concentration by precipitation with polyethylene glycol

... Polydimethylsiloxane Polyethylene glycol Poly(ethylene glycol) diacrylate Polyethylene oxide Poly(methylphenylsiloxane) Propylene oxide Poly(propylene glycol) Poly(propylene glycol) diacrylate ... gas (1 atm), with just 15 % CO2 concentration together with other component gasses, predominantly N2 Z.-Z Yang et al., Capture and Utilization of Carbon Dioxide with Polyethylene Glycol, SpringerBriefs ... (2005) Polyethylene glycol and solutions of polyethylene glycol as green reaction media Green Chem 7(2):64–82 Donaldson ME, Draucker LC, Blasucci V et al (2009) Liquid–liquid equilibria of polyethylene...

Ngày tải lên: 29/05/2014, 23:43

... in the presence of polymer PEG 4000 by thermal decomposition of iron acetylacetonate The morphology of product can be tuned by the amount of PEG 4000 When the concentration of PEG 4000 is decreased, ... and refluxed for 30 The product was black The black powder was collected with a permanent magnet, then redispersed in ethanol by supersonic for 15 The washing process was repeated three times At ... Rigaku D/max 2550V diffractometer equip˚ ped with a Cu KR (1.5418 A) X-ray source The morphology and chemical composition of the products were examined by transmission electron microscopy (TEM,...

Ngày tải lên: 22/06/2014, 00:20

... Engineering Title: Branched Polyethylene Glycol for Protein Precipitation Supervisor: Professor Reginald B.H TAN Abstract The use of linear polyethylene glycol (PEG) for protein precipitation raises ... have already been approved by FDA 2.3 Protein Precipitation by PEG Reported since the 1950's [e.g Stocking, 1956], precipitation by PEG is a common method for protein concentration and coarse bioseparation ... it came with less protein precipitation and slower kinetics Even so, higher concentrations of branched PEGs could still achieve net viscosity reductions without adversely affecting the precipitation...

Ngày tải lên: 10/09/2015, 15:52

... Dispersity Index Polyethylene glycol Positron emission tomography Poly (lactic acid) Poly (lactic acid)-D-alpha-tocopheryl polyethylene glycol 1000 succinate Poly (lactic acid)-poly (ethylene glycol) ... wavelength by changing the size and composition of QDs is another advantage of QDs For instance, 21 CdSe QDs with a nm diameter emit green light with a wavelength of 550 nm, whereas larger CdSe QDs with ... poly(lactic-co-glycolic acid) (PLGA) and various biodegradable copolymers such as poly(lactic acid)-poly (ethylene glycol) (PLEA) copolymer and poly (lactide acid)-dα-tocopheryl polyethylene glycol 1000...

Ngày tải lên: 13/10/2015, 15:54

... site with a 100% efficacy No inconvenience associated with Prevadh® administration was experienced throughout the study It is possible to achieve firm grasp by wetting the intact surface with ... is completely covered with mesothelium cells, 2: Fifty percent of pleural surface is covered with mesothelium cells, 3: Less than 50% of the pleural surface is covered with mesothelium cells, ... is completely covered with mesothelium cells, 2: Fifty percent of pleural surface is covered with mesothelium cells, 3: Less than 50% of the pleural surface is covered with mesothelium cells,...

Ngày tải lên: 25/10/2012, 11:00

... 0.20% - Polyethylene glycol (PEG): tinh khiết, dạng bột mịn, tập đoàn Merck Schuchard, Đức 2.2 Phương pháp nghiên cứu 2.2.1 Phương pháp tạo màng [9]: Qui trình tạo màng: Polyethylene glycol (PEG) ... vô cấp thiết Mục tiêu nghiên cứu tạo nên màng mỏng bao gói từ tinh bột sắn có phối trộn polyethylene glycol (PEG) tác nhân tạo liên kết, làm mềm dẻo, không độc hại xác định yếu tố ảnh hƣởng ... tạo màng Để ứng dụng tốt thƣờng tinh ́ ̀ ̉ bột đƣợc trộn thêm phụ gia thực phẩm khác nhƣ polyethylene glycol (PEG) (tác nhân làm mềm dẻo) Nƣớc ta có nguồn nguyên liệu tinh bột phong phú Ở miền...

