chromatography fourier transform infrared spectroscopy tlc ftir and nuclear magnetic resonance tlc nmr

High-Performance Thin-Layer Chromatography(HPTLC)

Ngày tải lên : 06/10/2016, 09:26

... direct on-line coupled HPTLC FTIR offers advantages relative to other hyphenated techniques (HPTLC–Raman spectroscopy, HPTLC–PA, and HPTLC–MS), such as: the ease of operation and the optimized operational ... successfully hyphenated with high-performance liquid chromatography (HPLC), mass spectroscopy (MS), Fourier transform infra-red (FTIR) , and Raman spectroscopy to give far more detailed analytical ... quantification, analytical analysis, finger print analysis; and HPTLC future to combinatorial approach, potential for hyphenation, HPTLC–MS, HPTLC FTIR, and HPTLC–scanning diode laser The chapters in the book...

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báo cáo khoa học: " Integration of molecular biology tools for identifying promoters and genes abundantly expressed in flowers of Oncidium Gower Ramsey" pps

Ngày tải lên : 11/08/2014, 11:21

... filters were 458 nm/band-pass 465-510 nm for CFP, 488 nm/bandpass 500-530 nm for GFP, 514 nm/ bandpass 525-555 nm for YFP, 561 nm/bandpass 575-630 nm for RFP and mCherry, and 488 nm/band-pass 650-710 ... (Invitrogen), and into p2YGWF (cGFP) and p2GWF7 (N-YFP) using Gateway LR Clonase II Enzyme Mix (Invitrogen) [44] The plasmids were isolated and transformed into the lips using bombardment transformation ... binding sites and tandem repeats were analyzed using the Plant Promoter Analysis Navigator [46] Acknowledgements We thank Dr Yu-Yun Chang for helpful discussions and Dr Heiko Kuhn and Ms Miranda Loney...

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Tài liệu Báo cáo khóa học: Active-site residues and amino acid speciﬁcity of the bacterial 4¢-phosphopantothenoylcysteine synthetase CoaB pptx

Ngày tải lên : 19/02/2014, 12:20

... and 450 nm Molecular mass information was obtained as described [6], determining the elution volumes of standard proteins and the void volume of the column The Superdex 200 PC 3.2/30 column and ... puriﬁed by reversed phase chromatography and then analyzed by MALDI-TOF mass spectrometry In the mass spectrum two prominent peaks with m/z values of 604.7 and 323.1 were present and are proposed to ... (forward) 5¢-GCCAGTTTTGAATTCTGGAAAGCGC CTCG-3¢ and (reverse) 5¢-CGGGTCCAAGATCTTAA CGTCGATTTTTTTC-3¢ as primers (introduced EcoRI and BglII sites are underlined) and the puriﬁed chromosomal DNA as template...

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Tài liệu Báo cáo khoa học: The role of the SEA (sea urchin sperm protein, enterokinase and agrin) module in cleavage of membrane-tethered mucins pdf

Ngày tải lên : 19/02/2014, 18:20

... consists of three a-helices and six b-strands forming an a ⁄ b sandwich fold The FRPG ⁄ SVVV residues of the MUC1 cleavage site, which is located in a loop between b2 and b3 strands, are illustrated ... MUC3- and MUC12- cleavage sites and the MUC3 and MUC12 SEA domains All constructs were based on the MUC1FDTR backbone [18,13], and are illustrated in Fig Using unique PsiI (J05582: 3351) and DraIII ... 818–841) and 12-SEA-R 5¢-CCGCT CGAGTAGCACAGTATGGCCTTTC-3¢ (AF147790 1153– 1171) SpeI and XhoI sites were incorporated at the 5¢ end of the forward and reverse primers, respectively, and MUC3 and MUC12-speciﬁc...

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Child-friendly health care: the views and experiences of children and young people in Council of Europe member States doc

Ngày tải lên : 17/03/2014, 17:20

... Bosnia and Herzegovina, Bulgaria, Estonia, Finland, France, Georgia, Germany, Greece, Ireland, Italy, Malta, Netherlands, Poland, Portugal, Romania, Serbia, Slovakia, Slovenia, Spain and the ... logistical and methodological – and it is important that the Council of Europe and other organisations continue to learn from, and improve, their work in this field How the bodies who ask children and ... care and fulfil commitments under the United Nations Convention on the Rights of the Child (UNCRC) and other international standards At its meeting in December 2010, the Committee of Experts mandated...

