chemical modification of amino acids
Microbial Production of Amino Acids in Japan
... soybea1ns contain lower amounts of lysine, which is one of Microbial Production of Amino Acids in Japan 77 the essential amino acids for those livestocks The estimated amount of l-lysine produced in ... successful introduction of the technology, various methods were searched for and developed for microbial production of other amino acids Today a whole array of amino acids are produced by microbial ... various amounts of sodium pyruvate, g of ammonium acetate, 0.6 g of pyrocatechol, 0.2 g of sodium sulfite, 0.1 g of EDTA, and cells harvested from 100 ml of broth, in a total volume of 100 ml.The...
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Báo cáo khóa học: Vertical-scanning mutagenesis of amino acids in a model N-myristoylation motif reveals the major amino-terminal sequence requirements for protein N-myristoylation ppt
... study, to examine the effect of the combination of amino acids at positions and on protein N-myristoylation, sequential vertical-scanning mutagenesis of the amino acids at positions and in a model ... the combination of amino acids at positions and might be a critical determinant for protein Nmyristoylation In the present study, to examine the effect of the combination of amino acids at positions ... addition to the combination of amino acids at positions and 6, it was demonstrated that the combination of amino acids at positions and also affects the susceptibility of the protein to protein...
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Báo cáo khoa học: Identification of amino acids in antiplasmin involved in its noncovalent ‘lysine-binding-site’-dependent interaction with plasmin pptx
... half-lives of plasmin in these experiments (also before 50% of the plasmin activity was inhibited) Computer model of the C-terminal 40 amino acids in antiplasmin A computer model of the C-terminal 40 amino ... absence of 6-aminohexanoic acid) The reactions between ‘native’ human antiplasmin and plasmin in the presence or absence of 6-aminohexanoic acid were also studied for comparison All variants of antiplasmin ... in detail by constructing seven single-site mutants of antiplasmin Fig Computer model of the C-terminal 40 amino acids in antiplasmin Some of the residues are labelled to facilitate viewing Ó...
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Guanidine catalyzed enantioselective mannich reaction towards the synthesis of amino acids
... TOWARDS THE SYNTHESIS OF -AMINO ACIDS PAN YUANHANG (BSc., Zhejiang University) A THESIS SUBMITTED FOR THE DEGREE OF DOCTOR OF PHILOSOPHY DEPARTMENT OF CHEMISTRY NATIONAL UNIVERSITY OF SINGAPORE To ... reaction of phenylacetate thioesters by soft enolization Scheme 2.3 Cross-aldol reaction of isatins and ketones Scheme 2.4 Synthesis of MAHTs started from diethyl malonate Scheme 2.5 Synthesis of N-sulfonyl ... Scheme 4.9 Proposed mechanism of deacylation of Mannich product 129g Scheme 4.10 Proposed mechanism of decarboxylation of Mannich product 129g Scheme 4.11 Cleavage of N-Eoc imine under acidic condition...
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Biodegradable Polymers Composed of Naturally Occurring α-Amino Acids
... practical applications of this very promising family of biodegradable polymers, it is indispensable to carry out a comprehensive study of the interaction of AABBPs of various chemical compositions ... M (1961) Chemistry of the Amino Acids, John Wiley & Sons, Inc., New York, London Zavradashvili, N (2008) New functional biodegradable poly(ester amide)s composed of α -amino acids and their potential ... the termination of the chain growth, whereas in case of phosgene no monofunctional compound is formed since it hydrolyses with the liberation of CO2 and HCl 5.2.3.4 Transformation of AABBPs All...
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Tài liệu Báo cáo khoa học: The mechanism of a-proton isotope exchange in amino acids catalysed by tyrosine phenol-lyase doc
... side chain of any bound amino acid The lability of the a-proton observed for a large number of amino acids [5] under the action of TPL gives evidence for the orthogonal orientation of the a-proton ... with respect to the cofactor plane [17], and shows that the pattern of binding is the same for a variety of amino acids It has been established [5] that for a number of amino acid inhibitors ... References Konnikova, A.S., Dobbert, N.N & Braunstein, A.E (1947) Labilization of a-hydrogen of amino acids under the action of aminoferase Biokhimia 12, 556–568 (in Russian) Ó FEBS 2004 Esaki, N., Nakayuma,...
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Báo cáo khoa học: The ATPase activities of sulfonylurea receptor 2A and sulfonylurea receptor 2B are influenced by the C-terminal 42 amino acids doc
... activities of NBD2A and NBD2B could result from an inhibitory effect of the C-terminal 42 amino acids of NBD2B or a stimulatory effect of the equivalent amino acids of NBD2A To determine which of these ... last 42 amino acids Figure 2A and Table show that the ATPase activity of NBD2-DC was greater than that of SUR2B but similar to that of NBD2A, favoring the idea that the last 42 amino acids of NBD2B ... equation using a Km of 460 lM, a Vmax of 2.52 nmol PiÆminÆmg)1 and an offset of 0.1 nmol PiÆminÆmg)1 for SUR2A, and a Km of 41 lM, a Vmax of 0.73nmol PiÆminÆmg)1 and an offset of 0.05 nmol PiÆminÆmg)1...
