construction expression and purification of chimeric protein reagents based on ig fc regions
Optimization of expression and purification of HSPA6 protein from Camelus dromedarius in E. Coli
Ngày tải lên :
24/10/2016, 14:24
... Inducer concentration optimization A higher IPTG concentration has no significant effect on the yield of cHSPA6 protein Therefore, for further optimization experiments a 40-fold lower IPTG concentration ... that the optimal induction at the mid exponential phase produced high levels of soluble proteins with high cell density Figure Effect of pre-induced growth on the expression of cHSPA6 Culture was ... media optimization LB media showed relatively higher growth rate under induced culture conditions resulting in higher volumetric yield Figure Effect of media on the overexpression of cHSPA6 Four...
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Báo cáo khoa học: Expression and characterization of the protein Rv1399c from Mycobacterium tuberculosis A novel carboxyl esterase structurally related to the HSL family docx
Ngày tải lên :
07/03/2014, 16:20
... Results and Discussion Cloning and expression of Rv1399c The Rv1399c gene product encodes a 318 amino acid protein with a molecular mass of 31.7 kDa and a calculated pI of 4.53 and belongs to ... characterization, the Lip term could be confused to refer to a NLH, and the original accession number should be used to avoid confusion Here we report the cloning, expression and refolding of the M ... Inclusion bodies after washing Ni ± chelating Sepharose and concentration Refolding by dilution (T0) Refolding by dilution (day two) Concentration and centrifugation Desalting column and concentration...
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Báo cáo hóa học: " Bacterial-based systems for expression and purification of recombinant Lassa virus proteins of immunological relevance" doc
Ngày tải lên :
20/06/2014, 01:20
... class-specific reagents C 188 98 62 NP-MBP NP 49 MBP 38 Figure 2(DE3) cells transformed with construct pMAL-c2x:NP Expression and purification of LASV NP from E coli Rosetta Expression and purification of ... GP1-MBP MBP 38 28 17 GP1 14 Expression and purification of LASV GP1pMAL-c2x:GP1 Figure gami cells transformed with construct from E coli Rosetta Expression and purification of LASV GP1 from E coli ... classification of LASV as a National Institutes of Allergy and Infectious Diseases (NIAID) Category A pathogen and biosafety level-4 (BSL4) agent Expression and purification of E coli-generated LASV proteins...
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báo cáo hóa học: " Recombinant expression and purification of the 2,5-diketocamphane 1,2-monooxygenase from the camphor metabolizing Pseudomonas putida strain NCIMB 10007" potx
Ngày tải lên :
21/06/2014, 02:20
... units per milligram (U/mg) protein One unit is defined as the amount of enzyme that catalyzes the oxidation of μmol of substrate per minute Results Cloning, expression and purification of 2, 5-diketocamphane ... mg of pure enzyme were obtained (Jones et al 1993) In this work mg of pure protein were achieved out of a 400 mL culture, which highlights the advantages of recombinant expression and the fusion ... (2009) Cloning, expression, characterization, and biocatalytic investigation of the Kadow et al AMB Express 2011, 1:13 http://www.amb-express.com/content/1/1/13 Page of 4-hydroxyacetophenone monooxygenase...
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Báo cáo y học: " Production and purification of VP2 protein of porcine parvovirus expressed in an insectbaculovirus cell system" docx
Ngày tải lên :
11/08/2014, 21:21
... fragment was about 1840 bp 3.2 Expression and purification of polyhistidine-tagged VP2 of PPV We have previously shown that formation of chimeric VLPs of PPV fusion constructs is feasible in pET-PPV ... intensive and costly, with the additional hazard of requiring the handling of large quantities of infectious virus [21] Economic and safety considerations, as well as practical limitations associated ... H-z: [Expression of porcine parvovirus vp2 gene and construction of virus like particles (Chinese)] Virologica Sinica 2006, 21:148-152 Bergeron J, Hebert B, Tijssen P: Genome organization of the...
