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Tài liệu Báo cáo khoa học: Structural insights into the substrate specificity and activity of ervatamins, the papain-like cysteine proteases from a tropical plant, Ervatamia coronaria ppt
Ngày tải lên :
18/02/2014, 16:20
... investigation
of the catalytic mechanism and substrate speci city
and to understand the differences therein for this class
of enzymes. In order to identify the subsites of the
ervatamins and to understand ... both the molecules of the
asymmetric unit runs along the extended backbone of
residues 65–64 and is partly exposed to the solvent
(Fig. 2B). On the other hand, the P3 moiety of E-64 in
each of the ... using the programs cns [58] and detwin and
sfcheck in the CCP4 suite [59] confirmed that the data
were twinned, and allowed us to calculate the twinning
fraction (0.394) (Fig. 6A,B) and the twinning...
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Báo cáo khoa học: Substrate specificity and transport mode of the proton-dependent amino acid transporter mPAT2 potx
Ngày tải lên :
07/03/2014, 16:20
... internal membrane side is
the rate-limiting s tep and not – as proposed for most of the
other electrogenic symporters – the return of the unloaded
transporter to the outside of the membrane [17].
In ... alanine, serine
and proline but also osmolytes such as sarcosine and
betaine, and the
D
-enantiomers of serine and alanine. The
apparent affinities of PAT1 substrates are mainly in the
range of 2–15 ... The apparent K
m
values of
D
-Pro,
D
-Ala and OH-Pro increased only 1.5- and 2.1-fold by
depolarizing the membrane from )120 to )20 mV. On the
other hand, the affinity of b-Ala did not change significantly
within...
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Tài liệu Cisco Press CCIE Practical Studies CCIE Practice Lab: “The lab, the bad, the ugly Solutions” ppt
Ngày tải lên :
18/01/2014, 05:20
... Changes and Interpretations
With any complex lab, there might be various interpretations of the meaning of the requirement.
Interpreting the problem statement is part of the difficulty of the lab. ... ambiguities, and the like. For this lab, the following lists the
interpretations used when compiling these answers:
Minor changes for clarity and function:
Section II: 1. Assign the management ...
chapter 18, and the solutions are not in the book. As promised in the book, the solutions are
posted are
www.ciscopress.com. This file contains the solutions to one of those labs.
The solutions...
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Tài liệu Báo cáo khoa học: Substrate specificity and inhibition of brassinin hydrolases, detoxifying enzymes from the plant pathogens Leptosphaeria maculans and Alternaria brassicicola ppt
Ngày tải lên :
18/02/2014, 14:20
... resulted in 55% and 51%
loss of the initial activities of BHLmL2 and BHAb,
respectively. These results strongly suggest that Ser is
involved in the catalytic activity of BHLmL2 and
BHAb and are consistent ... respectively.
The influence of pH on the activities of the BH
enzymes was investigated in the pH range 6–11. The
pH optima were determined to be in the basic range
(pH 8.0–10.0) for BHLmL2 and BHAb ... 6 and
Fig. 4, the Hill equation provided an excellent fit, with
coefficients of determination of 0.992 for BHLmL2
and 0.997 for BHAb. In addition, the fits of the
V = f(S) curves were sigmoidal and...
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Tài liệu Báo cáo khoa học: A knowledge-based potential function predicts the specificity and relative binding energy of RNA-binding proteins ppt
Ngày tải lên :
18/02/2014, 16:20
... evaluated by the statistical potential
used in the present study. In the case of the RNA muta-
tions to the SRP complex, the mutated RNA residues
are located in a double-stranded region of RNA, and do
not ... onto the RNA bases nearest the center of
the b-sheet structure of the RRM. We then scored
these sequence-variant structures with the distance-
dependent potential function.
Figure 2 shows the ... score; the blue line represents the rank of these
sequences using the distance-dependent potential. The points in
each colored line are sorted independently by rank; the x-axis is the
sort order. The...
