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Tài liệu Báo cáo khoa học: Structural insights into the substrate specificity and activity of ervatamins, the papain-like cysteine proteases from a tropical plant, Ervatamia coronaria ppt
... investigation of the catalytic mechanism and substrate speci city and to understand the differences therein for this class of enzymes. In order to identify the subsites of the ervatamins and to understand ... both the molecules of the asymmetric unit runs along the extended backbone of residues 65–64 and is partly exposed to the solvent (Fig. 2B). On the other hand, the P3 moiety of E-64 in each of the ... using the programs cns [58] and detwin and sfcheck in the CCP4 suite [59] confirmed that the data were twinned, and allowed us to calculate the twinning fraction (0.394) (Fig. 6A,B) and the twinning...
Ngày tải lên: 18/02/2014, 16:20
Báo cáo khoa học: Substrate specificity and transport mode of the proton-dependent amino acid transporter mPAT2 potx
... internal membrane side is the rate-limiting s tep and not – as proposed for most of the other electrogenic symporters – the return of the unloaded transporter to the outside of the membrane [17]. In ... alanine, serine and proline but also osmolytes such as sarcosine and betaine, and the D -enantiomers of serine and alanine. The apparent affinities of PAT1 substrates are mainly in the range of 2–15 ... The apparent K m values of D -Pro, D -Ala and OH-Pro increased only 1.5- and 2.1-fold by depolarizing the membrane from )120 to )20 mV. On the other hand, the affinity of b-Ala did not change significantly within...
Ngày tải lên: 07/03/2014, 16:20
Báo cáo sinh học: "Dishevelled and Wnt signaling: is the nucleus the final frontier" potx
Ngày tải lên: 06/08/2014, 18:21
Tài liệu Cisco Press CCIE Practical Studies CCIE Practice Lab: “The lab, the bad, the ugly Solutions” ppt
... Changes and Interpretations With any complex lab, there might be various interpretations of the meaning of the requirement. Interpreting the problem statement is part of the difficulty of the lab. ... ambiguities, and the like. For this lab, the following lists the interpretations used when compiling these answers: Minor changes for clarity and function: Section II: 1. Assign the management ... chapter 18, and the solutions are not in the book. As promised in the book, the solutions are posted are www.ciscopress.com. This file contains the solutions to one of those labs. The solutions...
Ngày tải lên: 18/01/2014, 05:20
Ngoài thể rắn, thể lỏng, thể khí ra vật chất còn ở trạng thái nào khác nữa không pptx
Ngày tải lên: 22/07/2014, 00:21
Tài liệu Báo cáo khoa học: Substrate specificity and inhibition of brassinin hydrolases, detoxifying enzymes from the plant pathogens Leptosphaeria maculans and Alternaria brassicicola ppt
... resulted in 55% and 51% loss of the initial activities of BHLmL2 and BHAb, respectively. These results strongly suggest that Ser is involved in the catalytic activity of BHLmL2 and BHAb and are consistent ... respectively. The influence of pH on the activities of the BH enzymes was investigated in the pH range 6–11. The pH optima were determined to be in the basic range (pH 8.0–10.0) for BHLmL2 and BHAb ... 6 and Fig. 4, the Hill equation provided an excellent fit, with coefficients of determination of 0.992 for BHLmL2 and 0.997 for BHAb. In addition, the fits of the V = f(S) curves were sigmoidal and...
Ngày tải lên: 18/02/2014, 14:20
Tài liệu Báo cáo khoa học: A knowledge-based potential function predicts the specificity and relative binding energy of RNA-binding proteins ppt
... evaluated by the statistical potential used in the present study. In the case of the RNA muta- tions to the SRP complex, the mutated RNA residues are located in a double-stranded region of RNA, and do not ... onto the RNA bases nearest the center of the b-sheet structure of the RRM. We then scored these sequence-variant structures with the distance- dependent potential function. Figure 2 shows the ... score; the blue line represents the rank of these sequences using the distance-dependent potential. The points in each colored line are sorted independently by rank; the x-axis is the sort order. The...
Ngày tải lên: 18/02/2014, 16:20
Tài liệu Báo cáo khoa học: The role in the substrate specificity and catalysis of residues forming the substrate aglycone-binding site of a b-glycosidase docx
... binding of the Sfbgly interactions with the aglycone. Thus, the effect of the alteration of the glycone structure in the stabil- ity of the ES à complex (DDG à ) for the wild-type and mutant ... another important issue in understand- ing the aglycone speci city is the contribution of the interactions with the aglycone to the catalysis in b-glycosidases. Mutational studies with ZmGlu1 and SbDhr1 ... is that the interactions with aglycone do not affect the binding of the glycone within the Sfbgly active site. This hypothesis was further investigated by deter- mining the influence on the glycone...
Ngày tải lên: 18/02/2014, 17:20
Báo cáo khoa học: Relation between domain evolution, specificity, and taxonomy of the a-amylase family members containing a C-terminal starch-binding domain pot
... 3B). Thus for the SBD tree there is not an obvious border between the hydrolases and transglycosi- dases, but rather there may be one between the compact cluster of Bacillus and Thermoanaerobacter ... 251 CGTase with the SBDs from the CGTases from Thermococcus sp. B1001, Bacillus ohbensis,andThermoanaerobacter thermosulfurogenes are 37.3%, 63.8% and 74.0%, respectively. Even for the acarviose ... supports the idea that there has been a separate evolution of this domain [30]. This together with the findings of the present study indicates a separate evolution of the domains C and E compared to the...