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Báo cáo khoa học: Mechanism for transcriptional synergy between interferon regulatory factor (IRF)-3 and IRF-7 in activation of the interferon-b gene promoter docx

Ngày tải lên : 23/03/2014, 13:20

... 6%) and –C2 ( 6%) (but weak binding to CBP-C, 1%), and to p300–N ( 11%), –C ( 14%), –C1 ( 7%) and –C2 ( 27%) Synergy between ATF-2/c-Jun and IRF-3/IRF-7 The afﬁnity of ATF-2/c-Jun and ... to 1.5% of input and binding to other GST–p300/ CBP fusions did not exceed 0.5% of input By contrast, p65 bound strongly to the N-, C1- and C2-regions of p300, and to the N- and C2-regions of ... promoter in virus-infected cells can be expressed and activate transcription in insect cells, and the p300 and CBP coactivators have distinct and speciﬁc effects on their transcriptional activity...

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Báo cáo khoa học: Subcellular localization of yeast Sec14 homologues and their involvement in regulation of phospholipid turnover pptx

Ngày tải lên : 23/03/2014, 18:20

... select additional steroids and triterpenoids: gravity-ﬂow column liquid chromatography, thin-layer chromatography, gas-liquid chromatography and high-performance liquid chromatography J Chromatogr ... lacking inositol and choline (Ins–/Cho–) or supplemented with 75 lM inositol and mM choline (Ins+/Cho+) were prepared as described previously [16] Plasmid and strain construction Standard genetic ... DNA and cloned into the high-copy number plasmid YEplac181 [19] Using primers F(SFH1-EGFP) and R(SFH1-EGFP) (Table 4) a 2663 bp SFH1-EGFP PCR fragment was generated, cut with PstI and BglI and...

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báo cáo hóa học:" Sigma-2 receptor ligands potentiate conventional chemotherapies and improve survival in models of pancreatic adenocarcinoma" ppt

Ngày tải lên : 18/06/2014, 15:20

... receptor ligands Sigma-2 specific ligands SV119, SV95, and fluorescent labeled sigma-2 ligand, SW120, were synthesized and prepared as previously described [13-15] The Sigma-1 receptor ligand, (+)-pentazocine ... resistance to many standard cancer treatments [12,18-21] Standard therapies including most chemotherapeutics and radiation therapy induce cellular stress and thereby promote apoptosis Standard therapies ... receptors exist: sigma-1 and sigma-2 subtype [5] These subtypes display different tissue distributions and distinct physiological and pharmacological profiles in both the central and peripheral nervous...

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báo cáo hóa học: " Severe depression is associated with increased microglial quinolinic acid in subregions of the anterior cingulate gyrus: Evidence for an immune-modulated glutamatergic neurotransmission?" doc

Ngày tải lên : 19/06/2014, 22:20

... BB and AMM have designed the study CM has done the routine neuropathological examination DSM-IV axis I diagnosis of MDD and BD was established in consensus meetings of JS and HB JS, TG, HGB and ... TG, GJG, LMS and AMM analyzed the data and interpreted the results RB was involved in the creation of figures JS, MW, TG, ZS, BB and AMM wrote the manuscript All authors have read and approved ... depression: an in vivo proton magnetic resonance spectroscopy study Biol Psychiatry 2000, 47:305-313 50 Rosenberg DR, Macmaster FP, Mirza Y, Smith JM, Easter PC, Banerjee SP, Bhandari R, Boyd C, Lynch...

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báo cáo hóa học: " Proinflammatory and proapoptotic markers in relation to mono and di-cations in plasma of autistic patients from Saudi Arabia" docx

Ngày tải lên : 19/06/2014, 22:20

... Mean with the standard error bars of measured Caspase3, IL6 and TNFa (a), Ca2+, Mg2+ and Ca2+/Mg2+(b), and Na+, K+ and Na+/K+(c) in autistic patients (N = 25) compared to age and gender matching ... Caspase3 and IL6 (positive correlation); (b): Caspase3 and TNFa (positive correlation); (c): Caspase3 and Ca2+ (positive correlation); (d): Caspase3 and K+ (negative correlation); (e): Caspase3 and ... brain injury but develop neurodevelopmental and motor disabilities Table and Figures and show the unexpected lower concentrations of caspase3, TNFa and IL6 This could be interpreted on the basis...