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Báo cáo y học: "Cloning, expression and characterization of gE protein of Duck plague virus" doc
Ngày tải lên :
12/08/2014, 04:20
... BL21(DE3) and Rosseta cells were individually transformed with the positive recombinant plasmid and used for protein expression Expression and Purification of the recombinant protein Expression of this ... BL21(pLysS) (Fig 2A) And the expression level of the fusion pET32a/DPV-gE protein at 30°C was more than at 25°C and 37°C (Fig 2B) The different concentrations of IPTG showed apparent diversity in Figure ... DNA marker 2000 Page of 11 Expression and purification of the gE recombinant protein To obtain a highly expressed level of pET32a/DPV-gE protein, the recombinant expression vectors pET32a/ DPV-gE...
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Design, optimization and applications of novel electrochemical sensors based on prussian blue
Ngày tải lên :
10/09/2015, 09:13
... crosssectional views of (a) a PB-PGE based on the design of encasing the pencil lead with an insulating layer and (b) a PB-PGE based on the design of direct immersion of a fixed length of the pencil ... (blue) and Epc sub (red) towards logarithm of K+ concentration (inset of a) and logarithm of Na+ concentration (inset of b) S = electrode slope towards the logarithm of the relevant cation concentration ... of Epc towards (a) the logarithm of K+ concentration in (i) absence of Na+ with slope of ca 60 mV (ii) presence of 50 mM Na+ with slope of ca 120 mV; and (b) the logarithm of Na+ concentration...
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Synthesis and characterizations of polymer electrolyte membranes based on aliphatic ionomers
Ngày tải lên :
10/09/2015, 15:49
... Separation of Random and Block Ionomers in Solution .33 2.3.2.2 Phase Separation of Random and Block Ionomers in the Solid State 36 SYNTHESIS AND CHARACTERIZATION OF ACRYLIC RANDOMIONOMER ... in aliphatic PEMs Figure 2.8 A PEM design based on ionic cross-linking of aliphatic diblock ionomers (A), and its proton conductivity as a function of the weight fraction of SA (B) (Do Kyoung ... Synthesis of New Alcohol-Resistant Alternative PEMs based on Aliphatic Random Ionomers (b) Structural Characterizations of Random Aliphatic Ionomers and Investigations of the Effects of Hydrophobic...
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Báo cáo khoa học: Molecular cloning, expression and characterization of protein disulfide isomerase from Conus marmoreus pdf
Ngày tải lên :
07/03/2014, 05:20
... the cPDI was inserted into an expression vector, pET24a, which contains an N-terminal His6 tag Expression and purification of cPDI and hPDI The expression plasmid of cPDI was transformed into BL21 ... compilation ª 2007 FEBS Z.-Q Wang et al higher than those of glutathione (Table 2) The concentrations of GSH and GSSG in the ER lumen are in the millimolar range, whereas the concentration of PDI ... O-glycosylation, bromination of tryptophan, hydroxylation of proline, and l- to d-epimerization, may also facilitate the folding of conotoxins [7] Another mechanism to improve the folding of conotoxins...
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Báo cáo y học: "α Does protein kinase R mediate TNF-α- and ceramide-induced increases in expression and activation of matrix metalloproteinases in articular cartilage by a novel mechanism" pps
Ngày tải lên :
09/08/2014, 01:23
... increase in the expression and activation of MMP-2 and -9 The low level of expression of MMP-9 within our control explants may represent activation of stress response pathways in chondrocytes that ... until the zones of proteolysis had cleared The addition of molecularweight markers and conditioned media to the gels facilitated identification of the enzymes and allowed comparisons between ... the induction of c-jun and c-fos and AP-1-dependent transcription of MMP genes [10] In addition, triggering of the ceramide pathway in human keratinocytes results in overexpression of MMP-9 [11]...
