contamination analysis and control

... Components and Analysis of Cellular Processes by Immunocytochemistry Immunostaining of Voltage-Gated Ion Channels in Cell Lines and Neurons – Key Concepts and Potential Pitfalls Elke Bocksteins, Andrew ... of Cellular Components and Analysis of Cellular Processes by Immunocytochemistry Immunostaining of Voltage-Gated Ion Channels in Cell Lines and Neurons – Key Concepts and Potential Pitfalls 1Department ... decreased staining intensity and possibly an increase in the background ‘noise’ of the stain On the other hand, incomplete fixation will not halt proteolytic and other enzymatic and metabolic processes...

Ngày tải lên: 28/06/2014, 08:20

... Mutations and amplification of the androgen receptor gene, without loss of gene expression, play a key role in the development of advanced, androgen-independent prostate cancer [4] Methylation of the androgen ... human and mouse linker histones Speciation events are indicated by blue dots and gene duplication by red dots HIST1H1C and HIST1H1E are the genes that code for human linker histones H1.2 and H1.4, ... phosphorylations on H1.2 at Thr31 and Ser36, on H1.3 at Ser37, and on H1.4 at Ser2, Thr4, Thr18, Ser27, and Ser36 In comparing the work of Wisniewski et al [43] (Table 1) and Garcia et al [42], it can...

Ngày tải lên: 18/02/2014, 08:20

... Biosciences), which absorb and emit in higher wavelength light (ex/em, 774/789 nm), and investigated their applicability in cell labeling and in vivo imaging Results and discussions Preparation ... Biotechnology and Biomaterials, BME, Southeast University, Nanjing 210096, China Authors’ contributions YH, YL, ZC and NG synthesized nanoparticles; YL, YH and JW performed the cell labeling and the ... a carboxyl and amine-reactive cross-linker, which creates an amide bond between carboxyl and amino groups [50] In this study, the DMSA coating was important for the water solubility and NIRF labeling...

Ngày tải lên: 11/08/2014, 00:22

... both in vitro and in vivo, and its applications in different areas related to proteomics and chemical biology, namely protein microarray technologies for large-scale protein analysis and live cell ... and Methods 2.1 Construction of expression plasmids EGFP and GST were PCR amplified from pEGFP (Clontech, USA) and pGEX-4T1 (Pharmacia Biotech, USA) respectively, and cloned into the pTWIN1 and ... Sap I and Pst I sites of the pTWIN vector The target gene sequence is in lower case letters Following digestion of the vector and PCR products by endonucleases Sap I and Pst I, the insert and vector...

Ngày tải lên: 08/11/2015, 16:30

... dietary GI and GL and IR (measured by HOMA-IR) 27 Table 3: Intervention studies in children and adolescents examining the effect of dietary GI and GL on IR (measured by HOMA-IR and ALT) ... Europe and around the World RESIST Researching Effective Strategies to Improve Insulin Sensitivity in Children and Teenagers SD, SDS Standard deviation, standard deviation score SE Standard error ... recall, and HOMA-IR values six years later in 233 girls and boys [180] (Table 2) 26 Table 2: Observational study in children and adolescents examining the relation between dietary GI and GL and IR...

Ngày tải lên: 25/11/2015, 14:52

... Carcass composition and meat quality of crossbreds between F1(LandracexYorkshire) sows mated with LandracexDuroc (omega) and PietrainxDuroc (Pidu) Crossbred Boars Journal of Science and Developement ... Piétrain and hybrid boars 2.3 Statistical analysis Growth performance (BW and ADG) were analysed using a linear model including the different genetic groups (PiDu25, PiDu50, PiDu75, PiCC and PiCT) ... Summer, Autumn and Winter) were the only effects included in the model Table Results of the effect of genetic group (PiDu 25, 50 75, PiCC and PiCT) and season on growth performance and semen quality...

Ngày tải lên: 28/08/2013, 11:59

... dissolved Al and Mn became lower The pH value further increased and it reached basic conditions in the reservoir, and dissolved Al and Mn were removed more from the water On the other hand, the removal ... growth chamber under dark condition, and the temperature was maintained constant between 10°C and 30°C RESULTS AND DISCUSSION Materials runoff characteristics and variation in the reservoir Fig ... between pH and heavy metals such as dissolved Al, Mn and Zn are shown in Fig Although the concentrations of dissolved Al and Mn in the leachate were high, pH increased through the stream and the...

