... Diego) Plated cells were fixed with 4% paraformaldehyde (Sigma-Aldrich) for 10 minutes, permeabilised with 0.1% Triton X-100 (Sigma-Aldrich) for 10 minutes, blocked with 5% FBS for hour, all at ... Neurospheres were dissociated with Accutase™ and blocked with FcR blocking reagent (Miltenyi Biotec, Bergisch Gladbach, Germany) For stemness analysis, cells were stained with anti-CD133/2-allophycocyanin ... cells were then treated within 3–5 days with 0.1 g/ml 23 colcemid (Invitrogen) for 24 hours Metaphase-arrested cells were pelleted (180g for 10 minutes) and hypotonic-treated with 0.075 M potassium...
Ngày tải lên: 03/10/2015, 21:57
... order to gather their opinions and suggestions of CS There are three categories: their attitudes towards the program as a whole, their preference of the five specific strategies and their suggestions ... diary d Discussing your feelings with someone else a Asking for clarification or verification b Asking for correction a cooperating with peers b Cooperating with proficient users of the new language ... The two groups that we chose in this study were arranged in accordance with their level of English based on the results of their placement test To sum up, we tried to select two compatible groups...
Ngày tải lên: 07/11/2012, 14:54
A new approach to semantic and syntactic functions of English adjectives – A contrastive analysis with their Vietnamese equivalents
... English adjectives with their definitions right at the beginning as well as semantic and syntactic functions of adjs The writer discusses their classification according to their usage then considers ... be used with a possessive s The problems of the poor or poor peoples problem is not correct grammatically Some adjectives used without the as head of a noun phrase in paired structures with and ... between English and Vietnamese adjective in terms of their syntactic functions as well as their orders are pointed out Firstly, in terms of their functions, both English and Vietnamese, generally,...
Ngày tải lên: 10/04/2013, 14:46
THE 11TH FORM NON ENGLISH MAJORS’ LEVEL OF SATISFACTION WITH THEIR READING COMPREHENSION LESSONS AT PHAN BOI CHAU SPECIALIZED UP
... crucial to make our students satisfied with their lessons This is because students cannot be sufficiently motivated to learn without feeling satisfied with what they are provided for In teaching ... cognitive styles, teachers’ satisfaction with their teaching does not necessarily mean students’ satisfaction it seems that some of them are not satisfied with their learning Actually, satisfaction ... what extent are they satisfied with their reading comprehension lessons in general? I.4 Scope of the study - -3- As mentioned above, students’ satisfaction with their reading lessons depends on...
Ngày tải lên: 07/09/2013, 13:48
Why Do Drug Dealers Still Live with Their Moms
... rarely hang out with crack dealers So the answer to this question begins with finding someone who did live among the drug dealers and managed to walk away with the secrets of their trade Sudhir ... much money, why are they still living with their mothers?—is that, except for the top cats, they don’t make much money They had no choice but to live with their mothers For every big earner, there ... Drug Dealers Living with Their Moms It may be sad but not surprising to learn that experts like Snyder can be self-interested to the point of deceit But they cannot deceive on their own Journalists...
Ngày tải lên: 17/10/2013, 18:20
Tài liệu Lab 5.2.5 Managing IOS Images with TFTP pptx
... the copy tftp flash command in a later step However, it is possible to save a copy of that file with the command copy flash tftp If there is a possibility of ever having to revert to that software ... instructions in the Copy IOS to TFTP server section Step Start and configure the Cisco TFTP Server a Check with the instructor as to the IP address of the Cisco TFTP server 2-7 CCNA 2: Routers and Routing ... same file size as the file stored on the TFTP server If the file sizes are not identical, check with the instructor Step 12 Copy the IOS image from the TFTP server a Now that the IOS is backed...
