coli based rtb p24 induced strong immune responses against p24 hiv
Báo cáo khoa học: "Deletions of neuraminidase and resistance to oseltamivir may be a consequence of restricted receptor specificity in recent H3N2 influenza viruses" doc
... glycoproteins (only Nlinked glycans) than from the cell surface (N-linked and O-linked glycans and glycolipids) Thus the specificities of HA and NA not need to be matched and, furthermore, NA is not ... for shorter NA segments, with little or no requirement for NA activity, or as negative selection against NA activity but a requirement for at least the 3' and 5' NA RNA sequences The total yield...
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Báo cáo y học: " Contribution of the C-terminal tri-lysine regions of human immunodeficiency virus type 1 integrase for efficient reverse transcription and viral DNA nuclear import" pps
... human anti -HIV serum Then, immunoprecipitates were run in 12% SDS-PAGE and analyzed by Western blot with rabbit anti-IN antibody and anti -p24 monoclonal antibody or anti -HIV serum HIV- 1 reverse-transcribed ... to be a component of HIV PIC [28,29] However, whether this IN/LEDGF/p75 interaction plays an important role for HIV- 1 nuclear import still remains to be elucidated, since HIV- 1 infection and replication ... contributing to its karyophilic property A HIV- 1 IN-YFP fusion protein expressor (CMV-IN-YFP) was generated by fusing a full-length HIV- 1 IN cDNA (amplified from HIV1 HxBru molecular clone [30]) to...
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Báo cáo sinh học: " Quantitative assessment of the effect of uracil-DNA glycosylase on amplicon DNA degradation and RNA amplification in reverse transcription-PCR" pdf
... amplicon DNA while only slightly increasing the CT for RNA detection It is interesting to note that, based on analysis of the standard curves, increases observed in the CT for RNA were due both to ... modified nucleotides and uracil-DNA glycosylase (UNG) for the control of contamination in the PCR -based amplification of RNA Mol Cell Probes 1992, 6:251-256 Rys PN, Persing DH: Preventing false ... biological warfare and infectious disease agents Biotechniques 2003, 35:862-869 Yang S, Rothman RE: PCR -based diagnostics for infectious diseases: uses, limitations, and future applications in acute-care...
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báo cáo hóa học:" Quantitative assessment of the effect of uracil-DNA glycosylase on amplicon DNA degradation and RNA amplification in reverse transcription-PCR" ppt
... amplicon DNA while only slightly increasing the CT for RNA detection It is interesting to note that, based on analysis of the standard curves, increases observed in the CT for RNA were due both to ... modified nucleotides and uracil-DNA glycosylase (UNG) for the control of contamination in the PCR -based amplification of RNA Mol Cell Probes 1992, 6:251-256 Rys PN, Persing DH: Preventing false ... biological warfare and infectious disease agents Biotechniques 2003, 35:862-869 Yang S, Rothman RE: PCR -based diagnostics for infectious diseases: uses, limitations, and future applications in acute-care...
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Báo cáo hóa học: " A broad spectrum, one-step reverse-transcription PCR amplification of the neuraminidase gene from multiple subtypes of influenza A virus" pdf
... negative sense RNA viruses of the family Orthomyxoviridae and are classified into types (A, B or C) based on the antigenic difference in their nucleoproteins (NP) and matrix proteins (M1) Influenza ... use of potentially effective antiviral treatment [14,19,20] To date, influenza diagnostic methods based on reverse transcription-PCR (RT-PCR) and real-time RT-PCR (RRT-PCR) are currently available ... have designed a primer set for a one-step RT-PCR detection assay of multiple influenza A viruses, based on the detection of the NA gene Our one-step RT-PCR method, followed by sequencing, was validated...
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báo cáo khoa học: " An optimized grapevine RNA isolation procedure and statistical determination of reference genes for real-time RT-PCR during berry development" docx
... date, a limited number of real-time RT-PCR experiments focusing on grape berries has been published Based on microarray and real-time RT-PCR data, UBQ-L40 [14] and one paralog of EF1-α [2] were previously ... When assessing a set of reference genes, the evaluation method implemented can be a source of bias based on the assumptions underlying each approach In an effort to Page of 11 (page number not for ... reference genes [16] This program calculates a gene expression stability measure (M) for each gene based on the average pairwise expression ratio between it and each of the other genes being studied...