Ngày tải lên: 05/09/2013, 10:17

... newspapers and 12 short stories 3.6 DATA ANALYSIS With the collected data, we carry out the analysis of ESVs and VSVs in terms of the syntactic and semantic features The analysis results of the ESVs and ... Literature Review and Theoretical Background; Chapter is Methods and Procedure; Chapter is Findings and Discussions; Chapter is Conclusions and Implications Chapter LITERATURE REVIEW AND THEORETICAL ... data of ask, demand, order, propose, recommend, request, require and suggest from English and Vietnamese magazines, short stories, some dictionaries and the Internet - Clarifying and choosing data...

Ngày tải lên: 26/11/2013, 13:17

... understand the meanings of these FSs or quotations, the readers not only have experience in love and its sweetness and bitterness but also understand the semantic and syntactic features and the ... semantic and syntactic features of EFLSs and their Vietnamese translation 5 - Helping users of English and Vietnamese get access to FSs from different viewpoints and understand more about them and ... and revised and augmented in 1937, for its eleventh edition by Christopher and Louella), Jehiel’s Dictionary of Quotation (first published in 1881, and revised and enlarged in 1927 by Kate) and...

Ngày tải lên: 26/11/2013, 13:21

... Netherlands and Austria is quite substantial and is approximately - 0.2; for France and Belgium it is smaller, namely - 0.005 For Italy, Ireland, Spain, Portugal and Greece is not significant and ... protection and bond Most sovereigns Buy CDS protection and bond Corporates since crisis Empirical analysis on the basis during the crisis so far only covers corporate bonds Fontana (2010) and Barot and ... Lead-lag analysis of bond spreads and CDS 3.5 Regression analysis of the basis 3.6 Further results for the regression analysis of the basis 15 15 17 18 20 22 Conclusions 26 Figures and tables...

Ngày tải lên: 16/02/2014, 02:20

... of the mutants for UTR1, YEF1 and POS5 The mutants and WT cells were treated and spotted on SD and YPD solid media as described in Fig 4A and were grown at 30 °C and 37 °C as indicated Discussion ... On the other hand, no activity was detected in the presence of mm Zn2+, Fe2+, Cu2+, and monovalent metal ions (Na+ and Li+) NADPH and NADH slightly inhibited Yef1p; however, NADP and intermediates ... the pos5 cell on SG solid medium and on and in solid and liquid media lacking arginine (Fig and Table 6), thereby supporting the partial contribution of Utr1p and Yef1p to mitochondrial functions...

Ngày tải lên: 20/02/2014, 01:20

... of bovine ZPA and porcine ZPB [10,11] and ZPC [13] in the ZP domains are shown by filled black boxes Arrows and C indicate the linkage sites of sperm-ligand carbohydrate chains and positions of ... Reverse-phase HPLC of N-glycopeptides retained to LCA and ConA agarose Reduced and carboxymethylated zona protein mixture was cleaved by trypsin and BrCN and applied to an LCA agarose column The flowthrough ... acetonitrile (broken line) in 0.1% trifluoroacetic acid (A and B) Tryptic peptides; (C and D) BrCNpeptides; (A and C) LCA binding fraction; (B and D) ConA binding fraction Peptides were detected at...

Ngày tải lên: 21/02/2014, 03:20

... price and production controls In 2001, the New Zealand Dairy Board, New Zealand Dairy Group, and Kiwi Cooperatives merged to create a single entity, the cooperative Fonterra Fonterra handles, ... output and input prices, and new sources of demand growth (exports, functional nutrients, pharmaceutical products), the models may over simplify and miss the obvious impacts of supply control ... imitation and substitute dairy products Supply management programs have constrained dairy industry and job growth in the EU and Canada and created an economic incentive for imports o Slow domestic and...