Ngày tải lên: 11/12/2013, 14:15
Tài liệu Lab 5.2.5a Managing IOS Images with TFTP pdf
... the copy tftp flash command in a later step However, it is possible to save a copy of that file with the command copy flash tftp If there is a possibility of ever having to revert to that software ... TFTP section at the end of this exercise Step Start and configure the CIscoTFTP Server a Check with the instructor as to the IP address of the Cisco TFTP server 2-7 CCNA 2: Routers and Routing ... same file size as the file stored on the TFTP server If the file sizes are not identical, check with the instructor Step 12 Copy the IOS image from the TFTP server a Now that the IOS is backed...
Ngày tải lên: 21/12/2013, 19:15
Tài liệu Lab 5.2.5b Managing IOS Images with ROMmon and Xmodem docx
... "flash:c1700-y-mz.122-11.T.bin" cisco 1721 (MPC860P) processor (revision 0x100) with 29492K/3276K bytes of memory Processor board ID FOC06380F0T (479701011), with hardware revision 0000 MPC860P processor: part number ... "flash:c1700-y-mz.122-11.T.bin" cisco 1721 (MPC860P) processor (revision 0x100) with 29492K/3276K bytes of memory Processor board ID FOC06380F95 (3103823619), with hardware revision 0000 MPC860P processor: part number ... Systems, Inc Router>ena Router#configure terminal Enter configuration commands, one per line End with CNTL/Z Router(config)#config-register 0x0 Router(config)#exit Router# *Mar 00:29:21.023: %SYS-5-CONFIG_I:...
Ngày tải lên: 21/12/2013, 19:15
Tài liệu Lab 5.2.6b Managing IOS Images with ROMmon and Xmodem pptx
... "flash:c1700-y-mz.122-11.T.bin" cisco 1721 (MPC860P) processor (revision 0x100) with 29492K/3276K bytes of memory Processor board ID FOC06380F0T (479701011), with hardware revision 0000 MPC860P processor: part number ... "flash:c1700-y-mz.122-11.T.bin" cisco 1721 (MPC860P) processor (revision 0x100) with 29492K/3276K bytes of memory Processor board ID FOC06380F95 (3103823619), with hardware revision 0000 MPC860P processor: part number ... follows: Router>enable Router#configure terminal Enter configuration commands, one per line End with CNTL/Z Router(config)#config-register 0x0 Router(config)#exit Router# *Mar 00:29:21.023: %SYS-5-CONFIG_I:...
Ngày tải lên: 21/12/2013, 19:15
Báo cáo khoa học: Interaction of G-rich GT oligonucleotides with nuclearassociated eEF1A is correlated with their antiproliferative effect in haematopoietic human cancer cell lines potx
... supplemented with mm phenylmethanesulfonyl fluoride (PMSF), 0.01% NP40 and 0.05 mgÆmL)1 salmon sperm DNA, for h with gentle stirring The beads were washed twice with buffer C and twice with buffer C without ... elution was made with three volumes of buffer C without NP40 adjusted to 1.5 m NaCl, incubating for 10 with gentle stirring The beads were then washed with TEN 100 and stored at °C with 0.01% NaN3 ... activity of the G-rich oligomers was found to be associated with their ability to form stable G-quartetcontaining structures and with their binding to specific cellular proteins, most likely nucleolin...
Ngày tải lên: 16/03/2014, 13:20
Panorama: A Database System that Annotates Its Answers to Queries with their Properties potx
... are offered to users along with each answer issued Database systems that venture beyond the straightforward execution of queries and attempt to provide their users with additional information ... Meta-information Meta-relations are manipulated with their own product, selection and projection operations We define these operations and review their properties, and then describe how queries ... variables with meta-tuples outside a , and thus define views that are not expressible entirely within the scheme A Such views are avoided if F is modified so that all products are performed first, and their...
Ngày tải lên: 16/03/2014, 16:20
Integrating with their Feet: Cross-Border Lending at the German-Austrian Border pdf
... officer with the firm’s manager The bank receives a signal that reveals the firm’s type correctly with probability s, s ≤ However, it becomes more difficult for the banker to acquire and deal with ... relationship with, because this goes beyond the survey’s scope With only few exceptions, all firms have the possibility of contacting at least one bank which is located directly in their municipality ... by cooperative banks (“Genossenschaftsbanken”, usually “Raiffeisenbanken”) with 27 percent and private commercial banks with 12 percent (Bundesbank, 2007) Savings banks and cooperative banks have...