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Tài liệu Báo cáo khoa học: The role in the substrate specificity and catalysis of residues forming the substrate aglycone-binding site of a b-glycosidase docx
Ngày tải lên :
18/02/2014, 17:20
... binding of the
Sfbgly interactions with the aglycone. Thus, the effect
of the alteration of the glycone structure in the stabil-
ity of the ES
à
complex (DDG
à
) for the wild-type and
mutant ... another important issue in understand-
ing the aglycone speci city is the contribution of the
interactions with the aglycone to the catalysis in
b-glycosidases. Mutational studies with ZmGlu1 and
SbDhr1 ... is that the
interactions with aglycone do not affect the binding of
the glycone within the Sfbgly active site.
This hypothesis was further investigated by deter-
mining the influence on the glycone...
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Báo cáo khoa học: Relation between domain evolution, specificity, and taxonomy of the a-amylase family members containing a C-terminal starch-binding domain pot
Ngày tải lên :
08/03/2014, 08:20
... 3B). Thus for the SBD tree there is not an
obvious border between the hydrolases and transglycosi-
dases, but rather there may be one between the compact
cluster of Bacillus and Thermoanaerobacter ... 251 CGTase with the SBDs
from the CGTases from Thermococcus sp. B1001, Bacillus
ohbensis,andThermoanaerobacter thermosulfurogenes are
37.3%, 63.8% and 74.0%, respectively. Even for the
acarviose ... supports the idea
that there has been a separate evolution of this domain [30].
This together with the findings of the present study indicates
a separate evolution of the domains C and E compared to
the...
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Begun and held at the City of Washington on Wednesday, the twenty-third day of January, two thousand and two potx
Ngày tải lên :
15/03/2014, 22:20
... consideration the size and
nature of the business of the accounting firms they supervise and
the size and nature of the business of the clients of those firms.
The standards applied by the Board under ... Act and the rules of the Board and
the Commission issued under this Act, in the same manner
and to the same extent as a public accounting firm that is
organized and operates under the laws of the ... reports and the obligations
and liabilities of accountants with respect thereto, by registered
public accounting firms and associated persons thereof; and
(7) set the budget and manage the operations...
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Báo cáo khoa học: Hybrid reuteransucrase enzymes reveal regions important for glucosidic linkage specificity and the transglucosylation / hydrolysis ratio pptx
Ngày tải lên :
16/03/2014, 04:20
... synthesis.
Conclusions
The N-termini of the catalytic domains of the GTFA
and GTFO reuteransucrases are the main determinants
for glucosidic linkage speci city. Within these N-ter-
mini, the ... a)
8
barrel with the three catalytic resi-
dues (Fig. 1C), determine the types and ratios of glu-
cosidic linkages synthesized (Table 1).
The A1
⁄
O1 and A2
⁄
O2 regions within the
N-termini of the catalytic ... sequencing),
both p15-GTFA-dN-RS and p15-GTFO-dN-RS were
digested with XbaI and SalI, SalI and SacI, and SacI and
KpnI, and corresponding fragments were exchanged, yielding
the six constructs p15-GTFA-O1-dN-RS,...
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Báo cáo khoa học: Kinetic studies and molecular modelling attribute a crucial role in the specificity and stereoselectivity of penicillin acylase to the pair ArgA145-ArgB263 pdf
Ngày tải lên :
16/03/2014, 16:20
... speci city is more
pronounced and the synthetic capacity is, respectively,
increased. In the latter system PA behaves as a typical
transferase [14,15]. The nucleophile or S
1
 [16] specicity of
the ... close to the position found in the
complex with PG [19]. The bond lengths and valence angles
of the nitroaniline moiety were taken from the X-ray data
for the system cyclophilin A and the tripeptide ... described [26].
The other substrates were obtained in dry organic
solvents in the presence of organic base (pyridine,
N-methylmorpholine). The solvents used for the synthesis
of the respective...