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Báo cáo khoa học: Control of mammalian gene expression by amino acids, especially glutamine potx
... involved in the action of amino acids on gene expression Amino acid(s) manipulations Factor(s) implied Inhibiting effect resulting from the presence of amino acids Pooled amino acids deprivation Increased ... presence of amino acid(s) resulted in inhibition of the DNA binding of the involved transcription factors By contrast, the presence of amino acid may also result in stimulation of the DNA binding of ... effects of different amino acids (except glutamine), together with the identified transcription factors and the responsive elements involved Most of the data concern the inhibitory effect of amino acids...
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Báo cáo khoa học: Amino acids at the N- and C-termini of human glutamate carboxypeptidase II are required for enzymatic activity and proper folding pptx
... to consist of six domains: the N-terminal cytoplasmic tail (amino acids 1–18), the helical transmembrane region (amino acids 19–43), and four extracellular domains spanning amino acids 44–150 ... recovered, apparently as a result of the blocking of the N-terminal amino acid Subsequent Western blot analysis, exploiting the 7E11 mAb recognizing the first six amino acids of the full-length GCPII [31], ... removal of as few as 15 amino acids from the C-terminus completely abolished NAAG-hydrolyzing activity (the 44/736 variant), and the C-terminal extension (addition of the V5-His tag in the case of...
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Báo cáo khoa học: Transport of taurocholate by mutants of negatively charged amino acids, cysteines, and threonines of the rat liver sodium-dependent taurocholate cotransporting polypeptide Ntcp docx
... importance of negatively charged amino acids for sodium-coupled substrate cotransport or exchange [12,13,16–18] Mutations of all conserved and two nonconserved negatively charged amino acids to ... mutagenesis of Ntcp (Eur J Biochem 270) 1121 Fig Topology model of rat Ntcp based on hydropathy analysis of the amino acid sequence (according to [1]) Transmembrane domains are symbolized as blocks of amino ... of amino acids Mutated amino acids are highlighted in gray The resulting mutants are shown in boxes, with deletions indicated (del) Fig Mutations of negatively charged conserved amino acids alters...
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Báo cáo Y học: Amino acids 3–13 and amino acids in and flanking the 23FxxLF27 motif modulate the interaction between the N-terminal and ligand-binding domain of the androgen receptor pdf
... obtained by exchange of a 1.8-kb HindIII fragment of pSVAR3 with the corresponding fragment of pGAD3D3–37 For construction of pSVAR.NTDD3-37, a 1.7-kb HindIII/Asp718 fragment of pSVAR.NTDwt was ... of three independent experiments Fold induction is shown to the right of each bar and represents the ratio of activities determined in the presence and absence of R1881 (C) Western analysis of ... substitutions of amino acids flanking 23FQNLF27 reduced the binding to AR LBD Most prominent inhibitory effects were found for amino acid residues directly flanking 23FQNLF27 Note that expression levels of...
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Báo cáo khoa học: Arg143 and Lys192 of the human mast cell chymase mediate the preference for acidic amino acids in position P2¢ of substrates pdf
... aromatic amino acids (Phe, Tyr, and Trp); negatively charged amino acids (Asp and Glu); positively charged amino acids (Lys and Arg); small aliphatic amino acids (Gly and Ala); larger aliphatic amino ... negatively charged amino acids, and instead preferred aliphatic or noncharged amino acids at this position The identification of Arg143 and Lys192 as P2¢ preference-determining amino acids facilitates ... side chains of substrates, but not when the negative charge is situated at a C-terminal carboxyl group of a P2¢ amino acid of Fig Analysis of cleavage specificity by the use of new types of recombinant...
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Báo cáo khoa học: Chromophore attachment in phycocyanin Functional amino acids of phycocyanobilin – a-phycocyanin lyase and evidence for chromophore binding doc
... affect the stability of the proteins [28] The C terminus of CpcF shows only little homology for as much as 50 amino acids A truncation by 53 amino acids reduced the solubility of the protein, possibly ... SDS ⁄ PAGE Chemical modifications of amino acids Chemical modifications of arginine, lysine, carboxyl groups, tryptophan and histidine were performed as described before [28] The number of essential ... terminus of CpcF showed more homology: a 10 amino acid truncation reduced the activity to 26%, and it was lost completely when 20 amino acids were truncated Among the 10 N-terminal amino acids, ...
