chondrogenic differentiation of bone marrow derived mesenchymal stem cells tips and tricks
Differentiation of bone marrow derived mesenchymal stem cells (BM MSCs) using engineered nanofiber substrates
Ngày tải lên :
11/09/2015, 09:57
... Types of cells used in bone tissue engineering for osteogenic differentiation 59 2.3.1 Potential of mesenchymal stem cells (MSCs) for bone healing 60 2.3.2 Potential of bone marrow derived mesenchymal ... cells: MSCs, osteoprogenitor cells, osteoblasts, chondrocytes Mitogenic for mesenchymal cells and osteoblasts, chemotactic for inflammatory and mesenchymal cells Targeted cells: mesenchymal and ... inflammatory cells, osteoblasts Differentiation of undifferentiated mesenchymal cells into chondrocytes and osteoblasts and osteoprogenitor cells into osteoblasts Targeted cells: mesenchymal and osteoprogenitor...
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Augmentation of neovascularization in murine hindlimb ischemia by combined therapy with simvastatin and bone marrow-derived mesenchymal stem cells transplantation pptx
Ngày tải lên :
10/08/2014, 05:21
... Page of 10 Figure Characterization of bone marrow- derived MSCs Flow cytometric analyses of bone marrow- derived MSCs Cells were uniformly negative for CD34, CD45, and positive for CD44, and CD29 ... with NIH image software Effect of simvastatin on the cell viability of bone marrowderived MSCs in vitro To examine whether simvastatin has anti-apoptotic effect on bone marrow- derived MSCs under ... Effect of simvastatin on the cell viability of bone marrow -derived MSCs in vitro In vitro, serum starvation induced bone marrow- derived MSCs apoptosis, as indicated by flow cytometry and MTT...
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Bone marrow derived mesenchymal stem cell (BM MSC) application in articular cartilage repair 10
Ngày tải lên :
10/09/2015, 09:01
... Lim B Identification of common pathways mediating differentiation of bone marrowand adipose tissue -derived human mesenchymal stem cells into three mesenchymal lineages Stem Cells 2007 Mar;25(3):750-60 ... the stem cells and/ or attract the endogenous or exogenous stem cells (endogenous from the bone marrow and exogenous by multiple intra-articular injection of the expanded autologous stem cells) ... et al The in vitro migration capacity of human bone marrow mesenchymal stem cells: comparison of chemokine and growth factor chemotactic activities Stem Cells 2007 Jul;25(7):1737-45 PubMed PMID:...
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Bone marrow derived mesenchymal stem cell (BM MSC) application in articular cartilage repair 1
Ngày tải lên :
10/09/2015, 09:10
... 1.4.5.3 Stem Cells in Articular Cartilage repair 13 iv 1.5 Types of stem cells 14 1.5.1 Embryonic stem cells (ESCs) 15 1.5.2 Induced pluripotent stem (iPS) cells 15 1.5.3 Adult stem cells ... harvest, stem cells are classified into two groups of embryonic stem cells (ESC) and adult stem cells (70) (figure 1.7 adopted from (71)) Figure 1-7 Stem cell classification and differentiation ... cell components Mesenchymal stem cells Bone marrow, peripheral blood Bone, Cartilage, tendon, adipose tissue, muscle, marrow stroma, neural cells Neural stem cells Ependymal cells, astrocytes...
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Bone marrow derived mesenchymal stem cell (BM MSC) application in articular cartilage repair 2
Ngày tải lên :
10/09/2015, 09:10
... analysis of the stem cells surface markers Harvested cells were positive for CD29, CD44, and CD90, and negative for CD14, CD31, CD45, and CD34 59 3.4.2 Prussian blue staining of SPIO-labeled ... 3.4.1 Characterization of MSCs Cells cultured from aspirated BM had characteristic of fibroblastic spindleshape morphology and flow cytometry confirmed that more than 96% of the cells were positive ... and CD90), more than 98% of cells were negative for hematopoietic markers (CD14, CD31, CD34, and CD45), and endothelial marker (CD31) (figure 3.3) 58 Figure 3-3 Flow cytometry analysis of...
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Bone marrow derived mesenchymal stem cell (BM MSC) application in articular cartilage repair 3
Ngày tải lên :
10/09/2015, 09:11
... assay showed that labeling of the MSCs did not affect the proliferation rate of the cells over time 3.4.6 Differentiation potential of labeled MSC MSCs are multipotent stem cells, which can differentiate ... Figure 3-11 Chondrogenic differentiation potential evaluation of the MSCs Upper panel shows the Alcian blue staining of unlabeled and labeled cells with different concentration of ferucarbotran ... the labeled cells 3.4.6.1 Adipogenic differentiation Qualitative evaluation of adipogenic differentiation after 21 days by Oil red O staining demonstrated that unlabeled and labeled cells exhibited...