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Báo cáo Y học: The role of hydrophobic active-site residues in substrate specificity and acyl transfer activity of penicillin acylase pdf
Ngày tải lên :
17/03/2014, 23:20
... may
influence the shape and volume of the acyl binding pocket
and thereby alter the binding mode and affinity of the
enzyme for PAA and derivatives thereof, while maintaining
the hydrophobicity of the binding ... site.
To test the effect of the mutations on the speci city for
phenylacetylated substrates, the steady-state kinetic param-
eters for the hydrolysis of the chromogenic substrate
NIPAB and the inhibition ... AD and P refer to the acyl donor and
product, respectively. The speci city (k
cat
/K
m
)ofPAforthe
produced antibiotic is in general 10-fold higher than the
speci city for the corresponding acyl...
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Báo cáo " ENVIRONMENTAL EDUCATION THROUGH HUMAN EDUCATION – METHODS OF TEACHING PEOPLE RAISE ENVIRONMENTAL PROTECT AWARENESS – SOME ENVIRONMENTAL EDUCATION AND COMMUNICATION ACTIVITIES IN THE HO CHI MINH CITY UNIVERSITY OF TECHNOLOGY " pdf
Ngày tải lên :
22/03/2014, 09:20
... cheap price, but other aspects of tax evasion, thus harming the common interests of
society and harm the industry. Vedan still the same. On the other hand they produce for the market
to provide ... music is the kind of culture
contributes to enrich the soul of every man and guide them to the lofty goals in life.
Each child brings in his part of the material taken from the family and partly ... We still talk to each other that when we do wrong, other
people may not know, but heaven knows, that land, and another thing to know, that is the human
conscience. So the human conscience is...
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Begun and held at the City of Washington on Tuesday, the sixth day of January, two thousand and nine docx
Ngày tải lên :
23/03/2014, 10:20
... after the enactment of
this Act, the Comptroller General of the United States shall report
to the Congress on the actions of the Administrator in implementing
the authorities established in the ... may consider the following fac-
tors:
‘‘(1) The viability of the project without guarantees.
‘‘(2) The availability of other Federal and State incentives.
‘‘(3) The importance of the project ... at the
end the following:
‘‘(9) Notwithstanding any other provision of law or any
rule, regulation, or order of the Administration, for purposes
H. R.1—45
of sections 410, 411, and 412 the...
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Begun and held at the City of Washington on Tuesday, the sixth day of January, two thousand and nine potx
Ngày tải lên :
29/03/2014, 06:21
... unless the consumer,
before making such election, received a notice from the creditor
of any over -the- limit fee in the form and manner, and at the
time, determined by the Board. If the consumer ... or the
consumer reduces the outstanding balance below the credit
limit as of the end of such billing cycle.
H. R.627—8
(B) in the table of sections for the chapter, by adding
at the end the ... the creditor
disclosed to the consumer, in a clear and conspicuous
manner, the length of the period and the annual percentage
rate that would apply after expiration of the period;
‘‘(B) the...
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Báo cáo khoa học: Characterization of recombinant prolidase from Lactococcus lactis – changes in substrate specificity by metal cations, and allosteric behavior of the peptidase pdf
Ngày tải lên :
30/03/2014, 04:20
... prolidase. The plots
show the relationship between the observed activity and the con-
centration of Leu-Pro (open squares) and Arg-Pro (closed circles).
The inset shows the Hill plot of the assay ... USA). The size of the columns
was 0.5 diameter · 10 cm, and 20 mm sodium citrate buffer
(pH 6.5) ⁄ 1mm ZnCl
2
was used as the eluant. The eluate
was fractionated, and the activities of the fractions ... discussed the contribution of this loop struc-
ture as part of the substrate binding site, and it was
suggested that Leu37B (B indicates the contribution
from the other subunit) formed the hydrophobic...
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