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báo cáo khoa học: "The expression and role of protein kinase C (PKC) epsilon in clear cell renal cell carcinoma" ppt
Ngày tải lên :
10/08/2014, 10:21
... promotion of cell proliferation, escape from apoptosis, and dysregulation of cellular adhesion and migration The invasion of tumor cells to surrounding tissues and spreading to distal sites rely on ... transfection and Huang et al Journal of Experimental & Clinical Cancer Research 2011, 30:88 http://www.jeccr.com/content/30/1/88 Page of Figure Effects of PKCε knockdown on migration, and invasion of ... proliferation, migration, and invasion of 769P cells To examine the functions of PKCε, we knocked down PKCε by transfecting PKCε siRNA into 769P cells The mRNA and protein expression of PKCε was significantly...
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Báo cáo khoa học: "Production and purification of immunologically active core protein p24 from HIV-1 fused to ricin toxin B subunit in E. coli" ppt
Ngày tải lên :
12/08/2014, 04:21
... 6xHIS RTB p24 Figure of recombinant proteins expressed in E coli and immunodetection Purification Purification of recombinant proteins expressed in E coli and immunodetection Purification was performed ... band by three different antibodies targeting different components of the chimeric RTB/p24 protein, demonstrate the integrity of the protein after purification (Figure 3, panel C) Purification ... weight mass of the predicted translation products from sequences RTB/p24 and p24 was 57 kDa and 34 kDa respectively E coli expression of RTB/p24 and p24 proteins Purification of RTB/p24 and p24 was...
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Báo cáo khoa học: "Expression and characterization of the UL31 protein from duck enteritis virus" pot
Ngày tải lên :
12/08/2014, 04:21
... localization The sequence contains 28 possible sites for phosphorylation, 22 on serine, on threonine, and on tyrosine residues (Fig 1) Figure Prediction of Phosphorylation sites of deduced amid acids of ... genome containing the UL31 gene and strategy for construction of the Figure expression plasmid pET 32-UL31 A Schematic representation UL regions of the DEV genome containing the UL31 gene and strategy ... amplicon was cloned into a T/A Expression and purification of UL31-His fusion proteins The confirmed construct described above was used to chemically transform Escherichia coli BL21(DE3) for expression...
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Báo cáo y học: " Differential expression and function of breast regression protein 39 (BRP-39) in murine models of subacute cigarette smoke exposure and allergic airway inflammation" pps
Ngày tải lên :
12/08/2014, 13:22
... inflammation and expression of chitinases and chitinase-like molecules To investigate the impact of cigarette smoke exposure on chitinase expression, BALB/c, C57BL/6, and CD1 mice were exposed to cigarette ... Page of 12 after weeks of HDM exposure (Figure 1C), confirming previous reports [15,16,27,28] Localization of BRP-39 expression after cigarette smoke exposure To investigate the cellular source of ... induce pulmonary BRP-39 production in models of allergic airway inflammation [15,29] To investigate the role of IL-13 in the cigarette smoke mediated induction Figure Cigarette smoke and HDM induce...
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Báo cáo y học: "Relationship between the tissue-specificity of mouse gene expression and the evolutionary origin and function of the protein" ppsx
Ngày tải lên :
14/08/2014, 14:21
... tissue Figure Expression distributions of mouse proteins Expression distributions of mouse proteins (a) Expression pattern of all mapped proteins expressed in at least a single tissue (5,528 out of ... determination and protein annotation as described in Materials and methods A schematic description of the expression profile determination and protein annotation as described in Materials and methods ... that the connection between age and expression is spurious due to the misclassification of rapidly evolving genes and the connection between tissue expression and recent rate of evolution, Subramanian...
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EXPRESSION AND FUNCTION OF THE PRL FAMILY OF PROTEIN TYROSINE PHOSPHATASE
Ngày tải lên :
24/08/2014, 11:44
... PRL-1 and PRL-2 expression in cancer Figure Comparison between mRNA and protein expression levels PRL-1 (A) and PRL-2 (B) mRNA and protein expression levels were compared in a selection of 30 ... for PRL-2 mRNA expression Figure Moderate and diffuse expression of PRL-1 mRNA (A) and strong expression of PRL-2 mRNA (B) in the stratified epithelia and elastocollagenous stroma of the skin In ... function The combined results of these studies have expanded our current understanding of the expression and function of the PRL family of enzymes as well as of the role these important signaling...