Ngày tải lên: 08/03/2014, 08:20
Begun and held at the City of Washington on Wednesday, the twenty-third day of January, two thousand and two potx
... consideration the size and nature of the business of the accounting firms they supervise and the size and nature of the business of the clients of those firms. The standards applied by the Board under ... Act and the rules of the Board and the Commission issued under this Act, in the same manner and to the same extent as a public accounting firm that is organized and operates under the laws of the ... reports and the obligations and liabilities of accountants with respect thereto, by registered public accounting firms and associated persons thereof; and (7) set the budget and manage the operations...
Ngày tải lên: 15/03/2014, 22:20
Báo cáo khoa học: Hybrid reuteransucrase enzymes reveal regions important for glucosidic linkage specificity and the transglucosylation / hydrolysis ratio pptx
... synthesis. Conclusions The N-termini of the catalytic domains of the GTFA and GTFO reuteransucrases are the main determinants for glucosidic linkage speci city. Within these N-ter- mini, the ... a) 8 barrel with the three catalytic resi- dues (Fig. 1C), determine the types and ratios of glu- cosidic linkages synthesized (Table 1). The A1 ⁄ O1 and A2 ⁄ O2 regions within the N-termini of the catalytic ... sequencing), both p15-GTFA-dN-RS and p15-GTFO-dN-RS were digested with XbaI and SalI, SalI and SacI, and SacI and KpnI, and corresponding fragments were exchanged, yielding the six constructs p15-GTFA-O1-dN-RS,...
Ngày tải lên: 16/03/2014, 04:20
Báo cáo khoa học: Kinetic studies and molecular modelling attribute a crucial role in the specificity and stereoselectivity of penicillin acylase to the pair ArgA145-ArgB263 pdf
... speci city is more pronounced and the synthetic capacity is, respectively, increased. In the latter system PA behaves as a typical transferase [14,15]. The nucleophile or S 1 Â [16] specicity of the ... close to the position found in the complex with PG [19]. The bond lengths and valence angles of the nitroaniline moiety were taken from the X-ray data for the system cyclophilin A and the tripeptide ... described [26]. The other substrates were obtained in dry organic solvents in the presence of organic base (pyridine, N-methylmorpholine). The solvents used for the synthesis of the respective...
Ngày tải lên: 16/03/2014, 16:20
Báo cáo Y học: The role of hydrophobic active-site residues in substrate specificity and acyl transfer activity of penicillin acylase pdf
... may influence the shape and volume of the acyl binding pocket and thereby alter the binding mode and affinity of the enzyme for PAA and derivatives thereof, while maintaining the hydrophobicity of the binding ... site. To test the effect of the mutations on the speci city for phenylacetylated substrates, the steady-state kinetic param- eters for the hydrolysis of the chromogenic substrate NIPAB and the inhibition ... AD and P refer to the acyl donor and product, respectively. The speci city (k cat /K m )ofPAforthe produced antibiotic is in general 10-fold higher than the speci city for the corresponding acyl...
Ngày tải lên: 17/03/2014, 23:20
Báo cáo " ENVIRONMENTAL EDUCATION THROUGH HUMAN EDUCATION – METHODS OF TEACHING PEOPLE RAISE ENVIRONMENTAL PROTECT AWARENESS – SOME ENVIRONMENTAL EDUCATION AND COMMUNICATION ACTIVITIES IN THE HO CHI MINH CITY UNIVERSITY OF TECHNOLOGY " pdf
... cheap price, but other aspects of tax evasion, thus harming the common interests of society and harm the industry. Vedan still the same. On the other hand they produce for the market to provide ... music is the kind of culture contributes to enrich the soul of every man and guide them to the lofty goals in life. Each child brings in his part of the material taken from the family and partly ... We still talk to each other that when we do wrong, other people may not know, but heaven knows, that land, and another thing to know, that is the human conscience. So the human conscience is...
Ngày tải lên: 22/03/2014, 09:20
Begun and held at the City of Washington on Tuesday, the sixth day of January, two thousand and nine docx
... after the enactment of this Act, the Comptroller General of the United States shall report to the Congress on the actions of the Administrator in implementing the authorities established in the ... may consider the following fac- tors: ‘‘(1) The viability of the project without guarantees. ‘‘(2) The availability of other Federal and State incentives. ‘‘(3) The importance of the project ... at the end the following: ‘‘(9) Notwithstanding any other provision of law or any rule, regulation, or order of the Administration, for purposes H. R.1—45 of sections 410, 411, and 412 the...
Ngày tải lên: 23/03/2014, 10:20
Begun and held at the City of Washington on Tuesday, the sixth day of January, two thousand and nine potx
... unless the consumer, before making such election, received a notice from the creditor of any over -the- limit fee in the form and manner, and at the time, determined by the Board. If the consumer ... or the consumer reduces the outstanding balance below the credit limit as of the end of such billing cycle. H. R.627—8 (B) in the table of sections for the chapter, by adding at the end the ... the creditor disclosed to the consumer, in a clear and conspicuous manner, the length of the period and the annual percentage rate that would apply after expiration of the period; ‘‘(B) the...
Ngày tải lên: 29/03/2014, 06:21
Báo cáo khoa học: Characterization of recombinant prolidase from Lactococcus lactis – changes in substrate specificity by metal cations, and allosteric behavior of the peptidase pdf
... prolidase. The plots show the relationship between the observed activity and the con- centration of Leu-Pro (open squares) and Arg-Pro (closed circles). The inset shows the Hill plot of the assay ... USA). The size of the columns was 0.5 diameter · 10 cm, and 20 mm sodium citrate buffer (pH 6.5) ⁄ 1mm ZnCl 2 was used as the eluant. The eluate was fractionated, and the activities of the fractions ... discussed the contribution of this loop struc- ture as part of the substrate binding site, and it was suggested that Leu37B (B indicates the contribution from the other subunit) formed the hydrophobic...
Ngày tải lên: 30/03/2014, 04:20
