child and adolescent psychopharmacology
chemical thermodynamics - basic concepts and methods
... a Gibbs Function of a Reaction from Standard Gibbs Function of Formation / 286 12.5 Calculation of a Standard Gibbs Function from Standard Entropies and Standard Enthalpies / 287 Enthalpy Calculations ... 170 7.4 Pressure and Temperature Dependence of DG / 172 7.5 Useful Work and the Gibbs and Helmholtz Functions / 175 Isothermal Changes / 175 Changes at Constant Temperature and Pressure / 177 ... Statement of Lewis and Randall / 262 11.3 Thermodynamic Properties at Absolute Zero / 263 Equivalence of G and H / 263 DCP in an Isothermal Chemical Reaction / 263 Limiting Values of CP and CV / 264...
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basic statistics understanding conventional methods and modern insights jul 2009
... Cataloging-in-Publication Data Wilcox, Rand R Basic statistics : understanding conventional methods and modern insights / Rand R Wilcox p cm Includes bibliographical references and index ISBN 978-0-19-531510-3 ... Advances and Insights 9 12 19 22 25 Graphical Summaries of Data and Some Related Issues 3.1 Relative Frequencies 3.2 Histograms 3.3 Boxplots and Stem -and- Leaf Displays 3.4 Some Modern Trends and Developments ... insights and focus on methods that were developed prior to the year 1960 However, these insights have tremendous implications regarding basic principles and techniques, and so a simple description and...
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development of novel micro-embossing methods and microfluidic designs for biomedical applications
... reagents, and wash and calibration fluids) through micron-sized channels; valving; fluid mixing and isolation as desired; small volume sample metering; sample splitting and washing; and temperature ... many years and the fundamental understanding of the relationships among material properties, processing conditions and part quality has been widely investigated [Y,-J Juang, 2001, Part I and Part ... (e.g diffraction gratings, miniature lens and mirrors); automotive crash, acceleration and distance sensors; camera and watch components [Snyder, 1999]; and mechanical devices (e.g printer heads,...
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Glycoprotein Methods and Protocols - P1
... a UV monitor and collect fractions using a fraction collector and subject to the appropriate carbohydrate and antibody analyses 3.6 Analysis of Mucins Methods for the detection and analysis of ... hexose and sialic acid; membrane-based methods such as slot-blotting and staining with periodic acid-Schiff reagent; antibodies and lectins or coating methods such as the glycan detection method and ... serine proteases and esterases, including acetylcholinesterase, and should therefore be handled in a fume cupboard with extreme care! DFP is supplied in 1-g vials with a septum, and prior to dilution,...
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Glycoprotein Methods and Protocols - P2
... mammary gland, defined by antibodies to cytokeratins J Cell Sci 75, 17–33 Kiernan, J A (1990) Histological and Histochemical Methods: Theory and Practice, Pergamon, Oxford, UK Andrew, S M and Jasani, ... M and Milton, J D (1986) Lectin Methods and Protocols, Humana, Totowa, NJ Liener, I E., Sharon, N., and Goldstein, I J (1998) The Lectins: Properties, Functions, and Applications in Biology and ... such as Culling’s Handbook of Histopathological and Histochemical Techniques (including museum techniques) (36) or Kiernan’s Histological and Histochemical Methods: Theory and Practice (45) provide...
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Glycoprotein Methods and Protocols - P3
... greater sample volume, and superior washing) For quantitation and comparison across blots, a standard in the same buffer as samples should be titrated for use as a standard curve, and samples should ... detection of mucins, and two of the more popular are the anthrone assay (as both a manual and an automated procedure) (10) and the PAS reaction (11) In addition, manual and automated assays have ... using a standard curve fitted using an appropriate line of best fit; programs are available to interface with microplate readers and isotope counters that store data and compute standard curves...
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Glycoprotein Methods and Protocols - P4
... and held together by bolts tightened by hand Millipore filter paper to act as a backing for the mucosa during sectioning and subsequent manipulation of the section 2.2 Histological Staining and ... layer will be greatly reduced and discontinuous Careful handling of the cryostat sections on the slide during fixing and staining is important because excessive washing and so on can remove mucus ... adherent to rat, frog and human gastric mucosa: influence of feeding prostaglandin, N-acetyl-cysteine and other agents Clin Sci 63, 187–195 Bell, A E., Allen, A., Morris, E R., and Ross-Murphy, S...