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báo cáo hóa học:" The role of FGF-2 and BMP-2 in regulation of gene induction, cell proliferation and mineralization" pptx

Ngày tải lên : 20/06/2014, 04:20

... S: Evaluation of fetal bone structure and mineralization in IGF-I deficient mice using synchrotron radiation microtomography and Fourier transform infrared spectroscopy Bone 2007, 40:160-168 34 ... profile of igf-1, alp, and bmp-2 and significantly increased expression of other genes associated with mineralization like col1a1, fn, ilgf-1, noggin and oc Fgf-2, on the other hand, significantly ... a feed-forward loop Although Figures and demonstrate the relative FGF-2 regulation and sequential expression of growth, angiogenic and chemotactic genes and depresses expression of mineralization-related...

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báo cáo hóa học:" The role of FGF-2 and BMP-2 in regulation of gene induction, cell proliferation and mineralization" pdf

Ngày tải lên : 20/06/2014, 07:20

... S: Evaluation of fetal bone structure and mineralization in IGF-I deficient mice using synchrotron radiation microtomography and Fourier transform infrared spectroscopy Bone 2007, 40:160-168 34 ... profile of igf-1, alp, and bmp-2 and significantly increased expression of other genes associated with mineralization like col1a1, fn, ilgf-1, noggin and oc Fgf-2, on the other hand, significantly ... a feed-forward loop Although Figures and demonstrate the relative FGF-2 regulation and sequential expression of growth, angiogenic and chemotactic genes and depresses expression of mineralization-related...

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Báo cáo hóa học: " A survey of classical methods and new trends in pansharpening of multispectral images" docx

Ngày tải lên : 20/06/2014, 22:20

... + μb , (1) σPAN where μPAN and μb are the mean of the PAN and MS image band b, respectively, and s PAN and s b are the standard deviation of the PAN and MS image band b, respectively This technique ... Forward transform the PAN and MS images using a sub-band and directional decomposition such as the subsampled or non-subsampled wavelet or contourlet transform Apply a fusion rule onto the transform ... color composites [14] The ability of IHS transform to Page of 22 separate effectively spatial information (band I) and spectral information (bands H and S) [20] makes it very applicable in pan-sharpening...

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Báo cáo lâm nghiệp: "Moisture effect on carbon and nitrogen mineralization in topsoil of Changbai Mountain, Northeast China" pptx

Ngày tải lên : 07/08/2014, 10:21

... influence of elevation and associated vegetation type and the effect of moistureelevation interaction on C0 and C0k indicated that the environmental effect on C0 and C0k was complex and deserves further ... alter microbial conditions and ultimately affect C0 and C0k According to the effect on k and C0k, we divided the elevation levels into groups 800 m, 1,600–1,800 m and 2,000 m, which was similar ... Ulmus propinqua, Fraxinus mandshurica, Abies holophylla and Betula costata; the dominant shrub species are Corylus mandshurica, Philadelphus schrenkii, Deutzia amurensis and Eleutherococcus senticosus;...

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Báo cáo lâm nghiệp: "Infra-red images of heat field around a linear heater and sap flow in stems of lime trees under natural and experimental conditions" doc

Ngày tải lên : 08/08/2014, 01:22

... theoretical understanding of the relation between measurable parameters of the heat field and sap flow and for methodical aspects of sap flow techniques MATERIALS AND METHODS 2.1 Site and sample trees ... Tenerife, Canary Islands, Tree Physiol 20 (2000) 1149-1156 [12] Lambs L., Muller E., Sap flow and water transfer in the Garrone River riparian woodland, France: first results on poplar and willow, Ann ... immediately after the cut at the observed stem side and changed it to 3.3.2.1 Cutting (step 1) and removing (step 2) the branch above the sensor (Fig 2, left and middle panels) Abrupt decrease of flow...