Ngày tải lên: 22/02/2014, 05:20

... G13 variants, G19 and G19 h all contained an R & R consensus sequence, and they exhibited significant sequence similarity (G19 and G13, 58% identity; G19 h and G13, 48%; G19 and G19 h, 64%) A ... 16-residue acid signal peptide Two cDNA variants were isolated and designated basic QH4A (Pro36 and His83) and QH4B (Leu36 and Tyr8) (AB201766 and AB201767) The cDNA of basic QH10 encoded a 110-residue ... of HMM and LMM chitin-binding proteins Basic G13 and G19, acidic DE25 and DE29 and S37 were shown by RT-PCR to be expressed predominantly in epidermis (Fig 7) In contrast, basic Y6, Y7 and QH4...

Ngày tải lên: 07/03/2014, 21:20

... internal standard We analyzed the retinol standard solution by HPLC and did not detect any all-trans-retinal contamination A standard curve for retinol was constructed for each experiment and we ... activity and the activity bands for corresponding peaks are labeled with arrows In this gel, peak appeared as a doublet (band a and b) Each peak was further separated on nondenaturing PAGE and the bands ... the two bands in peak (Fig 4) The two bands for peak were individually sequenced and the peptides identified for both the bands are listed below The peptides that were common to both the bands are...

Ngày tải lên: 08/03/2014, 10:20

... SiNWs and the metal–SiNWs composite materials, and make the processes more cost-effective bin Yvon T64000), and X-ray photoelectron spectroscopy (XPS, Perkin–Elmer PHI-5702) Experimental Results and ... and an ethereal solvent, tetrahydrofuran (THF), reported in detail elsewhere [14,15] This polymer is composed of a randomly constructed network of tetrahedral hybridized phenylcarbyne units and ... the tip of SiNWs film, shown in Fig 2b, reveals that these wires consist of Si, C and O, and further quantitative analysis shows that the atomic ratio for Si:C:O is ca 1.7:7.3:1, indicating that...

Ngày tải lên: 16/03/2014, 15:06

... The morphologies of the as-prepared R-FeOOH and R-Fe2O3 nanorods were further investigated by TEM and HRTEM Figure 3a and b shows the representative TEM and HRTEM micrographs of the as-prepared ... R-Fe2O3 nanorods Figure (a, b) TEM and HRTEM images of R-FeOOH nanorods (c, d) TEM and HRTEM images of porous R-Fe2O3 nanorods (e) The corresponding ED pattern and (f) HRTEM image of a single R-Fe2O3 ... compared with those of standard compounds reported in the JCPDS data file Transmission electron microscopy (TEM) and high-resolution transmission electron microscopy (HRTEM) analysis were carried...

Ngày tải lên: 19/03/2014, 16:48

... described? Data analysis 6.1 Within-CASE Analysis of Swed and SEB Banks 6.1.1 CASE-1 (Swedbank) 6.1.2 CASE-2 (SEB) 6.2 within CASE analysis of two bank customers 6.3 cross CASE analysis two banks ... understanding of the studied object or objects And to further add or investigate, interpret, and understand the phenomena by the means of an inside perspective As the number of objects are limited and ... differences and similarities existing in both of them and a comparison based on our thesis perspective 4.6 DATA ANALYSIS According to Zikmund (2000) data analysis is the request of reasoning to understand...

Ngày tải lên: 22/03/2014, 21:20

... 5, 7, 8, 11 and 12 By contrast with only one OsTLP found on chromosomes 3, 4, 7, 8, 11 and 12, three (OsTLP5, OsTLP9 and OsTLP12), two (OsTLP8 and OsTLP13) and three (OsTLP1, OsTLP10 and OsTLP11) ... OsTLP12 and OsTLP5) and part of the long arm of chromosome (OsTLP11, OsTLP10 and OsTLP1), part of the short arm of chromosome (OsTLP14) and part of the long arm of chromosome (OsTLP7), and part ... pairs (OsTLP6 ⁄ and OsTLP7 ⁄ 14) may be the result of recent segmental duplication of chromosomes 11 and 12 and chromosomes and 7, respectively [13,14] With regard to OsTLP8 and OsTLP13, they...

Ngày tải lên: 23/03/2014, 07:20