contraction and excitation of smooth muscle

... comparison of Tm5a with smTm allows study of the effects of replacement of muscle exons 1a and 2a by nonmuscle exon 1b, and comparison of Tm5a with Tm5b allows the effects of exchange of exon to ... of the a-Tm molecule For this purpose, we have used the smooth muscle a-Tm (smTm) and the two a-Tm fibroblast isoforms Tm5a and Tm5b, as shown in Fig All of these a-Tm isoforms have a smooth muscle- like ... Fig Exon structure of smooth muscle a-Tm and the two fibroblast isoforms Tm5a and Tm5b with constitutive exons are shown in white, smooth muscle exons in light grey, and nonmuscle exons in dark...

Ngày tải lên: 19/02/2014, 07:20

... pathophysiology of GCA and the ongoing inflammatory process in the arterial wall The combined use of 2-DE and immunoblotting offers an interesting approach to the identification of target antigens of autoantibodies ... AN, Koteliansky VE, Smirnov VN: Modulation of human aorta smooth muscle cell phenotype: a study of muscle- specific variants of vinculin, caldesmon, and actin expression Proc Natl Acad Sci USA ... catalytic subunit of the human holoenzyme telomerase RT and T antigen of simian virus 40 in a primary culture of VSMCs as previously described [19] Immortalised VSMCs were cultured in Smooth Muscle Cell...

Ngày tải lên: 12/08/2014, 17:22

... compact subintervals of 0, 10 Boundary Value Problems Proof of Theorem 1.5 Suppose that u1 t and u2 t are C 0, positive solutions to 1.1 and 1.2 , and at least one of ≡ them is a smooth positive ... existence and uniqueness of smooth positive solutions to the second-order singular m-point boundary value problem 1.1 and 1.2 A necessary and sufficient condition for the existence of smooth positive ... Province Q2008A03 and the Doctoral Program Foundation of Education Ministry of China 200804460001 References X Du and Z Zhao, “A necessary and sufficient condition of the existence of positive solutions...

Ngày tải lên: 21/06/2014, 20:20

... 43.3% of clenching, and 24.4% of tapping activities based on the count of events; whereas 56.8% of grinding, 37.4% of clenching, and 5.8% of tapping were registered based on the length of events ... time length of bruxism episode was 250 msec of muscle burst in the case of tapping and over 500 msec of burst in the cases of clenching and grinding, and minimum inter-episode time was more than ... Figure Distribution of muscle activity (%MVC) into the different patterns of bruxism Distribution of muscle activity (%MVC) into the different patterns of bruxism Variation of muscle activity (%MVC)...

Ngày tải lên: 11/08/2014, 20:20

... physiology, and neurology of muscle function The action of the muscle being tested, as well as the role of synergistic muscles, must be understood Manual muscle testing is both a science and an art ... Dorsiflexion of the foot and the great toe, plantar flexion of the foot and great toe, quadriceps weakness, and peroneal muscle tests are each indicative of the status of the sciatic nerve and its ... to perform manual muscle testing is a well-trained examiner, using his perception of time and force with knowledge of anatomy and physiology of muscle testing." Regardless of the methods or equipment...

Ngày tải lên: 13/08/2014, 14:20

... Examples of this type of smooth muscle include the ciliary muscle of the eye, the iris of the eye and the piloerector muscle that causes erection of hair In single-unit smooth muscle, a whole group of ... INTRODUCTION A Overview of smooth muscle B Smooth muscle contraction C Smooth muscle origins D Smooth muscle phenotypes E Smooth muscle differentiation markers ... function of smooth muscle cells During the pathogenesis of these diseases, smooth muscle cells have a marked alteration in the expression of many smooth muscle- specific genes and smooth muscle...

Ngày tải lên: 24/08/2014, 13:09

... damage of HIV and TB as well as HIV/TB coinfection Expanding and deploying of intervention programs on HIV infection such as giving out clean needles and condoms Expanding medical gridlines and ... 14,769 and (incubatory oral fluid) techniques; 09 studies with total samples of 6,562 and lung x-ray and incubatory oral fluid techniques; 01 study with total samples of 161 and lung x-ray and Mantoux ... however, the conditions of the two health issues go hand in hand HIV was the main cause of failure in the preventive strategy of TB and vice versa, TB was the main cause of fatalities among HIV...