Ngày tải lên: 16/11/2012, 14:25

... 0.20% - Polyethylene glycol (PEG): tinh khiết, dạng bột mịn, tập đoàn Merck Schuchard, Đức 2.2 Phương pháp nghiên cứu 2.2.1 Phương pháp tạo màng [9]: Qui trình tạo màng: Polyethylene glycol (PEG) ... vô cấp thiết Mục tiêu nghiên cứu tạo nên màng mỏng bao gói từ tinh bột sắn có phối trộn polyethylene glycol (PEG) tác nhân tạo liên kết, làm mềm dẻo, không độc hại xác định yếu tố ảnh hƣởng ... tạo màng Để ứng dụng tốt thƣờng tinh ́ ̀ ̉ bột đƣợc trộn thêm phụ gia thực phẩm khác nhƣ polyethylene glycol (PEG) (tác nhân làm mềm dẻo) Nƣớc ta có nguồn nguyên liệu tinh bột phong phú Ở miền...

Ngày tải lên: 19/11/2012, 12:38

... interfere with binding of the mature part to BMPR-IA As the BMPR-IA binding site for BMP-2 partially overlaps with the area bound by the antagonist noggin [32], we attempted to verify these findings by ... of BMP-2 interferes with BMP-2 signalling 18 16 14 12 10 0 0.1 0.5 10 20 50 Ligand concentration [nM] Fig Activation of the Smad1 ⁄ ⁄ pathway by BMP2 and proBMP2 as measured by reporter gene assay ... contrast, the ECD of BMPR-II was not bound by proBMP-2 Furthermore, the free propeptide formed a non-covalent complex with mature BMP-2 in vitro, thereby blocking binding of BMP-2 to BMPR-II The...

Ngày tải lên: 18/02/2014, 06:20

... prepared by incubating VVA1 beads with 45 nm VVA2 at 37 °C for 30 After removal of the unbound VVA2 by washing with 50 mm Tris buffer, the linear oligomeric VVA2 (VVA1 beads) was incubated with various ... supernatant and pellet were analyzed by 10% SDS ⁄ PAGE and visualized by Coomassie Blue staining Hemolytic activity assay Human RBCs were prepared by washing three times with NaCl ⁄ Pi (137 mm NaCl, ... incubated with various amounts of VVA2 The reaction products were eluted with 0.5% SDS loading buffer, and the proteins in the eluents were analyzed by 10% SDS ⁄ PAGE and visualized by silver...

Ngày tải lên: 19/02/2014, 06:20

... were performed by measuring the residual FAS activity after enzyme preincubation with inhibitors for 20 at 37 °C Potency was determined by dose–response curves in which the range of concentrations ... 3T3-L1 adipocytes by Oil Red O assay Adipocytes were washed twice with cold Dulbecco’s phosphate buffer saline and fixed with 10% formaldehyde for h Then, cells were washed with 60% isopropanol ... < 0.05 in one-way ANOVA analysis b docked individually within the predicted binding site using the DOCK function within moe and compared with the docking modes predicted for C75 and Orli- stat...

Ngày tải lên: 06/03/2014, 09:22

... 2.5 mm pNPP and NaCl and KCl at the concentrations indicated in the figure legends, with or without 0.1 mm ATP, with or without 10 lm oligomycin, and with or without mm ouabain The control experiment ... that increasing the ATP concentration activated ATPase, whereas pNPPase was activated by low ATP concentrations but inactivated by high ATP concentrations Because ATP concentrations up to 0.3 ... that NaE1P formed by pNPP with Na+, as proposed by Yamazaki et al [22], and NaE1P formed by ATP with Na+ are spontaneously dephosphorylated Figure 9A shows that increasing the KCl concentration activated...

Ngày tải lên: 07/03/2014, 16:20

... subjective than in text Annotation overview The images were annotated with one of several labels by one of the authors out of context (without considering the web site and its text), after applying ... meta senses, without a clear trend clusters (Sch¨ tze, 1998), but may be due to the u spectral clustering Inspection showed that clusters were dominated by core senses, whereas with 40 clusters ... three ambiguous keywords inspired by (Yarowsky, 1995): BASS, CRANE, and SQUASH We apply query augmentation (cf Table 1), and exact duplicates were filtered out by identical image URLs, but cases...

Ngày tải lên: 08/03/2014, 02:21

... phosphorylation by PAI-1 NIH3T3 cells were incubated overnight with 100 nM echistatin (Ec) or decorsin (Dc), or for h with 30 nM VN, 100 nM PAI-1 or 200 nM uPA, prior to treatment with 170 nM insulin ... Matrigel was also induced by insulin and inhibited by PAI-1 (Fig 3C) indicating that the interference of PAI-1 with insulin-induced migration occurred through its interaction with VN/avb3, independently ... experiments performed activity or by interacting with VN [21,27] Our observation that PAI-1 can inhibit insulin-induced HUVEC migration and angiotube formation by interfering with the VN/avb3 system suggests...