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Glycoprotein Methods and Protocols - P5
... PhosphorImager The identification of mucin precursors and mature mucins using polypeptide-specific antisera and SDS-PAGE is elaborated in Chapters 20 and 21, and in several references (1,4–11) The precursor ... Strous, G J and Dekker, J (1992) Mucin-type glycoproteins Crit Rev Biochem Mol Biol 27, 57–92 Van Klinken, B J W., Dekker, J., Büller, H A., and Einerhand, A W C (1995) Mucin gene structure and expression ... Einerhand, A W C., and Dekker, J (1995) Biosynthesis of intestinal mucins: MUC1, MUC2, MUC3, and more Biochem Soc Trans 23, 814–818 11 Van Klinken, B J W., Einerhand, A W C., Büller, H A., and Dekker,...
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Glycoprotein Methods and Protocols - P6
... a small gel, use 1.6 g of agarose and 160 mL of buffer, and for a large gel, use 2.8 g of agarose and 280 mL of buffer Leave to cool before pouring (hand hot) and insert well-forming comb (see ... are 1.5 mm thick and cm wide There is a compromise between band broadness and amount of sample to be loaded Larger amounts of sample tend to yield poorer quality data; i.e., the bands become more ... by agarose gel electrophoresis and Western blots of the gels can be probed with lectins and antibodies Using this methodology, we have shown that with pooling and rechromatography it is possible...
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Glycoprotein Methods and Protocols - P7
... Kadler, K E., and Chapman J A (1985) Mica sandwhich technique for preparing macromolecules for rotary shadowing J Ultrastruct Res 91, 66–76 14 Kawahara K and Tanford C (1966) Viscosity and density ... (Fullerton, CA) SW 40Ti swing-out rotor, and finally unloading and analysis of the tube contents Measure out 18 mL of M GuHCl into one chamber of the gradient maker and an equal volume of M GuHCl into ... into 0.5-mL fractions, and these were analyzed for reactivity with antisera for the MUC5AC (᭹) and MUC5B (᭺) mucins The MUC5B mucin is more polydisperse than the MUC5AC mucin and has molecules of...
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Glycoprotein Methods and Protocols - P8
... 114 Yan and Packer chromatography, and leads to a significantly lower recovery of all the amino acids, especially serine and threonine Strong acid, high temperature, and short time acid ... at least for pump and column equilibration with the initial composition of the mobile phase Figures and show typical chromatograms of the separation of amino acid standards and hydrolyzed bovine ... samples and Dr Andrew Gooley and Prof Keith Williams for their support References Yan, J X., Wilkins, M R., Ou, K., Gooley, A A., Williams, K L., Sanchez, J.-C., Golaz, O., Pasquali, C., and Hochstrasser,...
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Glycoprotein Methods and Protocols - P9
... Notes and 8) and the length of sequence obtained is dependent on the number and distribution of proline residues Proline cleaves with reduced efficiency in Edman sequencing (Fig 1, vessel 1), and ... I., Kirk, M., Barnes, S., Lowary, T L., and Hart, G W (1996) Selective detection and site-analysis of O-GlcNAc-modified glycopeptides by β-elimination and tandem electrospray mass spectrometry Anal ... PerSeptive Biosystems and Beckman supply this product Quantitation of glycopeptides is quite difficult, and we recommend that 10% of the sample be first sequenced using the standard program prior...
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Glycoprotein Methods and Protocols - P10
... tandem repeat domain and overlapping each other by six amino acids (16), and the other used octamers overlapping each other by seven amino acids (17) Twelve antibodies were analyzed in total and ... and methanol (two times) The efficiency of the cleaning procedure should be tested by repeating steps and Absorbance levels above background indicate that antibody remains bound to the pins and ... Chromatography (see Notes 12–15 and Fig 3) Prepare Sepharose-peptide affinity matrices and pack columns according to the manufacturer’s instructions (see Notes 12 and 13) Equilibrate the columns...
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Glycoprotein Methods and Protocols - P11
... lysates and media Nonspecific bands may be present and can be eliminated from consideration using the pSVL vector control lanes for comparison (Fig 2A,B, lanes and 3) The sizes of specific bands ... polymerase buffer, 2.5 mM MgCl2, 400 µM dNTPs, 200 ng of primers and or and 4, ng of template DNA (with only primers and 4), and U of Taq polymerase Perform a PCR program in a suitable thermal ... polymerase, and incubate the reaction at 72˚C to allow extension of the linked templates Add µg each of primers and 6, and continue the PCR with 30 cycles at 94˚C for 30 s, 50˚C for and 72˚C for...
