corynebacterium listeria and bacillus
Báo cáo khoa học: Molecular imprinting of cyclodextrin glycosyltransferases from Paenibacillus sp. A11 and Bacillus macerans with c-cyclodextrin pptx
Ngày tải lên :
07/03/2014, 10:20
... A11 and BM was Table Degree of derivatization of the CGTases from A11 and BM obtained with different protein ⁄ itaconic anhydride ratios and remaining cyclization activity Paenibacillus sp A11 Bacillus ... A11 and BM CGTases determined by the 2,4,6-trinitrobenzene sulfonic acid (TNBS) assay were 62% and 65%, respectively The remaining activities of the derivatized A11 and BM CGTases were 90% and ... level of the mature protein is described The native enzymes from Paenibacillus sp A11 (A11) and Bacillus macerans (BM) form CD7 and CD6 as their major products, respectively [13,14] The imprinting...
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Báo cáo khoa học: Jackbean, soybean and Bacillus pasteurii ureases Biological effects unrelated to ureolytic activity docx
Ngày tải lên :
16/03/2014, 16:20
... urease (SBU) and Bacillus pasteurii urease (BPU) [19] for their lethality in mice and for their insecticidal and platelet aggregating activities Kinetic parameters and susceptibility of SBU and BPU ... the insecticidal and ureolytic activities of JBU and SBU Ureases were incubated for 18–24 h at °C with different concentrations of p-HMB (5 lM and 50 lM), dialysed against buffer A and then assayed ... platelets [12–15] JBU and SBU showed similar potency as inducers of platelet aggregation (Fig 6), with EC50 ¼ 22.2 and 15.8 lgÆmL)1 for SBU and JBU, respectively BPU, on the other hand, showed a 20-fold...
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Báo cáo khoa học: Structural studies of thymidine kinases from Bacillus anthracis and Bacillus cereus provide insights into quaternary structure and conformational changes upon substrate binding pot
Ngày tải lên :
23/03/2014, 09:21
... Bacillus thymidine kinase structures U Kosinska et al four deoxyribonucleoside-specific dNKs: cytosolic deoxycytidine kinase (dCK) and TK1 and mitochondrial deoxyguanosine kinase (dGK) and ... dCK [7], and later on hTK1 [8,9] and TK from Ureaplasma urealyticum (Uu-TK) [9] However, Herpes simplex virus type thymidine kinase shares structural and sequential similarities with dNKs and does ... anthracis and B cereus share 96% amino-acid sequence identity Bc-TK consists of 195 amino acids, and Ba-TK is one amino acid shorter The last five and the last four amino acids in Bc-TK and Ba-TK,...
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Tài liệu Báo cáo khoa học: Secondary substrate binding strongly affects activity and binding affinity of Bacillus subtilis and Aspergillus niger GH11 xylanases docx
Ngày tải lên :
14/02/2014, 19:20
... both XBS and XAN involved in substrate interaction were selected based on the crystal structures of XBS and XAN soaked with xylotetraose and xylopentaose, respectively [12] (Fig 1), and were ... = 9.76 · 10)10 M and AzU = 50.8 · 10)10 M and for the wild-type XAN, X6U = 1.02 · 10)10 M, XyU = 7.72 · 10)10 M and AzU = 9.65 · 10)10 M for the activity on X6, Xylazyme AX and Azo-wheat AX, ... xylanases and other glycoside hydrolases will need to be determined Materials and methods Materials All chemicals, solvents and reagents were purchased from Sigma-Aldrich (Bornem, Belgium) and are...
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Tài liệu Báo cáo khoa học: Homologous expression of the nrdF gene of Corynebacterium ammoniagenes strain ATCC 6872 generates a manganese-metallocofactor (R2F) and a stable tyrosyl radical (Y•) involved in ribonucleotide reduction ppt
Ngày tải lên :
15/02/2014, 01:20
... pitch standard (g = 2.0028) A solution of 10 mm CuSO4 in m NaClO4 and 10 mm HCl was used as the standard for spin integration Further EPR conditions are provided in the legend to Fig Q-band EPR ... Microbiology (Pandalai SG ed), pp 15–39 Research Signpost, Trivandrum, India 18 Teshiba S (1989) Production of Nucleotides and Nucleosides by Fermentation, Vol 3, 1st edn Gordon and Breach Science ... from Corynebacterium ammoniagenes and comparison of their gene expression levels in C ammoniagenes and Escherichia coli Biotechnol Lett 25, 1311– 1316 35 Torrents E, Roca I & Gibert I (2003) Corynebacterium...
