coli expression of rtb p24 and p24 proteins
Báo cáo khoa học: "Deletions of neuraminidase and resistance to oseltamivir may be a consequence of restricted receptor specificity in recent H3N2 influenza viruses" doc
... enzyme If the only function of NA is to cleave receptors bound by the HA, then HA and NA activity of a viable virus should be matched quantitatively (Kd of HA and Kcat of NA) and qualitatively (same ... the viral glycoproteins (only Nlinked glycans) than from the cell surface (N-linked and O-linked glycans and glycolipids) Thus the specificities of HA and NA not need to be matched and, furthermore, ... Schematic showing examples of deletions of the NA coding region of A/OK/323/03 In all sequences, the 5' and 3' ends are retained Lines represent internal deletions and the number of nucleotides deleted...
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Báo cáo y học: " Contribution of the C-terminal tri-lysine regions of human immunodeficiency virus type 1 integrase for efficient reverse transcription and viral DNA nuclear import" pps
... 48 hours of infection, equal amounts of cells were lysed in 50 µl of luc lysis buffer and then, 10 µl of cell lysates was used for measurement of luc activity, as described in Materials and Methods ... respectively 70%, 72% and 68% of viral DNA associated with nuclear fractions (Fig 5C (upper panel, lanes and 2; and 4; and 10) and 5D) For KK240,4AE mutant, approximately 51% of viral DNA was nucleusassociated ... localization, we replaced an arginine and a lysine at positions of 263 and 264 by alanines in this region and generated a mutant (INRK263,4AA-YFP) The protein expression of different IN-YFP mutants in...
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Báo cáo sinh học: " Quantitative assessment of the effect of uracil-DNA glycosylase on amplicon DNA degradation and RNA amplification in reverse transcription-PCR" pdf
... real-time PCR and RTPCR offers significant advantages over standard PCR and RT-PCR for detection of DNA or RNA in terms of reduced sample handling, the time required for analysis and analytical ... elimination of carry-over contamination has led several manufacturers of commercial PCR and RT-PCR reagents to substitute dUTP in the reaction mixture in place of dTTP and in the case of some manufacturers ... Simultaneous detection of viral RNA and contaminating DNA Simultaneous detection of viral RNA and contaminating DNA Serial ten-fold dilutions of viral RNA with approximately 1,000 copies of heterologous...
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báo cáo hóa học:" Quantitative assessment of the effect of uracil-DNA glycosylase on amplicon DNA degradation and RNA amplification in reverse transcription-PCR" ppt
... real-time PCR and RTPCR offers significant advantages over standard PCR and RT-PCR for detection of DNA or RNA in terms of reduced sample handling, the time required for analysis and analytical ... elimination of carry-over contamination has led several manufacturers of commercial PCR and RT-PCR reagents to substitute dUTP in the reaction mixture in place of dTTP and in the case of some manufacturers ... Simultaneous detection of viral RNA and contaminating DNA Simultaneous detection of viral RNA and contaminating DNA Serial ten-fold dilutions of viral RNA with approximately 1,000 copies of heterologous...
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Báo cáo hóa học: " A broad spectrum, one-step reverse-transcription PCR amplification of the neuraminidase gene from multiple subtypes of influenza A virus" pdf
... cause of morbidity and mortality in humans and in a wide range of animal species [1-3] Type "A" viruses are the most important pathogens of the three types and have been associated with all of the ... of 1,101 fulllength NA sequences of the subtypes, of a range of host species and from different geographical locations were retrieved and aligned using Biological Sequence Alignment editor software ... evaluation of the one-step RT-PCR assay and sequencing for N subtyping of influenza at AAHL, helped to draft and edit the manuscript, and participated in the overview of the study RB conceived of the...
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báo cáo khoa học: " An optimized grapevine RNA isolation procedure and statistical determination of reference genes for real-time RT-PCR during berry development" docx
... β-tubulin, PP2A, and SAND, with Ct values of 26 or higher The calculated coefficient of variance of the Ct values gives an indication of the expression stability of a particular gene β-tubulin and cyclophilin ... Realtime RT-PCR is currently one of the more powerful and sensitive techniques for analyzing gene expression It provides outstanding accuracy of RNA quantification and has a broad dynamic range ... geNorm software [16]; and 3) deviations from the Ct mean [17] Lastly, we demonstrate the effects of using one or more reference genes on the relative expression levels of an aquaporin and a sucrose...