Ngày tải lên: 04/10/2014, 12:44

... affect smooth Ó FEBS 2002 Fig Ablomin specifically inhibits high K+-stimulated contraction of rat-tail arterial smooth muscle (A) Blockage of high K+-induced contraction by ablomin Smooth muscle ... obtained mg of triflin and mg of latisemin from 500 mg of crude venom, respectively The predicted amino-acid sequences of triflin and latisemin are homologous to that of ablomin (83.7 and 61.5%, ... The effects of tigrin and ablomin on rat tail arterial contraction Fig Isolation of tigrin from the Duvernoy’s glands of R tigrinus tigrinus (A) The extract from Duvernoy’s glands of R tigrinus...

Ngày tải lên: 18/03/2014, 01:20

... EGF (A) -and PDGF (B)-induced contraction of guinea pig open-ring tracheal smooth muscle preparations EGF (A) -and PDGF (B)-induced contraction of guinea pig open-ring tracheal smooth muscle preparations ... of Rho-kinase inhibition on prostaglandin-induced contraction Effects of Rho-kinase inhibition on prostaglandin-induced contraction PGE2 (A) -and PGF2α (B)-induced contraction in the absence and ... EGF and PDGF induce airway smooth muscle contraction through contractile prostaglandins These prostaglandins are presumably produced by the consecutive actions of MEK, cytosolic PLA2 and COX and...

Ngày tải lên: 12/08/2014, 18:22

... via increased transcription and/ or by stabilization of the CTGF transcripts and the role of RhoA GTPase and p38 signaling in such a regulation, nuclear run-on assays and message stability analyses ... transcription rate of the CTGF/CCN2 gene was determined upon stimulation of the cells with S1P, anisomycin or FBS in the absence and in the presence of RhoA GTPase and p38 inhibitors (Y-27632 and SB-203580, ... angiotensin II and prostaglandins stimulate the expression of CTGF/CCN2 efficiently [8,43–45] The role of the cytoskeleton is important, particularly in the compartmentalization of the cytoplasm and organization...

Ngày tải lên: 19/02/2014, 16:20

... Modulation of smooth muscle cell (SMC) proliferation and retinoblastoma protein (Rb) phosphorylation after supplementation with n-3 and n-6 polyunsaturated fatty acids (PUFAs) (A,B) Effects of n-3 and ... control of SMC proliferation 4466 S Bousserouel et al (Eur J Biochem 271) Modulation of cyclin D1 synthesis and hyperphosphorylation of Rb by n-3 and n-6 PUFAs Induction of cyclin D1 is one of the ... proliferation of vascular smooth muscle cells through modulation of various steps of growth signals Lipids 31 (Suppl ), S301–S304 16 Pakala, R., Sheng, W.L & Benedict, C.R (2000) Vascular smooth muscle...

Ngày tải lên: 19/02/2014, 16:20

... induction Effects of a-T and its derivatives on the enhancement by TS of LPS/IFN-induced NO production We compared the effects of a-T and its derivatives TA and TN with that of TS on LPS/IFN-induced ... production with Ro31-2880 and GF109203X were approximately 70 and 30%, respectively Effects of TS on the amount of PKCa We further examined the effects of TS on the amounts of PKCa and PKCb by Western ... upregulation of PKCa DISCUSSION Fig Effects of PKA inhibitors (KT-5720 and H8) and PKC inhibitors (Ro31-8220 and GF109203X) on the enhancement by TS of LPS/IFNinduced NO production in VSMC (A), and Western...

Ngày tải lên: 22/02/2014, 04:20

... both the number of bands and the labeling intensity were increased in growing cells The most prominent increases were in bands of 140, 133, 116, 95, 82, 73 and 56 kDa (Fig 4) Other bands also showed ... Campbell, J.H (1986) Replication of smooth muscle cells in vascular disease Circ Res 58, 427–444 Owens, G.K (1995) Regulation of differentiation of vascular smooth muscle cells Physiol Rev 75, 487–517 ... inhibits the proliferation of human and rat vascular smooth- muscle cells Biochem J 283, 403–408 Saward, L & Zahradka, P (1997) Coronary artery smooth muscle in culture: migration of heterogeneous cell...