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Tài liệu Báo cáo khoa học: Structure determination and biochemical studies on Bacillus stearothermophilus E53Q serine hydroxymethyltransferase and its complexes provide insights on function and enzyme memory doc
Ngày tải lên :
18/02/2014, 16:20
... group and contained one monomer in the asymmetric unit Cell dimensions and details of data collection are shown in Table Expression and purification of bsSHMT and E53QbsSHMT The expression and purification ... supported by the Department of Science and Technology and DBT We thank Babu and James for their help during data collection VR and BSB thank the Council for Scientific and Industrial Research, Government ... Gly and Ser external aldimine complexes of E75Q and E75LrcSHMT, the electron density for the ligands was not well defined [15] In contrast, excellent electron density was observed for the ligands...
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Tài liệu Báo cáo khoa học: Biochemical characterization of Bacillus subtilis type II isopentenyl diphosphate isomerase, and phylogenetic distribution of isoprenoid biosynthesis pathways doc
Ngày tải lên :
19/02/2014, 13:20
... template and the oligonucleotides 5¢-TTGGTGGGATCCGTGACTCG AGCAGAACGAAAAAGAC-3¢ and 5¢-GGCTTTGTCG ACTTATCGCACACTATAGCTTGATG-3¢ as primers (restriction sites are underlined and start- and stop-codons ... FMN, mM sodium acetate, and 10.8 mM IPP and 10.8 mM DMAPP, respectively; *, internal standard (acetate) FMN, we observed the appearance of the signals of both methyl groups and of the methine group ... Actinobacteria group (Corynebacterium sp., Mycobacterium sp and Streptomyces sp.), but also in the Bacteroidetes group (Cytophaga hutchinsonii), and some in the a-subgroup (Rhodobacter sphaeroides and Silicibacter...
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Tài liệu Báo cáo khoa học: Relationships between structure, function and stability for pyridoxal 5¢-phosphate-dependent starch phosphorylase from Corynebacterium callunaeas revealed by reversible cofactor dissociation studies doc
Ngày tải lên :
19/02/2014, 16:20
... Analytical enzymes and enzyme substrates were specied in previous papers [1518] All other chemicals were of reagent grade and obtained from Sigma and Fluka Preparation of apo-Cc StP and apo-Ec MalP ... citrate and 0.1 M cysteine hydrochloride, in short, the resolution buffer Various pH values between 5.0 and 8.0 were tested, and a pH of 7.0 was chosen (see below) Prior to the resolution, CcStP and ... R226A and R242A mutants (Table 1), all enzymes were stable for h in the presence of mM potassium phosphate and potassium sulphate Reconstitutions with PLP of apo-Cc StP and mutants thereof, and...
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Tài liệu Báo cáo khoa học: Identi®cation and properties of type I-signal peptidases of Bacillus amyloliquefaciens doc
Ngày tải lên :
21/02/2014, 03:20
... amyloliquefaciens and primers V1, V2, and V3 (Table 2) for regions MKKRFWFLA, VFIDYKVEG, and IVGVISDAE of the B subtilis SipV protein were chosen and found to generate PCR fragments of about 0.5 and 0.4 ... tissue and in a carpel cell layer Plant J 24, 869±882 48 Ash, C., Farrow, J.A., Dorsch, M., Stackebrandt, E & Collins, M.D (1991) Comparative analysis of Bacillus anthracis, Bacillus cereus, and ... genomic DNA of B amyloliquefaciens and the degenerative primers V1/V3 and V2/V3 or W1 and W2, led to the isolation of core DNA fragments of about 0.5 kb (lane b) and 0.4 kb (lane c), or 0.2 kb,...
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Tài liệu Báo cáo Y học: Bivalent cations and amino-acid composition contribute to the thermostability of Bacillus licheniformis xylose isomerase doc
Ngày tải lên :
22/02/2014, 07:20
... (i.e Q, N, S, and T) and an increase in charged amino-acid residues (i.e K, E, and R) in hyperthermophilic proteins As S and T can catalyze the deamination and backbone cleavage of Q and N residues ... 0.981, is based on the elution data of the 200, 150, and 66 kDa protein standards Linear regressions (1) and (2) give molecular masses of 200 and 177.5 kDa, respectively, for BLXI q FEBS 2001 ... a Sephacryl S-300 HR column (1.4 cm £ 160 cm) calibrated with blue dextran and protein standards of 443, 200, 150, and 66 kDa (Sigma Chemical Co., St Louis, MO, USA) The flow rate was 0.2 mL·min21...