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Báo cáo lâm nghiệp: "Starch and soluble carbohydrates in leaves of water-stressed oak saplings" ppsx

Ngày tải lên : 08/08/2014, 18:21

... sphagnum peat and sandy soil in a naturally illuminated greenhouse They were fertilised four times each year with a complete nutrient solution and irrigated twice per week (see Epron and Dreyer, ... into glucose and fructose by an invertase (E.C 3.2.1.26) Glucose and fructose were phosphorylated to glucose-6-P and fructose-6-P using an hexokinase (E.C 2.7.1.1).Fructose-6-P was transformed ... Enzymes and buffer media were provided by Boehringer Mannheim RESULTS AND DISCUSSION either under controlled (Epron and Dreyer, 1990; Epron et al, 1993) or under natural conditions (Epron and Dreyer,...

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Báo cáo lâm nghiệp: " A flow cytometric evaluation of the nuclear DNA content and GC percent in genomes of European oak species" pptx

Ngày tải lên : 08/08/2014, 18:21

... petraea 2C 1.6 pg (Band, 1984 in Bennett and Smith, 1991), 1.8 pg (Greilhuber, 1988) or 1.58 pg (Ohri and Ahuja, 1990), Q robur 2C= 1.59 pg (Ohri and Ahuja, 1990), 1.61 pg (Ohri and Ahuja, Q rubra ... base-specific dye and applying the fifth root relationship of Godelle et al (1993): RESULTS AND DISCUSSION Results are given in tableI They show a relatively uniform nuclear DNA content and base composition ... to avoid doublets, as explained in a general review of the method by Marie and Brown (1993) Nuclear DNA content values and GC% were calculated from five to six different samples of 000-6 000 isolated...

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Báo cáo khoa học: "Drought susceptibility and xylem dysfunction in seedlings of 4 European oak species" pdf

Ngày tải lên : 08/08/2014, 19:21

... applied between the sensor and the bark to improve acoustic contact Linear differential variable transformers (LVDTs) were mounted in metal frames (Higgs and Jones, 1984) and used to continuously ... differbetween Q robur, Q petraea and Q cerris in the relationship between g and Ψ s A similar relationship was obtained between s g and pre-dawn Ψ in adult Q petraea and Q robur, with no species ... techniques to be introduced and, eventually, provide strategies for breeding superior and rugged trees that are able to withstand such stresses potential, stomatal conductance and cavitation on a sapling...

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Báo cáo khoa học: "Temporal and spatial variation in transpiration of Norway spruce stands within a forested catchment of the Fichtelgebirge, Germany" pdf

Ngày tải lên : 09/08/2014, 04:20

... at 35 and 90 cm deep at the 40-year stand, the 40-year boggy stand and the 140-year stand, and with manually recorded tensiometers at 20 cm deep at the 40-year NE stand, the 70-year stand and the ... gat the 40-year stand, the 40-1 year boggy stand and the 40-year NE stand, respectively) than in older stands (4.97 ± 0.52 mg g and 5.53 ± 0.45 mg -1 -1 gat the 70-year stand and the 140-year ... 40-concentrations 2+ year boggy stand and the 110-year stand are significantly different (P < 0.05) from the Mg of the 40-year-concentrations 2+ stand, the 40-year NE stand and the 140year stand 1-year-old Differences...

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money macroeconomics and keynes essays in honour of victoria chick volume 1 phần 1 docx

Ngày tải lên : 09/08/2014, 19:22

... stream from France and Italy: the Circuit approach 91 JOSEPH HALEVI AND RÉDOUANE TAOUIL 11 IS–LM and macroeconomics after Keynes 103 PETER KRIESLER AND JOHN NEVILE 12 On Keynes and Chick on prices ... demand and supply diagram in r, Y space 15.2 Alternative presentation of aggregate demand and supply in r, Y space 15.3 Adjustment to a lower real rate of interest 15.4 Effect of effective demand ... gold and siluer, whych are gathered from the Indies and other countries, and so yearely transported vnto these costes (Tawney and Power 1953, II: 310) This shift in emphasis between the 1549 and...

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