construction modularity analysis and manual curation

... ChIP and analyzed the data TAE carried out ChIP-chip of H3K9 and H3K27 methylation SS helped to draft the manuscript KI and JDL designed experiments and wrote the manuscript All authors read and ... bases covered by repetitive sequences are plotted The bottom and top of boxes indicate the 25th and 75th percentiles, respectively, and bands in the boxes indicate medians Whiskers indicate the lowest ... USA), and then amplified by PCR with single-end PCR primers (Table S5 in Additional file 2) Amplicons were loaded to a 2% agarose gel, and DNA between 300 and 500 bp (LEM-2, control IgG and input...

Ngày tải lên: 09/08/2014, 22:23

... target and novel visual stimuli in children of alcoholics Alcohol 1998; 15:119-36 92 Porjesz B And Begleiter H Human brain electrophysiology and alcoholism In: Tarter R and Thiel DV, Eds Alcohol and ... mass is subject to contention, however MRI imaging and analysis by Hopper et al [68] showed no significant connection between gender or handedness and callosal size; the information available did ... inverse relation between age and callosal size Conversely, a subsequent review of MRI and post-mortem reports indicated a callossal volume relationship to handedness and gender [69]; the clinical...

Ngày tải lên: 02/11/2012, 11:08

... The Netherlands Natural disasters have increased in frequency and severity during the last few decades, causing considerable economic damage and loss of life A combination of climate and socio-economic ... Climate change and the frequency and severity of natural disasters In addition to socio-economic developments, climate change may increase the intensity and severity of weather extremes and contribute ... BOTZEN1, * AND J C J M VAN DEN BERGH2 Institute for Environmental Studies, Vrije Universiteit, Amsterdam, The Netherlands ICREA, Barcelona, Spain; Institute of Environmental Science and Technology...

Ngày tải lên: 07/10/2012, 15:57

... kind and peaceful strong and brave attractive and handsome thoughtful and wise talented and artistic popular and well-liked funny and amusing rich and influential truthful and sincere strong and ... Mondays and Wednesdays b Tuesdays and Thursdays He has three classes on a Mondays and Wednesdays b Tuesdays and Thursdays On the weekends, he a plays golf b plays soccer He watches movies and ... clear in the hills but foggy in the valley a It’s hot and humid b It’s cool and dry c It’s hot and dry d It’s mild and cool a It’s overcast and foggy b It’s raining heavily with strong winds c...

Ngày tải lên: 14/12/2013, 15:07

Ngày tải lên: 18/12/2013, 21:17

Ngày tải lên: 18/12/2013, 21:17

... available and within the range The following example shows how to configure manual switch: Router(config)#network-clock switch manual interface tenGigabitEthernet 7/1 t1 • Clear Manual and Force ... Bandwidth – Two priority queues per policy – The set cos command, set cos-inner command, set cos cos-inner command, and set cos-inner cos command – WRED aggregate – Queue-limit SUMMARY STEPS enable ... Manual Switch: Forcefully select a synchronization source provided the source is available and within the range The ES+ is a family of fixed-port SyncE line cards supporting 20 and 40 Gbps bandwidth...

Ngày tải lên: 25/01/2014, 11:20

... Fig RT-PCR analysis of TMPRSS13 and HAI-1 mRNA in human carcinoma cell lines Total RNA was isolated from cultured A549, LoVo, MKN45, MKN74 and HepG2 cells, and subjected to RT-PCR analysis The ... immunoblot analysis of the reaction product using the anti-c-Myc IgG showed a band of 37 kDa under reducing conditions (Fig 1B), and that of 67 kDa under nonreducing conditions (Fig 1C) The 37 kDa band ... conditions and stained with Coomassie Brilliant Blue The incubation generated two main bands of  60 and 32 kDa (Fig 4A) The sizes corresponded 4892 Fig Proteolytic conversion of pro-HGF by TMPRSS13 and...