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Bone marrow derived mesenchymal stem cell (BM MSC) application in articular cartilage repair 4
Ngày tải lên :
10/09/2015, 09:11
... indicate the defect site and arrows demonstrate the labeled stem cells) 72 Figure 3-16 MRI of the distribution of the labeled MSCs in the mini-pig knee joint The labeled cells migrated to the ... defect site, para-patellar fat and injection site of the knee joint (figure 3.15 and 3.16) 71 Figure 3-15 MR imaging of the mini-pig's femoral condyle MR imaging of the mini-pig’s femoral condyle ... healing process and the secretion of proinflammation factors associated with remodeling and repair of the injury provided the stimuli for MSC migration MRI revealed that the injected cells were in...
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Bone marrow derived mesenchymal stem cell (BM MSC) application in articular cartilage repair 6
Ngày tải lên :
10/09/2015, 09:11
... viability and chondrogenic differentiation of the labeled cells at higher concentrations Our results are in agreement with those of Kostura et al (192), but not with those of Heymer et al (205) and ... promising tool for real time evaluation and tracking of MSCs, providing data for a better understanding of the migration and localization of the injected cells 76 ... labeled cells negatively affected the osteogenic differentiation of the cells in vivo In contrast, our study showed that iron labeling did not affect the adipogenic and osteogenic potential of MSCs...
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Bone marrow derived mesenchymal stem cell (BM MSC) application in articular cartilage repair 7
Ngày tải lên :
10/09/2015, 09:11
... Grand Island, NY, US) and sub-cultured into two new flasks Cells were expanded and sub-cultured to get enough number of cells for the further experiments (passage 4) The cells were detached and ... designing a microfluidic system Microfluidic platforms are capable of mimicking some of the complexities of in vivo conditions This device provides the opportunity to culture the stem cells and injured ... capacity of self-repair Cell based therapy is one promising approach in the treatment of damaged cartilage Bone marrow (BM) derived mesenchymal stem cell (MSC) is a good candidate because of their...
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Báo cáo y học: "Sphingosine-1-phosphate promotes the differentiation of human umbilical cord mesenchymal stem cells into cardiomyocytes under the designated culturing conditions" pdf
Ngày tải lên :
10/08/2014, 05:21
... triggers differentiation of HUMSCs into cardiomyocytes and maturation of HUMSCs derived cardiomyocytes Admittedly, the detailed mechanism(s) of above effects of S1P on differentiation of and maturation ... with Regard to the Source of Fetus -Derived Stem Cells Stem cells 2007, 25:2886-2895 Wu KH, Mo XM, Zhou B, Lu SH, Yang SG, Liu YL, Han ZC: Cardiac potential of stem cells from whole human umbilical ... consisting of 500 ml of basal medium, 25 ml of fetal bovine serum (ScienCell Research Laboratories, Cat No 0025), ml of mesenchymal stem cell growth supplement (Cat No.7552) and ml of penicillin/...
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Báo cáo y học: " High efficient isolation and systematic identification of human adipose-derived mesenchymal stem cells." ppsx
Ngày tải lên :
10/08/2014, 10:20
... of mesenchymal stem cells derived from bone marrow and adipose tissue J Cell Biochem 2006, 99:1285-1297 Kern S, Eichler H, Stoeve J, Kluter H, Bieback K: Comparative analysis of mesenchymal stem ... endothelial cells enhance osteogenesis and vascularization of mesenchymal stem cells Cells Tissues Organs 2009, 190:185-193 Rodbell M: The metabolism of isolated fat cells IV Regulation of release of ... before [24] Most cells were in resting period of cell cycle agreeing with the characteristics of human bone marrow- derived mesenchymal stem cells [5] The Yang et al Journal of Biomedical Science...
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Báo cáo khoa học: "Implantation of canine umbilical cord blood-derived mesenchymal stem cells mixed with beta-tricalcium phosphate enhances osteogenesis in bone defect model dogs" ppsx
Ngày tải lên :
07/08/2014, 23:22
... of mixture of β-TCP and cells revealed significantly greater area of new bone formation than control (p ＜ 0.05) and a smaller amount of residual TCP Previous studies showed that bone marrow- derived ... human bone marrow and umbilical cord blood Stem Cells 2006, 24, 679-685 Chen L, Tredget EE, Wu PYG, Wu Y Paracrine factors of mesenchymal stem cells recruit macrophages and endothelial lineage cells ... Alternatively, a paracrine effect of canine UCB-MSCs may have enhanced new bone formation Analyses of bone marrow MSCs with real-time polymerase chain reaction and of bone marrow MSC-conditioned medium...