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Báo cáo khoa học: Assimilation of excess ammonium into amino acids and nitrogen translocation in Arabidopsis thaliana – roles of glutamate synthases and carbamoylphosphate synthetase in leaves ppt
... [2,16,17] The high levels of glutamine and glutamate (nitrogen-rich ve-carbon amino acids) and asparagine and aspartate (four-carbon amino acids) (up to 80% of the total amino acids) (Fig 3) 4068 te ... mixture consisted of 0.4 ng of genomic DNA isolated from rosette leaves, 10 pmol of forward primer, 10 pmol of reverse primer and 0.2 units of Taq polymerase in a total volume of 25 lL The following ... T-DNA insertion in exon 13 about 50 amino acids upstream of the FMN-binding domain (Fig 2B) The GLT T-DNA mutant contained about 20% of the wild-type level of GLT mRNA (Fig 2D) The carB mutant...
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Báo cáo khoa học: D-Amino acids in the brain: the biochemistry of brain serine racemase potx
... first four amino acids of the barley serine racemase and the final 88 amino acids of the Pyrobaculum serine racemase are omitted for clarity Alignment was performed using the CLUSTAL software 3540 ... the binding of PICK1 on serine racemase activity Therefore, biochemical characterization of the role of the interaction of serine racemase and PICK1 is needed to judge the importance of these observations ... carboxy-terminal end of the polypeptide chain, disrupts its interaction with PDZ4 ⁄ PDZ5 of GRIP, it is tempting to speculate that phosphorylation of Ser336 of human serine racemase or of Thr336 of mouse...
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Báo cáo khoa học: Charging of tRNA with non-natural amino acids at high pressure potx
... Charging of tRNA with non-natural amino acids M Giel-Pietraszuk and J Barciszewski Table Yield of aminoacylation of tRNA with different amino acids pmole amino acid per 1600 pmole tRNA Amino acid ... and tRNAVal charging with a series of natural amino acids showed that the best yields were obtained for aromatic amino acids, but aminoacylation using amino acids with an aliphatic side chain ... noncognate amino acids in the aminoacylation of CoA, and in the acylation of mini helix of RNA [32] The equilibrium of CoA acylation was shifted towards an aa-S-CoA formation [33] In the case of high-pressure...
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báo cáo hóa học:" Minireview: Transaminases for the synthesis of enantiopure beta-amino acids" doc
... β -amino acids including (1) the resolution of racemic β -amino acids, (2) the use of naturally occurring chiral α -amino acids, and (3) asymmetric synthesis (Liu and Sibi 2002) As resolutions of ... application of transaminases for the synthesis of β -amino acids Of particular interest are methods that can be used at small scale compatible with microtiter plates Enantiopure β -amino acids represent ... variety of aliphatic and aromatic β -amino acids as amino donors with good accuracy Furthermore, by using both enantiomers of an amino donor separately, information on the enantiopreference of the...
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Báo cáo y học: " Effects of carbohydrate, branched-chain amino acids, and arginine in recovery period on the subsequent performance in wrestlers" potx
... WJ: The stimulus-secretion coupling of amino acid-induced insulin release: insulinotropic action of br anched-chain amino acids at physiological concentr ations of glucose and glutamine Eur J Clin ... anched-chain amino acids prolong exercise dur ing heat stress in men and women Med Sci Sports Exerc 1998, 30:83-91 61 Davis JM, Welsh RS, De Volve KL, Alderson NA: Effects of br anched-chain 33 amino acids ... Takehana K: Phar macological activities of br anched-chain amino acids: augmentation of albumin synthesis in liver and improvement of glucose metabolism in skeletal muscle Hepatol Res 2004, 30S:19-24...
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Báo cáo y học: " Potential therapeutic effects of branched-chain amino acids supplementation on resistance exercise-based muscle damage in humans" pps
... time of muscle soreness induced by exercise Nosaka et al.[3] 900 actions (30 min) of arm curl with 1.80 to 3.44 kg of range of workload BCAA-enriched amino acid mixture (60% of the essential amino ... concepts of muscle damage and its biochemical markers followed by evidences of effects of BCAA supplementation on RE-induced muscle damage in humans Discussion Cellular responses and biochemical ... Concerning the time-frame of supplementation, Nosaka et al [3] evaluated the effects on muscle damage supplementing an amino acid mixture (BCAA-enriched; 60% of essential amino acids) 30 minutes before,...
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Báo cáo y học: "Cerebral net exchange of large neutral amino acids after lipopolysaccharide infusion in healthy humans" potx
... decrease was associated with Table Cerebral delivery of large neutral amino acids Table Cerebral net exchange of large neutral amino acids Amino acid Cerebral delivery μmol/ 100 g/min Baseline ... Accordingly, the plasma concentrations of amino acids at baseline, measured in the present study, were comparable to whole-blood concentrations of amino acids in humans reported previously [17] ... transport of amino acids J Nutr 2006, 136:218S-226S 30 Yudkoff M, Daikhin Y, Nissim I, Horyn O, Luhovyy B, Lazarow A, Nissim I: Brain amino acid requirements and toxicity: the example of leucine...
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