Ngày tải lên: 15/02/2014, 01:20

... detoxification and homeostasis The effects on the ligand environments of cadmium and zinc upon deletion of metallothioneins were investigated by comparative analysis of XANES spectra (Fig 3) and EXAFS ... to CeMT-1 and CeMT-2 if presented with Zn : Cd ratios as encountered by C elegans Using a Cd : Zn ratio of 33 : [21 nm Cd(II) and 0.7 lm Zn(II)] and 0.1 lm of CeMT-1 and CeMT-2 each, and the stability ... Table Metal to protein stoichiometries for CeMT-1 and CeMT-2 metalloforms determined by mass spectrometry and elemental analysis Theoretical and observed mass are given for the major species...

Ngày tải lên: 16/02/2014, 15:20

... concentration after stimulation with EGF and PMA together on the one hand, and the sum of the ERK-PP concentrations after stimulation with EGF and PMA separately on the other hand, was not constant, but increased ... NaCl/Pi and washed Fig Biphasic ERK-PP time profile induced by EGF or PMA alone and synergistic ERK phosphorylation induced by EGF and PMA together Cells were serum-starved for three days and subsequently ... Raf1 phosphorylates MAPK/ERK kinase (MEK) and 2, which in turn phosphorylate ERK1 and ERK2 Phosphorylated ERK (ERK-PP) has several different cytoplasmic and nuclear targets Transcription factors...

Ngày tải lên: 19/02/2014, 16:20

... gelsolin S2 sequences (and homologue S2 equivalents) within residues 197–226 (including the long helix of the domain) and within residues 161 – 172 (including the A strand and the AB loop in S2) ... fragments [19] of 31 and 16 kDa (1 –225 and 226–375 sequence, respectively) As shown in Fig 5, digestion of the cross-linked actin peptide complex reveals two faint bands at 33 kDa and 46 kDa which ... 338–348, and the other to two helices and one turn located in the 356–375 sequence The corresponding peptides (339– 349, 347–365, 356–375 and 360–372) were coated to plastic We observed (Fig 8A and...

Ngày tải lên: 22/02/2014, 07:20

... underlined An EF-hand consensus sequence is placed between the aligned EF-hand I and EF-hand II sequences [3] The helices are shown as boxes with conserved hydrophobic (h) and other amino acids ... calcium-binding ligands are designated by their coordinates: x, y, z, -y, -x and -z [60] Residues discussed in the text are given in bold (G34, K60 and G81 of CR I –II and G29, G66, R68, G71 and G74 of ... occurred and a lower band, most probably GST, was observed We observed that CR, but not GST (also the lower bands of lanes and 3), binds 45 Ca under the conditions used GST–CR(1–60) and GST–...

Ngày tải lên: 22/02/2014, 07:20

... kidney, stomach and heart, and its association with liver and kidney mitochondria has been demonstrated by electron microscopy [6] GSTK1 transcript tissue expression is similar in the rat and in the ... interface GSTK-1 and GSTK-2 are localized in peroxisomes and mitochondria, respectively The analysis of GST kappa transcript levels has shown a ubiquitous expression in human [4] and mouse [6] tissues ... elegans A A' E B F C G D E' H Fig Analysis of expression pattern of gstk-1 and gstk-2 in embryo and adult C elegans Projection of confocal images of GFP::GSTK-1 (A–D) and GSTK-2::GFP (E–H) at different...

Ngày tải lên: 16/03/2014, 03:20

... considerations Animal weights, feed and water intakes, and dose Nutritional and environmental considerations Sample collection Residue analysis Radiochemical analysis Analysis of the marker residue ... Seymour, Craig A Smitley and Sabrina X Zhao 509 Introduction Outline of method Reagents and standards Equipment and supplies Sampling and preparation Extraction procedure Manual procedure 509 510 ... Relationship of pesticide residue analysis, regulation, and risk assessment Who does residue analysis and why Challenges References xli xliii 1 Regulatory guidance and scientific consideration for...