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Specific and sensitive quantitative RT-PCR of miRNAs with DNA primers doc
... unspecific amplicon will be indistinguishable from the desired PCR product The recently published method based on circularization of the miRNA also depends on a specific primer for reverse transcription ... this method has all the benefits regarding freedom of primer design and specificity of the LNA -based method Optimization of primer Tm and high specificity of the PCR reaction is achieved by adding ... microRNA LNA™ PCR primer sets designed by Exiqon (Denmark) qPCR data analysis Quantification was based on determination of the quantification cycle (Cq) and PCR efficiency was calculated from the...
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Báo cáo y học: "Investigation of infectious agents associated with arthritis by reverse transcription PCR of bacterial rRNA" ppsx
... patients (including the E coli sequences), arguing against laboratory contamination Together, this constitutes strong evidence of the ability of commensal organisms such as E coli to colonize inflamed ... vigorous T-cell mediated immune responses, as suggested by the consistent finding of prominent responses to triggering organisms in ReA SF T cells [23,24] In contrast, the immune system is usually ... specific immune responses, they may have a proinflammatory effect because of the effects of component such as lipopolysaccharide, bacterial DNA and bacterial heat shock proteins on the innate immune...
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báo cáo khoa học: "Application of in situ reverse trancriptase-polymerase chain reaction (RT-PCR) to tissue microarrays" pot
... tissues has certain advantages over more established hybridisation, PCR amplification or antibodybased techniques As with immunohistochemistry, detection of gene expression is at the level of individual...
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Báo cáo y học: " The RNA binding protein HuR does not interact directly with HIV-1 reverse transcriptase and does not affect reverse transcription in vitro" doc
... Steady-state DNA synthesis by HIV- 1 RT in the presence of NusA-HuR (A) HIV- 1 RT DNA synthesis on a heteropolymeric RNA template corresponding to the HIV- 1 sequence of (-) strong stop DNA The 18 nucleotide ... with full-length HIV- 1 RT (Fig 3A); (ii) the 15N-labeled RRM 1&2 of HuR was titrated with the RNase H domain of HIV- 1 RT (Fig 3B); and (iii) the 15N-labeled RNase H domain of HIV- 1 RT was titrated ... of HuR on HIV- 1 RT DNA synthesis, even on T/P substrates that contain AREs and bound both HIV- 1 RT and HuR (Fig 4B) Therefore, our results suggest that HuR does not interfere with HIV- 1 replication...
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DNA, RNA, replication, translation, and transcription
... so that the protein is replicated on the surface of new copies of the phage • amplification in E coli and random mutagenesis to build a library of different phages encoding for different proteins ... molecule (e.g., protein or DNA) © M S Shell 2009 11/12 last modified 10/27/2010 • wash • re-infect E coli to amplify DNA sequence • repeated rounds involving different levels of mutagenesis will sort ... using column chromatography uses the properties of solid matrix (beads) to selectively separate based on specific properties: • size – gel filtration • charge – ion exchange • hydrophobicity •...
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Báo cáo khoa học: Ribonuclease H: properties, substrate specificity and roles in retroviral reverse transcription ppt
... is present in the M-MLV, human and E coli RNases H, but absent in the RNases H from HIV- 1 and B halodurans [19,23– 30] Structure–function studies with the E coli and M-MLV RNases H implicate the ... has been extended [20–22] The RNase H domains of M-MLV and HIV- 1 reverse transcriptases are structurally very similar to the Escherichia coli and Bacillus halodurans RNases H, and to human RNase ... absence of a C-helix in the RNase H domain of HIV- 1 reverse transcriptase, modeling studies comparing the C-helix of M-MLV RNase H with the p66 subunit of the HIV- 1 enzyme suggest that a series of positively...
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Báo cáo hóa học: "Rapid detection of sacbrood virus (SBV) by one-step reverse transcription loop-mediated isothermal amplification assay" pptx
... isothermal amplification (RT-LAMP) does not require expensive or special equipment [3,4] Therefore, LAMP -based detection assays would be suitable for on-the-spot detection in the field or primitive laboratories...