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Phát hiện và phân biệt chủng virus gây hội chứng rối loạn sinh sản và hô hấp ở lợn bằng phương pháp multiplex RT PCR (reverse transcription polymerase chain reaction)
... localization signal Vùng tín hiệu định vị hạch nhân Porcine Reproductive and Respiratory Syndrome Porcine Reproductive and Respiratory Syndrome Virus Hội chứng rối loạn sinh sản hô hấp lợn Virus ... 1.1.4 Đƣờng truyền lây virus 1.2 Tổng quan virus PRRS (Porcine reproductive and respiratory syndrome virus) 1.2.1 Đặc điểm virus 1.2.2 Cấu trúc virus ... QUAN TÀI LIỆU 1.1 Khái quát hội chứng hô hấp sinh sản lợn (PRRS - Porcine reproductive and respiratory syndrome) 1.1.1 Sự xuất phát sinh dịch bệnh bệnh PRRSV 1.1.2 Triệu...
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Specific and sensitive quantitative RT-PCR of miRNAs with DNA primers doc
... freedom of primer design and specificity of the LNA-based method Optimization of primer Tm and high specificity of the PCR reaction is achieved by adding a tail to each of the PCR primers Page of ... 100 ng of RNA in a final volume of 10 μl including μl of 10x poly(A) polymerase buffer, 0.1 mM of ATP, μM of RT-primer, 0.1 mM of each deoxynucleotide (dATP, dCTP, dGTP and dTTP), 100 units of MuLV ... determination of the quantification cycle (Cq) and PCR efficiency was calculated from the log-linear portion of the standard curves [17] Comparison of the efficiency of qPCR with LNAspiked and DNA...
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Báo cáo y học: "Investigation of infectious agents associated with arthritis by reverse transcription PCR of bacterial rRNA" ppsx
... samples and approximately half was then treated with DNase I, and then aliquots of both were subjected to RT-PCR using universal primers R1 and R2 for detection of bacterial 16S rRNA sequences Of ... these were tested against a panel of µl aliquots from 96 sequenced bacteria PCR products inserted into recombinant E coli Using standard reaction mixes and standard cycling times (see above), ... understanding of the pathogenesis of postinfectious arthritis Materials and method Patients SF samples were collected by routine aspiration, mainly from patients attending the Department of Medicine,...
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báo cáo khoa học: "Application of in situ reverse trancriptase-polymerase chain reaction (RT-PCR) to tissue microarrays" pot
... relative to a set of standards and expressed here as copy number of original transcripts per ng of mRNA Staecker et al [1], using fluorescence detection of amplified molecules Use of the technique ... RT-PCR methods, however, and this may be due to technical complexity and an inherent lack of reproducibility [2,3] The processing of large numbers of samples is also slow and sequential, adding ... analysis of expression of the extracellular matrix protein gene spondin [4] was carried out on a series of total RNA preparations from normal human tissue and paired normal/cancer samples Selection of...
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Báo cáo y học: " The RNA binding protein HuR does not interact directly with HIV-1 reverse transcriptase and does not affect reverse transcription in vitro" doc
... Figure Purification and biophysical characterization of GSTand NusA-HuR (A) Analysis of the expression and purification of GSTHuR by SDS PAGE Lanes and illustrate total protein and total soluble ... solution and the molecular masses of NusA-HuR and NusARRM were determined as 82.2 and 68.9 kDa, respectively These values are within ± 15% of the predicted masses of 99 kDa for NusA-HuR and 73 ... H domain of RT and to the C-terminus of HuR (see Fig 1) Importantly, siRNA knockdown of HuR expression in HeLa P4.2 cells was reported to greatly impair both the early and late steps of viral...