Ngày tải lên: 17/03/2014, 02:20

... house and disappeared under the dark archway For Sora Nanna and Stefanone, her husband, were rich people for their station, and their house was large and was built with an arch wide enough and ... iron trestles, and wooden stools of well-worn brown oak, and tables painted a vivid green, and primitive lithographs of Saint Benedict and Santa Scholastica and the Addolorata And there were ... of maize and wheat, waiting to be picked over before being ground in the primeval household mill And there were hams and sides of bacon, and red peppers, and bundles of dried herbs, and great...

Ngày tải lên: 17/03/2014, 13:20

... corresponding to GST-FL (upper band) and GST (lower band); Nt lane, GST-Nt peptide (upper band) and GST (lower band); Ct lane, only one band (GST-Ct peptide) (B) SDS ⁄ PAGE analysis of purified recombinant ... amplification of the FL peptide; F1 and R2 was for the Nt peptide, and F2 and R1 was for the Ct peptide The PCR products were digested with BamHI and EcoRI, and ligated to pGEX-2TK vector (Amersham ... peptides showed two bands, each with the lower band corresponding to a 27 kDa glutathione S-transferase (GST) protein, with the upper band corresponding to 56 kDa for GST-FL and 40 kDa for GST-Nt...

Ngày tải lên: 23/03/2014, 06:20

... both Spd and Spm substrates In particular, HvPAO1 enzymatic activity showed an optimum at pH 7.0 for Spm and Spd oxidation and Km values of 0.89 · 10)5 m and of 0.50 · 10)5 m for Spd and Spm substrates, ... blot analysis of ZmPAO, HvPAO2 and HvPAO1 ZmPAO and HvPAO2 were purified as previously described [4,5] HvPAO1 was purified and in vitro synthesized as described in Experimental procedures Analysis ... features of purified and in vitro synthesized HvPAO1 enzyme RT-PCR analysis was also carried out on different ear tissues and internodes Constructs containing HvPAO1, HvPAO2, ZmPAO1 and ZmPAO2 promoter...

Ngày tải lên: 23/03/2014, 10:21

... handler truy cập handler (được gọi parent) với toàn tag Các tag handler phải cài đặt hai giao tiếp Tag BodyTag Các giao tiếp dùng để lấy đối tượng Java tồn tạo tag handler Để định nghĩa tag handler ... Phần mô tả thuộc tính tag handler mô tả thư viện tag, trình bày cách phát triển tag handler element mô tả thư viện loại tag giới thiệu phần trước Tag handler Tag handler đối tượng gọi lên JSP ... tính tag handler Đối với thuộc tínch tag, phải định nghĩa theo phong cách JavaBean, tức phải có phương thức get/set[Attribute] tag handler Hãy nhớ rằng, thuộc tính đặt tên id tag handler kết...

Ngày tải lên: 27/03/2014, 22:31

... Textile, Clothing and Footwear Mechanic Metal Fitters and Machinists nec Engraver Gunsmith Precision Instrument Maker and Repairer Saw Maker and Repairer Watch and Clock Maker and Repairer Engineering ... Schedule and Australian Government Department of Immigration and Citizenship OCCUPATION Judicial and Other Legal Professionals nec Careers Counsellor Drug and Alcohol Counsellor Family and Marriage ... 2012– Schedule and Australian Government Department of Immigration and Citizenship OCCUPATION Wall and Floor Tiler Business Machine Mechanic Communications Operator Cabler (Data and Telecommunications)...

Ngày tải lên: 29/03/2014, 23:20

... (compare lanes and 1) and the same holds true for the GST–SAFB1CDRE protein (lanes and 4), whereas SAFB2 barely binds to SRPK1a (lane 6) but almost as tightly as SAFB1 to SRPK1 (compare lanes and 1) ... (lane 6) As shown in Fig 6B, both GFP–SAFB1 and GFP–SAFB2 proteins bind to both kinases (left and right panels, lanes 2, and 5, 6), unlike GFP (lanes and 4) which does not bind by itself However, ... BamHI site and reverse: 5¢-TTGAATTCTCAGTAGCGGCGAGT GAA-3¢, containing the underlined EcoRI site The PCR fragment was digested with BamHI and EcoRI, repurified and subcloned into the BamHI and EcoRI...

Ngày tải lên: 30/03/2014, 01:20