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Glycoprotein Methods and Protocols - P12
... gas Program the oven temperature from 150 to 230°C and 3°C/min and then 230 to 250°C at 5°C/min Use injector and detector temperatures of 240 and 250°C, respectively Use an injection volume of ... Nakamura, M., and Ohkura, Y (1987) Fluorometric high performance liquid chromatography of N-acetyl- and N-glycolyl neuraminic acids and its application to their microdetermination in human and animal ... oven temperature from 120 to 240°C at 2°C/min Use injector and detector temperatures of 240 and 250°C, respectively 168 168 Michalski and Capon Fig GLC separation of trimethylsilylated deriviatives...
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Glycoprotein Methods and Protocols - P13
... 6.0, and load the sample carefully and directly to the center of the column, drop wise (see Note 3) Centrifuge for at 1100g and collect the excluded O-Glycanase O-Linked Chain Release and Fractionation ... (3 × 0.5 cm column) and elute with three column vol of HPLC-grade water Collect the effluent and eluent and then pool (2.5-mL volume) Load onto a PD10-Sephadex GM25 column and elute with HPLC-grade ... Davies, M J and Hounsell, E F (1998) HPLC and HPAEC of oligosaccharides and glycopeptides, in Glycoscience Protocols (Hounsell, E F., ed.), Humana, Totowa, NJ, pp 79–100 14 Kakehi, K., and Honda,...
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Glycoprotein Methods and Protocols - P14
... was introduced in the 1970s and rapidly received a large application for analyzing the sequence of N-acetyllactosamine- and oligomannosidic-type glycans Originally, and before the development ... H-2, H-4, H-5 and H-6' Analysis of Mucin-Type O-Linked Oligosaccharides 193 Fig Strategy of elucidation of oligosaccharide primary structure atoms; Gal H-3 and H-4 atoms; Fuc H-5 and H-6 atoms; ... at ~ 1.2 ppm The α-1,2 (H), α-1,3 (Lex), and α-1,4 (Lea) linkages are deduced from the position of H-1, H-5, and H-6 resonances For instance, the Lex and Lea epitopes can be characterized on...
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Glycoprotein Methods and Protocols - P15
... hygroscopic and must be thoroughly dried before weighing and making up as standards Standards of mM each of 2-, 3-, and 6-sulfo-Nacetylglucosamine in water may be aliquoted and stored at –20°C, and used ... chloride (Cl–), sulfate (HSO4–), and phosphate (H2PO3–) ions (see also Note 11) Chromatogram displays 10 nmol each of phosphate and sulfate, and trace amounts of chloride and acetate Note the difference ... 212 Harrison and Packer Materials 2.1 Solvents and Reagents Water, carbonate-free (see Note 1), and sparged with a constant flow of argon 0.1 M NaOH, made up with decarbonated water, and similarly...
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Glycoprotein Methods and Protocols - P16
... Subheading 2., item 5e at 37°C and 5% CO2 Caco-2 and A431 cells are passaged (trypsinised) and split 1:4 and the medium is changed every to d LS174T cells are split 1:2 and the medium changed daily ... only MUC and MUC2 at early passage, but at later passage and in later premalignant and malignant stages, they show de novo expression of MUC5AC, MUC5B, and MUC6 (Corfield, Myerscough, and Paraskeva, ... clumps of cells and replate under standard culture conditions Reattachment of cells may take several days, and during medium changing, any floating clumps of cells must be centrifuged and replated...
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Glycoprotein Methods and Protocols - P17
... After incubation, remove the medium from the central well and wash the tissue and dish with mL of PBS Pool the medium and washings, and dialyze against three changes of L of M guanidine hydrochloride ... Electrophoresis and Vacuum Blotting (see Notes 16 and 17) Mix samples containing 10–500 mg mucin or >10,000cpm with 50 mL sample buffer and load onto horizontal 0.8–1% agarose gels Run Rainbow markers and ... to –70°C and leave for 45 at –70°C Return to room temperature for centrifugation at 12,000g for 20 and collect the pellet 3.3.5 Identification and Removal of Proteoglycans (see Notes 21 and 22)...
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