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Báo cáo y học: "Transcriptional profiles discriminate bone marrow-derived and synovium-derived mesenchymal stem cells" ppsx
Ngày tải lên :
09/08/2014, 07:20
... expressed in bone marrow- derived or synovium -derived cells Genes whose mRNA expression is differentially expressed in bone marrow- derived or synovium -derived cells Gene Upregulated in bone marrow- derived ... http://arthritis-research.com/content/7/6/R1304 Figure derived adherent cells In-vitro-induced differentiation of bone marrow- derived and synoviumderived adherent cells (a) Chondrogenic differentiation was evaluated ... whereas it was negative Figure derived adherent cells Immunosuppressive properties of bone marrow- derived and synoviumderived adherent cells (a) Proliferative activity of T cells in a mixed lymphocyte...
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Báo cáo y học: "HIV-1 and recombinant gp120 affect the survival and differentiation of human vessel wall-derived mesenchymal stem cells" pot
Ngày tải lên :
13/08/2014, 01:20
... smooth muscle and endothelial cells To study the effects of HIV-1 on the differentiation of these cells, the interaction of HIV-1 and recombinant gp120 on MSC differentiation to adipogenic and endothelial ... infection of human bone marrow stromal fibroblasts Blood 1990, 76:317-322 40 Wang L, Mondal D, La Russa VF, Agrawal KC: Suppression of clonogenic potential of human bone marrow mesenchymal stem cells ... HIV-1 and recombinant gp120 affect the survival and differentiation of human vessel wall -derived mesenchymal stem cells Retrovirology 2011 8:40 Submit your next manuscript to BioMed Central and...
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Báo cáo sinh học: "Autologous Transplantation of Adipose-Derived Mesenchymal Stem Cells Markedly Reduced Acute Ischemia-Reperfusion Lung Injury in a Rodent Model" potx
Ngày tải lên :
18/06/2014, 22:20
... Prevention of endotoxin-induced systemic response by bone marrow- derived mesenchymal stem cells in mice Am J Physiol Lung Cell Mol Physiol 2007, 293:L131-141 23 Salgar S, Manning E, Ruiz P, Pham S: Mesenchymal ... IR damage Figure Impact of Adipose -Derived Mesenchymal Stem Cells (ADMSC) Transplantation on the Severity of IR-Induced Lung Parenchymal Injury Number of alveolar sacs and crowded area (was defined ... Therapeutic potential of mesenchymal stem cells for severe acute lung injury Chest 2010, 138:965-972 20 Chistiakov DA: Endogenous and exogenous stem cells: a role in lung repair and use in airway...
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Báo cáo khoa học: "Transplantation of canine umbilical cord blood-derived mesenchymal stem cells in experimentally induced spinal cord injured dogs" pot
Ngày tải lên :
07/08/2014, 23:22
... ’slaminA yrotarobaL fo esU dna eraC eht rof ediuG‘ eht fo senilediug eht ot gnidrocca demrofrep saw noitagitsevni sihT yduts yrujni droc lanips latnemirepxe eht rof desu erew ,gk 95.0 ± 77.3 dehgiew ... wef a ,revewoh ;noitamrof ytivac fo egatnecrep tsellams eht detartsnomed puorg PC eht ,noitidda nI spuorg GBCU dna CSM-BCU eht ni noitamrof ytivac fo egatnecrep rellams a dnuof ew ,sllec mets detnalpsnart ... sllec detnalpsnart eht rof troppus reffo yam droc derujni eht ,droc lanips eht fo sdne lamixorp dna latsid eht ot noitcennoc rof ,slairetam ro sllec suoirav fo pu edam dloffacs ro egdirb a htiw...
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Báo cáo khoa học: " Isolation and characterization of canine umbilical cord blood-derived mesenchymal stem cells" pps
Ngày tải lên :
07/08/2014, 23:22
... Anghileri E, Andreini A, Pizzolo G, Sbarbati A, Bonetti B Induction of neural-like differentiation in human mesenchymal stem cells derived from bone marrow, fat, spleen and thymus Bone 2007, 40, ... (Figs 4E and F) Discussion Isolation and characterization of stem cells derived from various tissues and sources have been one very critical issue for stem cell therapy [12,28,33] The purpose of this ... increasing the passage number of adult stem cells often leads to a decline in the multipotent abilities [36] Human mesenchymal stem cells could be proliferated and have differentiation abilities...
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Báo cáo y học: "Phenotypic and functional abnormalities of bone marrow-derived dendritic cells in systemic lupus erythematosus" pot
Ngày tải lên :
12/08/2014, 12:20
... controls BMDCs, bone marrow- derived dendritic cells; BMCs, bone marrow cells; FLDCs, dendritic cells induced by FL; mDCs, myeloid dendritic cells; FL, FMS-like tyrosine kinase ligand; GM-CSF, granulocyte ... precursors in the bone marrow are mainly CD34+ stem cells [35] Sun and colleagues [46] recently reported that CD34+ stem cells from patients with SLE had abnormal expression of CD166 and CD123 and that ... Gershwin ME: Expansion of bone marrow IFN-alpha-producing dendritic cells in New Zealand Black (NZB) mice: high level expression of TLR9 and secretion of IFNalpha in NZB bone marrow J Immunol 2004,...
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