Ngày tải lên: 08/03/2014, 08:20

... career development and counseling Within months of deciding to become a career counselor, I had appointments booked for months with a waiting list! I worked with clients in industries as diverse ... an audio system with six speakers, or a 5-year unconditional factory warranty? The “special features” on this car are like the selected skills you bring with you to the interview By mentioning ... 4, you’ll learn how you can adjust your competencies to match those most valued by the company by aligning them with the company mission and company culture Your Gift The last skill I would like...

Ngày tải lên: 16/03/2014, 08:41

... collected by centrifugation and washed three times with immunoprecipitation buffer For co-immunoprecipitation of the kinase with the SAFB proteins, extracts of HeLa cells transfected with equal ... with TNMT buffer The bound proteins were eluted in SDS sample buffer, analysed by SDS ⁄ PAGE and visualized by western blotting using the relevant antibody Immunoprecipitation For the immunoprecipitation ... of SRPK1a SRPK1/1a inhibition by interaction with SAFB1/2 FLAG–SRPK1 activity is inhibited in vitro by SAFB1 and SAFB2 We next asked whether the inhibition exerted by SAFB1 on SRPK1a activity is...

Ngày tải lên: 30/03/2014, 01:20

... open questions, initial trials with QA -by- Dossier -with- Constraints have been very encouraging, whether it is by correctly answering previously missed questions, or by improving confidences of correct ... discovered, and so on QA -by- Dossier -with- Constraints extends this approach by capitalizing on the fact that a set of answers about a subject must be mutually consistent, with respect to constraints ... used in TREC2003, in which collections of facts can be gathered by QA -by- Dossier, we can assume that QDC is always appropriate By defining broad enough classes of entities for which these questions...

Ngày tải lên: 31/03/2014, 03:20

... probed with PEP-specific antibody S449 (1 : 400) and anti-actin (1 : 2500) and detected by chemiluminescence Suppression of PEP expression in U343 cells A cell line with sufficiently high concentrations ... results obtained with the antisense cell lines, basal Ins(1,4,5)P3 concentration was increased in cells treated with PEP inhibitor (Fig 2) However, the observed change in Ins(1,4,5)P3 concentration ... Ins(1,4,5)P3 increase by prolyl endopeptidase (Eur J Biochem 269) 5817 Fig Ins(1,4,5)P3 concentrations in various U343 cell lines stimulated by Substance P Ins(1,4,5)P3 concentrations were measured...

Ngày tải lên: 31/03/2014, 08:20

... ATPase Figure 3D shows the inhibition of purified ATPase with varying concentrations of Mg2+ Mg2+ inhibits ATPase activity at high concentrations with an IC50 value of mM, which is not changed in the ... skeletal muscle, as described by Michelangeli & Munkonge [22] Cerebellar microsomes were prepared as described by Sayers et al [23] Microsomes from COS-7 cells transfected with SERCA cDNA were prepared ... range of ATP concentrations (specific activity 100 and 10 CiÆmol)1, respectively) The reaction was initiated by addition of the appropriate amounts of [c-32P]ATP and inactivated 15 s later by the addition...

Ngày tải lên: 31/03/2014, 23:20

... Optics OPTICS Learning by Computing, with Examples ® ® Using Mathcad , Matlab , Mathematica®, and Maple® Second Edition ¨ K.D Moller With 308 Illustrations Includes CD-ROM With Mathcad Matlab Mathematica ... 1.3 we show a crosssection of a prism with apex angle A and refractive index n The incident ray makes an angle θ1 with the normal, and the angle of deviation with respect to the incident light is ... magnification of each of the two lenses One has m m1 m2 with m1 xi1 /xo1 and m2 xi2 /xo2 , where m1 is calculated with respect to the first lens and m2 with respect to the second lens FileFig 1.15 (G15TINTOW)...

Ngày tải lên: 08/04/2014, 10:27

... lượng đạm: 0.20%  Polyethylene glycol (PEG): tinh khiết, dạng bột mịn, tập đoàn Merck Schuchard, Đức 2.2 PHƯƠNG PHÁP NGHIÊN CỨU 2.2.1 PHƯƠNG PHÁP TẠO MÀNG Tinh Polyethylene glycol (PEG) Nước ... cao, hạn chế Mục tiêu nghiên cứu tạo nên màng mỏng bao gói từ tinh bột sắn có phối trộn polyethylene glycol (PEG) tác nhân tạo liên kết, làm mềm dẻo, không độc hại xác định yếu tố ảnh hưởng ... nguyên Mạch polysaccharides Để ứng dụng tốt thường tinh bột trộn thêm phụ gia thực phẩm khác polyethylene glycol (PEG) (tác nhân làm mềm dẻo) Các polysaccharides tự nhiên: - Dễ phân hủy , đặc biệt...

Ngày tải lên: 04/05/2014, 22:15