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Báo cáo khoa học: Interaction of the general transcription factor TnrA with the PII-like protein GlnK and glutamine synthetase in Bacillus subtilis potx
Ngày tải lên :
06/03/2014, 00:21
... exponential growth phase, and the cells were then washed and resuspended in SMM without nitrate, and finally incubated for a further 20 Samples were taken before and after the shift, and soluble cell-free ... the nitrate and nitrite utilization genes [2], the nrgAB (amtBglnK) operon (ammonium transport) [3], and some other target promoters [1,4,5] TnrA is a negative regulator of glnA and gltAB, encoding ... glutamine and AMP [9] Mutations in TnrA that result in constitutive expression of the TnrA-activated amtB promoter all lie within the C-terminal region of TnrA, and impair the interaction between GS and...
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Báo cáo Y học: Overexpression of a recombinant wild-type and His-tagged Bacillus subtilis glycine oxidase in Escherichia coli pptx
Ngày tải lên :
08/03/2014, 16:20
... sarcosine or sarcosine analogues (monomeric and tetrameric sarcosine oxidases and sarcosine and dimethylglycine dehydrogenases), on D-amino acids (DAAO and DASPO) and on pipecolic acid (PIPOX) (see Table ... P31228 Q63342 NP_106044 NP_057602 Bacillus halodurans Thermoplasma volcanium Mesorhizobium loti Pyrococcus abyssi Corynebacterium sp P-1 Bacillus sp NS-129 Bacillus sp B-0618 Fusarium solani ... (R302, see Fig 2C) On the other hand, and according to the sequence comparison, M261 in GO appears to be located at the position occupied by Y238 and H269 in DAAO and MSOX, respectively This second...
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Báo cáo khoa học: Cloning, expression and characterization of a new aspartate aminotransferase from Bacillus subtilis B3 docx
Ngày tải lên :
14/03/2014, 23:20
... sequence alignment of AATB3 and ATTs from several other organisms (Fig 1), the AATB3 showed 56% identity with B circulans AAT, and 16% and 14% with Bacillus sp YM-2 and T thermophilus HB8 AATs, ... subtilis and B circulans should belong to subgroup Ib Expression and purification of AATB3 and its mutants To produce recombinant AATB3 and the three mutant proteins, the aatB3 gene and its mutants ... AAT and PLP, the Asp232 and Lys270 residues (corresponding to Asp222 and Lys258 in E coli AAT) were replaced with Asn and His using site-directed mutagenesis to obtain the mutants D232N and K270H,...
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Báo cáo khoa học: LmbE proteins from Bacillus cereus are de-N-acetylases with broad substrate specificity and are highly similar to proteins in Bacillus anthracis pot
Ngày tải lên :
15/03/2014, 11:20
... achieved through interaction between the b8-strand of one monomer and the b6- and b7-strands of the other monomer (B) Surface representation of the enzyme and view of the active site from outside The ... pH and temperature optima and were not inhibited by acetate or EDTA BC1534 and BC3461 were activated by CoCl2 and MgCl2, respectively Both enzymes were effective in de-N-acetylating GlcNAc and ... enzymes and DNA-modifying enzymes were purchased from MINOTECH Biotechnology (Heraklion, Greece) and New England Biolabs GmbH The instruments Fluostar Galaxy and Mastercycler Gradient (for PCR and...
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Báo cáo khoa học: The twin-arginine translocation (Tat) systems from Bacillus subtilis display a conserved mode of complex organization and similar substrate recognition requirements doc
Ngày tải lên :
16/03/2014, 04:20
... contains three tatA genes, denoted tatAd, tatAy and tatAc, and two tatC genes denoted tatCd and tatCy The tatAd gene is expressed in an operon with tatCd and these two components form a minimal Tat ... active in E coli and able to export fusion proteins comprising the signal peptides of TorA and DmsA linked to GFP [8] Separate TatAdCd and TatAd complexes were characterized and shown to be very ... (TorA) activity Membrane, cytoplasmic and periplasmic fractions (M, C and P) were prepared and analysed from wild-type MC4100 cells, DtatABCDE cells (Dtat) and DtatABCDE cells expressing B subtilis...