Ngày tải lên: 22/03/2014, 20:21
committee on physics of the universe, national research council. connecting quarks with the cosmos.. eleven science questions for the new century
... much at odds with observation as is proton 30 CONNECTING QUARKS WITH THE COSMOS decay For mathematical consistency, the strengths of the forces and the radiation rates associated with various ... scientific opportunities for advancing our understand- CONNECTING QUARKS WITH THE COSMOS ing of the universe and the laws that govern it, but not within the budgets of the three agencies as they stand ... quarks that compose neutrons and protons and their equally fundamental partners the leptons (the electron, the muon, and the tau particle, along with their three neutrino partners) They have constructed...
Ngày tải lên: 24/04/2014, 17:17
langford’s starting photography the guide to great images with digital or film
... comments and direction Picture credits: With thanks to the great guys at www.ablestock.com for their generous support in supplying the cover picture and the tutorial images for this text Copyright © ... Photography is a hands-on book for those photographers just starting their love affair with photography It equally suits shooters with entry and mid-priced level film and digital cameras, students ... capabilities of beginners with modest gear, such as compact or single lens reflex (SLR) cameras (preferably with manual controls), and the knowledge and skill provided within Taking photographs...
Ngày tải lên: 01/06/2014, 10:31
Báo cáo hóa học: " Research Article Three-Dimensional SPIHT Coding of Volume Images with Random Access and Resolution Scalability" doc
... performances of the previously defined random access with resolution scalability (3DSPIHT-RARS) are compared with the best up to date method without taking into account the specific properties available ... algorithms are quite similar for the MR images SPIHT-RARS outperforms JPEG2000 on the CT images, but JPEG2000 gives a lower bit rate for hyperspectral images It has to be noted that the original ... full spatial resolution with reduced spectral resolution In [17, 18], the authors report DATA DECORRELATION AND TREE STRUCTURE 3D anisotropic wavelet transform Hyperspectral images contain one image...
Ngày tải lên: 22/06/2014, 00:20
Báo cáo hóa học: " A Frequency Domain Approach to Registration of Aliased Images with Application to Super-resolution" docx
... aliased images We use a planar motion model When a series of images is taken in a short amount of time with only small camera motion between the images, we assume that the motion can be described with ... reconstructed images From Figures and 9, it can be seen that with our algorithm, the registration was very accurate Most aliasing has been removed in both reconstructed images In the images obtained with ... versus the number of images used The performance increases rapidly with the first six images, but the improvement is marginal beyond that In the rest of this paper, we will use four images as input...
Ngày tải lên: 22/06/2014, 23:20
Báo cáo hóa học: " Research Article An Attention-Driven Model for Grouping Similar Images with Image Retrieval Applications" pptx
... most salient ROIs within an image These ROIs, once extracted, are then indexed (based on their features) and clustered with other similar ROIs that may have appeared in other images This paper ... images they are searching for Clustering techniques can be used to organize visually similar images into groups and minimize the number of undesired images shown to the user (ii) Navigation with ... systems CBIR refers to the retrieval of images according to their content, as opposed to the use of keywords The purpose of a CBIR system is to retrieve all the images that are relevant to a user...
Ngày tải lên: 22/06/2014, 23:20
Báo cáo hóa học: " Impulsive Noise Suppression from Images with the Noise Exclusive Filter" docx
... more pleasing images even if noise density is very high The major improvement achieved by the proposed NEF has been demonstrated with the extensive simulations of the mentioned test images corrupted ... corrupted pixels within the corrupted image, as explained in Sections 2.1 and 2.2 (3) Start the iterative computation process of NEF and perform the following steps for each corrupted pixel within the ... the number of uncorrupted pixels that exist within the current window, W Perform the following steps if the number of the uncorrupted pixels that exist within the current W is else than zero (i)...
Ngày tải lên: 23/06/2014, 01:20