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Báo cáo khoa hoc:" Quantitative competitive reverse transcription polymerase chain reaction is not a useful method for quantification of CD4 and CD8 cell status during HIV infection" pptx
... CD4 is not complete and the HIV- infection does not reduce CD4 mRNA levels [5] Thus, a method based on quantitating CD4 and CD8 mRNA could provide a means of assessing immune status without requiring ... mRNA as determined by QC-RT-PCR (A) CD4, and (B) CD8 The HIV status of each sample is depicted represents HIV positive status, ■ represents HIV negative status, and ▲ ! reepresents unknown status ... exquisitely sensitive This method may also be useful in assessing the immune status of patients retrospectively from archived or frozen samples QC-RT-PCR, and related methodologies, however,...
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Báo cáo y học: "Comparative study between the Hybrid Capture II test and PCR based assay for the detection of human papillomavirus DNA in oral submucous fibrosis and oral squamous cell carcinoma" pdf
... chemiluminiscent detection Procedure of the HC-II test First, double stranded DNA was denatured by using a strong alkaline denaturation solution and converted into single stranded DNA (ssDNA) This ssDNA ... There findings warrant further study with a larger number of patients Evidence has showed that strong epidemiology data would provide additional support for a causal association between HR-HPV ... Hahn LJ, Hsieh LL, Chen CJ: Interaction of collagen-related genes and susceptibility to betel quid -induced oral submucous fibrosis Cancer Epidemiol Biomarkers Prev 2002, 11(7):646-53 Mehrotra R,...
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Báo cáo y học: " Variability in a dominant block to SIV early reverse transcription in rhesus monkey cells predicts in vivo viral replication and time to death" pot
... of HIV- 1 to human leukocyte antigen class I Nature 2009, 458(7238):641-5 Liu R, et al.: Homozygous defect in HIV- 1 coreceptor accounts for resistance of some multiply-exposed individuals to HIV- 1 ... permissivity for SIV or HIV (Fig 4C, D, E, F, G) This study suggests that the relative block to SIVsmE660 infection in B-LCL acts through a mechanism that can also restrict other SIVs and HIV, but allows ... of HIV- infected humans and SIV-infected monkeys is likely a consequence of both host and viral factors The present study was initiated to begin an exploration of the impact of the intrinsic immune...
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Báo cáo y học: " A multiplex reverse transcription-nested polymerase chain reaction for detection and differentiation of wild-type and vaccine strains of canine distemper virus" pptx
... http://www.virologyj.com/content/7/1/86 Page of Results Phylogenetic analysis based on H gene Determination of the application conditions of the multiplex RT-nPCR A phylogenetic tree based on the H genes of various CDV strains ... a 2% (w/v) agarose gel Study Design With the help of Oligo6 software, six primers were designed based on the genomic sequences of CDV strains published in GenBank (CDV strains: Onderstepoort, ... constituting a genotype different from that of CDV vaccine strains, as revealed by phylogenetic analysis based on the sequences of the H gene region, which is believed to be the most reliable classification...
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Tìm thêm: hệ việt nam nhật bản và sức hấp dẫn của tiếng nhật tại việt nam xác định các mục tiêu của chương trình xác định các nguyên tắc biên soạn khảo sát chương trình đào tạo gắn với các giáo trình cụ thể xác định thời lượng học về mặt lí thuyết và thực tế điều tra đối với đối tượng giảng viên và đối tượng quản lí nội dung cụ thể cho từng kĩ năng ở từng cấp độ xác định mức độ đáp ứng về văn hoá và chuyên môn trong ct mở máy động cơ rôto dây quấn hệ số công suất cosp fi p2 đặc tuyến mômen quay m fi p2 đặc tuyến tốc độ rôto n fi p2 đặc tuyến dòng điện stato i1 fi p2 động cơ điện không đồng bộ một pha sự cần thiết phải đầu tư xây dựng nhà máy thông tin liên lạc và các dịch vụ phần 3 giới thiệu nguyên liệu từ bảng 3 1 ta thấy ngoài hai thành phần chủ yếu và chiếm tỷ lệ cao nhất là tinh bột và cacbonhydrat trong hạt gạo tẻ còn chứa đường cellulose hemicellulose chỉ tiêu chất lượng theo chất lượng phẩm chất sản phẩm khô từ gạo của bộ y tế năm 2008 chỉ tiêu chất lượng 9 tr 25