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Báo cáo khoa học: pyr RNA binding to the Bacillus caldolyticus PyrR attenuation protein – characterization and regulation by uridine and guanosine nucleotides potx
Ngày tải lên :
16/03/2014, 06:20
... BcBL2 and BcBL3, and the effects of nucleotides on RNA binding A rapid and convenient filter binding assay [14] was used for many of these experiments Electrophoretic mobility shift assays and sedimentation ... and PRPP) or inhibition (GMP and GTP) of binding of PyrR to BcBL1 and BcBL2 Data are the average of at least two independent determinations To learn more about how PyrR distinguishes purine and ... was determined from both sedimentation velocity and equilibrium sedimentation experiments at high and low protein concentrations and in the presence and absence of 0.1 m NaCl The results of the...
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Báo cáo khoa học: Importance of tyrosine residues of Bacillus stearothermophilus serine hydroxymethyltransferase in cofactor binding and L-allo-Thr cleavage Crystal structure and biochemical studies pot
Ngày tải lên :
16/03/2014, 06:20
... Y61 and increases the distance between the hydroxyl group of Y61 and Ca of the ligand In Gly, l-Ser and l-alloThr complexes with Y51F bsSHMT, the hydroxyl group of Y61 and Ca of the bound ligand ... et al Role of Y51 and Y61 in bsSHMT catalysis PLP content and activity measurements of Y51F, Y61A and Y61F bsSHMTs The purified Y51F and Y61F bsSHMTs were nearly colourless and pale yellow, respectively, ... structural and functional studies on Y51F, Y61F and Y61A bsSHMTs These 4608 studies suggest that Y61 is a possible candidate for proton abstraction from Ca of Gly and 3-hydroxy amino acids, and that...
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Báo cáo khoa học: Interaction of the E2 and E3 components of the pyruvate dehydrogenase multienzyme complex of Bacillus stearothermophilus ppt
Ngày tải lên :
16/03/2014, 18:20
... icosahedral and octahedral complexes: lipoyl domain structures from Bacillus stearothermophilus, Escherichia coli and Azotobacter vinelandii PDH complexes [7–9], and E coli and A vinelandii 2-oxoglutarate ... than 0.10 and 0.02 p.p.m in the 15N and 1H dimensions, respectively) were observed for Asn126, Arg127, Ala131, Gly156 and Glu161 However, for all but the eight and two residues in the N- and C-terminal ... dehydrogenase and B stearothermophilus PDH complexes [12,13]; the octahedral acyltransferase core from A vinelandii PDH [14] and E coli 2-oxoglutarate dehydrogenase [15] complexes and the icosahedral...
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Báo cáo khoa học: Identification, characterization and activation mechanism of a tyrosine kinase of Bacillus anthracis docx
Ngày tải lên :
23/03/2014, 06:20
... et al McsB of Bacillus anthracis B anthracis include Glu120, Glu121 (NEED motif) and Glu212, the catalytic Cys167, and the positively charged Arg125, Arg176 and Arg207 (Figs S1B and S4) The phosphorylation ... the Bacillus cereus group shows that B anthracis appears to lack some of the important histidine kinases (HKs) and response regulators, and contains many truncated, possibly nonfunctional, HK and ... kinases and evolved to its current nonfunctional forms The orthologs of SalA (BAS0147 and BAS3357, showing similarities of 85% and 65%, respectively), MinD (BAS4346, showing a similarity of 90%) and...
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Báo cáo khoa học: Probing the active site of Corynebacterium callunae starch phosphorylase through the characterization of wild-type and His334fiGly mutant enzymes pot
Ngày tải lên :
23/03/2014, 07:20
... annealing and elongation time of and an annealing temperature of 55 °C We used the following pairs of flanking and mutagenic primers, where Eco91I and XagI restriction sites are underlined and the ... absence of ligand (d), in the presence of 20 mM arsenate (s), and in the presence of 20 mM arsenate and mM GL (.); 31P shift for the H334G mutant (,), recorded in the absence of arsenate and at only ... from both the main chain amide and the side chain of Ser Hydrogen bonds between PLP phosphate and the side chains of nearby Lys residues and bound phosphate ligand appear to be stronger in maltodextrin...
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