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Cloning, expression and characterization of novel helicobacter pylori differentiating antigen heat shock protein 20
Ngày tải lên :
12/09/2015, 10:19
... residues of HSP20 RESULTS 4.1 Preparation of recombinant HSP20 (rHSP20) 85 4.1.1 Construction of rHSP20 expression vector 85 4.1.2 Expression and purification rHSP20 protein 87 4.2 Preparation and ... APPENDIX PUBLICATIONS LIST OF FIGURES TITLE OF FIGUES PAGE Figure 3.1 Physical map of expression vector pET16b (Novagen) 45 Figure 3.2 Diagrammatical representation of the construction of pET16hsp20 ... Figure 4.1 Construction and identification of hsp20 expression vector 85 Figure 4.2 DNA sequence of hsp20 and deduced amino acid sequence of Helicobacter pylori NCTC11637 HSP20 86 Figure 4.3 Expression...
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Cloning, expression and characterization of oxysterol binding protein homologue 7 (OSH7) in yeast saccharomyces cerevisiae
Ngày tải lên :
03/10/2015, 20:57
... amplification and purification 48 2.5.1.3 Digestion 48 2.5.1.4 Converting 5’-overhang to a blunt end 49 2.5.1.5 Gel purification 49 2.5.1.6 Ligation 50 2.5.1.7 Preparations of competent cells and transformation ... 2.5.2.3 Generation of pADNS-OSH7 construct 52 2.5.2.4 Generation of pGEX-4T-2-OSH7 construct 52 Deletion of OSH7 53 2.6.1 PCR amplification and purification 53 2.6.2 Transformation of kanMX4 into ... deletion 105 4.4 Loss of Osh7p function increases sterol esterification 107 4.5 Understanding of the nature of the interaction between Osh7p and Vps4p and future directions 108 4.6 Understanding...
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Expression and characterization of FAT1 and atrophin 1 proteins regulating planar cell polarity and MBD1 protein involved in lymphoma
Ngày tải lên :
05/10/2015, 22:31
... phenol-chloroform purification and ligation of Fat1 33 3.2.3 Digestion, Phenol Chloroform extraction and Ligation of Atrophin1 35 3.3 35 PROTEIN Expression And PurificatiON 3.3.1 Expression of Fat1 ... Fat1 and Atrophin1 43 4.2 Expression of Fat1 and Atrophin1 47 4.2.1 Expression of Fat1 47 4.2.2 Expression of Atrophin1 48 4.2.2.1 Final Expression 48 4.3 50 Purification of Fat1 4.3.1 Affinity purification ... Figure 4.7 Final expression of Atrophin in pET32A 50 Figure 4.8 Purification of Fat1 sing TALON resin 51 Figure 4.9 FPLC profile of Fat1 51 Figure 4.10 DLS and native gel profile of Fat1 53 Figure...
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iec 60079-15 electrical apparatus for explosive gas atmospheres - construction, test and marking of type of protection n electrical apparatus
Ngày tải lên :
25/12/2013, 10:42
... Oui Non 9.2 Fermetures spộciales Non Non Non Non Non 9.3 Trous pour fermetures spộciales Non Non Non Non Non 10 Dispositifs de verrouillage Non Non Non Non Non 11 Traversộes Oui Oui Oui Oui Non ... scellement Non Non Non Non Non 13 Composants Ex Non Non Non Non Non 14 Elộments de raccordement et logements de raccordement Non Non Non Non Non 15 Elộments de raccordement pour conducteurs de ... sectionnement Oui Oui Oui Non Non 19 Exigences supplộmentaires pour les coupe-circuits fusible Non Non Non Non Non 20 Exigences supplộmentaires pour les prises de courant Non Non Non Non Non 21...
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