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Báo cáo y học: "Real-time reverse-transcription PCR in the diagnosis of influenza A (H1N1)v in intensive care unit adult patients" pdf
... Investigators: Critical care services and 2009 H1N1 influenza in Australia and New Zealand N Engl J Med 2009, 361 [Epub ahead of print] Page of (page number not for citation purposes) ... D: Evaluation of four real-time PCR assays for detection of influenza A(H1N1)v viruses Euro Surveill 2009, 14:pii 19230 The ANZIC Influenza Investigators: Critical care services and 2009 H1N1 ... RT-PCR for diagnosis of influenza A (H1N1)v pneumonia, which is important in the decision-making process Only 25% of our patients started antiviral treatment within 48 hours of influenza onset...
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DNA, RNA, replication, translation, and transcription
... in a daughter strand one strand from each of the initial two strands end up Each strand serves as a template for a new strand New strand is formed by complementary base-pairing of the correct nucleotide ... replicated on the surface of new copies of the phage • amplification in E coli and random mutagenesis to build a library of different phages encoding for different proteins • exposed to immobilized ... group) • uracil instead of thymine (loss of a methyl group) • single-stranded, and typically folds into unique shapes, like proteins • less chemically stable Other kinds of RNA • Ribosomal RNA,...
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Báo cáo khoa học: Ribonuclease H: properties, substrate specificity and roles in retroviral reverse transcription ppt
... SW, Goff S & Baltimore D (1979) A detailed model of reverse transcription and tests of crucial aspects Cell 18, 93–100 Telesnitsky A & Goff SP (1997) Reverse transcriptase and the generation of ... The RNase H domains of M-MLV and HIV-1 reverse transcriptases are structurally very similar to the Escherichia coli and Bacillus halodurans RNases H, and to human RNase H1, and these similarities ... which one strand is entirely DNA and the other strand is RNA at the 5¢-end followed by a stretch of DNA, the HIV-1 and M-MLV retroviral RNases H strongly prefer to cleave the RNA strand one nucleotide...
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Báo cáo hóa học: "Rapid detection of sacbrood virus (SBV) by one-step reverse transcription loop-mediated isothermal amplification assay" pptx
... contributions JY, YR and KS carried out most of the experiments and wrote the manuscript, and should be considered as first authors XP and ZL critically revised the manuscript and the experiment ... of SBV Figure Determination of the optimal temperature of the LAMP Determination of the optimal temperature Lane M, DL-2000 DNA marker; Lanes 1–7: LAMP carried out at 59, 60, 61, 62, 63, 64 and ... time of between 30 and 50 at a temperature in the range 63°C–65°C Figure Determination of the optimal time of the LAMP Lane M, DL-2000 DNA marker; Lanes 1–5: LAMP carried out for 10, 20, 30, 40 and...
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Báo cáo khoa học: "Development of a Lightcycler-based reverse transcription polymerase chain reaction for the detection of foot-and-mouth disease virus" pps
... demrofrep erew noitceted tcudorp dna noitacifilpmA elcyc hcae rof ces/C 02 fo pmar eht htiw nim rof C 06 ta pets noisnetxe/gnilaenna dna ces rof C 49 ta pets noitarutaned fo selcyc 55 yb dewollof ... eht rof ylno detcelfer saw noiger B2 laitrap ot redaeL morf gnirevoc ,revewoh ,niarts 55/lizarb/oriezurc/42A rof ecneuqes ehT sniarts 21 eht rof dezylana saw noiger tegrat ehT )ASU ,ratsanD( ... elbaliava gnisu dengised erew stegrat lla rof seborp dna sremirp RCP-TR* emaN ecneuqeS eborP esreveR drawroF eborP esreveR drawroF eborP esreveR drawroF '3-pxGCCAATTTCAGCCCAGGCACAAAm-'5 '3-TYCGGTTYGGGACCATGTT-'5...