Ngày tải lên: 17/03/2014, 09:20

... physical association between c-Jun and STAT5B is provided by the results of the CoIP and super-EMSA of nuclear extracts, and by the results of the ChIP assay of immunoprecipitated chromatin from ... and c-Jun, and STAT5B and AP-1 interaction in AGT gene activation the expression of the AGT gene in VSMCs stimulated with Ang II, the effect of Ang II on the phosphorylation of STAT5 and c-Jun ... phosphorylation of STAT5B and c-Jun, with levels of phosphorylated STAT5B and c-Jun significantly increasing h, and peaking h, after stimulation with Ang II, whereas total c-Jun and STAT5B were...

Ngày tải lên: 30/03/2014, 02:20

... Counter (excitation and emission filters of 530 and 616 nm, respectively) A standard cell suspension of every cell type was prepared in DPBS and kept at -80°C until use The growth fraction of exponentially ... hospital's review board UC arteries and vein endothelial and smooth muscle cell isolation A segment of the UC was clamped at both ends, severed and kept at 4°C for a maximum of 24 h in sterile Hank's Balance ... weight Of the UC vessels, the vein is a classic source of both endothelial and smooth muscle cells (EC and SMC, respectively), mostly because it is a large vessel that can be easily handled [12]...

Ngày tải lên: 18/06/2014, 15:20

... Accumulation of cerebral vascular fibrillar Aβ has been shown to cause marked degeneration and cell death of smooth muscle cells and pericytes in affected larger cerebral vessels and in cerebral ... the levels of IL-6, MMP-2 activation, and loss of smooth muscle α actin, processes that precede HCSM cell death and likely contribute to inflammation and loss of vessel wall integrity in CAA Methods ... proliferating and degenerating smooth muscle cells Amyloid 1994, 1:8-16 Bailey TL, Rivara CB, Rocher AB, Hof PR: The nature and effects of cortical microvascular pathology in aging and Alzheimer's...

Ngày tải lên: 19/06/2014, 22:20

... mRNA expression of collagenase (MMP1) and both gelatinases (MMP2 and MMP9) (CSx) compared with controls (ctrl) exceeding elastolysis and collagenolysis and thus a dramatic loss of structural integrity ... Histological examination of the resected tissue specimen (Fig 3) demonstrated an occult inflammatory process with massive inflammatory infiltration and loss of smooth muscle cells particularly ... lamellae and collagenous matrix were degraded (Fig 3A) Foci of matrix deterioration in deeper medial areas collocated with clusters of inflammatory cells (Fig 3B and 3C) MMP1 (Fig 3D) and MMP9...

Ngày tải lên: 10/08/2014, 09:22

... can increase the smooth muscle cell volume and change the contractile properties of smooth muscle cells and airway reactivity [22-24], as well as to promote ASMC proliferation and participate ... with a double -excitation microfluorimeter The light emitted by the cells at 510 nm during excitation at wavelengths of 340 and 380 nm was recorded The ratio of the intensities of emission (R340/380) ... as a measure of [Ca2+]i For each image, regions of interest were defined within single cells, and the average fluorescence intensity of each region of interest was measured The role of WIN62577...

Ngày tải lên: 11/08/2014, 03:20

... can increase the smooth muscle cell volume and change the contractile properties of smooth muscle cells and airway reactivity [22-24], as well as to promote ASMC proliferation and participate ... with a double -excitation microfluorimeter The light emitted by the cells at 510 nm during excitation at wavelengths of 340 and 380 nm was recorded The ratio of the intensities of emission (R340/380) ... as a measure of [Ca2+]i For each image, regions of interest were defined within single cells, and the average fluorescence intensity of each region of interest was measured The role of WIN62577...

Ngày tải lên: 11/08/2014, 06:23

... measurements and algorithms to overcome the instability of excitation sources and counteract the manipulation by the user of pinhole size, detector gain, amplifier offset, amplifier gain and excitation ... binding of cyclic AMP to Epac and a subsequent conformational change of Epac leading to an increased distance between the CFP and YFP termini of the probe and a subsequent loss of FRET The trace of ... with a laser emitting at 440 nm and the emission of CFP and YFP were detected by rapid switching of 470 nm and 535 nm bandpass filters positioned in a filter wheel and the FRET ratios measured as...

Ngày tải lên: 12/08/2014, 13:22