ALI, where increased expression of cytokines and chemokines has been thought to be related to increase ... epithelial cell apoptosis [10], in studies of HALI, overexpression of cytokines or chemokines (for example, TNF-α, IL-1β, IL-6, CXC-chemokine receptor 2, and IL-11), growth factors (for example, ... lung injury and the acute respiratory distress syndrome N Engl J Med 2000, 342:1301-1308 Esteban A, Anzueto A, Frutos F, Alia I, Brochard L, Stewart TE, Benito S, Epstein SK, Apezteguia C, Nightingale...
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Báo cáo y học: " Effect of SWI/SNF chromatin remodeling complex on HIV-1 Tat activated transcription" pps
Ngày tải lên :
13/08/2014, 09:20
... the chromatin remodeling complex, BRG1, to the HIV-1 LTR Effect of acetylated Tat on recruitment of a chromatin remodeling complex The identification of BRG1 as one of the proteins that bound to ... MALDI-TOF mass spectrometry In addition to known factors such as Sp1, TFIIB, and cdk9/ cyclin T1, we also specifically detected the presence of acetylated Tat and members of the SWI/SNF chromatin remodeling ... [34] Cdk9 is analogous to a component of a positive acting elongation factor (P-TEFb) isolated from Drosophila [35], which acts to stimulate promoter-paused RNAPII to enter into productive elongation...
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Báo cáo y học: "Intracellular immunity to HIV-1: newly defined retroviral battles inside infected cells" pdf
Ngày tải lên :
13/08/2014, 09:21
... blocks to N-tropic MLV and EIAV in human cells were thought to be due to the restriction factor (Ref1) [25], and those to HIV-1 and SIV in monkey cells to the lentivirus susceptibility factor (Lv1) ... cytosines and convert them to uracils (C to U transitions) on DNA or RNA (e.g A1) templates During the secondstrand DNA synthesis, these C to U transitions are then converted to those of G to ... frequency of G to A transitions [55,56] In certain strains of HIV-1, up to 60% of all guanidines are replaced by adenines [57] Previously, this G to A hypermutation was attributed to the Page of...
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Báo cáo y học: "Chromatin remodeling and genome stability" ppsx
Ngày tải lên :
14/08/2014, 16:21
... ‘active’ marks It remains to be seen what proteins interact with R3 methylation to achieve this remodeling Moved to expression Taking a step back from the analysis of chromatin remodeling at this level, ... ATPase activity and was shown to interact with ubiquitinated histone H4, and to be able to slide nucleosomes; if its ATPase activity is abolished, cells become sensitive to DNA damage, indicating ... affecting its acetylation lead to increased sensitivity to agents that cause double-strand breaks The persistence of K56 acetylation when double-strand breaks are present is due to the presence of DNA...
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REGULATION OF SRF ACTIVITY BY THE ATP-DEPENDENT CHROMATIN REMODELING ENZYME, CHD8
Ngày tải lên :
24/08/2014, 11:38
... chromatin Heterochromatin has significantly reduced sensitivity to nuclease digestion as compared to euchromatin (Gaszner and Felsenfeld, 2006) In contrast to euchromatin, histones of heterochromatin ... idea of chromatin remodeling has evolved into multiple definitions In general chromatin remodeling can be defined as any event that alters the structure of a chromatin region to change histone-DNA ... transcription factors and the basal transcription machinery in order to initiate transcription Chromatin remodeling complexes thus alter the structure of the chromatin to allow access to 32 these factors...
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Coupling receptor activation to cytoskeleton changes 2
Ngày tải lên :
11/09/2015, 09:57
... inhibitor was from Roche Specific Cdk1 inhibitor –RO3306 and Taxol (Paclitaxel, semisynthetic) were from Calbiochem CK1 inhibitor was from Tocris Purified Bovine Brain tubulin (TL238) was from Cytoskeleton ... CRMP2(480-572) were cloned into pXJ-GST using PCR to introduce HindIII and XhoI cloning sites Full length CRMP1 and CRMP2 were cloned into a modified pET21D His6 vector for expression in bacteria ... was cloned into C-terminal His-tag pcDNA3 vector via BamHI/NotI sites The construct was transfected into HEK293 cells via Lipofectamine 2000 (Invitrogen) for 4hrs before changing into serum-free...
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The study of interactions of transmembrane receptors and intracellular signaling proteins in live cells by fluorescence correlation and cross correlation spectroscopy
Ngày tải lên :
14/09/2015, 14:25
... factor receptor-bound protein 2) bind to the phosphorylated tyrosine residues, and bring the guanine nucleotide exchange factor (GEF) SOS (Son of Sevenless) to the activated receptors The receptor-bound ... to the nucleus and bind to DNA to activate gene transcription, resulting in cell growth and division 1.2.2 Activation mechanisms of the ErbB receptors Two EGFs bind to two receptor subunits to ... factor (TGF)-α, heparin-binding epidermal growth factor (HB-EGF) (41) Neuregulins (NRGs) bind to ErbB3 and ErbB4, but no ligand has been identified to bind to ErbB2 Generally, two ligands bind to...
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