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Báo cáo khoa hoc:" Quantitative competitive reverse transcription polymerase chain reaction is not a useful method for quantification of CD4 and CD8 cell status during HIV infection" pptx
... Buffer (Gibco BRL, Grand Island, NY), 0.25 µg of each primer, mM MgC12 in each CD4 reaction and mM MgC12 for CD8 reactions, mM total dNTP's, and 0.5 U of Taq Polymerase For CD4 and CD8 reaction conditions ... translation or surface expression of CD8, although it is not used as a viral receptor Whatever the mechanism, no correlation between CD4 and CD8 cell counts and mRNA levels of CD4 and CD8 could be ... -0.2S and 0.13 respectively http://www.jnrbm.com/content/2/1/2 structural protein expression [4] These and other mechanisms may explain the lack of correlation between message and surface expression...
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Báo cáo y học: "Comparative study between the Hybrid Capture II test and PCR based assay for the detection of human papillomavirus DNA in oral submucous fibrosis and oral squamous cell carcinoma" pdf
... value (RLU of specimen/ mean RLU of PC) was 1.0 pg/ml of sample Interpretation of Cut off value (RLU of specimen/mean RLU of PC) Cut-off ratio of to 0.99 is negative for HR HPV; Cutoff ratio greater ... mediated by expression of the viral E6 and E7 oncoproteins, which cause deregulation of the cell cycle by inactivating p53 and pRb respectively [9] Integration often disrupts the integrity and expression ... off ratio at 1.0 correspond to viral DNA load of 5,000 copies/ml of 1pg/ml at a threshold of finding a clinical disease or prognosis of an OSMF and OSCC lesions DNA extraction and diagnosis of...
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Báo cáo y học: " Variability in a dominant block to SIV early reverse transcription in rhesus monkey cells predicts in vivo viral replication and time to death" pot
... The ability of a cell to become infected by HIV/SIV requires cellular expression of diverse proteins and HIV/SIV replication can be repressed by the cellular expression of a number of restriction ... manipulated by insertion of GFP ORF into the env gene of SIV543 (SIVsm543-GFP) to allow the expression of GFP following SIV integration, transcription and translation of viral proteins This construct ... number/cell of each viral DNA species and the SIVsmE660 permissivity of that cell A significant positive correlation was observed between copy number of early RT, late RT, and integrated copy number and...
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Báo cáo y học: " A multiplex reverse transcription-nested polymerase chain reaction for detection and differentiation of wild-type and vaccine strains of canine distemper virus" pptx
... specific and sensitive assay for the rapid detection and differentiation of wildtype and vaccine strains of CDV For lack of other vaccine strains except CDV Onderstepoort strain, the primers P5 and ... products were cloned and sequenced, and the sequences were aligned with the H genes of a number of CDV strains available in GenBank using the MegAlign function of the DNAStar software package Thirty ... of the immunofluorescence assay with RT-PCR and nested PCR in the diagnosis of canine distemper Vet Res Commun 2005, 29:347-59 10 Li JZ, He HB, Xia XZ, et al.: Establishment and application of...
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Tìm thêm: hệ việt nam nhật bản và sức hấp dẫn của tiếng nhật tại việt nam xác định các nguyên tắc biên soạn khảo sát các chuẩn giảng dạy tiếng nhật từ góc độ lí thuyết và thực tiễn khảo sát chương trình đào tạo của các đơn vị đào tạo tại nhật bản xác định thời lượng học về mặt lí thuyết và thực tế tiến hành xây dựng chương trình đào tạo dành cho đối tượng không chuyên ngữ tại việt nam điều tra đối với đối tượng giảng viên và đối tượng quản lí điều tra với đối tượng sinh viên học tiếng nhật không chuyên ngữ1 khảo sát các chương trình đào tạo theo những bộ giáo trình tiêu biểu nội dung cụ thể cho từng kĩ năng ở từng cấp độ phát huy những thành tựu công nghệ mới nhất được áp dụng vào công tác dạy và học ngoại ngữ mở máy động cơ rôto dây quấn các đặc tính của động cơ điện không đồng bộ hệ số công suất cosp fi p2 đặc tuyến tốc độ rôto n fi p2 đặc tuyến dòng điện stato i1 fi p2 động cơ điện không đồng bộ một pha sự cần thiết phải đầu tư xây dựng nhà máy thông tin liên lạc và các dịch vụ chỉ tiêu chất lượng 9 tr 25